DE2362673A1 - Green algae cultivated with nitrogen-fixing blue algae - in fesh water or its mixts with sea water - Google Patents

Abstract

Green algae are cultivated jointly with nitrogen-fixing blue algae or other microorganisms in fresh water or its mixts. with sea water, opt. with addn of trace elements esp. salts of Fe, Mn and Mo. The mixed algae can be used for prepn of human or animal food. The blue algae assimilate nitrogen from air, and the extracted products of their metabolism, which contain nitrogen, can be assimilated by green algae (which cannot assimilated nitrogen directly from the air). Thus, there is no need to introduce into the cultivation medium nitrates or ammonium salts or other nitrogen sources; only cpds of other nutrient elements, such as P,S, Na,K,Ca,Mg,etc and trace elements have to be supplied and only in reduced quantities if sea water is used, as it contains considerable amts of nurtitives. Thus the costs are considerably reduced, esp if the cultivation is carried out near sea-coast.

Description

Dr P. Pohl 8 Munich 90, 12/12/73.

17th

P a t e η t s c h r i f t II

"Procedure for jQ \

' together n r ^ y ^ j ^ rüiial ^ eri o_der_ ancierer ^ urgani_sriien__in common i iährl ösungeii ".

U "-fixing leaf algae such as Anabaena, Hostoc and other species can _{fix molecular atmospheric nitrogen (Ii 0} ), ie convert it into organic compounds such as amino acids, peptides and the like (1 - 4) Many of these organisms excrete nitrogen-free and nitrogen-containing organic compounds such as saccharides and peptides into the nutrient solution ( 5 - 10) "" They therefore enrich the "nutrient solution with such compounds.

^: Jrünalgeii ocer other organisms can now clean up these compounds excreted by the p-fixing blue-green algae and use them as additives. Consumer.

This now opens up the possibility of both partners in a suitable Breeding system to breed together and the obtained cultured algae material for purposes of nutrition, the '.feeding i.a.ra. to make usable. . "The invention described here achieves this goal in the following ways:

Embodiment I.

In. Common Zücntungs'becicen any y-size are first Np-fixing purple algae such as z _o 3B. Anabaena or iviOstoc species grown in a suitable nutrient solution with sufficient light and ventilation. The usual algae nutrient solutions, which have been used up to now and are composed of commercially available mineral salts, are suitable for the cultivation of the Hp-fixing blue-green algae but do not develop chemically bound nitrogen (e.g. ammonium salts, nitrates, etc.).
soon the blue-green algae that fix the Eg in the nutrient solution

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If the total nitrogen excreted has reached a sufficiently high value (approx. 300 - 1000 lü-iol il / 1), a sufficiently large amount of green algae or other organisms is added to the nutrient solution rait the rilaiial gene. jJiese added organisms now also growing in egg broth and take from it Np-iixi end Llaualgen excreted otickstofi on from the ITänrlösung. In this way the growth of organisms arises in the nutrient solution, because the blue-green algae and the green algae (or other organisms) that have been added are now growing side by side, with the blue-green algae supplying the nitrogen required by the other organisms in the amount of amino acids, Deliver peptides, etc.

Un τ er sufficient light and ventilation all organisms become in the breeding oars bit; bred for optimal growth. Then the grown material is harvested and placed in moist or getrocknexem state of its further use.

In order to achieve continuous growth, it is Kweckmäuig not harvest all grown material einrial after Urreichen ties optimal growth, but at appropriate distance s always just a T. ecause it, so as to ensure that the remaining in the nutrient organisms Grow again as quickly as possible. Any liquid solution removed during harvesting is discarded and supplemented by adding an equal amount of fresh nutrient solution. In this way it is avoided that after some time there is a deficiency in the nutrient solution.

Embodiment II

The breeding of the Np-fixing ±> lukewarm algae together with green algae or other organisms is carried out as described "under embodiment I, but as a nutrient solution instead of the above-mentioned nutrient solutions composed of commercially available mineral salts, fresh water or mixtures used from fresh and sea water. The mixing ratio can be anything. In the jiegel, however, turn out to be Mixtures of about 93 parts by volume of fresh water with 2-20 parts by volume of sea water are particularly favorable.

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Embodiment III _w

The cultivation of the up-fixing blue algae together with green algae "or ο other organisms is carried out as specified under Example I or II, but the Bährlösungeii Yet, additional trace elements are added, especially iron, manganese and molybdenum salts, because these promote growth particularly promote the algae.

The main advantage of the cultivation process described here lies in its low costs: Because fresh water or mixtures of fresh and sea water are used, most of the mineral salts required for the growth of the organisms mentioned (blue algae, green algae, etc.) are available at no great cost Disposal. In particular, the elements P, S, Ha, K, Oa, Mg, etc. are already sufficiently contained in fresh or sea water, the nitrogen is obtained from the air via the Kp fixation of the blue-green algae, and the carbon comes partly from ₀ from the GOp of the air, partly from the organic compounds excreted by the blue-green algae. These elements do not have to be added to the nutrient solution in the form of expensive mineral salts. Another important advantage is that by cultivating the Itfp-fixing blue algae together with green algae or other organisms in common nutrient solutions, an increased breeding yield is achieved. It is crucial that the Hp-fixing blue-green algae supply the nitrogen that is necessary for the growth of the added green algae or the other organisms. The addition of iron, manganese and molybdenum salts is recommended because • these particularly promote the growth of algae. Bacteria grow only poorly in these nutrient solutions, which is why sterilization of the nutrient solutions is not necessary »

For the reasons mentioned, the breeding process described here is very cost-saving. It enables especially near the coast because of the easy availability of sweet and sweet Compared to the previous methods, seawater combined a mass cultivation of Kp-fixing blue-green algae that is significantly cheaper with green algae or other organisms.

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literature

1. Gr.E.iXjixGr, Ann. Aev. Plant Physiolo J, 51 (1956)

2. ¥ .ΐ) οΡ.ΓίϊΜ7ϋΚΊ ', l: itro,' en fixation in Plants, l'he Axhlone

Press, University of London, 1966

3c UoHüLIi-llHi \ ijj-3]> j, Ann.iiev.hicrobioi. 2_2.47 (19ob)

4. ¹ V / oDoP.oi'LV / iii ii ', Plant and boil, 32, 555 (197O)

5. itoU.üO'jl! , jÄritoPhycol.xW-ll. 2_, 1s5 (i963)

6. ν / .Ιι.Ρ.ΰϊΈ ', Άΐιί ¹ -, Kature (Lonaon) 2üü_, 1υ2υ (1963)

7. Β.Α., νΐχΙϊϊϋ ^, d.üen.Micro biol. 4ü, 1 (1 9 b ^)

8. G-.L · '.U ¹ UG-U, uceanogr.llar.Biol.jjinciiev. £. 195 (1960)

9. A.E.WaLjBY, "Protoplasma 65,223 (1968)

10. iioix. ^r xT ..; U} .jüK and 3.B.JJAV1S, J.expt.üot. (London) 22,546 (1971)

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Claims (1)

Dr.P.Pohl .0 Manchen 90, 12.12 "73ο Claims A method for zäclitting of algae, characterized in that da'd Έ «-fixing 31aualgae Susans are bred with green algae or other organisms in common Mälirösunken. . · 2. Process for the cultivation of l'p-fixing blue-green algae together with green algae or other organisms in common ITutrient solutions, characterized in that these nutrient solutions consist of fresh water or mixtures of fresh and sea water with any mixing ratio. 5. Method of breeding iip-fixing 31aualgae together with green algae or other organisms in common nutrient solutions, also by geiceniizeicimet that the nutrient solutions additional sour elements, especially iron, hangan and molybdenum salts are added » BO9825 / 0220