NO300508B1 - Biologisk renkultur av Bacillus thuringiensis-bakterie, krystallinsk, parasporalt proteintoksin erholdt fra denne, insekticidpreparat som inneholder dette, samt anvendelse av disse til bekjempelse av insekter - Google Patents
Biologisk renkultur av Bacillus thuringiensis-bakterie, krystallinsk, parasporalt proteintoksin erholdt fra denne, insekticidpreparat som inneholder dette, samt anvendelse av disse til bekjempelse av insekter Download PDFInfo
- Publication number
- NO300508B1 NO300508B1 NO903378A NO903378A NO300508B1 NO 300508 B1 NO300508 B1 NO 300508B1 NO 903378 A NO903378 A NO 903378A NO 903378 A NO903378 A NO 903378A NO 300508 B1 NO300508 B1 NO 300508B1
- Authority
- NO
- Norway
- Prior art keywords
- bacillus thuringiensis
- ncimb
- crystalline
- toxin
- donegani
- Prior art date
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 241000193388 Bacillus thuringiensis Species 0.000 title claims abstract description 19
- 231100000654 protein toxin Toxicity 0.000 title claims description 13
- 101000664393 Bacillus thuringiensis subsp. finitimus S-layer protein Proteins 0.000 title claims description 12
- 241000238631 Hexapoda Species 0.000 title claims description 12
- 239000002917 insecticide Substances 0.000 title claims description 8
- 231100000765 toxin Toxicity 0.000 claims abstract description 30
- 239000003053 toxin Substances 0.000 claims abstract description 30
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 20
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 17
- 241000254173 Coleoptera Species 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 230000009885 systemic effect Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 230000009089 cytolysis Effects 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 239000012265 solid product Substances 0.000 claims description 3
- 108700012359 toxins Proteins 0.000 description 28
- 230000000694 effects Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000258916 Leptinotarsa decemlineata Species 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000006916 nutrient agar Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- 206010011906 Death Diseases 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 241000256250 Spodoptera littoralis Species 0.000 description 2
- 241000254109 Tenebrio molitor Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000256111 Aedes <genus> Species 0.000 description 1
- 108010082340 Arginine deiminase Proteins 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000630736 Ephestia Species 0.000 description 1
- 241000122098 Ephestia kuehniella Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101710182532 Toxin a Proteins 0.000 description 1
- 241000254112 Tribolium confusum Species 0.000 description 1
- 239000012963 UV stabilizer Substances 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002318 adhesion promoter Substances 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229940056360 penicillin g Drugs 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002623 sporogenic effect Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 235000011845 white flour Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/075—Bacillus thuringiensis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT8921412A IT1231766B (it) | 1989-08-01 | 1989-08-01 | Bacillus thuringiensis var. donegani, preparato o tossina da esso ottenuta aventi attivita' insetticida contro i coleotteri. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO903378D0 NO903378D0 (no) | 1990-07-31 |
| NO903378L NO903378L (no) | 1991-02-04 |
| NO300508B1 true NO300508B1 (no) | 1997-06-09 |
Family
ID=11181378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO903378A NO300508B1 (no) | 1989-08-01 | 1990-07-31 | Biologisk renkultur av Bacillus thuringiensis-bakterie, krystallinsk, parasporalt proteintoksin erholdt fra denne, insekticidpreparat som inneholder dette, samt anvendelse av disse til bekjempelse av insekter |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US5120536A (hu) |
| EP (1) | EP0411582B1 (hu) |
| JP (1) | JPH0380074A (hu) |
| AT (1) | ATE128186T1 (hu) |
| AU (1) | AU636633B2 (hu) |
| BR (1) | BR9003738A (hu) |
| DE (1) | DE69022509T2 (hu) |
| DK (1) | DK0411582T3 (hu) |
| ES (1) | ES2077614T3 (hu) |
| HU (1) | HU210680B (hu) |
| IL (1) | IL95224A (hu) |
| IT (1) | IT1231766B (hu) |
| NO (1) | NO300508B1 (hu) |
| PL (1) | PL165207B1 (hu) |
| RU (1) | RU2048099C1 (hu) |
| ZA (1) | ZA905973B (hu) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK141790D0 (da) * | 1990-06-08 | 1990-06-08 | Novo Nordisk As | Middel |
| GB9023735D0 (en) * | 1990-11-01 | 1990-12-12 | Ici Plc | Bacterial strain |
| EP0602064B1 (en) * | 1991-08-02 | 1998-12-16 | Mycogen Corporation | Novel microorganism and insecticide |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3346138A1 (de) * | 1983-12-21 | 1985-07-11 | Boehringer Mannheim Gmbh, 6800 Mannheim | Bacillus thuringiensis var. tenebrionis sowie ein insektizid wirkendes, hieraus erhaeltliches praeparat bzw. toxin sowie deren verwendung zur bekaempfung von coleoptera |
| US4764372A (en) * | 1985-03-22 | 1988-08-16 | Mycogen Corporation | Compositions containing bacillus thuringiensis toxin toxic to beetles of the order coleoptera, and uses thereof |
| US4771131A (en) * | 1985-08-16 | 1988-09-13 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene encoding a protein toxic to beetles of the order Coleoptera |
| US4865981A (en) * | 1985-08-16 | 1989-09-12 | Mycogen Corporation | Cloning and expression of Bacillus Thuringiensis toxin gene toxic to bettles of the order coleoptera |
| US4950471A (en) * | 1987-05-18 | 1990-08-21 | The United States Of America As Represented By The Secretary Of Agriculture | Acetate selected bacillus thuringiensis and the method of use |
| US4910016A (en) * | 1987-08-03 | 1990-03-20 | Mycogen Corporation | Novel Bacillus thuringiensis isolate |
| US4948734A (en) * | 1987-08-12 | 1990-08-14 | Mycogen Corporation | Novel isolates of bacillus thuringiensis having activity against nematodes |
| US4849217A (en) * | 1987-11-19 | 1989-07-18 | Mycogen Corporation | Novel isolates of bacilus thuringiensis having activity against the alfalfa weevil, hypera brunneipennis |
| US4966765A (en) * | 1988-02-23 | 1990-10-30 | Mycogen Corporation | Novel coleopteran-active Bacillus thuringiensis isolate |
| IT1240335B (it) * | 1990-03-21 | 1993-12-07 | Ministero Dell'universita' E Della Ricerca Scientifica E Tecnologi | Composizioni insetticide a base di bacillus thuringiensis |
-
1989
- 1989-08-01 IT IT8921412A patent/IT1231766B/it active
-
1990
- 1990-07-30 IL IL9522490A patent/IL95224A/en not_active IP Right Cessation
- 1990-07-30 ZA ZA905973A patent/ZA905973B/xx unknown
- 1990-07-30 AU AU59984/90A patent/AU636633B2/en not_active Ceased
- 1990-07-31 DE DE69022509T patent/DE69022509T2/de not_active Expired - Fee Related
- 1990-07-31 RU SU904830849A patent/RU2048099C1/ru active
- 1990-07-31 DK DK90114708.2T patent/DK0411582T3/da active
- 1990-07-31 HU HU904691A patent/HU210680B/hu not_active IP Right Cessation
- 1990-07-31 EP EP90114708A patent/EP0411582B1/en not_active Expired - Lifetime
- 1990-07-31 AT AT90114708T patent/ATE128186T1/de not_active IP Right Cessation
- 1990-07-31 BR BR909003738A patent/BR9003738A/pt not_active Application Discontinuation
- 1990-07-31 US US07/560,848 patent/US5120536A/en not_active Expired - Fee Related
- 1990-07-31 PL PL90286279A patent/PL165207B1/pl unknown
- 1990-07-31 NO NO903378A patent/NO300508B1/no not_active IP Right Cessation
- 1990-07-31 ES ES90114708T patent/ES2077614T3/es not_active Expired - Lifetime
- 1990-08-01 JP JP2204942A patent/JPH0380074A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| PL286279A1 (en) | 1991-12-02 |
| BR9003738A (pt) | 1991-09-03 |
| EP0411582B1 (en) | 1995-09-20 |
| IT8921412A0 (it) | 1989-08-01 |
| NO903378D0 (no) | 1990-07-31 |
| EP0411582A1 (en) | 1991-02-06 |
| RU2048099C1 (ru) | 1995-11-20 |
| DE69022509T2 (de) | 1996-03-14 |
| AU5998490A (en) | 1991-02-07 |
| IL95224A0 (en) | 1991-06-10 |
| AU636633B2 (en) | 1993-05-06 |
| HU210680B (en) | 1995-06-28 |
| ES2077614T3 (es) | 1995-12-01 |
| DE69022509D1 (de) | 1995-10-26 |
| DK0411582T3 (da) | 1995-11-06 |
| IT1231766B (it) | 1991-12-21 |
| ZA905973B (en) | 1991-05-29 |
| US5120536A (en) | 1992-06-09 |
| PL165207B1 (pl) | 1994-11-30 |
| HUT58789A (en) | 1992-03-30 |
| IL95224A (en) | 1995-01-24 |
| JPH0380074A (ja) | 1991-04-04 |
| ATE128186T1 (de) | 1995-10-15 |
| NO903378L (no) | 1991-02-04 |
| HU904691D0 (en) | 1991-01-28 |
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