HU195069B - Method for growing algae of improved biologic feature and beauty and body hygienic preparations containing the such produced algae - Google Patents

Abstract

A nutrient soln. consisting of natural, respectively carbonated, medicinal and thermal waters or their mixts., is enriched by metallic ions to raise their respective concns. to 10 power-2 moles/l., and sterilised at or below 10 bars pressure and 100-140 deg.C and inoculated with a pure culture of algae. - Carbon dioxide (0.5-2.5 vol.%) contg. sterile air is bubbled through at room temp. and the algae are removed after growing for 7-30 days ay 0-80 deg.C. Algae, in this case are chemically or polyaxially treated by known methods, and metallic cpds. are added to reach a respective ion concn. of 10 power-2 mole/kg. Cosmetic products contain 0.05-95 wt.% of treated algae based on total solids and in this case 0.05-50% active additives as propolis globin, protein, dried powder of plant origin and known cosmetic additives pref. oils of plant and animal sources, fats, surfactants, preservatives liquid or solid diluents are also added.

Description

The present invention relates to a process for the production of algae biomass and to a cosmetic and / or body care composition containing algae biomass.

The invention is carried out by medium comprising mineral water of natural origin, water, spring water or a mixture thereof - optionally metal compounds and 10 mol / liter concentration fluorides fortified fémionoként 10 ^'2 mol / liter concentration - up to 10 bars sterilized at 100-140 ° C and inoculated with algae color culture, sterile air containing 0.5-20% v / v carbon dioxide at room temperature was bubbled through the medium and after 7-30 days culture at 0-80 ° C the algae are separated and optionally the algae thus obtained are disrupted by known physical or chemical means, and then the algae is reacted with metal compounds, optionally up to a concentration of up to 10 ² mol / kg of metal ion.

The cosmetic and body care compositions of the present invention comprise from 0.05% to 95% by weight, based on the dry matter, of the algae prepared according to claim 1, optionally 0.05% to 50% by weight of active ingredient, preferably propolis, globin, protein, dried herbal powder. containing by weight the known cosmetic additives, preferably vegetable or animal oils, fats, surfactants, preservatives, liquid or solid diluents.

Algae has long been widely used by mankind. The use of algae for cosmetic purposes is widespread.

In the absence of a well-developed economical breeding process, seaweed is generally used for this purpose, although its applicability is limited due to their high fiber content. This disadvantage is exacerbated by the fact that marine pollutants accumulate in the body due to increased pollution of the seas in recent years, the presence of radioactive, carcinogenic substances, toxic heavy metals and industrial waste.

Therefore, the whole algae thus obtained cannot be used for cosmetic purposes, only the fraction purified from toxic or ballast substances. Such a purification procedure is described in M. Carames de Gouvea, Cosmetics and Toiletries, Vol. 47-50, 1980.

However, the purification operation is an intervention that destroys some of the biologically active substances in the algae, and the algae fraction thus produced is less valuable cosmetically. M. Jochum in Die Thelassotherapie-Algologie, Kosmetic International, Oct. 1982 p. 76-78. Article.

In general, the processing procedures involve the extraction of agar from algae2

-agar, carragenin, alginic acid and bovine are used separately.

Due to the purification procedures, valuable provitamins in algae, such as beta-carotene, unsaturated fats, oils such as arachinic acid, linoleic acid, are cracked and therefore cosmetic compositions containing such algae show only a reduced effect. Algae that exist or are cultivated in surface freshwater can also accumulate bacteria and viruses in their bodies, so their use in cosmetics should not be common.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an algae biomass which not only eliminates the above-mentioned disadvantages, but also has an added effect over the prior art. It is a further object of the present invention to incorporate the biologically valuable algae biomass produced in cosmetic formulations to obtain beneficial cosmetic and body care formulations.

It has been found that culturing algae strains in a sterilized medium containing natural mineral water, medicinal or thermal water, or a mixture thereof, produces algae that exclude toxic substances (mercury, lead, cadmium, radioactive impurities, bacteria, carcinogenic substances, etc.).

We have found that the algae produced by our process enrich the body with beneficial substances such as trace elements in the medicinal waters.

The use of these materials in balneology has been of great importance for centuries.

The algae produced by our method not only enrich the active substances and trace elements in the medicinal waters, but also form compounds or complex compounds with the mucoproteins in the algae.

The algae obtained according to the invention need not undergo any subsequent purification or heat treatment process; Thus, the whole algae can be mixed in various cosmetic formulations. The active ingredients in algae are not damaged and retain their full biological value. The algae produced in this way have the additional unexpected benefit of introducing trace elements, which are essential for the body and skin, to the mucoprotein protein.

The various trace elements introduced in this way can be absorbed to a greater extent and have a greater effect on the body.

Our cosmetic formulations containing algae biomasses not only enhance the water content of the skin, but also optimize the function of enzymes in the body by introducing microelements in readily absorbable form.

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The same applies to vitamins and oils taken with algae.

The cosmetic and personal care compositions of the present invention promote hydration of the dehydrated skin and regenerate the cells of the aging wrinkled skin. And when absorbed as a hair care product or toothpaste, they have a robotic effect that is beneficial to the development of hair bulbs and prevents tooth loosening.

All of this outstanding and unexpected effect can be attributed to the fact that by attaching algae-essential proteins to the human body, essential substances can be greatly improved.

When trace elements are introduced into the body by themselves, in many cases, they are not biologically active and thus cannot participate in life processes.

For example, zinc is a constituent of at least thirty enzymes in the body and thus plays a significant role in skin protein synthesis. Zinc-containing enzymes are involved in the synthesis of DNA and RNA, and their absence inhibits the transmission of information required for the production of proteins and thus impedes skin regeneration. Copper deficiency damages the collagen structure of the skin.

By using the algae according to the invention, however, the deficiency of the micro-nutrient in the organism can be greatly reduced.

A particularly beneficial effect is obtained by cultivating the algae in a nutrient medium of the invention in which the metal ion is enriched by the addition of a further metal compound. For metal ions up to 10 ² mol / liter, it is advisable to adjust the metal content of the culture medium. Not only one species but also several strains of algae can be cultured in the same medium at temperatures between 0 ° C and 80 ° C.

Sterile air containing carbon dioxide gas is then bubbled through the inoculated medium. The concentration of carbon dioxide in the air is 0.5 to 20% by volume, preferably 1.5 to 5% by volume

During bubbling, it is advisable to illuminate the medium with fluorescent tubes.

The culture cycle is 7 to 30 days, preferably

12-14 days. The algae thus obtained is separated from the culture medium and digested. The excavation may be carried out by known physical or chemical methods.

The purpose of the digestion is to break down a point in the cell membrane by mechanical means or by varying the osmotic pressure to obtain cellular contents.

The excavation may take place;

- heat treatment at 40 to 200 ° C,

At a pressure of 0 to 50 bar, - by sonication, - by treatment with a detergent, such as sodium lauryl ether sulfate, - by mechanical means, for example in the presence of a solid carrier.

The digested algae may optionally be further reacted with a solution of a sterile metal compound up to a solid algal metal ion concentration of up to 10 ^-2 mol / kg per metal ion. The resulting algae may be dried or dried in the form of an aqueous suspension in the cosmetic composition.

The compositions according to the invention contain from 0.05 to 95% by weight of algae, based on the dry matter content, with known liquid or solid additives.

Particularly advantageous formulations can be prepared by optionally incorporating 0.05-50% active ingredient into the formulations. These may be propolis, pollen or other plant powder, globin, egg protein, animal protein, plasma protein, etc.

Preferably, additional additives of vegetable or animal origin, oils, fats, surfactants, preservatives, liquid or solid carriers are incorporated into the compositions.

SUMMARY OF THE INVENTION The present invention relates to a process for the cultivation of algae with improved biological properties.

The process is characterized by sterilizing a medium containing natural mineral water, medicinal water, thermal water, or a mixture thereof, optionally enriched with metal compounds up to 10 ⁷² mol / liter per metal ion, at a temperature of 100-140 ° C at a maximum pressure of 10 bar, then inoculate with algae color culture, sterile air containing 0.5 to 20% by volume of carbon dioxide is bubbled through the culture medium at room temperature, and after 7 to 30 days of cultivation at 0 to 80 ° C, the algae are isolated by known physical or chemically digested, optionally reacting the digested algae with metal compounds to a concentration of up to 10 ³ mol / kg per metal ion.

The present invention also relates to cosmetic compositions which contain from 0.05 to 95% by weight, based on dry matter, of an algae prepared as above, optionally 0.05 to 50% by weight of an active ingredient, preferably propolis, globin, egg white, dried herbal powder, and containing by weight the known cosmetic additives, preferably vegetable or animal oils, fats, surfactants, preservatives, liquid or solid diluents.

The cosmetics thus obtained can be used as skin care products for hydrating, regenerating the skin, enhancing the muscle tone and wrinkling the aging skin. The hair care products of the present invention are intended to stimulate hair bulbs, nourish the scalp, dentifrice compositions are intended to reduce the risk of coronary heart disease, tooth decay and tooth decay.

The process and compositions of the present invention are illustrated by the following examples.

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Example 1

Growing of algae

Seven liters of Lukács bath water from Budapest are poured into an algae growing bottle and sterilized at 121 ° C for 30 minutes at a pressure of 1 bar.

The sterile solution is inoculated with Scenedesmus obtisiusculus algae culture. Sterile air containing 1.5% (v / v) carbon dioxide was bubbled through the culture medium at 25 ° C under fluorescent lighting and, after a 14-day culture cycle, the algae was separated from the culture medium. The resulting algae contains valuable trace elements of medicinal water without toxic substances.

The algae is sonicated and the water-containing material obtained is used in cosmetic formulations.

Example 2

Growing of algae

In each case, the procedure of Example 1 was followed, except that thermal water from Heviz was used.

Example 3

Growing of algae

In all cases, the procedure of Example 1 was followed, except that Hajdúszoboszló healing water was used and no ultrasonic digestion was used.

Example 4

Growing of algae

All proceed as in Example 1, with the exception that the Slovakian Piestany water is used and, after inoculation with the culture, sterile air containing 5% by volume of CO ₂ is bubbled through the culture medium using a fluorescent tube. under illumination.

Example 5

Growing of algae

All proceed as in Example 1, except that 10 mg / l of gold (III) chloride is added to the medicinal water based medium.

Example 6

Growing of algae

All proceed as in Example 2, except that 10 mg / L lithium fluoride is added to the medicinal water based medium.

Example 7

Growing of algae

All proceed as in Example 1, except that instead of the medicinal water, paraffinic mineral water was added with 1 g / l potassium nitrate and 0.2 g / l dipotassium hydrogen phosphate. The pH of the medium was adjusted to 6.5 with 2N hydrochloric acid. The digestion was not performed by sonication, but by chemical means, 4 g of algae were mixed with 825 g of water and 392 g of sodium lauryl ether sulfate. The solution was heated to 60 ° C.

Example 8

Growing of algae

All proceed as in Example 1, except that Chlorella pyrenoidosa strain was used in place of Scenedesmus obtisiusculus.

Example 9

Preparation of Facial and Body Composition Algae cultured in Example 1 g is suspended in 412 g water at 70 ° C and mixed with 70 g of white vaseline, 70 g of stearin monoglyceride, 82 g of cetyl alcohol, 27 g of corn germ oil and 27 g of Tween-60. Polyoxyethylene derivative of sorbitan ester with a melting point of 80 ° C. After mixing, the mixture was stirred until cooled and preserved with 0.2% Kathon CG.

The composition is used as a facial cosmetic. An excellent moisturizing, skin regenerating effect can be achieved.

Example 10

Preparation of Shampoo To a mixture of Example 7 containing g of algae was added 184 g of Alphoside ΚΤ-25, 152 g of 35% Zala-betaine, 20 g of Aliid D 11, and 0.2% of Kathon CG preservative. The product obtained is used as a hair care shampoo.

With regular use, hair loss is reduced, split hair becomes shiny and supple.

Example 1 Preparation for use in lontophoresis

1.4 ml of the algae suspension prepared according to Example 7 are weighed into a 2 ml ampoule. 0.5 g of an alcoholic solution containing 3% by weight of propolis are added. The vials are sealed and then sterilized.

The composition can be used for iontophoresis treatment for regeneration and elasticity of aging wrinkled skin.

Example 12

Preparation of a Hydrating Cream Algae cultured and digested in Example 2 (g) is mixed with 20 ml of an alcoholic solution containing 2% by weight of propolis and proceeded as in Example 9 below.

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You get an excellent moisturizing skin cream that is especially good for wrinkles on tired skin.

Example 13

Skin Regenerating Facial & Body Composition A 5% w / w solution of algae cultured in Example 3 in 100 ml porcine blood was heated to 80 ° C in the presence of 1% sodium bicarbonate and then proceeded as in Example 9. You get an excellent skin care, body care product that makes the skin supple when used regularly.

Example 14

Skin Regenerating Body Lotion The procedure of Example 13 was repeated except that a suspension containing 100 ml of water and 5 g of alfalfa powder in 1% w / w sodium bicarbonate was used instead of the globin solution. A skin regenerating body lotion is obtained.

Example 15

Skin regenerating face and body care product

All proceed as in Example 13, except that 25 g of quail egg protein are used instead of globin.

The product is ideal for refreshing and hydrating tired, aging skin.

Example 16

Comparative example

The face and body composition of Example 15 is prepared, but not the whole seaweed grown in Example 3, but unpurified seaweed. The composition of Example 15 and containing seaweed was tested in groups of 10-10. The preparation containing seaweed caused skin blemishes and dermatitis after 3 days of treatment and had to be discontinued. Individuals using the formulation of Example 15 became firm, wrinkle-free after two weeks of regular application. Skin inflammation, skin blemishes did not occur.

Example 17

Manufacture of toothpaste

120g glycerol, 7g alginate, 1g saccharin-Na, 2g Nipagin-M, 30g PEG M600, 60g Dentolex 684, 180g Dentolex 654, 330g sorbitol 200 g of a 2 wt% algae slurry prepared as in Example 6 are added. While homogenizing, a mixture of 60 g of 20 wt% sodium lauryl sulfate and 80 wt of glycerol is added.

The toothpaste obtained is perfumed with 1 g of menthol.

Regular use of the toothpaste thus produced reduces tooth decay and tooth decay.

Example 18

In each case, the procedure of Example 1 was followed, except that a 1: 1 mixture of Hajdúszoboszló and Hévíz healing water was used.

Example 19

In all cases, the same procedure as in Example 1 was followed, except that Paradise mineral water was used.

Example 20

In each case, we proceed as in Example 1, except that we use thermal water in Mosonmagyaróvár.

Example 21

All proceed as in Example 1 except that a 3: 1 mixture of Hajdúszoboszló Healing Water and Paradise Mineral Water is used.

Example 22

In each case, the same procedure as in Example 1 was followed, except that a 10: 1 mixture of thermal water from Mosonmagyróvár and mineral water from paradise was used.

Example 23

All proceed as in Example 1 except that the medium is sterilized at 134 ° C for 20 minutes.

Example 24

All procedures were carried out as in Example 1, except that sterile air containing 20% by volume of CO ₂ was bubbled through the culture medium at 0-1 ° C and inoculated with Nostoc commune.

Example 25

All were performed as in Example 1 except that the medium was maintained at 80 ° C and inoculated with Aphanocapsa thermalis.

Example 26

In each case, the procedure of Example 1 was followed, except that Hajdúszoboszló's medicinal water was used instead of Lukácsfürdő's water and the medium was supplemented with 1 g / l potassium nitrate, 0.2 g / l disodium hydroxide.

-5195069 with rogen phosphate and 3 g / l sodium fluoride.

Example 27

All proceed as in Example 1, except that 20 mg / L hydrogen hexachloroplatinate is added to the media.

Claims (7)

PATENT CLAIMS 1. A method to obtain biomass, characterized in that the medium containing mineral water of natural origin, medicinal water, spring water, or a mixture thereof - optionally fémiononként 10 ^'2 mol / liter concentration of metal compounds and 10 ~' fortified mol / / 1 concentration of fluorides - sterilized at a pressure of up to 10 bar at 100-140 ° C, then inoculated with algae culture, sterile air containing 0.5-20% v / v of carbon dioxide at room temperature is bubbled through the medium and then 7-30 ° C at 0-80 ° C. after days of culture, the algae are collected and optionally expose the algae was known physical or chemical means, and the detected algae fémiononként optionally reacted with up to 10 ^-2 mol / kg of concentration of metal-compounds. 2. The method of claim 1, wherein sterile air containing from 1.5 to 5% by volume of carbon dioxide is bubbled through the culture medium. 5 3. The method of claim 1, wherein the algae is exposed to ultrasound treatment. 4. A cosmetic and / or personal care composition, preferably a skin care, hair care composition or a toothpaste, characterized in that 0.05-95% by weight of the algae biomass produced according to claim 1, optionally 0.05-50% by weight of active ingredient, material, preferably propolis, globin, protein dried vegetable powder; and known cosmetic additives in an amount up to 100% by weight, preferably oils or fats of vegetable or animal origin, It contains surfactants, preservatives, liquid or solid diluents. Composition according to claim 4, characterized in that it contains 20% by weight of globin as an active filler. 25 6. A process according to any one of claims 1 to 4, wherein the noble metal salts are used as additives in the medium. 7. A composition according to any one of claims 1 to 3, characterized in that the composition contains fluorides and / or noble metal salts as additives.