JPS6474990A - Vector, recombinant dna, virus and production of protein - Google Patents

Vector, recombinant dna, virus and production of protein

Info

Publication number
JPS6474990A
JPS6474990A JP62231107A JP23110787A JPS6474990A JP S6474990 A JPS6474990 A JP S6474990A JP 62231107 A JP62231107 A JP 62231107A JP 23110787 A JP23110787 A JP 23110787A JP S6474990 A JPS6474990 A JP S6474990A
Authority
JP
Japan
Prior art keywords
gene
nuclear
protein
virus
recombinant dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62231107A
Other languages
Japanese (ja)
Inventor
Susumu Maeda
Nobuyuki Kobayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP62231107A priority Critical patent/JPS6474990A/en
Publication of JPS6474990A publication Critical patent/JPS6474990A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE:To mass produce HIV antigen protein, by integrating a manifestation vector of DNA of nuclear polyhedroisis virus of silkworm with a gene of HIV antigen active protein and using the prepared recombinant DNA. CONSTITUTION:A gene of gag, pol, SOR gp120 or gp41 of HIV is integrated into a polylinker range of transfer vector pBFs containing a promoter range of a polyhedron protein structure gene of DNA of nuclear polyhedroisis virus silkworm to construct recombinant DNA. A recombinant nuclear polyhedroisis virus infected with the recombinant DNA is multiplied nuclear in a culture cell or host to give a large amount of HIV antigen protein. Plasmid pBFs can be constructed from plasmid pBmE36 containing EcoRI-EcoRI fragment of polyhedron gene of silkworm, contains ATG code, a signal of a protein synthesis initiator of nuclear polyhedron gene and has a polylinker and a termination codon TAA of polyhedron gene after the ATG code.
JP62231107A 1987-09-17 1987-09-17 Vector, recombinant dna, virus and production of protein Pending JPS6474990A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62231107A JPS6474990A (en) 1987-09-17 1987-09-17 Vector, recombinant dna, virus and production of protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62231107A JPS6474990A (en) 1987-09-17 1987-09-17 Vector, recombinant dna, virus and production of protein

Publications (1)

Publication Number Publication Date
JPS6474990A true JPS6474990A (en) 1989-03-20

Family

ID=16918409

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62231107A Pending JPS6474990A (en) 1987-09-17 1987-09-17 Vector, recombinant dna, virus and production of protein

Country Status (1)

Country Link
JP (1) JPS6474990A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS619288A (en) * 1984-06-21 1986-01-16 Dai Ichi Seiyaku Co Ltd Preparation of peptide
JPS61173782A (en) * 1984-10-31 1986-08-05 カイロン・コーポレイション Recombinant virus protein relating to lymph node disease syndrome and/or acquired immunodeficient syndrome

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS619288A (en) * 1984-06-21 1986-01-16 Dai Ichi Seiyaku Co Ltd Preparation of peptide
JPS61173782A (en) * 1984-10-31 1986-08-05 カイロン・コーポレイション Recombinant virus protein relating to lymph node disease syndrome and/or acquired immunodeficient syndrome

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