FR2598591A1 - PROCESS FOR CULTURING PROCARYOYES AND EUKARYOTICS AND USE OF THE CELLS THUS PREPARED IN COSMETIC, FOOD AND FEEDING SUPPLEMENTS - Google Patents

Abstract

THE OBJECT OF THE INVENTION IS TO CULTURE ALGAE WITH IMPROVED BIOLOGICAL PROPERTIES. ALGAE ARE GROWN BY STERILIZING A CULTURE MEDIUM CONTAINING MEDICINAL, MINERAL OR THERMAL WATER OF NATURAL ORIGIN OR THEIR MIXTURES, OPTIONALLY ENRICHED WITH METAL COMPOUNDS UP TO A CONCENTRATION FOR EACH METAL PRESSURE OF LESS THAN 10 MOLELITER, FROM 10BARS TO 100-140C; A PURE CULTURE OF ALGAE IS INOCULATED; A STERILE AIR CURRENT CONTAINING 0.5 TO 20 BY VOLUME OF CO AT ROOM TEMPERATURE IS SPRAYED; SEPARATE THE ALGAE AFTER 7 TO 30 DAYS OF CULTURE AT 0 TO 80C; TREATMENT OPTIONALLY BY A KNOWN PHYSICAL OR CHEMICAL TECHNIQUE AND TREATMENT WITH METAL COMPOUNDS TO REACH THE CONCENTRATION OF 10MOLEKG OF EACH METAL ION. INCORPORATION IN COSMETICS, FOOD PRODUCTS AND FORAGE COMPOSITIONS.

Description

L598591

  The present invention relates to a method of cultivating algae having improved biological properties as well as cosmetic, food and forage supplementation compositions containing the algae thus prepared. Algae have long been widely used by humans. The use of seaweed for cosmetics, the food industry and fodder is

also become very common.

  In the absence of a well-developed and economic process of cultivation, marine algae are commonly used for the above use although their scope of use is limited because of their high fiber content. This disadvantage is further enhanced by the fact that marine pollution has increased in recent years due to the presence of radioactive and carcinogenic materials, toxic heavy metals and industrial wastes that accumulate in algal organisms. Thus, the complete algae thus obtained can not be used for incorporation in cosmetics, products of the food industry and as a nutrition agent. For these purposes, only the fractions that have been purified of the toxic and lightening substances can be used. Such a purification process has been explained by M. Carames de Gouvea in:

  Cosmetics and Toiletries Vol 95, pp 47-50 (1980).

  However, purification is an operation that removes some of the biologically active substances from marine algae and, therefore, the thus prepared fraction of algae becomes of less interest for cosmetic and nutritional purposes (Dr. Jochum: Die Thalassotherapie-Algologie , Kosmetic International 1982, pp. 76-78; Zajic: Properties and Products of

Algae N.Y Plenum 1970).

  The treatment processes are generally carried out in such a way that the agar-agar, carrageenin and algenic acid are separated from the algae.

  marine and are used separately.

  As a result of purification processes, the valuable provitamins of algae, for example beta-carotene, unsaturated fats and oils such as arachidic and linolic acid are cracked

  so that the cosmetic compositions and food compositions as well as the forage supplements containing these algae show reduced activity.

  Algae living or cultivated in surface freshwater can also accumulate bacteria and

  in their organisms, so that their use for cosmetic and nutritional purposes can not be generalized.

  The present invention relates to a method for cultivating algae, which makes it possible, on the one hand, to eliminate the aforementioned drawbacks and, on the other hand, to obtain

  algae thus prepared having a surplus of activity compared to the known algae.

  It has been found that during the cultivation of algae strains in a culture medium containing sterilized, mineral, medicinal or thermal water of natural origin or their mixture, marine algae 25 are obtained in the organisms of which there is no evidence. accumulation of toxic substances (mercury, lead, cadmium, radioactive contaminants, bacteria, viruses, agents

carcinogens, etc.).

  Useful substances such as trace elements and vitamins in medicinal waters have been found to be enriched in the body of the body.

  algae prepared by the process of the invention.

  The balneological use of these substances has become very important for several centuries. The active ingredients and the trace elements of the medicinal waters are enriched in the organism of the algae prepared by the process of the invention and, on the other hand, these substances form compounds

  addition or complex respectively with the muco5 proteids present in the algae.

  It is unnecessary to subject the algae obtained by the process of the invention to any subsequent process of purification or heat treatment. Thus, whole algae can be mixed with various cosmetic compositions or

  as well as in forage supplements.

  As a result, the active agents of the algae are not damaged and preserve their complete biological value. Another unexpected advantage of the algae thus prepared is that the essential trace elements for the organism and for the skin are introduced together with the mucoprotein proteins to which they are attached in any organism. The trace elements thus introduced are absorbed and exert their action to a greater degree in the body of man or

of the animal.

  The cosmetic compositions containing the algae thus prepared are useful not only for increasing the water content of the skin but also for optimizing the function of the enzymes of the organism by introducing the trace elements which are present.

in an easily absorbable form.

  This is also true for vitamins

  and the oils introduced together with the algae.

  A similar action also occurs when using

  the product in the food industry or as fodder supplements.

  The cosmetic compositions according to the invention contribute to the hydration of poorly hydrated skin

  and regenerate wrinkled skin cells and age

  health. When used as a styling agent or as a toothpaste, a stimulating effect is exerted after absorption which has an advantageous action on the development of hair bulbs. It also prevents the shaking of teeth. These remarkable and unexpected effects can be explained by the fact that substances indispensable to the organism can be introduced into the body.

  in a form bound to the algae proteins of the organisms, so that the degree of utilization (bioavailability) is considerably improved.

  When introduced into the body as such, the trace elements are frequently not

  transformed into a biologically active form and 15 can not participate in the process of life.

  Zinc is for example a component contained in at least 30 enzymes of the body and therefore plays an important role in the synthesis of skin proteins. Zinc-containing enzymes participate in the synthesis of DNA and RNA. The transfer of the information necessary for the synthesis of proteins is hampered by the absence of these enzymes containing zinc, so that

  regeneration of the skin becomes difficult.

  In the case of a copper deficiency, the collagen structure of the skin is damaged.

  The function of the hematopoietic organs is hampered by the deficiency of iron which is corroborated by the fact that the iron compounds introduced as such can not be sufficiently absorbed by the body. Using the marine algae of the invention, one can

  reduce to a significant degree the deficiency of trace elements in the body.

  A particularly interesting effect can be

  obtained by culturing the algae in a culture medium according to the invention in which the content of metal ions

  This is enriched by the addition of an additional metal compound. It is appropriate to adjust the metal ion content of the culture medium to a concentration of not more than 10 mole / liter with respect to each metal ion. Several types of algal strains can be cultured in the same culture medium at a temperature between 0 and 80 ° C. A sterile air stream containing carbon dioxide is then bubbled through the inoculated culture medium. The concentration of carbon dioxide in the air is 0.5

  at 20% by volume, preferably 1.5 to 5% by volume.

  During the air splash, it is appropriate to illuminate the

  culture medium using a fluorescent lamp.

  The duration of a culture period is 7 to 30 days and preferably 12 to 14 days. We separate

  the algae obtained from the culture medium and treated.

  This method of treatment can be carried out using known physical or chemical techniques. The purpose of this treatment is to break a site of the cell membrane by a mechanical process or by a change.

  osmotic pressure to obtain the cell content by this route.

  The treatment can be carried out as follows: by heat treatment at a temperature of 40 to 200 ° C. under a pressure of 0 to 50 bar (0 to 5000 kPa); by ultrasound treatment; by treatment with a surfactant (surfactant) such as sodium laurylethylsulphate; or

  by a mechanical process, for example grinding in the presence of a solid vehicle.

  The thus treated algae may optionally be subjected to reaction with the solution of a metal compound until a metal ion concentration of not more than 10-2 mol / kg of solid algae is reached. The algae thus obtained can be mixed in a cosmetic composition after drying or without

  drying in the form of an aqueous suspension.

  Cosmetic and food industry products as well as fodder supplement compositions contain algae at a rate of 0.05% by weight, based on the dry matter content, in a mixture with liquid or solid additives. known. Particularly preferred compositions may be prepared by optionally admixing an active additive of from 0.05 to 50% with the compositions. This active additive may be propolis, pollen or another powder of vegetable origin, a globin, the

  egg white, an animal protein, a plas15 ma protein or the like.

  It is best to mix oils,

  fats of plant or animal origin, surfactants, starch flour of vegetable origin, bran as well as preservatives, solid or liquid vehicles as additives to the compositions.

  To summarize, the invention relates to a method of cultivating algae with improved biological properties. The method according to the invention consists in sterilizing a culture medium containing medicinal water, mineral water, natural thermal water or their mixture, optionally enriched with metal compounds up to a concentration of 10 -2 mol. / liter for each metal ion, under a pressure not exceeding 10 bar (1000 kPa) at a temperature between 100 and 1400C, then to inoculate it with a pure culture of algae, to be bubbled through the culture medium a stream of sterile air containing from 0.5 to 20% by volume of carbon dioxide at room temperature, to separate the marine algae after a culture of a duration of 7 to 30 days at a temperature of 0 C to 80 C, optionally treating the algae thus obtained by a known physical or chemical technique and optionally reacting treated algae with metal compounds to a concentration of not more than 10-2 mol / kg

for each metal ion.

  The invention also relates to cosmetic compositions containing algae prepared as described above in a proportion of 0.05 to 95% by weight of dry substance, optionally in the presence of 0.05 to 50% by weight of active additives, preferably propolis, globin, egg white, dried powder of plant origin as well as known cosmetic additives, mainly oils and fats of vegetable or animal origin, surfactants, preservatives, diluents

  liquids or solids in a proportion q.s.p 100% by weight.

  The cosmetic compositions thus prepared can be used as skin treatment agents for moisturizing and regenerating the skin, for increasing muscle tone as well as for aging skin free of wrinkles. The styling compositions according to the invention serve to stimulate the hair bulbs, to nourish the scalp, while the toothpaste compositions according to the invention can serve to relieve gingival atrophy as well as to prevent the shaking.

and caries of the teeth.

  In addition, the invention relates to food industry and feed supplement compositions containing algae prepared as described above in an amount of from 0.05 to 95% by weight of the dry substance, optionally active additives. preferably honey, blood plasma, globin,

  maize sprouts and a dry powder of vegetable origin in a proportion of from 0.05 to 50% by weight as well as known additives which are normally used in the food industry;

  especially preservatives, liquid or solid diluents in a proportion q.s. 100% by weight.

  The foodstuffs prepared according to the invention and the supplements which can be mixed with the fodder exert an invigorating effect on the organism of the man and the animal and contribute to an optimal functioning of the enzymatic system of the animal. 'organization. A possible deficiency of vitamins or trace elements in the human or animal body is also compensated by the compositions of the invention. Regeneration effect also occurs on an organism damaged by

  age using these compositions.

  The following examples serve to illustrate the invention without in any way limiting its scope:

EXAMPLE 1

CULTIVATION OF ALGAE

  Seven liters of medicinal water from the Budapest Lukac baths are poured into a bottle of seaweed and sterilized at 121 ° C under an overpressure.

  1 bar (100 kPa) for 30 minutes.

  The sterile solution is inoculated with a pure culture of Scenedesmus obtisciusculus, and then a stream of sterile air containing 1.5% by volume of CO2 is bubbled through the culture medium at 25 ° C with illumination by a luminescent lamp. After 14 days of culture, the algae are separated from the culture medium. The algae thus obtained contain the

  precious trace elements of medicinal water without any toxic material.

  The algae are treated with ultrasound and the material containing the water thus obtained is used in

cosmetic compositions.

EXAMPLE 2

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except that a medicinal water from Héviz is used.

EXAMPLE 3

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except that the medicinal water comes from Hajduszoboszlo and that

  does not perform ultrasonic treatment.

EXAMPLE 4

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except that a medicinal water from Piestrany (Czechoslovakia) is used and after the inoculation of the culture, the volume of CO2 is bubbled with illumination.

  a stream of sterile air containing 5% through the culture medium at 25 C by a luminescent lamp.

EXAMPLE 5

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except

  10-6 g / ml of gold (III) chloride is added to the medicinal water in a sterile manner.

EXAMPLE 6

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except sterile with medicinal water iron (III) chloride.

10-6 g / ml of

EXAMPLE 7

CULTIVATION OF ALGAE

add 10 mg / l

  The procedure is as in Example 2 except in the culture medium based on medicinal water fluoride lithium.

EXAMPLE 8

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except that the mineral water of Parad (Hungary) is added with 1 g / l of potassium nitrate and 0.2 g / l of dipotassium hydrogenphosphate instead of medicinal water. The pH of the culture medium is adjusted to 6.5 by adding a solution of binormal HCl. The treatment is carried out chemically instead of ultrasonic treatment by mixing 4 g of algae with 825 g of water and 392 g of water.

  sodium lauryl ether sulphate and heating the solution to 60 C.

l1

EXAMPLE 9

CULTIVATION OF ALGAE

  The procedure is as in Example 1 except that Chlorella pyrenoidosa is used as a strain of algae instead of Scenedesmus obtisiusculus.

EXAMPLE 10

  PREPARING A COMPOSITION FOR THE TREATMENT OF THE FACE AND BODY

  4 g of algae cultured as described in Example 1 are suspended in 412 g of water at C and then mixed at 80 ° C. with a melt containing 70 g of white petrolatum, 70 g of stearyl monoglyceride. 82 g of cetyl alcohol, 27 g of corn sprouts and 27 g of Tween 60 (polyoxyethylene sorbitan ester derivative). The mixture is then stirred until cooling and is preserved by adding 0.2% of

Kathon CG.

  When the composition thus obtained is treated as a cosmetic treatment for the treatment of the face and body, an excellent action is achieved.

  moisturizing and regenerating skin.

EXAMPLE 11

PREPARATION OF A SCHAMPOO

  184 g of Alfozide KT 25, 152 g of Zala betaine 35%, 20 g of Alfide D 11 and 0.2% of Kathon CG (preservative) are added to a mixture containing 4 g of seaweed prepared as described in

  Example 8. The product thus obtained is used as a shampoo.

  With regular use of this schampoo, hair loss is reduced and

  fragile hair becomes shiny and soft.

EXAMPLE 12

  PREPARATION OF AN APPROPRIATE COMPOSITION FOR IONOPHORESIS

  1.4 ml of the algae suspension prepared as described in Example 8 is weighed into a 2 ml ampoule and 0.5 g of an alcoholic solution containing 3% by weight of propolis is added. After sealing,

the ampoules are sterilized.

  The composition thus obtained can be used to regenerate aging wrinkled skin as well as for iontophoresis treatment to render

supple skin

EXAMPLE 13

PREPARATION OF A MOISTURIZING CREAM

  4 g of algae cultured and treated as described in Example 2 are mixed with 20 ml of an alcoholic solution containing 2% by weight of propolis, followed by the procedure described in Example 10 to obtain an excellent cream. moisturizing for the treatment of

  the skin, which is particularly suitable for removing wrinkles on the tired skin of the face.

EXAMPLE 14

  PREPARATION OF A REGENERATION COMPOSITION OF

  SKIN FOR THE TREATMENT OF THE FACE AND BODY

  4 g of algae cultured as described in Example 3 are heated at 80 ° C. in the presence of 1% of sodium hydrogencarbonate with 100 ml of a solution containing 5% by weight of globin prepared from the blood. of pork then, one proceeds as in Example 10 and one obtains an excellent composition

  treatment of the skin and the body that allows, during regular use, to make the skin supple.

EXAMPLE 15

  PREPARING BODY TREATMENT COMPOSITION FOR REGENERATING SKIN

  The procedure is as in Example 14 except that instead of the solution of globin, 100 ml of water and a suspension containing 5 g of alfalfa powder and 1% by weight of sodium hydrogencarbonate are used to obtain a composition of body treatment for

regenerate the skin.

EXAMPLE 16

  PREPARATION OF A FACE TREATMENT COMPOSITION

AND BODY TO REGENERATE THE SKIN

  The procedure is as in Example 14 except that 25 g of quail egg white is used instead of globin to obtain a composition which is

  especially suitable for refreshing and moisturizing aging skin.

EXAMPLE 17

COMPARATIVE EXAMPLE

  The facial and body treatment composition according to Example 16 is prepared, but instead of the algae grown as described in Example 3, unpurified whole seaweeds from seawater are mixed in the composition. Both compositions are tested on groups each comprising ten persons. The composition containing marine algae causes pimples on the skin and inflammation of the skin after a three-day treatment and therefore the use of the composition must be discontinued. The skin of the persons treated with the composition of Example 16 becomes tense and wrinkle-free after regular use for 2 weeks. There is no inflammation of the skin and no pimples. 0 0 '::: :: * S :. z 0. ::

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  :::: 0 25 ::? S: T ::: 0 :::::: f 0 ::: f u X 30 :::::: 00 f f: Tif: D: 0 0 :: :: ::: ::

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EXAMPLE 18

PREPARATION OF A TOOTHPASTE

  120 g of glycerol, 7 g of alginate, 1 g of sodium saccharin, 2 g of Nipagin M, 30 g of polyethylene glycol 600, 60 g of Dentolex 684, 180 g are mixed together under pressure in an apparatus equipped with a stirrer. g of Dentolex 654 and 330 g of sorbitol, then 200 g of a suspension containing

  2% by weight of algae prepared according to Example 7. Then, a mixture containing 20% by weight of sodium lauryl sulphate and 80% by weight of glycerol is added while homogenizing the mixture. The toothpaste thus obtained is perfumed by adding 1 g of menthol and a toothpaste is obtained which relieves the gingival bleeding and the periodontosis.

EXAMPLE 19

PREPARATION OF A ROBORATIC COMPRESSOR

  900 g of blood plasma powder, 10 g of algae prepared as in Example 4, 90 g of potato starch and 900 g of medicinal water from the Budapest Lukac baths are mixed and then granulated. . After adding 4 g of magnesium stearate to the dry granule, the product is compressed to form tablets.

  By regular consumption of these tablets, both very young people as well as aging people can feel invigorated.

EXAMPLE 20

PREPARATION OF ORAL JUICE

  100 g of a suspension containing 2 g of algae prepared as in Example 8 are added to 900 g of a fibrous apple juice and the mixture is homogenized. By regular consumption of this juice, one remedies a possible deficiency of iron of the organism and one exerts a regenerative effect on the organism

whole.

EXAMPLE 21

  PREPARATION OF HONEY COMPOSITION HAVING ENHANCED BIOLOGICAL ACTIVITY

  300 g of the algae composition (calculated for a dry substance) prepared as described in Example 2 are mixed with a mixture containing 600 g of honey and 100 g of ponme juice. After homogenization of the mixture, a composition is obtained which is particularly suitable for restoring weakened, aging and convalescent organisms. EXAMPLE 22

FEED SUPPLEMENT: PREPARATION

  To 500 g of an algae suspension (calculated as dry substance) prepared as in Example 3, 500 g of bran are added successively and the mixture is homogenized. The mixture thus prepared is used as a supplement to the fodder. By the regular use of this composition after its incorporation into the food of fur animals, the quality

fur is improved.

R E V E N D ICATIONS

  1. A method for cultivating algae with improved biological properties, characterized in that it comprises the steps of sterilizing a culture medium containing a medicinal water, a mineral water, a thermal water of natural origin or their mixture optionally enriched with metal compounds to a concentration of 10-2 mol / liter for each metal ion, at a pressure not exceeding 10 bar (1000 kPa) at a temperature between 100 and 140 C, and then inoculating it with a pure culture - of algae, to bubble through the culture medium a stream of sterile air containing from 0.5 to 20% by volume of carbon dioxide at room temperature, to separate the marine algae after a culture of 7 to 30 days at a temperature of 0 ° C. to 80 ° C., optionally treating the algae thus obtained by a known physical or chemical technique and optionally reacting the treated algae. s with metal compounds until a concentration of not more than 10-2 mol / kg is reached for each metal ion. 2. A process according to claim 1, characterized in that it comprises the step of bubbling through the culture medium a sterile air flow containing from 1.5 to 5% by volume

of carbon dioxide.

  3. A process according to claim 1, characterized in that it comprises the step of

treat the algae with ultrasound.

  4. Cosmetic composition, preferably skin treatment composition, styling composition, or toothpaste, characterized in that it contains marine algae prepared according to claim 5 in an amount of from 0.05 to 95% by weight. mass of dry substance, optionally together with active additives in a proportion of 0.05 to 50% by weight, preferably propolis, globin, proteins, a dry powder of plant origin as well as known cosmetic additives , preferably oils and fats of vegetable or animal origin, surfactants, preservatives, liquid or solid diluents in an amount up to

q.s. 100% by weight.

  5. Composition according to claim 4, characterized in that it contains 20% by weight of

  globin as the active ingredient of the filler.

  6. Composition for the food industry or forage supplement, characterized in that it contains algae prepared according to claim 1 at a rate of 0.05 to 95% by weight of dry substance, optionally together with additives active agents in an amount of from 0.05 to 50% by weight, preferably honey, globin, blood plasma, dry powder of vegetable origin as well as known additives, preferably preservatives, diluents. liquids or solids in an amount up to qs 100%

in weight.

  - 7.- A composition according to claim 6, characterized in that it contains, as active additive, 95% by weight of honey together with an aqueous suspension containing 5% by weight of algae relative to

to the dry substance.

  8. A process according to claims 1 to 3, characterized in that incorporates fluorides

  and / or salts of a noble metal as an additive to

  culture medium and / or compositions.

  9.- composition according to one of claims 4 to 7, characterized in that it contains

  also fluorides and / or noble metal salts as additives in the culture medium and / or

in the compositions.