CA1277271C

CA1277271C - Process for cultivating procaryotes and eucaryotes and use of the thus-prepared cells in cosmetical, food industry and fodder supplementing compositions

Info

Publication number: CA1277271C
Application number: CA000509491A
Authority: CA
Inventors: Julia Jeney; Maria Balint; Gyula Hernadi; Jozsef Sabjan; Laszlo Dede; Magdolna Marossy; Miklos Fabian; Gabor Bozoki; Magdolna Ott
Original assignee: CAOLA KOZMETIKAI ES HAZTARTASVEGYIPARI VALLALAT
Current assignee: CAOLA KOZMETIKAI ES HAZTARTASVEGYIPARI VALLALAT
Priority date: 1986-04-24
Filing date: 1986-05-20
Publication date: 1990-12-04
Anticipated expiration: 2007-12-04
Also published as: SE8601936D0; ATA110286A; CH674790A5; NL8601112A; GB2189505B; GB2189505A; SE8601936L; AT387035B; BE904733A; GB8610162D0; DE3614897A1; AU5681686A; AU583916B2; FR2598591B1; JPS62273911A; FR2598591A1

Abstract

ABSTRACT OF THE DISCLOSURE

The invention relates to a process for cultivating seaweed possessing improved biological properties as well as to cosmetic, food industry and fodder supplementing compositions containing the thus-prepared seaweed. According to the process of the invention, the seaweed is cultivated by sterilizing a culture medium containing medicinal water, mineral water or thermal water of natural origin or a mixture thereof, optionally enriched by metal compounds up to a concentration of 10-2 mole/litre as related to each metal ion and up to a concentration of 10-1 mole/litre of fluorides, under a pressure of at most 10 bars (1000 kPa) at a temperature between 100°C and 140°C, then inoculating it with a pure seaweed culture, bubbling through the culture medium a sterile air flow containing 0.5 to 20% by volume of carbon dioxide at room temperature, separating the seaweed after a cultivation lasting 7 to 30 days at 0°C to 80°C, optionally working up the thus-obtained seaweed in a conventional physical or chemical manner and optionally reacting the worked up seaweed with metal compounds until reaching a concentration of at most 10-2 mole/kg related to each metal ion.

Description

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The invention relates to a process for cultlvating seaweeds possessing improved biological properties as well as to cosmetic food industry and fodder supplementing compositions containing the thus-prepared seaweeds.

Seaweeds have a long time gained a widespread use for mankind.
The utilization of seaweed in cosmetics, the food industry and fodders has also come into the general use.

In the absence of a well worked out and economical cultivating process, marine seaweed are commonly used for the above purpose, although their practicability is limited as a consequence of their high fibre content. This disadvantage is enhanced by the fact that the pollution of the seas has increased ïn recent years because of their content of radioactive and carcinogenic materials, toxic heavy metals and industrial wastes which are accumulate in the organisms of the seaweed. Thus, the ~hus-obtained whole seaweed cannot be used for cosmetics, in the food industry and for nutrition. For these purposes such fractions can only be used which had been purified from the toxic and ballast materials. Such a method of purification has been ~0 reported by M. Carames de Gouvea in: Cosmetics and Toiletries, Vol. 95, pp. 47-50 ~1980).

However, the purification is an operation removing a part of the biologically active substances of the seaweed and therefore, the thus prepared fraction of the seaweed becomes less valuable ~or cosmetic and nutritional purposes rM. Jochum: Die Thalassotherapie-Algologie, Kosmetic International 1982, pp. 76-78; Zajic: Properties and Products of Algae N.Y., Plenum 1970].

The process of working-up are commonly carried out in such a way that agar, carrageenin and alginic acid are separated from the seaweed and utilized separately.

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AS a consequence of the purification processes, the valuable provitamins of the seaweed, e.g. beta-carotene, unsaturated fats and oils such as arachic and linolic acid are cracked thus, the cosmetic and food industry compositions as well as fodder supplements containing such seaweed have decreased activity. the seaweed living or cultivated in surEace sweet waters can also accumulate bacteria and virus~s in their organisms, whereby their utilization for cosmetic and nutritional purposes cannot be general.

The invention provides a process for cultivating seaweed which makes possible on one hand to eliminate the above-mentioned drawbacks and on the other hand, the thus-prepared seaweed posses an enhanced activity in comparison to the conventional seaweed.

It has been found that, on cultivating strains of seaweed in a culture medium containing sterilized mineral, medicinal or thermal water of natural origin or a mixture thereof, seaweed are obtained in the organisms of which the accumulation of toxic substances (mercury~ lead, cadmium, radioactive contaminations, bacteria, viruses, carcinogenic agents and the like) is excluded.

It was observed that useful substances such as microelements and vitamins contained in medicinal waters were enriched in the organism of seaweed prepared by the process of the invention.

The balneologic utilization of these substances has gained a high importance for several centuries.

The active ingredients and microelements of the medicinal waters are enriched in the organism of the seaweed prepared by using the process of the invention and on the other part, these substances form addition or complex compounds, respectively with the mucoproteids being present in the seaweed.

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It is unnecessary to sub~ect the seaweed obtained according to the process of the invention to any subsequent purifying or heat-treating process. Thus, the whole seaweed can be mixed with the various cosmetic or food industry compositions as well as with fodder supplements. Therefore, the active agents of the seaweed are not damaged and their complete biological value is retained.
A further and unexpected advantage of the thus-prepared seaweed consists in that the microelements indispensable for the organism and for the skin are introduced as a-ttached to mucoproteid proteins into any organism. The thus-introduced microelements are absorbed and exert their action to a higher e~tent in the human or animal organism.

The cosmetic compositions containing the thus-prepared seaweeds are useful not only for increasing the water content of the skin but also for optimizing the function of the enzymes of the organism by introducing the microelements which are present in an easily absorbable form.

This is also valid for the vitamins and oils introduced together with the seaweed. A similar action becomes effective in the cases of the food industry compositions and fodder sùpplements, too.

The cosmetic compositions of the invention promote the hydratation of the water-deficient skin and restore the cells of the ageing, wrinkled skin. When used as hair-dressing agents or tooth pastes, a roborating effect is exerted after absorption which presents an advantageous action on the development of the heir bulbs. Loosening of the teeth is also prevented.

All these outstanding and unexpected effects are due to the fact that the substances indispensable for the organism can be introduced into the human organism in a form bound to the proteins of the organism of the seaweed, whereby the extend of the utilization (bio-availability) are improved to a high degree.

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When introduced to the organism as such, the microelements are frequently not transformed to a biologically active form and cannot participa~e of the life processes.

Zinc is e.g. a component of at least thirty enzymes of the organism and thus, it plays an important role in the proteln synthesis of the skin. Zinc-containing enzymes participate of the synthesis of DNA and RNA. The transfer of information required for the synthesis of proteins is hindèred by the absence of these zinc-containing en~ymes, whereby the restoration of the skin is made difficult.

In case of a copper deficiency, the collagenic structure of the skin is damaged.

The function of the haemetopoietic organs ar0 impeded by an iron deficiency which is supported by the fact that iron compounds introduced as such cannot sufficiently be absorbsd in the - organism. By using the seaweeds of the invention, ths microelement dsficiency of the organism can be diminished to a high extend.

A particularly preferablP effect can b0 achieved by cultivating the seaweed in such a culture medium according to the invention, wherein the metal ion content is enriched by adding an additional metal compound. It is suitable to ad~ust the metal ion content of the culture medium to a concentration o~ at most lo~2 mole/litre as related to each metal ion. Several kinds of seaweed strains can be cultivated in the same culture medium at a temperature between 0C and 80C. Thereafter, a sterile air flow containing carbon dioxide is bubbled through the inoculated culture medium. The carbon dioxide concentratlon of the air is 0.5 to 20~ by volume preferably 1.5 to 5% by volume. In the course of the air bubbling, it is suitable to illuminate the culture medium by using a fluorescent lamp.

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cultivating period lasts 7 to 30 days, preferably 12 to 14 days. The seaweed obtained is separated from the culture medium and is worked up. The process of the working up can be carried out by using conventional physical or chemical methods, The aim of working-up is to disrupt a site of the cell membrane by a mechanical method or by alterating the osmotic pressure in order to obtain the cell contents in this way.

The working-up may be accomplished as follows:

- by heat treatment at 40 to 200~, under a pressure of 0 to 50 bars (0 to 5000 kPa);

- by ultrasonic treatment;

- by treating with a surface active agent (surfactant) such as sodium lauryl ethyl sulphate; or - by mechanical method, e.g. by milling in the presence of a solid vehicle.

The thus-treated, worked up seaweed can optionally further be reacted with the solution of a metal compound to a metal ion concentration of at most 10- mole/kg of the solid seaweed. The thus-obtained seaweed can be mixed to the cosmetic compositions after drying or without drying in the form of an aqueous suspension.

The cosmetic and food industry as well as fodder supplementing compositions contain the seaweed in an amount of 0.05 to 95% by weight as related to the dry substance content, in an admixture with known liquid or solid additives.

Particularly preferable compositions can be prepared by mixing optionally an active additive in an amount of 0.05 to 50% to the compositions. This active additive may be propolls, pollen or an _ 5 _ .

~2~ 7~1 other powder of plant origin, globin, egg-white, an animal protein, a plasma protein and the like.

It is preferable to mix oils, fats of plant or animal origin, surface active agents, starch flour (fecula) of plant origin, bran, as well as preservatives, solid or liquid vehicles as additives to the compositions.

Summing up, the invention relates to a process for cultivating seaweeds possessing improved biological propertie~.

~he process of the invention comprises sterilizing a culture medium containing medicinal water, mineral water, thermal water of natural origin or a mixture thereof, optionally enriched by metal compounds up to a concentration of 10-2 mole/litre as related to each metal ion and up to a concentration of 101 mole/litre of fluorides, under a pressure of at most 10 bars (1000 kPa~ at a temperature between 100C and 140C, then inoculating with a pure seaweed culture, bubbling through the culture medium a sterile air flow containin~ 0.5 to 20% by volume of carbon dioxide at room temperature, separating the seaweed after a cultivation lasting 7 to 30 days at 0C to 80~, working up the thus-obtained seaweed in a conventional physical or chemical way and optionally reacting the worked up seaweed with metal compounds to a concentration of at most 10-2 mole/kg as relative to each metal ion.

The invention also relates to cosmetic compositions containing seaweed prepared as described above in an amount of 0.05 to 95%
by weight as relative to the dry substance, optionally 0.05 to 50% by weight active additives, preferably propolis, globin, egg-white, dried powder of plant origin as well as known cosmetical additives, pre~erably oils and fats of plant or animal origin, surface active agents, preservatives, liquid or solid diluents in an amount supplementing up to 100% by weight.

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The thus-prepared cosmetic compositions can be used as skin-treating agents ~or hydrating and restoring the skin, for increasing the muscle tone as well as for making wrinkle-free th2 ageing skin. The hair-dresslng compositions of the invention are suitable to stimulate the hair-bulbs, to feed the scalp, whereas the tooth-dressing compositions according to the inventlon can be used for alleviating the gingival atrophy as well as for preventing the tooth-loosening and the dental caries.

In addition, the invenkion relates to food industry and fodder supplementing compositions containing seaweed prepared as described above in an amount of 0.05 to 95% b~ weight relative to the dry substance, optionally active additives, pre~erably hone, blood plasma, globin, corn-germ and dried powder of plant, origin in an amount of 0.05 to 50% by weight as well as additives commonly used in the food industry, suitably preservative, liquid or solid diluents in an amount supplementing up tp 100% by weight.

The foods prepared according to the invention and the supplements which can be mixed to the fodder exert a roborating action on the human and animal organism and promote an optimum functioning of the enzyme system of the organism. An eventual deficiency of the vitamins or microelements in the human and animal organism are also completed by the compositions of the invention. A
regenerating action is also effected on the deteriorated, aged organism by these compositions.

The process and composition according to the invention are illustrated in detail by the following non-limiting Examples.

Example 1 Cultivatlon of seaweed . ..

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Seven liters of a medicinal water arising from the Lukacs Bath of Budapest are filled into a seaweed-cultivating bottle and sterilized a-t 121C under an overpressur~ of 1 bar (100 kPa) for 30 minutes.

The sterile solution is inoculated by a pure culture of Scenedemus obtisiusculus then, sterile air flow containing 1.5%
by volume of carbon dioxide is bubbled through the culture medium at 25C under illumination by a luminescent lamp. After a cultivation period lasting 14 days, the seaweed are separated from the culture medium. The thus-obtained seaweed contain the valuable microelements of the medicinal water without any toxic material.

The seaweed are worked up by an ultrasonic treatment and the thus-obtained water-containing material is used in cosmetic compositions.

ExamPle_2 Cultivation of seaweed The process described in Example 1 is followed except that a medicinal water arising from Héviz is used.

ExamPle 3 Cultivation of seaweed The process described in Example 1 is followed except that a medicinal water arising from Hajduszoboszlo is used an no ultrasonic treatment is carried out.

Example 4 Cultivation of seaweed ',', , ~ , -: , , ,:

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The process described in Example 1 is followed, except that a medicinal water arising from Piestrany (Czechoslovakia) is used and after the inoculation wlth the culture, a sterile air flow containing 5~ by volume of carbon dioxide is bubbled through the culture medium at 25C under illumination by a luminescent lamp.

Example 5 Cultivation of seaweed The process described in Example 1 :LS followed, except that lo~6 g/ml of gold (III) chloride is added to the medicinal water in a sterile manner.

ExamPle 6 Cultivation of seaweed The process described in Example 1 is followed, except that 1o~6 g/ml of iron(III) chloride is added to the medicinal water in a sterile manner.

Example 7 Cultivation of seaweed The process described in Example 2 is followed, except that 10 mgflitre of lithium fluoride are added to the medicinal water-based culture medium.

Example 8 Cultivation of seaweed The process described in Example 1 is followed, except that a mineral water of Parad (Hungary) supplemented with 1 g/litre of _ g _ ~27~2`7~

potassium nitrate and o.~ g/litre of dipotassium hydrogen phosphate is used instead of the medicinal water. The p~ value of the culture medium is adjusted to 6.5 by adding 2 N
hydrochloric acid solution. the working up is carried out in a chemical way instead of the ul-trasonic treatment by mixing 4 g of seaweed with 825 g of water and 392 g of sodium lauryl ether sulphate and heating the solution to 60C.

ExamPl~ 9 Cultivation of seaweed lo The process described in Example 1 is followed, except that Chlorella pyrenoidosa is used as a strain of seaweed instead of scenedesmus obtisiusculus.

Exam~le 10 Preparation of a face and body treating composition Four g of seaweed cultivated as described in Example 1 are suspended in 412 g of water to 70C and then mixed at ~0C with a melt containing 70 g of white vaseline, 70 g of stearin monoglyceride, 82 g of cetyl alcohol, 27 g of maize-germ oil and 27 g of Tween 60 ~a trademark) sorbitan ester polyoxyethylene derivative. Thereafter, the mixture is stirred until cool and then preserved by adding 0.2% of Xathon CG (a trademark).

~y using the thus-obtained composition as a face and body treating cosmetic preparation, an excellent hydrating and skin-regenerating action can be achieved.

Example 11 Preparation of a shampoo ,.

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184 g of Alfozid KT-25 (a trademark)~ 152 g of Zala-betain ~a trademark) of 35%, 20 g of Alfid D 11 (a trademark) and 0.2% of Kathon CG (a trademark) preserving agent are added to the mixture containing 4 g of seaweed prepared as described in Example ~.
The thus-obtained product is used as a shampoo for hair-dressing.

By regular use of this shampoo the loss of hair is moderated and the fragile hair becomes shining and pliable.

Example 12 Preparation of a composition suitable for iontophoresis 1.4 ml of the seaweed suspension prepared as descri~bed in Example 8 are weighted into an ampoule of 2 ml and 0.5 g of an alcoholic solution containing 3% by weight of propolis is added. After sealing, the ampoules are sterilized.

The thus-obtained composition can be used for regenerating the ageing, wrinkled skin as well as it can be employed for an iontophoretic treatment to make pliable the skin.

ExamPle 13 Preparation of a hydrating cream Four g of seaweed cultivated and worked up as described in Example 2 are mixed with 20 ml of an alcoholic solution containing 2 % by weigh of propolis then, the process described in Example 10 is followed to give an excellent hydrating skin-treating cream which is particularly suitable for making wrinkleless the tired skin of face.

Example 14 .: .

Preparation of a skin-regenerating face and body treating composition Four g of seaweed cultivated as described in Example 3 are heated to 80C in the presence o~ 1% of sodium hydrogen carbonate with loo ml of a solution containing 5~ by weight of globin prepared from swine blood then, the process described in Example 10 is followed to give an excellent skin and body treating composition which makes pliable the skin on the regular use.

Exam~le 15 Preparation of a skin-regeneratiny body treating composition The process described in Example 14 is followed, except that instead of the globin solution, 100 ml of water and a suspension containing 5 g of alfalfa powder and 1% by weight of sodium hydrogen carbonate are used to give a skin-restoring body treating composition.

Example 16 Preparation of a skin-regenerating face and body treating composition The process described in Example 14 is followed, except that 2~ g of quail egg-white are used instead of globin to give a composition which is particularly suitable for freshing and hydrating the ageing skin.

Exam~le 17 Example for comparison The face and body treating composition according to Example 16 was prepared, however, instead of the seaweed cultivated as `!

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described in Example 3, a non-purified whole seaweed of sea water origin were mixed into the composition. ~oth compositions were controlled on groups containing 10 individuals each. The composition containing the seaweed of sea water origin caused skin spotting and skin inflammation after a treatment lasting 3 days thus, ~he use of this composition had to be stopped. The skin of the individuals treated with the composition prepared according to Example 16 became tight and wrinkleless after a regular use lasting two weeks. Skin inflammation or spotting lo were not observed.

Exam~le 18 Preparation of a tooth paste 120 g of glycerol, 7 g of alginate, 1 g of saccharin sodium, 2 g of nipagin M, 30 g of polyethylene glycol M 600, 60 g of Dentolex 684, 180 g of Dentolex 654 (a trademark) and 330 g of sorbitol are mixed together under pressure in an equipment fitted with a stirrer then, 200 g of a suspension containing 2~ by weight of seaweed prepared according to Example 7 are added. Thereafter, a mixture containing 20% by weight of sodium lauryl sulphate and 80% by weight of glycerol are added while homogenizing the mixture. The thus-obtained tooth paste is odorized by adding 1 g of menthol to give a tooth paste alleviating the gingival bleeding and the paradentosis.

Exam~le 19 Preparation of a roborating table 900 g of blood plasma powder, 10 g of seaweed prepared as described in Example 4, 90 g of potato starch and 900 g of a medicinal water arising from the Luka~cs Bath of Budapest are mixed and then granulated. After adding 4 g of magnesium :

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steara-te to the dry granulate, the obtained product is compressed to tablets.

By the regular consumption of the tablets, both very young as well as ageing individuals can be roborated.

5 EX ample 20 Preparation of a drinking juice loO g of a suspension containing 2 g of seaweed prepared as described in Example 8 are added to 900 g of a fibrous apple juice and the mixture is homogenized.

By regular consumption of this drinking juice, an eventual iron de~iciency of the organism is complemented and a regenerating effect is exerted on the whole organism.

Example 21 Preparation of a honey composition with an increased biological 15 activity 300 g of the seaweed consumption ~as calculated for the cry substance) prepared as described in Example 2 are mixed with a mixture containing 600 g of honey and lO0 g of apple ~uice.
After homogenizing the mixture, a composition is obtained which is particularly suitable for restoring weakened, aged and post-sickness state organisms.

ExamPle 22 Preparation of a fodder supplement To 500 g of the seaweed suspension (as calculated for the dry substance) prepared as described in Example 3, 500 g of bran are .
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portionwise added and the mixture is homo~enized. the thus prepared mixture is used as fodder supplement. By a regular use of this composition, after mixing it into the fodder of fur animals, the quality of the fur is improved.

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Claims

1. A process for the cultivation of seaweed possessing improved biological properties, which comprises sterilizing a culture medium containing medicinal water, mineral water or thermal water of natural origin or mixture thereof under a pressure of at most 10 bars (1000 kPa) at a temperature between 100°C and 140°C, inoculating the medium with a pure seaweed culture, bubbling through the culture medium a sterile air flow containing 0.5 to 20% by volume of carbon dioxide at room temperature and separating the seaweed after a cultivation lasting 7 to 30 days at 0°C to 80°C.

2. A process as claimed in claim 1, which comprises bubbling through the culture medium a sterile air flow containing 1.5 to 5% by volume of carbon dioxide.

3. A process as claimed in claim 1, which comprises working up the seaweeds by using an ultrasonic treatment.

4. A process as claimed in claim 1, which water is enriched with metal compounds up to a concentration of 10-2 mole/litre to as relates to each metal ion and up to concentration of 10-1 mole/litre of fluorides.

5. A process as in claim 1, 2 or 3, in which the seaweed is worked up to disrupt a site of the membrane to obtain the ellutants and the worked up seaweed as reacted with metal compounds until reaching a concentration of at most 10-2 mole/kg relative to each metal ion.

6. A process as claimed in claim 4, in which comprise adding fluorides and/or noble metal salts as additives to the culture medium.

7. A cosmetic composition containing seaweed prepared according to claim 1 in an amount of 0.05 to 95% by weight relative to the dry substance, and a conventional cosmetical additives.

8. A composition as in claim 7, which is as a skin treating or hair-dressing composition or a tooth paste

9. A compound as in claim 7, in which the conventional cosmetic additive is selected for oils or fats of plant or animal origin; surface active agents preservatives and liquid or solid diluents.

10. A composition as claimed in claim 7, 8 or 9, containing is a active additive in an amount of 0.05 to 50% by weight.

11. A composition as claimed in claim 7, 8 or 9, containing is a active additive selected from propolis, globin, protein, and a dried powder of plant origin in an amount of 0.05 to 50% by weight.

12. A composition as claimed in claim 7, containing 20%
by weight of globin as a active filing material.

13. A food industry or fodder supplement composition comprising seaweed prepared according to claim 1, in an amount of 0.05 to 95% by weight relative to the dry substance, and conventional supplement additives.

14. A composition as claimed in claim 13, which containing 0.05 to 50% by weight as an active additive selected from honey, globin, blood plasma and dried powder of plant origin.

15. A composition as claimed in claim 13 or 14, in which the supplement additive is selected from preservatives and liquid or solid diluents.

16. A composition as claimed in claim 13, containing as active additive 95% by weight of honey together with an aqueous suspension containing 5% by weight seaweed relative to the dry substance.

17. A composition as claimed in claim 7 or 13, including fluorides and/or noble metal salts as additives.

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