NO175215B - - Google Patents
Info
- Publication number
- NO175215B NO175215B NO852433A NO852433A NO175215B NO 175215 B NO175215 B NO 175215B NO 852433 A NO852433 A NO 852433A NO 852433 A NO852433 A NO 852433A NO 175215 B NO175215 B NO 175215B
- Authority
- NO
- Norway
- Prior art keywords
- gene
- dna
- bmnpv
- structural gene
- upstream
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 247
- 108020004414 DNA Proteins 0.000 claims abstract description 119
- 239000012634 fragment Substances 0.000 claims abstract description 106
- 241000409811 Bombyx mori nucleopolyhedrovirus Species 0.000 claims abstract description 90
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 61
- 238000004519 manufacturing process Methods 0.000 claims abstract description 47
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 39
- 241000700605 Viruses Species 0.000 claims abstract description 32
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims abstract description 26
- 108091081024 Start codon Proteins 0.000 claims abstract description 16
- 238000005215 recombination Methods 0.000 claims abstract description 12
- 230000006798 recombination Effects 0.000 claims abstract description 12
- 102000053602 DNA Human genes 0.000 claims abstract description 6
- 241000255789 Bombyx mori Species 0.000 claims description 30
- 239000013598 vector Substances 0.000 claims description 15
- 108020004511 Recombinant DNA Proteins 0.000 claims description 12
- 238000012546 transfer Methods 0.000 claims description 8
- 238000001890 transfection Methods 0.000 claims description 6
- 230000000644 propagated effect Effects 0.000 claims description 5
- 239000000126 substance Substances 0.000 abstract description 9
- 210000004748 cultured cell Anatomy 0.000 abstract description 5
- 230000001902 propagating effect Effects 0.000 abstract description 3
- 239000013612 plasmid Substances 0.000 description 65
- 239000000047 product Substances 0.000 description 55
- 210000004027 cell Anatomy 0.000 description 32
- 108091008146 restriction endonucleases Proteins 0.000 description 17
- 108020005202 Viral DNA Proteins 0.000 description 15
- 238000003776 cleavage reaction Methods 0.000 description 12
- 239000002609 medium Substances 0.000 description 12
- 230000007017 scission Effects 0.000 description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 description 11
- 230000003612 virological effect Effects 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 241001646716 Escherichia coli K-12 Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 238000010276 construction Methods 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 108020005038 Terminator Codon Proteins 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 208000003322 Coinfection Diseases 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 108020004638 Circular DNA Proteins 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 3
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 3
- 239000004141 Sodium laurylsulphate Substances 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 229940079322 interferon Drugs 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 108700026220 vif Genes Proteins 0.000 description 3
- 125000002103 4,4'-dimethoxytriphenylmethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)(C1=C([H])C([H])=C(OC([H])([H])[H])C([H])=C1[H])C1=C([H])C([H])=C(OC([H])([H])[H])C([H])=C1[H] 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 108010047761 Interferon-alpha Proteins 0.000 description 2
- 102000006992 Interferon-alpha Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 241000711975 Vesicular stomatitis virus Species 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000120 cytopathologic effect Effects 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- VKIGAWAEXPTIOL-UHFFFAOYSA-N 2-hydroxyhexanenitrile Chemical compound CCCCC(O)C#N VKIGAWAEXPTIOL-UHFFFAOYSA-N 0.000 description 1
- SFYDWLYPIXHPML-UHFFFAOYSA-N 3-nitro-1-(2,4,6-trimethylphenyl)sulfonyl-1,2,4-triazole Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)N1N=C([N+]([O-])=O)N=C1 SFYDWLYPIXHPML-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241001203868 Autographa californica Species 0.000 description 1
- 241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- ZLZUNCWDVNGVAQ-UHFFFAOYSA-N C1(=C(C(=CC(=C1)C)C)S(=O)(=O)N1N=N[C-]=C1[N+](=O)[O-])C Chemical compound C1(=C(C(=CC(=C1)C)C)S(=O)(=O)N1N=N[C-]=C1[N+](=O)[O-])C ZLZUNCWDVNGVAQ-UHFFFAOYSA-N 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 1
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101000959820 Homo sapiens Interferon alpha-1/13 Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 101150103227 IFN gene Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052792 caesium Inorganic materials 0.000 description 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000012461 cellulose resin Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940047120 colony stimulating factors Drugs 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006642 detritylation reaction Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 108010092809 exonuclease Bal 31 Proteins 0.000 description 1
- 210000002468 fat body Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000005252 hepatitis A Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229960003971 influenza vaccine Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- YMWUJEATGCHHMB-UHFFFAOYSA-N methylene chloride Substances ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920005990 polystyrene resin Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- OENLEHTYJXMVBG-UHFFFAOYSA-N pyridine;hydrate Chemical compound [OH-].C1=CC=[NH+]C=C1 OENLEHTYJXMVBG-UHFFFAOYSA-N 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- AVBGNFCMKJOFIN-UHFFFAOYSA-N triethylammonium acetate Chemical compound CC(O)=O.CCN(CC)CC AVBGNFCMKJOFIN-UHFFFAOYSA-N 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Video Image Reproduction Devices For Color Tv Systems (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Peptides Or Proteins (AREA)
- Casting Or Compression Moulding Of Plastics Or The Like (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59128215A JPS619288A (ja) | 1984-06-21 | 1984-06-21 | ペプチド類の製法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO852433L NO852433L (no) | 1985-12-23 |
| NO175215B true NO175215B (de) | 1994-06-06 |
| NO175215C NO175215C (no) | 1994-09-14 |
Family
ID=14979338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO852433A NO175215C (no) | 1984-06-21 | 1985-06-17 | Fremgangsmåte for fremstilling av et genprodukt i Bombyx mori eller i celler derav |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US5118616A (de) |
| EP (1) | EP0175852B1 (de) |
| JP (1) | JPS619288A (de) |
| KR (1) | KR930000273B1 (de) |
| AT (1) | ATE71655T1 (de) |
| AU (1) | AU588236B2 (de) |
| BG (1) | BG60254B1 (de) |
| CA (1) | CA1284119C (de) |
| DE (1) | DE3585194D1 (de) |
| DK (1) | DK173054B1 (de) |
| FI (1) | FI89184C (de) |
| HU (1) | HU202581B (de) |
| IE (1) | IE59420B1 (de) |
| IL (1) | IL75584A (de) |
| NO (1) | NO175215C (de) |
| PH (1) | PH21679A (de) |
| PL (1) | PL152355B1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL80529A0 (en) * | 1985-11-14 | 1987-02-27 | Daiichi Seiyaku Co | Method of producing peptides |
| EP0228036A3 (de) * | 1985-12-18 | 1988-09-07 | Microgenesys, Inc. | Verfahren zur Herstellung von spezifischen Polypeptiden in virusinfizierten Insektzellen und daraus isolierte Polypeptide |
| US5071748A (en) * | 1986-09-09 | 1991-12-10 | Genetics Institute, Inc. | Mixed baculovirus compositions and uses thereof |
| JPS6474990A (en) * | 1987-09-17 | 1989-03-20 | Susumu Maeda | Vector, recombinant dna, virus and production of protein |
| US5145775A (en) * | 1989-02-28 | 1992-09-08 | Research Association For Biotechnology Of Agricultural Chemicals | Polyhedrin gene and genetic engineering thereof |
| CA2019803C (en) * | 1989-06-29 | 2000-04-25 | Akira Yanai | Feline interferon and process for production thereof |
| JP2525054B2 (ja) * | 1989-08-01 | 1996-08-14 | 株式会社トクヤマ | 成人ｔ細胞白血病ウィルス感染診断薬 |
| EP0533469A1 (de) * | 1991-09-18 | 1993-03-24 | Hong Kong Tech Company Limited | Expressionsvektor und diesen enthaltender Seidenwurmtransformant |
| JPH05227967A (ja) * | 1992-02-17 | 1993-09-07 | Katakura Kogyo Kk | カイコからの有用タンパク質の製造方法 |
| US6575013B2 (en) * | 2001-02-26 | 2003-06-10 | Lucent Technologies Inc. | Electronic odor sensor |
| AU2004221609B2 (en) | 2003-03-18 | 2008-10-23 | Air Products And Chemicals, Inc. | Integrated multiple-loop refrigeration process for gas liquefaction |
| WO2007135953A1 (ja) | 2006-05-19 | 2007-11-29 | Yamaguchi University | チョウ目昆虫用人工飼料及びその製造方法、チョウ目昆虫及びその製造方法、並びに生体物質 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0127839B1 (de) * | 1983-05-27 | 1992-07-15 | THE TEXAS A&M UNIVERSITY SYSTEM | Verfahren zur Herstellung eines rekombinanten Baculovirus-Expressionsvektors |
| US4745051A (en) * | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
| ZA848495B (en) * | 1984-01-31 | 1985-09-25 | Idaho Res Found | Production of polypeptides in insect cells |
-
1984
- 1984-06-21 JP JP59128215A patent/JPS619288A/ja active Granted
-
1985
- 1985-06-11 FI FI852322A patent/FI89184C/fi not_active IP Right Cessation
- 1985-06-17 NO NO852433A patent/NO175215C/no unknown
- 1985-06-19 PH PH32421A patent/PH21679A/en unknown
- 1985-06-20 PL PL1985254100A patent/PL152355B1/pl unknown
- 1985-06-20 IL IL7558485A patent/IL75584A/en not_active IP Right Cessation
- 1985-06-20 HU HU852434A patent/HU202581B/hu unknown
- 1985-06-21 AT AT85107711T patent/ATE71655T1/de not_active IP Right Cessation
- 1985-06-21 DE DE8585107711T patent/DE3585194D1/de not_active Expired - Lifetime
- 1985-06-21 CA CA000484915A patent/CA1284119C/en not_active Expired - Lifetime
- 1985-06-21 IE IE154885A patent/IE59420B1/en not_active IP Right Cessation
- 1985-06-21 KR KR1019850004412A patent/KR930000273B1/ko not_active IP Right Cessation
- 1985-06-21 AU AU43943/85A patent/AU588236B2/en not_active Expired
- 1985-06-21 BG BG070778A patent/BG60254B1/bg unknown
- 1985-06-21 EP EP85107711A patent/EP0175852B1/de not_active Expired - Lifetime
- 1985-06-21 DK DK198502823A patent/DK173054B1/da not_active IP Right Cessation
-
1991
- 1991-04-12 US US07/684,486 patent/US5118616A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| BG60254B2 (en) | 1994-03-24 |
| DK173054B1 (da) | 1999-12-06 |
| JPH0573389B2 (de) | 1993-10-14 |
| IL75584A (en) | 1994-05-30 |
| PH21679A (en) | 1988-01-13 |
| DK282385D0 (da) | 1985-06-21 |
| NO852433L (no) | 1985-12-23 |
| EP0175852A3 (en) | 1987-09-16 |
| PL152355B1 (en) | 1990-12-31 |
| AU4394385A (en) | 1986-01-02 |
| DE3585194D1 (de) | 1992-02-27 |
| IE851548L (en) | 1985-12-21 |
| NO175215C (no) | 1994-09-14 |
| KR930000273B1 (ko) | 1993-01-14 |
| HUT38969A (en) | 1986-07-28 |
| KR860000383A (ko) | 1986-01-28 |
| PL254100A1 (en) | 1986-08-12 |
| FI852322A0 (fi) | 1985-06-11 |
| EP0175852B1 (de) | 1992-01-15 |
| HU202581B (en) | 1991-03-28 |
| IL75584A0 (en) | 1985-10-31 |
| AU588236B2 (en) | 1989-09-14 |
| FI89184C (fi) | 1993-08-25 |
| EP0175852A2 (de) | 1986-04-02 |
| DK282385A (da) | 1985-12-22 |
| CA1284119C (en) | 1991-05-14 |
| FI852322L (fi) | 1985-12-22 |
| JPS619288A (ja) | 1986-01-16 |
| BG60254B1 (bg) | 1994-03-24 |
| ATE71655T1 (de) | 1992-02-15 |
| FI89184B (fi) | 1993-05-14 |
| IE59420B1 (en) | 1994-02-23 |
| US5118616A (en) | 1992-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chisholm et al. | Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus IE-1 gene | |
| US5194376A (en) | Baculovirus expression system capable of producing foreign gene proteins at high levels | |
| DK170236B1 (da) | Rekombinante DNA-molekyler, fremgangsmåde til fremstilling af et polypeptid af IFN-alfa typen og fremgangsmåde til udvælgelse af DNA-sekvenser, der koder for polypeptider af IFN-alfa typen | |
| EP0429483B1 (de) | Potyvirus-hüllproteingene und damit transformierte pflanzen | |
| NO173944B (no) | Fremgangsmaate for fremstilling av en rekombinant baculovirus-ekspresjonsvektor | |
| US5229293A (en) | Recombinant baculovirus | |
| WO1990010078A1 (en) | Improved baculovirus expression system capable of producing foreign gene proteins at high levels | |
| NO165201B (no) | Fremgangsmaate for fremstilling av hybrid munamleukocyttinterferoner paa 165-166 aminosyrer omfattende en subsekvens fra human leukocytt-interferon a og en subsekvens fra human leukocytt-interferon b eller d. | |
| NO175215B (de) | ||
| NO177540B (no) | Fremgangsmåte for fremstilling av heterologe proteiner i Bombyx mori samt plasmid og rekombinant Bombyx mori nukleært polyhedrosevirus (BmNPV) | |
| EP0118393A2 (de) | Methoden und Zusammensetzungen für die Expression entsprechender eucaryotischer Gen-Produkte | |
| KR970002670B1 (ko) | 고양이 인터페론 및 이의 제조방법 | |
| JPH0365191A (ja) | スフェロイジン単離ｄｎａおよび組み換え昆虫ポックスウィルス発現ベクター | |
| Gauss et al. | The bacteriophage T4 dexA gene: sequence and analysis of a gene conditionally required for DNA replication | |
| EP0222412B1 (de) | Verfahren zur Herstellung von Peptiden | |
| FR2481316A1 (fr) | Technique a adn de recombinant pour la preparation d'une proteine ressemblant a l'interferon humain | |
| CN108384763B (zh) | 一种传染性脾肾坏死病毒orf074基因缺失株及其制备方法和应用 | |
| CN108048479B (zh) | 一种利用里氏木霉作为宿主表达重组蛋白的方法 | |
| CN108384764B (zh) | 一种传染性脾肾坏死病毒orf069基因缺失株及其制备方法和应用 | |
| US5003058A (en) | DNA coding for antigen protein of rinderpest virus | |
| CN108220252B (zh) | 一种传染性脾肾坏死病毒orf022基因缺失株及其制备方法和应用 | |
| CA1312027C (en) | Methods and compositions for expression of competent eukaryotic gene products | |
| CN1016796B (zh) | 用重组的中国家蚕核多角体病毒制备异源基因产物的方法 | |
| GB2228486A (en) | Improved baculovirus expression system capable of producing foreign gene proteins at high levels | |
| JPS619297A (ja) | ペプチド類の製法 |