NL8601112A - METHOD FOR CULTIVATING PROCARYOTS AND EUCARYOTES AND USE OF THE CELLS SO PREPARED IN COSMETIC FOOD INDUSTRY AND ADDITIONAL POWDER MIXTURES. - Google Patents

Description

NL / 33.512-Kp / f. ^ Ψ

Method for the cultivation of procaryotes and eucaryotes and use of the cells thus prepared in the cosmetic food industry and additional powder mixtures.

The invention relates to a method for the cultivation of seaweed with improved biological properties, as well as its use for the cosmetic and food industry and for additional feed mixtures.

Seaweed has been used by humans for a long time. The use of seaweed for the cometic and food industry as well as for feed has also found general application.

Despite the lack of a well-developed and economically sound breeding method, seaweed species are widely used for the above purposes, although their practical application is limited due to a high fiber content. This drawback is exacerbated by the fact that the pollution of the seas in the. In recent years, there has been an increase in pollution of radioactive and carcinogenic materials, toxic heavy metals and industrial waste, which are stored in the seaweed organism. The seaweed thus obtained cannot therefore be used for cosmetic purposes as well as for the food industry and for food. For such purposes, only seaweed purified from the toxic and ballast materials can be used. One such purification method is described by Carames de 25 Gouvea in: Cosmetics and Toiletries, Vol. 95, pp. 47-50 (1980).

The purification, however, involves an action of removing some of the biologically active substances from the seaweed, with the result that the fraction of seaweed thus prepared is less valuable for cosmetic and nutritional purposes (Jochum: Die Thalassotherapy-Algologie, Kosmetic International 1982 , pp. 76-78; Zajic; Properties and Products of Algae NY, Plenum 1970.

The work-up is carried out in such a way that agar, carrageenin and alginic acid are separated from the seaweed and subsequently used separately.

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As a result of the purification methods, the valuable seaweed provitamins, for example beta-carotene, unsaturated fats and oils, such as arachine and linoleic acid, are cracked in such a way that the cosmetic preparations and the industrially obtained food mixtures as well as feed supplements with such seaweed are reduced. have activity. Seaweed, which lives or is grown on the surface of fresh water, can also store the bacteria and algae in its organism, making their application generally unsuitable for cosmetic and food purposes.

The invention relates to a method for the cultivation of seaweed, wherein on the one hand the above-mentioned drawbacks can be overcome and on the other hand the seaweed thus prepared has a significantly higher activity than the known seaweeds.

It has been found that by cultivating seaweed strains in a culture medium containing sterilized mineral, medicinal or thermal water of natural origin or mixtures thereof, seaweeds are obtained, whereby the storage of toxic substances (mercury, lead, cadmium, radioactive pollution , bacteria, viruses, carcinogen and the like) are excluded.

It has been observed that suitable substances, such as microelements and vitamins, which are present in medicinal waters, have been incorporated into the organism of the seaweeds prepared according to the invention.

The medicinal use of such substances has proved to be very important for several centuries.

The active ingredients and microelements from the medicinal waters are stored in the seaweeds prepared according to the present method, while on the other hand these substances form addition and complex compounds with the mucoproteins present in the seaweed.

It is not necessary to subject the seaweeds obtained by the present method to any subsequent purification or heat treatment. This means that the seaweed as a whole can be mixed with various cosmetic or industrial food preparations as well as with feed additives. This means that the active ingredients from seaweeds are not damaged, with the result that the full biological value is preserved. The other and surprising advantage of the seaweeds thus prepared consists in that the micro-organisms indispensable for the microorganism and for the skin are introduced into an organism in a worm bound to the mucoprotein proteins. The microelements thus introduced are absorbed and exert a higher activity in the human or animal organism.

The cosmetic preparations containing the algae thus prepared are suitable not only for increasing the water content of the skin, but also for optimizing the action of the enzymes of organisms by introducing the microelements, which are readily absorbed. bare form.

This also applies to the vitamins and oils, which are introduced together with the seaweeds. A similar effect also manifests itself in the case of industrial food mixtures and feed mixtures.

The cometic compositions of the invention promote the hydration of the water-deficient skin and restore cells of the aged and wrinkled skin. When used as hair conditioners or toothpastes, a "roborating" action occurs after absorption, which has a beneficial effect on the development of their roots. The loss of teeth is also prevented.

30 All these excellent and unexpected effects are due to the fact that the organism indispensable substances can be introduced into the human organism in a form bound to the proteins of seaweed organisms, thus increasing the extent of utilization (bioavailability). is greatly increased.

Due to the introduction of the microelements into the organism as such, they are often not converted into a biologically active form, with the result that they cannot participate in the life process.

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For example, zinc is a component of at least thirty enzymes from organisms and therefore plays an important role in skin protein synthesis. Zinc-containing enzymes participate in the synthesis of DNA and RNA.

The information transfer required for the synthesis of proteins is hindered by the absence of these zinc-containing enzymes, making it difficult to repair the skin.

In case of. copper deficiency damages the colla-10 gene structure of the skin.

The function of the hematopotetic organs is hampered by an iron deficiency, which is aggravated by the fact that iron compounds, introduced as such, are insufficiently absorbed into the organism. Using the present seaweeds, the micro-element deficiency in the organism can be greatly reduced.

A particularly beneficial effect can be obtained by cultivating the seaweeds in such a culture medium according to the invention, in which the metal content is enriched by adding an additional metal compound. It is convenient to adjust the metal ion content of the culture medium to a concentration of up to 10 mol / liter with respect to each metal ion. Various kinds of seaweed strains can be grown in the same culture medium at a temperature between 0 ° C and 80 ° C. Then sterile airflow with carbon dioxide therein is bubbled into the inoculated culture medium. The carbon dioxide concentration of the air is 0.5 - 20% by volume, preferably 1.5 - 5%. During the bubbling in of air it is advantageous to illuminate the culture medium by means of a fluorescent lamp.

A cultivation period lasts 7-30 days, preferably 12-14 days. The seaweeds obtained are separated from the culture medium and then generated. The work-up can be carried out using known physical or chemical methods. The purpose of this work-up is to break a site of combination by a mechanical method or by changing the osmotic pressure, so as to release the cell contents.

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Work-up can take place as follows: - by heat treatment to 40-200 ° C under a pressure of 0-60 bar (0 - 5000 kPa), * - by ultrasonic treatment; 5 - by treatment with a surfactant (surfactant), such as sodium lauryl sulfate or - by a mechanical method, such as, for example, by grinding in the presence of a solid support.

The reprocessed seaweeds 10 treated in this way can optionally be further reacted with the solution of a metal compound, in order to enrich a metal ion concentration by at least 10 mol / kg per solid seaweeds. The seaweeds thus obtained can be mixed with the cosmetic preparation after drying or without drying in the form of an aqueous suspension.

The cosmetic preparation, as well as the industrial food and feed additive, contains the seaweeds in an amount of 0.05 - 95% by weight, based on the dry matter content, mixed with known liquid or solid additives.

Particularly favorable mixtures can be achieved by adding an active additive in an amount of 0.05 - 50% to the preparations. This active additive may be virgin wax, pollen or other vegetable powder in addition to globin, protein, an animal protein, a plasma protein and the like.

It is preferable to add the preparations as additives oils, fats of vegetable or animal origin, surfactants, starch (fecula) of vegetable origin, bran as well as preservatives, solid liquid carriers.

In summary, the invention includes a method of growing seaweeds with better biological properties.

The method according to the invention is characterized in that a culture medium of medicinal water, mineral water, thermal water of natural origin or mixtures thereof is sterilized, optionally enriched with metal compounds to a concentration of 10 40 mol / liter, based on each metal ion, at a pressure of -2 -6- at most 10 bar (1000 kPa) and a temperature of 100 ° C-140 ° C followed by inoculation with a pure seaweed culture, after which a sterile air flow with 0.5 -20 volume percent in the culture medium carbon dioxide is bubbled into it at room temperature, followed by isolation of the seaweeds after a culture period of 7-30 days at 0 ° - 80 ° C, followed by working up the seaweeds thus obtained in known physical or chemical manner, the seaweeds thus processed are optionally reacted with metal compounds in order to obtain an enriched concentration of up to 10 mol / kg, based on each metal ion.

The invention furthermore relates to cosmetic preparations containing the seaweeds 15 thus prepared in an amount of 0.05 - 95% by weight, based on the dry matter and optionally 0.05 - 50% by weight, of active additives, preferably virgin wax, globin, protein, dry vegetable powder as well as known cosmetic additives, preferably oils and fats of vegetable or animal origin, surfactants, preservatives, liquid or solid diluents in an amount of up to 100% by weight.

The cosmetic preparations thus prepared can be used as skin treatment agents for moisturizing and restoring the skin, for increasing muscle tone and for wrinkle-freeing of aged skin. The hair treatment compositions according to the invention are suitable for stimulating the hair roots, for nourishing the skull, while the dental treatment compositions according to the invention can be used to alleviate gum atrophy as well as to prevent the loosening of teeth and dental caries .

In addition, the invention relates to industrial food mixtures and feed additives, containing the seaweeds prepared according to the invention in an amount of 0.05 - 95% by weight, based on the dry matter and in addition possibly active additives, preferably honey, blood plasma, globin, corn germ and 40 dry vegetable powder in an amount of 0.05 - 50 8601112 -7- «<- - <x weight percent as well as known additives commonly used in the food industry, suitable preservatives, liquid solid carriers in an amount up to at 100% by weight.

5 It. food prepared according to the present method and the additives which can be mixed with feed have a "roborating" effect on the human and animal organism and promote an optimal action of the enzyme system of the organism. Any deficiency of the vitamins or microelements in the human or animal organism is additionally supplemented by use of the present compositions.

A regenerating effect on the damaged and aged organism is also obtained with the present compositions.

The method and preparations according to the invention are further illustrated by the following non-limiting examples.

Example I.

20 Growing seaweeds.

Seven liters of a medicinal water from the Lukacs Bad from Budapest are transferred to a seaweed culture flask and sterilized at 121 ° C. pressure of 1 bar (100 kPa) for 30 minutes.

The sterile solution was inoculated using pure culture of Scenedesmus obtisiusculus, after which sterile air with 1.5% by volume carbon dioxide was bubbled into the culture medium at 25 ° C under illumination with a luminescent lamp. After a 14 day culture period, seaweeds were isolated from the culture medium. The seaweeds thus obtained were found to contain the valuable microelements of the medicinal water without any toxic material in them.

The seaweeds were worked up by a sound wave treatment, after which the aqueous material thus obtained was used for cosmetic preparations.

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-8- ^ example_ | I.

Seaweed cultivation.

The procedure described in Example I was repeated except that a medicated water from Heviz was used.

Example_III_.

Seaweed cultivation.

The procedure described in Example I was repeated, except that a medicinal water from Hajduszoboszló was used and no treatment with sound waves took place.

Yes ^ IV ^

Seaweed cultivation.

The procedure 15 described in Example I was repeated, except that as medicinal water, water from Piestrany (Czechoslovakia) was used, while after seeding with the culture a stable air stream was bubbled into the culture medium containing 5% by volume of carbon dioxide, in which the bubbling took place at 25 ° C under 20 exposure with a fluorescent lamp.

Example_V-1__

Seaweed cultivation.

The procedure described in Example I was repeated "6, except that 10 g / ml gold (III) chloride was added to the medicinal water in sterile manner.

Example_VI_1 Cultivation of seaweeds.

The procedure of Example 1 was repeated, except that 10 g / ml iron (III) chloride was added to the medicated water in a sterile manner.

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Seaweed cultivation.

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The procedure of Example II was repeated except that 10 mg / liter of lithium fluoride was added to the medicinal water-based culture medium.

Example VIII.

5 Seaweed farming.

The procedure described in Example I was repeated, except that mineral water from Parad (Hungary) supplemented with 1 g / liter of potassium nitrate of 0.2 g / liter of dipotassium hydrogen phosphate was used instead of the midicinal water. The pH of the culture medium was adjusted to 6.5 by adding 2 N hydrochloric acid solution.

Work-up was carried out chemically instead of sonication by mixing 4 g of seaweeds with 825 g of water and 392 g of sodium lauryl ether sulfate, followed by heating the solution to 60 ° C.

Example IX.

Seaweed cultivation.

The procedure described in Example I was repeated, except that Chlorella pyrenoi-20 dosa was used as a seaweed stock instead of Scenedesmus obtisiusculus.

Example X.

. Preparation of face and body treatment preparations.

25 of the seaweeds grown in Example I were suspended in 412 g of water at 70 ° C, after which it was mixed at 80 ° C with a melt of 70 g of white petroleum jelly, 70 g of stearin monogylceride, 82 g of cetyl alcohol, 27 g of corn germ oil and 27 g Tween 60 sorbitan ester polyoxyethylene derivatives. The mixture was then cooled and stirred under stirring by addition of 0.2% Kathon CG.

Using the composition thus obtained as a face and body treatment composition, excellent hydration of the skin regeneration activity can be obtained.

Example XI.

Preparation of shampoo.

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184 g of Alfozid KT-25, 152 g of Zala betaine 35%, 20 g of Alfid D 11 and 0.2% Kathon CG preservative were added to the 4 g seaweed mixture prepared according to Example VIII be-5. wrote way. The product thus obtained was used as a shampoo for hair.

Regular use of this shampoo showed that hair loss was less, while fragile hair started to shine and moreover the hair appeared to be flexible.

Example XII.

Preparation of a preparation suitable for iontophoresis.

1.4 ml of the seaweed suspension prepared according to Example VIII was weighed into a 2 ml ampoule, after which 0.5 g of an alcoholic solution of 3% by weight of stomach wash was added. After closure, the ampules were sterilized.

The preparation thus obtained can be used for regenerating old and wrinkled skin, as well as for an ionophoretic treatment to make the skin supple.

Example XIII,

Preparation of a hydration cream.

Of the seaweeds grown and raised in accordance with Fig. 2, 4g was mixed with 20 ml of an alcoholic solution of 2% by weight of virgin wax, and the method described in Example X was followed, yielding an excellent skin and body treatment composition, which makes the skin supple after regular application.

Example XIV.

Preparation of a skin-regenerating face and body treatment preparation.

Four g of the seaweed grown according to Example III is heated to 80 ° C in the presence of 1% sodium hydrogen carbonate with 100 ml of a solution of 5% by weight globin prepared from pig blood, * -11- of which the procedure described in Example X is followed after delivery of an excellent skin and body treatment preparation, which makes the skin supple with regular use.

Example XV.

Preparation of a skin regenerating body treatment composition.

The procedure described in Example XIV was followed with the proviso that instead of the globin solution, 100 ml of water a suspension of 5 g of zernet powder and 1% sodium hydrogen carbonate was used to yield a skin-repairing body treatment composition.

Example XVI.

15 Preparation of a skin regenerating. facial and body treatment preparation.

The procedure described in Example XIV was followed, except that 25 g of quail protein was used instead of globin to yield the formulation which is particularly suitable for refreshing and moisturizing aged skin.

Example XVII.

Comparative example.

The face and body treatment composition according to Example XVI was prepared, but instead of the seaweeds grown in Example III, an unpurified seaweed of seawater original was mixed into the composition. Both preparations were tested on groups of people, each group consisting of 10 people. The preparation containing seaweed of seawater origin caused skin blemishes and inflammation of the skin after 3 days of treatment, so that the use of this preparation had to be discontinued. The skin of the subjects treated with the preparation prepared according to Example XVI became tight and wrinkle free after regular use for two weeks. The skin inflammation and spots on the skin were not detectable.

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Example XVIII.

* ·

Preparation ::. Of toothpaste.

120 g glycerol, 7 g alginate, 1 g saccharin sodium, 2 g nipagine M, 30 g polyethylene glycol and 60 g Dentolex 684, 180 g Dentolex 654 and 330 g sorbitol were mixed under pressure in an installation equipped with a stirrer, after which 200 g of a suspension of 2 weight percent seaweeds was added. Then, a mixture of 20 weight percent sodium lauryl sulfate and 80 weight percent 10 percent glycerol is added while the mixture is homogenized. The thus obtained toothpaste was imparted a fragrance by adding 1 g of menthol from the revival of toothpaste with a relieving effect on gum bleeding and paradentosis.

15 Example XIX.

Preparation of a "roborating" tablet.

909, g.b.lo.edplasmap.oeder>. 10 g seaweed> IZO-r as described in Example IV, 90 g potato starch and 900 g medicated water from the Lukacs bath of Budapest 20 were mixed and then granulated. After adding 4 g of magnesium stearate to the dry granulate, the product obtained was compressed into tablets.

Regular consumption of tablets enabled both very young and elderly persons to be "robora-25 ted".

Example XX.

Preparation of a drinking juice.

100 g of a suspension of 2 g of seaweed prepared in the manner described in Example VIII was added to 900 g of a fibrous apple juice, after which the mixture was homogenized.

Regular drinking of this juice completed any iron deficiency of the organism, while a regenerative action was performed on the entire organism.

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Example XXI.

Preparation of a honey preparation with increased biological activity.

300 g of a seaweed mixture (calculated on the dry matter) prepared in the manner described in Example II was mixed with a mixture of 600 g of honey and 100 g of apple juice. After homogenizing the mixture, a preparation was obtained which was particularly suitable for repairing weak aged and pathogenic sick organisms.

Example XXII.

Preparation of a feed mixture.

To 500 g of the seaweed suspension (based on the dry matter) in the manner described in Example III, 500 g of bran was added portionwise and the mixture was homogenized. The mixture thus prepared was used as a feed additive. The regular use of this preparation, after adding it to the feed of fur animals, showed that the quality of the fur was improved.

Claims (8)

Method for the cultivation of seaweeds with improved biological properties, characterized in that a culture medium of medicinal water, mineral water or thermal water of natural origin 5 or a mixture thereof is sterilized, optionally enriched with metal compounds to a concentration of -2 10 mol / liter, based on each metal ion, at a pressure of up to 10 bar (1000 kPa) and a temperature of 100-140 ° C, followed by inoculation with a pure seaweed culture, then 10 in the culture medium a sterile air flow containing 0 . 5-20 volume percent carbon dioxide bubbled in at room temperature, followed by isolation of the seaweeds after a culture period of 7-30 days at 0 ° -80 ° C, after which the seaweeds are optionally worked up in a manner known physically or chemically, after which the thus processed seaweeds are reacted with metal compounds until a concentratie2 concentration of at most 10 mol / kg based on each metal ion is reached. 2. A method according to claim 1, characterized in that a sterile air stream containing 1.5-5% by volume of carbon dioxide is bubbled in through the culture medium. 3. A method according to claim 1, characterized in that the seaweeds are worked up using ultrasonic waves. 4. Cosmetic preparation, preferably skin treatment, hair treatment preparation or toothpaste with the seaweed prepared according to claim 1 in an amount of 0.05 - 95% by weight, based on dry matter, optionally together with active additives in an amount of 0, 05-50% by weight, preferably stomach wash, globin, protein, dry vegetable powder as well as known cosmetic additives, preferably oils, fats of vegetable or animal origin, surfactants, preservatives, liquid or solid connecting agents in an amount up to to 100 weight percent. 5. Preparation according to claim 4, characterized in that the preparation contains 20% by weight of W w w -v-1 -15- ~ ..... globin as active filler. An additive for industrially obtained food or feed containing the seaweed prepared according to claim 1 in an amount of 0.05-95% by weight, based on dry matter, optionally together with the active additives in an amount of 0.05 -50% by weight, preferably honey, globin, blood plasma, dry vegetable powder as well as known additives, for example preservatives, liquid or solid diluents in an amount up to 100% by weight. 7. A composition according to claim 6, characterized in that the active additive composition contains 95% by weight of honey together with a water suspension of 5% by weight of seaweeds, based on the dry matter. 8. Method and preparation according to claims 1-7, wherein the fluorides and / or precious metal salts are used as additives to the culture medium and / or the preparations. 86 0: 1 1 2