NO145846B - R stabilt oksydasereagens for paavisning av neisseria-kolonie - Google Patents
R stabilt oksydasereagens for paavisning av neisseria-kolonie Download PDFInfo
- Publication number
- NO145846B NO145846B NO764026A NO764026A NO145846B NO 145846 B NO145846 B NO 145846B NO 764026 A NO764026 A NO 764026A NO 764026 A NO764026 A NO 764026A NO 145846 B NO145846 B NO 145846B
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- Norway
- Prior art keywords
- reagent
- neisseria
- stable
- oxidase
- acid
- Prior art date
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- 239000003153 chemical reaction reagent Substances 0.000 title claims description 19
- 241000588653 Neisseria Species 0.000 title claims description 6
- 201000010099 disease Diseases 0.000 title 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title 1
- 102000004316 Oxidoreductases Human genes 0.000 claims description 17
- 108090000854 Oxidoreductases Proteins 0.000 claims description 17
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- CJAOGUFAAWZWNI-UHFFFAOYSA-N 1-n,1-n,4-n,4-n-tetramethylbenzene-1,4-diamine Chemical compound CN(C)C1=CC=C(N(C)C)C=C1 CJAOGUFAAWZWNI-UHFFFAOYSA-N 0.000 claims description 2
- BZORFPDSXLZWJF-UHFFFAOYSA-N N,N-dimethyl-1,4-phenylenediamine Chemical compound CN(C)C1=CC=C(N)C=C1 BZORFPDSXLZWJF-UHFFFAOYSA-N 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 6
- 239000002609 medium Substances 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 2
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 239000012299 nitrogen atmosphere Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 241000588650 Neisseria meningitidis Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- -1 diamine salt Chemical class 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910000042 hydrogen bromide Inorganic materials 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 238000006395 oxidase reaction Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 125000003698 tetramethyl group Chemical group [H]C([H])([H])* 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/871—Neisseria
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
Foreliggende oppfinnelse vedrorer nye vannfrie oksydasereagens-opplosninger som er spesielt nyttige ved påforing av Neisseria-kolonier, som produserer enzymet cytokromoksydase.
Det er velkjent å anvende en oksydasereagensopplosning for å bestemme tilstedeværelsen av Neisseria-kolonier, såsom Neisseria gonorrhoeae eller Neisseria meningitidis. U.S. Public Health Service har etablert kriteria for presumptiv og bekreftende identifikasjon av N. gonnorhoeae. For en presumptiv diagnose må en organisme dyrket på et selektivt medium for N. gonorrhoeae identifiseres som oksydase-positiv, gram-negativ diplococci og utvise typisk kolonimorfologi. For ytterligere å bekrefte diagnosen må sukkerfermenteringer og/eller fluorescens-antile-gemebestemmelser også utfores.
I henhold til standard-laboratorieprosedyrer for bestemmelse
av N. gonorrhoeae erholdes prover med en steril dott. Sidene og tuppen av dotten rulles deretter på det selektive medium.
Et typisk fast kulturmedium for anvendelse ved dyrking av
N. gonorrhoeae er beskrevet av Martin, J. E., Jr. og Lester A.
i HSMHS Health Reports 86: 30-33, 1971. Mediet blir deretter inkubert i 24 - 48 timer og inspiseret for kolonivekst. Mis-tenkte N. gonorrhoeae-kolonier underkastes en oksydaseprove for å bestemme om enzymet cytokromoksydase er dannet. En positiv oksydaseprbvereaksjon gjenkjennes ved utvikling av en mork purpurfarge i lopet av 5 - 30 s. Hvis en omhyggelig underso-kelse av mediet ikke viser noen vekst, blir oksydasereagenset påfbrt hele mediet. Dette vil gjore det lettere å se orsmå oksydasepositive kolonier som ellers ikke ville oppdages.
Vekst av oksydasepositive kolonier gir en sikker forbestemmelse på N. gonorrhoeae. For positiv identifisering blir ubehandlede kolonier på mediet eller en annen ubehandlet kultur underkastet ytterligere bekreftende laboratorieundersøkelser, eksempelvis sukkerfermentering og fluorescens-antilegemebestemmelse.
Denkjente og kommersielt tilgjengelige oksydasereagens anvendt for å bestemme tilstedeværelsen av Neisseria-kolonier består av en 1%'ig vandig opplesning av N,N,N<1>,N'-tetrametyl-p-fenylen-diamin-dihydroklorid. Denne opplesning er imidlertid meget u-stabil. Den har en lyseblå farge når den er nyfremstilt, men går over til eh sort farge i lopet av 2 - 3 dager og er da ikke lenger effektiv med hensyn til bestemmelse av oksydase p.g.a. den raske nedbrytning av diaminsaltet. Av denne grunn er det anbefalt at den kommersielle reagensopplbsning fremstilles daglig.
P.g.a. denkjente oksydasereagensens ustabile natur er det også anbefalt at den holdes under en nitrogenatmosfære og i en her-metisk forseglet glassampulle, hvis den ikke skal anvendes umid-delbart. Ytterligere vil reagensens naturlige blåfarge repre-sentere en stadig konflikt med hensyn til den dype purpurfarge som erholdes når reagensen påfbres Neisseria-organismene og vil derfor kunne virke forstyrrende på bestemmelsen.
Det er derfor en hensikt med foreliggende oppfinnelse å fremstille en fargelbs, stabil oksydasreagensopplbsning for påvisning av mikroorganisme-kolonier, hvilken opplbsning kan lagres relativt lenge.
Med den nye oksydasereagenspreparat ifølge foreliggende oppfinnelse unngås overraskende stabilitetsproblemer, som det kjente oksydasereagens er beheftet med.
Oksydasereagensopplbsningen ifblge oppfinnelsen er fargelbs
og stabil. F.eks. forblir reagensopplbsningen ifblge oppfinnelsen fargelbs opptil 8 uker og bibeholder en utmerket aktivitet. Opplesningen kan inneholdes i dryppeflasker og be-hovet for pakking under nitrogenatmosfære er eliminert.
Ytterligere, den stabile vannfrie oksydasereagensopplosning ifblge oppfinnelsen gir en meget bedre kontrast, mellom koloniene og mediet. Anvendelsen av en fargelbs opplbsning som utgangsmate-riale forenkler vesentlig observasjonen og tolkingen av koloniene , som får en dyp purpurfarge i nærvær av reagensopplbsningen.
Den stabile vannfrie oksydasereagensopplosning ifblge foreliggende oppfinnelse omfatter tetrametyl- eller dimetyl-p-fenylen-diamin i dimetylsulfoksyd. Fenylendiaminet vil utgjore 0,1 -
1,0 vekt-% av blandingen. Fortrinnsvis er fenylendiaminet tilstede i en mengde på 0,2 - 0,5 vekt-%. Mest fordelaktig vil den stabile reagensopplbsning ifblge oppfinnelsen inneholde N,N,N' , N'-tetrametyl-p-fenylendiamin.
Oppfinnelsen innbefatter også ikke-toksiske, farmasbytisk akseptable addisjonssalter av de ovenfor definerte baser erholdt med organiske eller uorganiske syrer. Slike salter lar seg lett fremstille ved kjent teknikk. Basen omsettes enten med den stbkiometriske mengde av organisk eller uorganisk syre i et med vann blandbart opplbsningsmiddel, såsom aceton eller etanol, med isolasjon av saltet ved inndampning og avkjbling eller med et overskudd av syren i et med vann ublandbart opplbsningsmiddel , såsom etyleter eller kloroform, hvorved det bns-
kede salt faller ut direkte. Eksempler på slike organiske salter er de som erholdes med maleinsyre, fumarsyre, benzosyre, ravsyre, eddiksyre, propionsyre, vinsyre, salicylsyre, sitron-syre, stearinsyre og palmitinsyr e. Eksempler på slike uorganiske salter er de som erholdes med saltsyre, bromhydrogen-
syre, svovelsyre, fosforsyre og salpetersyre. Disse salter kan også fremstilles ved den klassiske metode ved dobbeltspalt-ning av passende salter, slik som er velkjent innen teknikkens stand.
De fblgende eksempler illustrerer oppfinnelsen.
Eksempel 1
Den frie base opploses i dimetylsulfoksyd til å gi en klar, fargelbs opplbsning.
Eksempel 2
Dihydrokloridsaltet opploses i dimetylsulofksyd til å gi en klar, fargelos opplbsning.
Eksempel 3
Basen opploses i dimetylsulfoksyd.
Claims (1)
- Stabil oksydasereagensoppløsning for påvisning av Neisseria-kolonier omfattende 0,1 - 1,0 vekt%. N,N,N',N'-tetrametyl-p-fenylendiamin eller N,N-dimetyl-p-fenylendi-amin eller syreaddisjonssalter derav, karakterisert ved at som oppløsningsmiddel er anvendt dimetylsulfoksyd.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/643,465 US4017420A (en) | 1975-12-22 | 1975-12-22 | Stable oxidase reagent solutions |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO764026L NO764026L (no) | 1977-06-23 |
| NO145846B true NO145846B (no) | 1982-03-01 |
| NO145846C NO145846C (no) | 1982-06-09 |
Family
ID=24580942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO764026A NO145846C (no) | 1975-12-22 | 1976-11-25 | Stabilt oksydasereagens for paavisning av neisseria-kolonier |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US4017420A (no) |
| JP (1) | JPS5915636B2 (no) |
| AT (1) | AT350734B (no) |
| AU (1) | AU507640B2 (no) |
| BE (1) | BE848697A (no) |
| BR (1) | BR7608015A (no) |
| CA (1) | CA1069030A (no) |
| CH (1) | CH624144A5 (no) |
| DE (1) | DE2653821C2 (no) |
| ES (1) | ES453758A1 (no) |
| FI (1) | FI55521C (no) |
| FR (1) | FR2336481A1 (no) |
| GB (1) | GB1565675A (no) |
| IT (1) | IT1064494B (no) |
| LU (1) | LU76437A1 (no) |
| NL (1) | NL7613092A (no) |
| NO (1) | NO145846C (no) |
| SE (1) | SE422080B (no) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4372874A (en) * | 1976-09-13 | 1983-02-08 | Modrovich Ivan Endre | Stabilization of hydrolysis prone labile organic reagents in liquid media |
| DE2716060C3 (de) * | 1977-04-09 | 1980-06-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisierte Schnelldiagnostica mit Oxydationsindikatoren |
| DE3114935C2 (de) * | 1981-04-13 | 1983-12-22 | Schöbel, Barbara, Dr., 6220 Rüdesheim | Verfahren zur Bestimmung von Wasserstoffperoxid |
| EP0125698B1 (en) * | 1983-05-16 | 1989-12-13 | NABISCO BRANDS, Inc. | A process for screening microorganisms producing glucose-2-oxidase |
| US7909895B2 (en) * | 2004-11-10 | 2011-03-22 | Enertech Environmental, Inc. | Slurry dewatering and conversion of biosolids to a renewable fuel |
| EP1894893B1 (en) * | 2005-04-27 | 2014-09-24 | Mitsubishi Kakoki Kaisha, Ltd | Organic waste disposal facility and method of disposal |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2970945A (en) * | 1958-10-03 | 1961-02-07 | Miles Lab | Diagnostic composition |
| US3876503A (en) * | 1971-10-29 | 1975-04-08 | Frederick C Mennen | Method and instrument for the detection of neisseria gonorrheae without culture |
-
1975
- 1975-12-22 US US05/643,465 patent/US4017420A/en not_active Expired - Lifetime
-
1976
- 1976-11-16 GB GB47720/76A patent/GB1565675A/en not_active Expired
- 1976-11-22 SE SE7613025A patent/SE422080B/xx not_active IP Right Cessation
- 1976-11-23 CA CA266,344A patent/CA1069030A/en not_active Expired
- 1976-11-23 AU AU19919/76A patent/AU507640B2/en not_active Expired
- 1976-11-24 JP JP51141752A patent/JPS5915636B2/ja not_active Expired
- 1976-11-24 BE BE172654A patent/BE848697A/xx not_active IP Right Cessation
- 1976-11-24 FI FI763377A patent/FI55521C/fi not_active IP Right Cessation
- 1976-11-24 NL NL7613092A patent/NL7613092A/xx not_active Application Discontinuation
- 1976-11-25 IT IT29779/76A patent/IT1064494B/it active
- 1976-11-25 NO NO764026A patent/NO145846C/no unknown
- 1976-11-25 AT AT875876A patent/AT350734B/de not_active IP Right Cessation
- 1976-11-26 CH CH1493676A patent/CH624144A5/de not_active IP Right Cessation
- 1976-11-26 DE DE2653821A patent/DE2653821C2/de not_active Expired
- 1976-11-29 FR FR7635929A patent/FR2336481A1/fr active Granted
- 1976-11-29 ES ES453758A patent/ES453758A1/es not_active Expired
- 1976-11-30 BR BR7608015A patent/BR7608015A/pt unknown
- 1976-12-20 LU LU76437A patent/LU76437A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NL7613092A (nl) | 1977-06-24 |
| SE422080B (sv) | 1982-02-15 |
| ES453758A1 (es) | 1977-11-01 |
| ATA875876A (de) | 1978-11-15 |
| LU76437A1 (no) | 1977-06-10 |
| BR7608015A (pt) | 1977-11-08 |
| JPS5915636B2 (ja) | 1984-04-10 |
| SE7613025L (sv) | 1977-06-23 |
| CH624144A5 (no) | 1981-07-15 |
| FI55521C (fi) | 1979-08-10 |
| IT1064494B (it) | 1985-02-18 |
| FI763377A (no) | 1977-06-23 |
| NO764026L (no) | 1977-06-23 |
| FI55521B (fi) | 1979-04-30 |
| AT350734B (de) | 1979-06-11 |
| US4017420A (en) | 1977-04-12 |
| JPS5282777A (en) | 1977-07-11 |
| DE2653821A1 (de) | 1977-06-30 |
| CA1069030A (en) | 1980-01-01 |
| FR2336481B1 (no) | 1979-03-09 |
| NO145846C (no) | 1982-06-09 |
| GB1565675A (en) | 1980-04-23 |
| AU507640B2 (en) | 1980-02-21 |
| FR2336481A1 (fr) | 1977-07-22 |
| DE2653821C2 (de) | 1986-04-17 |
| AU1991976A (en) | 1978-06-01 |
| BE848697A (fr) | 1977-05-24 |
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