AU617661B2 - A method for manufacturing cosmetical, health and body care products - Google Patents
A method for manufacturing cosmetical, health and body care products Download PDFInfo
- Publication number
- AU617661B2 AU617661B2 AU12082/88A AU1208288A AU617661B2 AU 617661 B2 AU617661 B2 AU 617661B2 AU 12082/88 A AU12082/88 A AU 12082/88A AU 1208288 A AU1208288 A AU 1208288A AU 617661 B2 AU617661 B2 AU 617661B2
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- AU
- Australia
- Prior art keywords
- metaloproteins
- mass
- metaloprotein
- health
- trace elements
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cosmetics (AREA)
Description
i PATEN OFF ICE ATENT OFFICE Pe A T E NTr o@ F 17 .6 6 i COMMONWEALTH OF AUSTRALIA Patents Act 1952 COMPLETE SPECIFICATION (Original) FOR OFFICE USE Application Number: PI0511 Lodged: 24/2/87 Class Int. Class Complete Specification Lodged: Accepted: Published: Priority: Related Art: I- r I tr
I
o( t *9 a. O 4.
40 TO BE COMPLETED BY APPLICANT Name of Applicant: Peter Frank KOTAI Address of Applicant: 16 Lillian Street, COTTESLOE, in the State of Western Australia, Commonwealth of Australia.
Laszlo Dede, 1036 Budapest, Lajos u 127, Hungary, and Dr Julius Hernadi, 1025 Budapest, Kapy Ut 46, Hungary.
Actual Inventors:
S
O* S 0 Address for Service:- Wray Associates Primary Industry House 239 Adelaide Terrace Perth Western Australia 6000.
Complete Specification for the invention entitled: "A METHOD FOR MANUFACTURING COSMETICAL, HEALTH AND BODY CARE PRODUCTS" The following statement is a full description of this invention, including the best method of performing it known to me:r 2- THIS INVENTION relates to the preparation of a range of cosmetics, health and body care products having a metaloprotein content which results from the natural biosynthesis of living organisms.
Research in the field of physiology during the past years has directed attention to the vital importance of metaloproteins. Metaloproteins regulate physiological processes, secure healthy metabolical processes and have the ability to generate cells in living matter, and are components of many enzymes. Natural metaloproteins are formed inside the body, but their quantity may not always be sufficient if the nutrients within the body, be it plant, micro organism or animal, are short in trace elements or contain trace elements in an inappropriately complexed form which results in an unsatisfactory biological utilization. The above disadvantage has been overcome in this invention by utilizing living matter, such as micro organisms, which are incorporated into active agents for cosmetics, health and body care products, and are cultured and grown in an appropriate trace element rich medium, or added or fed prior to utilization so that their composition contains an increased, optimum quantity of metaloproteins.
a4 The present invention provides a method for preparing cosmetic, health and/or body care products for external use, said method comprising: incorporating trace element enhanced nutrients into living matter, and allowing biosynthesis of the living matter to form a greater number and larger variety of metaloprotein complexes than were already present in the living matter; separating at least some of the metaloproteins from the living matter; i I I 3 transforming the separated metaloproteins into a hydrophilic form; hydrolising the hydrophilic metaloproteins with proteases in a liquid and in the presence of trace element enzyme activators; and incorporating the treated metaloprotein containing liquid into cosmetic, health and/or body care products.
The trace elements may be present in association with glycine complexes, citric acid, or other acids such as hydrochloric acid. Alternatively, the trace elements may be present in water, such as seawater, thermal springs or natural mineral water. Furthermore, the living matter to referred to above may be a living organism and may be algae, such as dunaliella salina, or may be mussels, poultry, kangaroos, cattle or the like.
In the preferred form of the invention the bio-synthesised metaloproteins are transformed into a hydrophilic form and then are hydrolised with proteases in order to make absorption through the skin possible. The hydrolysis may be performed at 15' 37'C for 1 to 24 hours with pepsin or trypsin, since they are naturally occurring enzymes of living organisms, in an aqueous system in which the protein content is 0.5 10%/mass and in the presence of the S. *-8 following trace elements in the range of 108 to 10% mass, as enzyme activators: iron, manganese, zinc, copper, silver, gold, chromium, vanadium, titanium, strontium, nickel, cobalt, molybdenum, calcium, magnesium, fluorine, bromine, iodine, sulphur, selenium and alkaline hydrocarbonate. The trace elements may be present in association with glycine complexes, citric acid or other acid, for example hydrochloric acid.
In the case of systems in the cellular state, a cellrupture may be performed before the hydrolysis.
"i 4 After sterilisation, the mixture may be reacted with hydrogen peroxide at 15' 20'C for one-hour. After the total decomposition of hydrogen peroxide the agent for cosmetic, health and body care products is soluble in water and preferably contains trace elements of no less than: -2 -3 -3 zinc 1.2 x 10 copper 2 x 10 gold 10 silver -4 -2 -8 7 x 10 iron 3 x 10 chromium 10 manganese -3 -7 -4 2 x 10, nickel 6 x 10, cobalt 5 x 10 molybdenum 6 -7 -3 7 x 10 vanadium 6 x 10 strontium 2 x 10 -2 -3 -2 calcium 10 magnesium 8 x 10 sulphur 10 2 selenium 10 fluorine 7 x 10 4 bromine 7 x 10 4 -5 iodine 10 %/mass.
(In relation to gained metaloproteins from natural biosynthesis).
6 We have manufactured a range of health and body care iI products by taking 1 to 60%/mass of the substance resulting from the above processes and adding beta carotene and/or ,ti the full cell content of dunaliella salina and/or scedenesmus obliquus and/or chlorella vulgaris in -2 quantities of 10 1%/mass, after firstly supplementing it with water of 10 76%/mass preferably containing calcium, magnesium, boron, fluorine, alkaline hydrocarbonate, silicic acid and sulphide ion. By then using well known techniques, such as the use of basic and side-substances commonly employed in the cosmetic industry, the range of health and body care products may be produced.
The invention will be better understood by reference to the following examples which are preferred embodiments of the invention.
Example 1 Dunaliella salina algae is cultured according to known techniques on a culture medium that contains Glycine K'A. complexes of co-ordination number 4 and 6, and trace r -a ,i
I
5 elements of the following quantity: nickel 10-7, vanadium 10-7, fluoride 10-4, copper 10-3, zinc 10-2, molybdenum 10-6, cobalt 10-8, iron 10-2, magnesium 10-3, boron 10-5, manganese 10-3, chromium 10-8 mass The optimum culture time is ten days at 30'C. During this time the algae incorporates the trace elements as organic complexes and metaloproteins. After that the algae is filtered out. If the culturing is conducted in 10 litre volume, 5g of modified algae is obtained. The 5g of algae is mixed into 400 mls tap water, the suspension's pH is adjusted with HC1 to 2, then the proteins are given an enzyme treatment for one hour at 37'C through the addition of 0.2g pepsin of great purity. The resulting substance is sterilised at 120'C in an autoclave.
Example 2 *0 The procedure is exactly as described in Example 1 but the trace elements listed at example 1 are used not as Glycine complexes but in a water solution that contains 2g/l citric acid and 0.lg/l HC1.
Example 3 The procedure is exactly as described in Example 1 but the trace elements listed there are supplemented with the following: silver 10-3, gold 10-3, calcium 10-2, strontium 10-3, bromide 10-4 and iodide 10-5 mass Example 4 Dunaliella salina algae is cultured according to known techniques on a culture medium that contains the following trace elements in quantity as follows: zinc 10-5, copper 10-6, gold 10-6, silver 10-6, iron 10-5, chromium 10-11, manganese 10-6, nickel 10-10, cobalt 10-7, molybdenum 10-9, vanadium 10-10, strontium 10-6, calcium 10-5, magnesium 10-5, fluoride 10-7, bromide 10-7, iodide 10-8 mass to 'form an aqueous solution which contains 7 mg/l glycine, 1 ^Mr? I 6 mg/l citric acid and 0.1 mg/l HC1. The culturing time is days at 25'C. The algae is then filtered out. A mass of the algae corresponding to 20g dry weight is suspended in HEPBURN SPA natural mineral water and the pH is adjusted with inorganic bases to 9.5. The mixture is heated to and mixed for 30 minutes at that temperature. During that time the algae cells rupture and the proteins and metaloproteins become hydrophilic.
For the purpose of activating enzymes, the solution's trace element content is adjusted, while retaining all ion proportions, to be the 10 fold of the culture medium.
After the addition of O.lg trypsine of great purity the solution is kept at 15"C for 24 hours, and is mixed thoroughly from time to time. For the purpose of ending enzyme activities the solution is heated to 95°C and kept at this temperature for one hour. Sterile filtration follows, when cool. The solution's protein content is about Example The procedure is exactly as described in Example 4 but the trace element concentration of the culture medium is adjusted to be 10-6 mass without changing the proportions.
Example 6 The procedure is exactly as described in Example 1 with the difference that mussels are cultured in the presence of trace elements in the quality and quantity described in Example 1. After this, the flesh is processed to separate the metaloproteins according to standard techniques.
Example 7 The procedure is exactly as described in Example 4 with the 5 CA. 1 Adifference that Mytilus edulis is cultured in the presence 7 Example 4 and the mussel's proteins are made hydrophilic by heating at pH 10.5.
Example 8 Poultry is kept for one month on a feed that contains trace elements in the quality and quantity described in Example 1. After one month the poultry is slaughtered, the blood collected and separated. Hydrophil protein is made from the blood plasma according to the method of the Hungarian Kiskunhalasi State Farm (Australian Patent Application No.
31264/84). From one litre of blood plasma there is obtained 60g hydrophilic protein, which, as a result of the prescribed feeding, is rich in metaloproteins. The hydrophilic protein is mixed with 1200 ml water based solution of trace elements in quantity and quality described in Example 1, 0.6g trypsin of great purity is added to the mixture and mixed for one hour at 37"C. After the enzymatic treatment the mixture is sterilised at 120'C for 3 hours.
Example 9 The procedure is exactly as described in Example 8 with the difference that poultry is substituted by kangaroo.
Example Cattle are fed for three months with a stockfeed that contains trace elements of quantity and quality as listed in Example 4. The animals are slaughtered using sterile piped-knife blood-letting method and the blood is separated. Hydrophil protein is manufactured from the blood plasma according to the method of the Hungarian Kiskunhalas State Farm, (Australian Patent Application No.
31264/84). 20g hydrophilic protein is suspended in 380g Colbrook Springs natural mineral water and in order to foster enzyme activity the trace element concentration is "*jv gr a i i 8 adjusted as described in Example 4 for that purpose. The mixture is heated to 95"C and mixed at this temperature for one hour. After cooling O.lg trypsin of great purity is added and mixed at 30°C for 5 hours. The resulting solution's ion content is complemented with sulphide and selenite ions to be 10-2 and 10-6 mass respectively. In order to end enzyme activity the solution is heated to and kept at this temperature while mixing. Sterilising filtration follows when cool. The solution is treated with 6% hydrogen peroxide until the colour stabilises. The surplus hydrogen peroxide decomposes in two days at 15' after which time the solution can be used in cosmetic products as an active agent.
Example 11 St The procedure is exactly as described in Example 10 with the difference that the stockfeed's or additive's trace jI element concentration is adjusted to 1.0 mass without changing proportions.
Example 12 The procedure is exactly as described in Example 10 with the difference that the enzyme activist trace elements' concentration is adjusted to 10-8 mass without changing proportions.
Example 13 The procedure is exactly as described in Example 10 with f the difference that the enzyme activist trace elements' concentration is adjusted to 10 mass without changing proportions.
Example 14 The procedure is exactly as described in Example 10 with the difference that sterilisation is done by heat.
k4E W) 't i- 9 Example The procedure is exactly as described in Example 10 with the difference that no hydrogen peroxide treatment is employed.
Example 16 The procedure is exactly as described in Example 10 with the difference that the protein content of the enzymatic hydrolysis' substrate is increased to 8%.
END PRODUCT FORMULATIONS Example 17 Manufacture of cosmetic face cream:
S*
S.C
A. Emulgator Cetyl alcohol Anhydr. lanoline Cetiol V Mygliol 812
IPH
Preservative B. Veegum susp.
Glycerine Water Active agent as in Example 4 Preservative C. Fragrance 0.3% 21.5% 36.5% 10.0% 0.3% 0.4% Phase A is heated to 70'C under mixing. Phase B that has been previously heated to 70'C is added in instalments to phase A, under intensive mixing. Slow cooling follows, "'i ;pv vtll Oi lr f~r x while still mixing. At 40'C fragrance is added. After room temperature has been reached the cream is packaged into tubes. The end product is an excellent moisturising and nourishing face cream with regenerative qualities.
Example 18 The procedure is exactly as described in Example 17 with the difference that the active agent from the Example 4 is supplemented with 2 x 10-2 mass beta carotine and cell content of algae chlorella vulgaris in quantity of 0.5 mass Example 19 The procedure is exactly as described in Example 17 with the difference that the active agent from the Example 4 is supplemented with 1.0 mass beta carotine.
Example The procedure is exactly as described in Example 17 with the difference that the for the cream's manufacture 60.0 mass active agent from Example 4 is used.
Example 21 Manufacture of Body Milk: A. Emulgator 5.00% Paraffin oil 5.00% IPP 4.00% Cetiol V 3.00% B. HOE S 2793 0.40% Water 76.40% Active agent as in Example 10 5.00% Preservative 0.40% C. Fragrance 0.80% S- 11- HOE S 2793 is continuously mixed with water and preservative until a clear gel forms.* Active agent as described in Example 10 is mixed to the gel and the mixture is heated to 60'C. The fatty phase, that had been separately heated to 60'C is quickly introduced to the former under mixing. Mixing continues while cooling proceeds, and at 40'C fragrance is added. The end result is a nourishing body care product of outstanding quality which harmonises the functions of the skin.
Example 22 The procedure is exactly as described in Example 21 with the difference that 1.0% is used form the active agent described in Example I t Example 23 The procedure is exactly as described in Example 21 with the difference that the active agent from Example 10 is supplemented with 0.2 mass of total cell content of algae Dunaliella salina and scedenesmus obliquus respectively.
Example 24 i The procedure is exactly as described in Example 21 with ,0 the difference that in the body milk's formulation 65% of the water is substituted with water containing in 0.1 mass calcium, magnesium, manganese, iron, boron, fluoride, alkalic hydrocarbonates, silicic acid, sulphate and sulphide ions.
Example Health care massage cream for relieving muscular pain.
i i z f *t ft
C/*
T4 i 12 A. Emulgator 15.00% paraffin oil 25.00% Carrot oil 0.20% BHT 0.02% B. Water 28.88% Active agent as in Example 7 30.00% Preservative 0.60% C. Fragrance 0.30% Phase A is heated to 65'C. Phase B that has been heated to is introduced to phase A under mixing, in small instalments. Mixing continues while cooling proceeds. At fragrance is added. The resulting product is an o excellent sport cream vitamin rich, of pain relieving qualities and has a regenerative effect on muscles.
*t Example 26 The procedure is exactly as described in Example 25 with the difference that 10% of the water is substituted with water containing ions as listed in Example 24 in 2.0 mass e
Claims (16)
1. care I t I. LIp A method for preparing cosmetic, health and/or body products for external use, said method comprising: incorporating trace element enhanced nutrients into living matter, and allowing biosynthesis of the living matter to form a greater number and larger variety of metaloprotein complexes than were already present in the living matter; separating at least some of the metaloproteins from the living matter; transforming the separated metaloproteins into a hydrophilic form; hydrolising the hydrophilic metaloproteins with proteases in a liquid and in the presence of trace element enzyme activators; and incorporating the treated metaloprotein containing liquid into cosmetic, health and/or body care products.
2. A method according to claim 1 wherein the living matter is a living organism.
3. A method according to claim 1 or claim 2 wherein the trace element enhanced nutrients are such as sea water, thermal springs and natural mineral water.
4. A method according to any one of the preceding claims R wherein the nutrients comprise 10 to 10 mass of the trace elements. A method according to any one of claims 1 to 4 wherein the trace elements are selected from zinc, copper, gold, silver, iron, chromium, manganese, nickel, cobalt, -1'~A 1&x ~nfL' eu ~r 1 -14 molybdenum, vanadium, strontium, calcium, magnesium, sulphur, selenium, fluorine, bromine, iodine, titanium or alkaline hydrocarbonate.
6. A method according to claim 5 where the trace elements comprise glycine complexes.
7. A method according to any of one of the preceeding claims wherein the nutrients comprises culture medium or stockfeeds.
8. A method according to any one of the preceeding claims wherein the living matter is selected from algae, mussels, poultry and mammals.
9. A method according to any one of the preceding claims Swherein the metaloproteins are transformed into a hydrophilic form by the action of acid or base. A method according to any one of the preceding claims wherein the proteases are selected from pepsin or trypsin.
11. A method according to any one of the preceding claims whereby separation of the metaloprotein is by cell rupture.
12. A method according to any one of the preceding claims wherein the hydrolised metaloprotein containing liquid is sterilized and then is reacted with hydrogen peroxide.
13. A method according to any one of the preceeding claims wherein the separated metaloproteins contain trace elements of no less than: -2 -3 -3 zinc 1.2 x 10 copper 2 x 10 gold 1 x 10 silver 7 x 4 iron 3 x 10 2 chromium 1 x 10 manganese 2 x 10-3 -7 -4 -6 nickel 6 x 10 cobalt 5 x 10 molybdenum 7 x 10 -7 3 2 vanadium 6 x 10 strontium 2 x 10 calcium 1 x 10 -3 -2 -6 magnesium 8 x 10 sulphur 1 x 10 selenium 1 x 10 15 4 4 5 fluoride 7 x 10, bromide 7 x 10 4 iodide 1 x 10 mass
14. A cosmetic, health or body care product produced in accordance with a method according to any one of claims 1 to 13. A product according to claim 14 wherein the metaloprotein is present in amounts of 1 to
16. A product according to claims 14 or 15 wherein the product optionally comprises beta carotine in the range of -2 1 to 1 x 10 mass and/or the total cell content of dunaliella salina, and/or scedenesmus obliquus and/or chlorella vulgaris. t
17. A product according to any one of claims 14 to 16 wherein the product comprises 10 to 76% water and 0.1 to 2% I of a compound selected from calcium, magnesium, boron, I fluorine, alkaline hydrocarbonate, silicic acid and sulphide ions.
18. A method according to claim 1 substantially as herein described with reference to any one of the Examples.
19. A product according to claim 14 substantially as herein described with reference to any one of the Examples. DATED this TWENTIETH day of SEPTEMBER 1991 PETER FRANK KOTAI Applicant WRAY ASSOCIATES Perth, Western Australia, Patent Attorneys for the Applicant. c .i 1
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPI0511 | 1987-02-24 | ||
AUPI051187 | 1987-02-24 |
Publications (2)
Publication Number | Publication Date |
---|---|
AU1208288A AU1208288A (en) | 1988-08-25 |
AU617661B2 true AU617661B2 (en) | 1991-12-05 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU12082/88A Expired AU617661B2 (en) | 1987-02-24 | 1988-02-22 | A method for manufacturing cosmetical, health and body care products |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU3551084A (en) * | 1983-10-17 | 1985-05-07 | Caola Kozmetikai Es Haztartasvegyipari Vallalat | Process for the preparation of medicinal cosmetic composition |
AU4549985A (en) * | 1984-07-13 | 1986-02-10 | Economix Kozgazdasz Egyetemi Kisszovetkezet | Process for the preparation of a pharmaceutical composition influencing the tissue metabolism and having a regenerating action |
AU5681686A (en) * | 1986-04-24 | 1987-11-05 | Caola Kozmetikai Es Haztartasvegyipari Vallalat | Process for cultivating procaryotes and eucaryotes and use of the thus-prepared cells in cosmetical, food industry and fodder supplementing compositions |
-
1988
- 1988-02-22 AU AU12082/88A patent/AU617661B2/en not_active Expired
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU3551084A (en) * | 1983-10-17 | 1985-05-07 | Caola Kozmetikai Es Haztartasvegyipari Vallalat | Process for the preparation of medicinal cosmetic composition |
AU4549985A (en) * | 1984-07-13 | 1986-02-10 | Economix Kozgazdasz Egyetemi Kisszovetkezet | Process for the preparation of a pharmaceutical composition influencing the tissue metabolism and having a regenerating action |
AU5681686A (en) * | 1986-04-24 | 1987-11-05 | Caola Kozmetikai Es Haztartasvegyipari Vallalat | Process for cultivating procaryotes and eucaryotes and use of the thus-prepared cells in cosmetical, food industry and fodder supplementing compositions |
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AU1208288A (en) | 1988-08-25 |
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