AU2021103932A4 - Aloe barbadensis nutrient fortifier and application thereof - Google Patents

Aloe barbadensis nutrient fortifier and application thereof Download PDF

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AU2021103932A4
AU2021103932A4 AU2021103932A AU2021103932A AU2021103932A4 AU 2021103932 A4 AU2021103932 A4 AU 2021103932A4 AU 2021103932 A AU2021103932 A AU 2021103932A AU 2021103932 A AU2021103932 A AU 2021103932A AU 2021103932 A4 AU2021103932 A4 AU 2021103932A4
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parts
nutrient
sulfate
aloe barbadensis
fortifier
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Xueqin XIAO
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Enshi Tujia And Miao Autonomous Prefecture Central Hospital
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2

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  • Agronomy & Crop Science (AREA)
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  • General Health & Medical Sciences (AREA)
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Abstract

OF THE DISCLOSURE The present disclosure provides a nutrient fortifier of Aloe barbadensis and its application. Its active ingredients comprise nutrient chelating agents, trace elements and biostimulants; nutrient chelating agents include humic acid, biochemical fulvic acid, compound amino acids and ethylene dichloride. Trace elements are inorganic salts containing zinc, manganese, copper, magnesium, iron, sodium, molybdenum, boron, potassium and phosphorus. Biostimulants comprise maltitol and fish protein; and provide an application of it to promote for growing of Aloe barbadensis and/or improving the quality of Aloe barbadensis. By combining nutrient chelating agents, trace elements and biostimulants, etc., diluting with water and spraying on the leaves of Aloe barbadensis, the present disclosure can effectively increase the content of aloin, aloe polysaccharides and amino acids and increase the average weight of Aloe veraleaves improve gel yield, essential trace element content and antioxidant enzyme activity unit.

Description

ALOE BARBADENSIS NUTRIENT FORTIFIER AND APPLICATION THEREOF TECHNICAL FIELD
[01] The present disclosure relates to the technical field of cultivation of aloe, and more specifically, to a nutrient fortifier of Aloe barbadensis and its application.
BACKGROUNDART
[02] Aloe barbadensis belongs to the perennial evergreen fleshy herb of Aloe in the Liliaceae family. It has biological effects such as antibacterial, anti-inflammatory, anti-radiation, anti-oxidation, strengthening immunity, promoting tissue healing, regulating blood sugar, and protecting liver.
[03] Chinese medicine believes that aloe vera can purge the pathogenic fire, detoxify, dissipate blood stasis, have effects on killing insects, red eyes, constipation, white turbidity, hematuria, epilepsy in children, malnutrition, burns, women's amenorrhea, hemorrhoids, scabies, carbuncle, furuncle, swelling, toxin and traumatic injury, et al.; modem medicine has confirmed that aloe contains 75 elements, has antiseptic, anti-inflammatory, moisturizing and beautifying, invigorating the stomach and venting, strengthening the heart and promoting blood circulation, immunity and regeneration, immunity and anti-tumor, detoxification, anti-aging, analgesic and calming, and sun protection.
[04] In detail, the functions of aloe include:
[051 Anti-aging: The mucus in Aloe vera is based on arboran A Baloe mannanaloetin and other polysaccharides as the core component. Mucus is an important component to prevent cell aging and treat chronic allergies. Mucin exists in the muscles and gastrointestinal mucosa of the human body, make tissues elastic. If mucin is insufficient, muscles and mucous membranes will lose elasticity and become stiff and aging; if mucin is insufficient, the cells that make up the human body will gradually weaken and lose the ability to defend against germs and viruses; in addition, mucin can also strengthen the body and strengthen the sperm.
[06] Promoting healing: Mouse back Aloe vera can promote the healing of artificial wound, Rabbit aloe vera can shorten the healing days of artificial conjunctival edema Aloe vera juice preparation has a protective effect on skin wounds, bums and local X-ray irradiation.
[07] Strengthening the heart and promoting blood circulation: the calcium isocitrate in aloe vera can strengthen the heart, promote blood circulation, soften hardened arteries, reduce cholesterol content, expand capillaries, make blood circulation unblocked, reduce cholesterol levels, reduce the burden on the heart, and maintain blood pressure normal, purge away "toxins" from the blood.
[08] Immunity and regeneration: Aloin A, trauma hormone and glycan peptide mannose (Ke-2) have the functions of anti-viral infection, promoting wound healing and recovery, anti-inflammatory and sterilizing, absorbing heat and reducing swelling, softening the skin, and maintaining cell vitality. The combination of curdlan and callic acid also has wound healing activity.
[091 Beauty value: The polysaccharides and multiple vitamins contained in aloe have good nutrition, moisturizing and whitening effects on human skin. Aloe barbadensis Miller is the most suitable fresh aloe leaf for direct beauty, namely Aloe barbadensis. It has the properties of astringent, softening, moisturizing, anti-inflammatory and bleaching of the skin; it also has the effect of relieving hardening, keratinization and repairing scars, not only prevent small wrinkles, pouches, and sagging skin, and keep the skin moist and tender. Moreover, it can also treat skin inflammation. It also has a good effect on acne, freckles, acne, bums, cuts, insect bites, etc.; for hair it is also effective, keeping hair moist and smooth and preventing hair loss.
[10] Selenium is a trace element necessary for human and animal life, and it is also a beneficial element needed by plants. Selenium has a positive effect on plant growth, development, metabolism and stress resistance. Plants can absorb inorganic selenium and convert it into organic selenium. Selenium has a variety of physiological functions such as anti-oxidation, elimination of free radicals, and enhancement of the body's immunity. It also plays an extremely important role in detoxification of heavy metals, protection of myocardium and cardiovascular systems, and anti-cancer.
[111 Although the nutrient content of Aloe barbadensis is higher than other aloes, for in-depth development, it lacks organic selenium compounds, has low content of essential amino acids and essential trace elements, low content and activity of antioxidant enzymes, low content of aloin and polysaccharides in aloe. The problem of in-depth development of the product limits the function and value.
SUMMARY
[12] Preferred embodiments of the present invention seek to provide a nutrient fortifier of Aloe barbadensis and its application.
[13] According to an aspect of the present invention, there is provided:
[14] An Aloe barbadensis nutrient fortifier, the active ingredients of which comprise nutrient chelating agents, trace elements and biostimulants.
[15] Specifically, in the above technical solution, the nutrient chelating agent is composed of the following components by weight:
[16] 5 to 30 parts of humic acid, 5 to 30 parts of biochemical fulvic acid, 5 to 30 parts of compound amino acid and 0.5 to 5 parts of ethylenediaminetetraacetic acid.
[171 Specifically, in the above technical solution, the trace element is an inorganic salt containing zinc, manganese, copper, magnesium, iron, sodium, molybdenum, boron, potassium, and phosphorus, and the added weight of the trace element is the nutrient 0.4 to 0.65 times of chelating agent.
[18] Specifically, in the above technical solution, the biostimulant comprises:
[19] 0.5 to 3 parts of maltitol and I to 3 parts of fish protein.
[201 In detail, in the technical solution of the present disclosure, by adding trace elements, a nutrient chelating agent composed of three major phytonutrients of humic acid, biochemical fulvic acid, and complex amino acids and a chelating agent ethylenediaminetetraacetic acid (EDTA) is added at the same time. Which not only increases nutrients but also plays a role in chelating trace elements, so that inorganic trace elements are organicized, thereby increasing nutritive macro elements and functional trace elements, especially activating antioxidant enzymes; in addition, it stimulates and promotes the growth of Aloe barbadensis and the synthesis and release of antioxidant enzyme precursors by adding biological hormones (Maltitol and fish protein).
[21] Further, in the above technical solution, the trace element is composed of the following components by weight:
[221 Zinc sulfate 3 to 6 parts, manganese sulfate 1 to 4 parts, copper sulfate 2 to 5 parts, magnesium sulfate 2 to 5 parts, ferrous sulfate 3 to 6 parts, sodium selenite 0.2 to 0.5 parts, ammonium molybdate 1to 3 parts and 1 to 4 parts of boric acid, 2 to 5 parts of dipotassium hydrogen phosphate and 0.5 to 2 parts of potassium dihydrogen phosphate.
[231 Still further, in the above technical solution, the Aloe barbadensis nutrition fortifier further comprises a surfactant and a wetting penetrant.
[24] Specifically, in the above technical solution, the wetting penetrant is composed of 1 to 3 parts of sodium dodecylbenzene sulfonate and 0.5 to 3 parts of sodium nitrophenolate.
[251 In detail, in the technical solution of the present disclosure, by adding sodium dodecylbenzene sulfonate and sodium nitrophenolate as wetting and penetrating agents, the penetration and dispersion of nutrients in the body of Aloe barbadensis can be accelerated, and the absorption and utilization of nutrients and trace elements in Aloe barbadensis can be promoted, and play a synergistic effect between trace elements.
[26] Specifically, in the above technical solution, the surfactant is a neutral surfactant, and the added amount is 3 to 5 parts.
[27] Still further, in the above technical solution, the Aloe barbadensis nutrient fortifier further comprises one or more of Tachigaren, a root development agent and a pH adjuster.
[28] In detail, in the technical solutionof the present disclosure, the root development of Aloe barbadensis is promoted by adding a root development agent (thiamine + indole acetic acid + rare earth); By adding neutral surfactant to increase the surface tension of Aloe barbadensis, enhance the adsorption capacity, reduce the leaching of nutrients, and increase the absorption of nutrients by Aloe barbadensis under the cooperation of wetting penetrant; By adding Tachigaren, enhance the leaf resistance of Aloe barbadensis; By preparing a pH buffer pair Dipotassium hydrogen phosphate and potassium dihydrogen phosphate, which maintains the pH value under weakly alkaline conditions and maintains the biological activity of trace elements, which is beneficial to the healthy growth of Aloe barbadensis.
[29] Specifically, in the above technical solution, the Tachigaren is a mixture composed of hymexazol and Taghigaren in a mass ratio of 0.8-1.2:1, and the addition amount of the Tachigaren is 1 to 3 parts.
[30] Specifically, in the above technical solution, the root development agent is composed of the following components in parts by weight: 0.2 to 0.5 parts of thiamine, 0.5 to 4 parts of indole acetic acid, and 2 to 3 parts of rare earth.
[31] Specifically, in the above technical solution, the pH adjuster is 10 wt% dipotassium hydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, and the added amount of the pH adjuster is to control its pH to 7.0-7.3.
[32] In detail, in the above technical solution, the rare earth is an inorganic salt mixture containing lanthanum, samarium and yttrium.
[33] In a preferred embodiment of the present disclosure, the rare earth is a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of (0.5-0.7): (1.2-1.5): (0.7-0.8).
[341 In a specific embodiment of the present disclosure, the Aloe barbadensis nutritional supplement is composed of the following active ingredients in parts by weight:
[35] 8 to 20 parts of humic acid, 9 to 24 parts of biochemical fulvic acid, 15 to 20 parts of compound amino acid, 1 to 3 parts of ethylenediaminetetraacetic acid (EDTA), 3 to 6 parts of zinc sulfate, 2 to 4 parts of manganese sulfate , 2 to 4 parts of copper sulfate, 3 to 4 parts of magnesium sulfate, 3 to 4.5 parts of ferrous sulfate, 0.2 to 0.5 parts of sodium selenite, 1 to 2.5 parts of ammonium molybdate, 0.6 to 2 parts of boric acid, 3 to 5 parts of dipotassium hydrogen phosphate, 0.5 to 1.2 parts of potassium dihydrogen phosphate, 1to 3 parts of maltitol, 2 to 3 parts of fish protein, 1 to 3 parts of sodium dodecylbenzene sulfonate, 2 to 3 parts of sodium nitrophenolate, 3 to 5 parts of neutral surfactant, 2 to 3 parts of Tachigaren, 0.2 to 0.5 parts of Thiamine, 0.5 to 3.5 parts of indole acetic acid, 0.5 to 0.6 parts of lanthanum chloride, 1.2 to 1.4 parts of samarium nitrate, 0.7 to 0.8 parts of yttrium sulfate and a pH adjuster; wherein the added amount of the pH adjuster is to adjust its pH to 7.2.
[36] Another aspect of the present disclosure also provides the application of the above-mentioned Aloe barbadensis nutrition fortifier in promoting the growth of Aloe barbadensis and/or improving the quality of Aloe barbadensis.
[37] Specifically, in the above technical solution, the promotion of the growth of Aloe barbadensis specifically refers to an increase in leaf weight or an increase in gel yield.
[381 Specifically, in the above technical solution, the promotion of the growth of Aloe barbadensis specifically comprises an increase in nutrient content, an increase in trace element content, an increase in selenium content, or an increase in antioxidant enzyme activity.
[39] In detail, in the above technical solution, the application is specifically that when Aloe barbadensis grows to 8-9 months, prepare the Aloe barbadensis nutrient fortifier to an aqueous solution with a concentration of 0.08-0.25 g/L, spray on both sides of the leaf, spray once every 14-16 days, and spray 3-5 times in total.
[40] Compared with the prior art, the present disclosure has the following beneficial effects:
[41] The nutrient fortifier of Aloe barbadensis provided by the present disclosure combines nutrient chelating agents, trace elements, biostimulants, etc., diluted with water and sprayed on the leaves of Aloe barbadensis, to make the content of the main nutrients aloin, aloe polysaccharides amino acids increased by 30-35%, 30-40% and -35%, especially the sulfur-containing amino acids increased significantly, and the average weight of aloe vera leaves increased by 30-40%, and the gel yield rate increased by 25-35%. The content of essential trace elements is increased by 40-45%, especially the total selenium is increased by more than 90%, the organic selenium is increased by more than 95%, the activity unit of antioxidant enzymes (SOD, GSH-Px) is increased by 30-50%, and the total antioxidant capacity is increased by more than 40%.
DETAILED DESCRIPTION OF THE EMBODIMENTS
[421 The present disclosure will be further described in detail below with reference to specific embodiments, so that those skilled in the art can understand the present disclosure more clearly.
[43] The following examples are only used to illustrate the present disclosure, but not only to limit the scope of the present disclosure.
[441 Based on the specific embodiments of the present disclosure, all other embodiments obtained by those of ordinary skill in the art without inventive work shall fall within the protection scope of the present disclosure.
[45] In the embodiments of the present disclosure, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.
[461 In the embodiments of the present disclosure, unless otherwise specified, the technical means used are all conventional means well-known to those skilled in the art.
[47] Example 1
[48] 1. Composition of raw materials
[49] Nutrient chelating agent: 10 parts of humic acid, 20 parts of biochemical fulvic acid, 15 parts of compound amino acid and 2 parts of ethylenediaminetetraacetic acid;
[50] Trace elements: 6 parts of zinc sulfate, 2 parts of manganese sulfate, 3 parts of copper sulfate, 3 parts of magnesium sulfate, 3 parts of ferrous sulfate, 0.5 parts of sodium selenite, 2 parts of ammonium molybdate, 1 part of boric acid, 4 parts of dipotassium hydrogen phosphate and 0.5 parts of potassium dihydrogen phosphate;
[511 Biostimulant: 1 part of maltitol and 3 parts of fish protein;
[52] Surfactant: 5 parts of neutral surfactant;
[53] Wetting penetrant: 1 part of sodium dodecylbenzene sulfonate and 2 parts of sodium nitrophenolate;
[54] Tachigaren: 2 parts (mixture of hymexazol and Taghigaren with a mass ratio of 1:1);
[551 Root development agent: 0.5 part of thiamine, 0.5 part of indole acetic acid and 3 parts of rare earth (a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of 0.7:1.5:0.8)
[56] pH adjuster: 10 wt% potassium dihydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, added in an amount to control its pH to 7.2.
[571 2. Preparation method
[58] Sl. Weigh the various raw materials of the nutrient chelating agent according to the ratio, and add them to 3 times the weight of clean water to fully dissolve them to obtain liquid A;
[59] S2. Weigh the various raw materials of the trace elements according to the ratio, and add them to 5 times the mass of clean water to fully dissolve them to obtain liquid
B;
[60] S3. Mix liquid A and liquid B, heat and stir and mix well, add biostimulant, surfactant, wetting penetrant, Tachigaren and root development agent according to the proportion and stir evenly, and finally add pH adjuster to adjust pH to 7.2.
[61] Among them, the sequence of steps Si and S2 can be exchanged, and they can also be carried out at the same time.
[621 Example 2
[63] 1. Composition of raw materials
[64] Nutrient chelating agent: 20 parts of humic acid, 10 parts of biochemical fulvic acid, 20 parts of compound amino acid and 1 part of ethylenediaminetetraacetic acid;
[65] Trace elements: 3.5 parts of zinc sulfate, 3 parts of manganese sulfate, 4 parts of copper sulfate, 4 parts of magnesium sulfate, 4 parts of ferrous sulfate, 0.5 parts of sodium selenite, 1 part of ammonium molybdate, 2 parts of boric acid, 5 parts of dipotassium hydrogen phosphate and 0.5 parts of potassium dihydrogen phosphate;
[66] Biostimulant: 2 parts of maltitol and 2 parts of fish protein;
[67] Surfactant: 4 parts of neutral surfactant;
[681 Wetting penetrant: 2 parts of sodium dodecylbenzene sulfonate and 3 parts of sodium nitrophenolate;
[69] Tachigaren: 3 parts (mixture of hymexazol and Taghigaren with a mass ratio of 1:1);
[70] Root development agent: 0.2 parts of thiamine, 1.3 parts of indole acetic acid and 2 parts of rare earth (a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of 0.6:1.2:0.7)
[71] pH adjuster: 10 wt% potassium dihydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, added in an amount to control its pH to 7.2.
[72] 2. Preparation method
[73] Sl. Weigh the various raw materials of the nutrient chelating agent according to the ratio, and add them to 3 times the weight of clean water to fully dissolve them to obtain liquid A;
[74] S2. Weigh the various raw materials of the trace elements according to the ratio, and add them to 5.5 times the mass of clean water to fully dissolve them to obtain liquid B;
[751 S3. Mix liquid A and liquid B, heat and stir and mix well, add biostimulant, surfactant, wetting penetrant, Tachigaren and root development agent according to the proportion and stir evenly, and finally add pH adjuster to adjust pH to 7.2.
[76] Among them, the sequence of steps Si and S2 can be exchanged, and they can also be carried out at the same time.
[77] Example 3
[781 1. Composition of raw materials
[79] Nutrient chelating agent: 15 parts of humic acid, 15 parts of biochemical fulvic acid, 15 parts of compound amino acid and 2 parts of ethylenediaminetetraacetic acid;
[80] Trace elements: 5 parts of zinc sulfate, 4 parts of manganese sulfate, 4 parts of copper sulfate, 4 parts of magnesium sulfate, 4 parts of ferrous sulfate, 0.2 parts of sodium selenite, 2 parts of ammonium molybdate, 1 part of boric acid, 3 parts of dipotassium hydrogen phosphate and 0.8 parts of potassium dihydrogen phosphate;
[81] Biostimulants: 3 parts of maltitol and 3 parts of fish protein;
[82] Surfactant: 3 parts of neutral surfactant;
[83] Wetting penetrant: 3 parts of sodium dodecylbenzene sulfonate and 3 parts of sodium nitrophenolate;
[841 Tachigaren: 3 parts (mixture of hymexazol and Taghigaren with a mass ratio of 1:1);
[85] Root development agent: 0.5 parts of thiamine, 3.5 parts of indole acetic acid and 3 parts of rare earth (a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of 0.5:1.5:0.8)
[86] pH adjuster: 10 wt% potassium dihydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, added in an amount to control its pH to 7.2.
[87] 2. Preparation method
[88] Si. Weigh the various raw materials of the nutrient chelating agent according to the ratio, and add them to 3 times the weight of clean water to fully dissolve them to obtain liquid A;
[891 S2. Weigh the various raw materials of the trace elements according to the proportion, and add them to 6 times the mass of clean water to fully dissolve them to obtain liquid B;
[90] S3. Mix liquid A and liquid B, heat and stir and mix well, add biostimulant, surfactant, wetting penetrant, Tachigaren and root development agent according to the proportion and stir evenly, and finally add pH adjuster to adjust pH to7.2.
[911 Among them, the sequence of steps S Iand S2 can be exchanged, and they can also be carried out at the same time.
[92] Comparative example 1
[93] 1. Composition of raw materials
[94] Nutrient chelating agent: 10 parts of humic acid, 20 parts of biochemical fulvic acid and 15 parts of compound amino acid;
[95] Trace elements: 6 parts of zinc sulfate, 2 parts of manganese sulfate, 3 parts of copper sulfate, 3 parts of magnesium sulfate, 3 parts of ferrous sulfate, 0.5 parts of sodium selenite, 2 parts of ammonium molybdate, 1 part of boric acid, 4 parts dipotassium hydrogen phosphate and 0.5 parts of potassium dihydrogen phosphate;
[961 Biostimulant: 2 parts of maltitol;
[971 Surfactant: 5 parts of neutral surfactant;
[98] Wetting penetrant: 1 part of sodium dodecylbenzene sulfonate and 2 parts of sodium nitrophenolate;
[99] Tachigaren: 2 parts (mixture of hymexazol and Taghigaren with a mass ratio of 1:1);
[100] Root development agent: 0.5 part of thiamine, 0.5 part of indole acetic acid and 3 parts of rare earth (a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of 0.7:1.5:0.8)
[101] pH adjuster: 10 wt% potassium dihydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, added in an amount to control its pH to 7.2.
[102] 2. Preparation method
[103] The preparation method is similar to that of Example 1, and will not be repeated here.
[104] Comparative example 2
[105] 1. Composition of raw materials
[106] Nutrient chelating agent: 25 parts of humic acid, 25 parts of compound amino acid and 2 parts of ethylenediaminetetraacetic acid;
[107] Trace elements: 3.5 parts of zinc sulfate, 3 parts of manganese sulfate, 4 parts of copper sulfate, 4 parts of magnesium sulfate, 4 parts of ferrous sulfate, 0.5 parts of sodium selenite, 1 part of ammonium molybdate, 2 parts of boric acid, 5 parts dipotassium hydrogen phosphate and 0.5 parts of potassium dihydrogen phosphate;
[108] Surfactant: 4 parts of neutral surfactant;
[109] Wetting penetrant: 2 parts of sodium dodecylbenzene sulfonate and 3 parts of sodium nitrophenolate;
[110] Tachigaren: 3 parts (mixture of hymexazol and Taghigaren with a mass ratio of 1:1);
[111] Root development agent: 0.2 parts of thiamine, 1.3 parts of indole acetic acid and 2 parts of rare earth (a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of 0.6:1.2:0.7)
[112] pH adjuster: 10 wt% potassium dihydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, added in an amount to control its pH to 7.2.
[113] 2. Preparation method
[114] The preparation method is similar to that of Example 1, and will not be repeated here.
[115] Test effect comparison:
[116] Set aside Aloe barbadensis test base in the aloe planting greenhouse, adopt 2 years 22cm seedlings for standardized cultivation, base fertilizer is organic fertilizer, and set up 6 groups in parallel (including test group 1-3, control group 1-2 and blank group). When Aloe barbadensis grows to 9 months, the prepared nutrient fortifier is prepared into an aqueous solution with a concentration of 0.15 g/L, and sprayed on both sides of the leaf, spraying once every 15 days for a total of spraying 4 times.
[117] Among them:
[118] Test groups 1-3 were sprayed with the Aloe barbadensis nutrient fortifier prepared in Examples 1-3;
[119] The control group 1-2 were sprayed with the Aloe barbadensis nutrient fortifier prepared in the control example 1-2;
[1201 The blank group was sprayed with pure water as the blank control.
[121] After the 4 sprays were completed, after another 2 months, Aloe barbadensis was collected and tested.
[122] Meanwhile, the chemical composition results disclosed in the article "The Comparative Study of Chemical Constituents on Aloe (A.vera L. var. Chinensis (Haw.) Berg and Aloe (A. barbadensis Mill.), FLAVOUR FRAGRANCE COSMETICS [J], 2000,4:7" are listed herein as a comparison group.
[123] The specific results are shown in Table 1-4 below.
[124] Table 1 test results of biomass and main nutrients
Experimental Experimental Experimental Experimental Experimental Control Blank Group group 1 group 2 group 3 group 1 group 2 group group Average leaf 156.1 158.0 160.0 142.8 139.5 108.2 98.6 weight (g)
Gel yield ( %) 66.8 65.6 67.0 54.4 56.8 51.5 49.3
Barbaloin 4.64 4.25 4.15 3.54 3.42 3.10 2.98 (m2/ka) Aloe polysaccharides 1.55 1.87 1.72 1.22 1.34 0.98 0.95
Total amino 2.03 2.12 2.05 1.34 1.52 1.28 0.97 acids (2/100a) IIIII11
[125] Table 2 test results of antioxidant nutrients and total antioxidant capacity
Experimental Experimental Experimental Control Control Blank Group group 1 group 2 group 3 group group group SOD 36.3 35.7 34.6 25.8 28.1 22.4 (U/ml ______________
GSH-Px 28.4 34.5 32.8 20.8 22.4 18.6 (U/ml) Se 60.2 64.5 57.7 24.8 12.5 0.15 (g/g) Organic selenium 58.8 63.9 55.9 35.1 25.7 0.145
Total antioxidant 269.5 270.8 266.8 174.2 182.4 156.8
[126] Table 3 amino acid content test results ( mg/kg)
Experimental Experimental Control Control Comparative blank Group Experimental group 1 group 2 group 3 group group group group
Threonine 0.0824 0.0786 0.0723 0.0718 0.0654 0.0617 0.0625
Cystine 0.0635 0.0659 0.0641 0.0351 0.0254 Not detected 0.0104
Valine 0.129 0.106 0.96 0.0954 0.101 0.0875 0.0891
Methionine 0.0527 0.06 0.054 0.0254 0.0354 0.0116 0.0151
Isoleucine 0.0821 0.0815 0.08 0.0587 0.0754 0.0647 0.0650
Leucine 0.189 0.191 0.179 0.124 0.098 0.119 0.120
Tyrosine 0.0494 0.0485 0.0467 0.0384 0.0375 0.0373 0.0405
Phenylalanine 0.0853 0.079 0.076 0.0684 0.0676 0.0672 0.0682
Lysine 0.115 0.123 0.121 0.891 0.0954 0.0824 0.0845
Histidine 0.0358 0.0367 0.0354 0.0294 0.0247 0.0239 0.0258
[127] Table 4 test results of major element and trace elements ( mg/kg)
Group Experimental Experimental Experimental Control Control Comparative Blank group group 2 group 3 group 1 group 2 group group
Iron 36.6 36.8 36.5 32.8 29.4 24.3 25.0
Copper 0.54 0.55 0.55 0.51 0.42 0.364 0.35
Manganese 6.13 6.15 6.13 5.35 5.95 4.76 4.84
Zinc 5.02 5.15 5.56 4.58 4.25 3.21 3.46
Molybdenum 0.26 0.24 0.26 0.22 0.19 0.134 0.142
Selenium 60.2 64.5 57.7 36.2 27.5 - 0.15
Magnesium 501 512 509 452 429 374 386
Phosphorus 49 51 48.5 39.8 45 35 37.5
Potassium 848 852 846 729 715 676 710
[128] Analyzing the results of Table 1-4, it can be seen that the nutrient fortifier of Aloe barbadensis provided by the embodiment of the present disclosure combines nutrient chelating agents, trace elements and biostimulants, diluted with water, and sprayed on the leaves of Aloe barbadensis. Increase the content of its main nutrients aloin, aloe polysaccharides and amino acids by 30-35%, 30-40% and 25-35% respectively. Especially the sulfur-containing amino acids increased significantly, and the average weight of aloe leaves increased by 30-40%. The gel rate of the gel is increased by 25-35%, the content of essential trace elements is increased by 40-45%, especially the total selenium is increased by more than 90%, the organic selenium is increased by more than 95%, and the activity unit of antioxidant enzymes (SOD, GSH-Px) is increased. 30-50%, the total antioxidant capacity is increased by more than 40%.
[129] Finally, the method of the present disclosure is only a preferred embodiment and is not used to limit the protection scope of the present disclosure. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present disclosure should be covered in the protection scope of the present disclosure.
[130] Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.
[131] The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

Claims (5)

WHAT IS CLAIMED IS:
1. An Aloe barbadensis nutrient fortifier, wherein, active ingredients of the fortifier comprise nutrient chelating agents, trace elements and biostimulants; the nutrient chelating agent is composed of the following components by weight: 5 to 30 parts of humic acid, 5 to 30 parts of biochemical fulvic acid, 5 to 30 parts of compound amino acid, and 0.5 to 5 parts of ethylenediaminetetraacetic acid; the trace elements are inorganic salts containing zinc, manganese, copper, magnesium, iron, sodium, molybdenum, boron, potassium and phosphorus, and the added weight of the trace elements is 0.4 to 0.65 times that of the nutrient chelating agent; and the biostimulant comprises 0.5 to 3 parts of maltitol and I to 3 parts offish protein.
2. The Aloe barbadensis nutrient fortifier according to claim 1, wherein, the trace elements are composed of the following parts by weight: Zinc sulfate 3 to 6 parts, manganese sulfate 1 to 4 parts, copper sulfate 2 to 5 parts, magnesium sulfate 2 to 5 parts, ferrous sulfate 3 to 6 parts, sodium selenite 0.2 to 0.5 parts, ammonium molybdate 1to 3 parts and 1to 4 parts of boric acid, 2 to 5 parts of dipotassium hydrogen phosphate and 0.5 to 2 parts of potassium dihydrogen phosphate.
3. The Aloe barbadensis nutrient fortifier according to claim 1 or 2, wherein, the fortifier also comprises surfactants and wetting penetrants; the wetting penetrant is composed of 1 to 3 parts of sodium dodecylbenzene sulfonate and 0.5 to 3 parts of sodium nitrophenolate; and the surfactant is a neutral surfactant, and its addition amount is 3 to 5 parts.
4. The Aloe barbadensis nutrient fortifier according to any one of claims 1 to 3, wherein, the fortifier also comprises one or more of Tachigaren, root development agent and pH adjuster; wherein, the Tachigaren is a mixture composed of hymexazol and Taghigaren in a mass ratio of 0.8-1.2:1, and the addition amount of the Tachigaren is I to 3 parts; and/or, the root development agent is composed of the following components by weight: 0.2 to 0.5 parts of thiamine, 0.5 to 4 parts of indole acetic acid, and 2 to 3 parts of rare earth; and/or, the pH adjuster is 10 wt% dipotassium hydrogen phosphate and/or 10 wt% potassium dihydrogen phosphate, and an amount of the pH adjuster added is to control the pH to 7.0 to 7.3; wherein, the rare earth is an inorganic salt mixture containing lanthanum, samarium and yttrium; and preferably, the rare earth is a mixture of lanthanum chloride, samarium nitrate and yttrium sulfate with a mass ratio of (0.5-0.7): (1.2-1.5): (0.7-0.8).
5. The Aloe barbadensis nutrient fortifier according to any one of claims 1 to 4, wherein, the fortifier comprises the following parts by weight of active ingredients: 8 to 20 parts of humic acid, 9 to 24 parts of biochemical fulvic acid, 15 to 20 parts of compound amino acid, 1 to 3 parts of ethylenediaminetetraacetic acid (EDTA), 3 to 6 parts of zinc sulfate, 2 to 4 parts of manganese sulfate , 2 to 4 parts of copper sulfate, 3 to 4 parts of magnesium sulfate, 3 to 4.5 parts of ferrous sulfate, 0.2 to 0.5 parts of sodium selenite, I to 2.5 parts of ammonium molybdate, 0.6 to 2 parts of boric acid, 3 to parts of dipotassium hydrogen phosphate, 0.5 to 1.2 parts of potassium dihydrogen phosphate, 1 to 3 parts of maltitol, 2 to 3 parts of fish protein, 1 to 3 parts of sodium dodecylbenzene sulfonate, 2 to 3 parts of sodium nitrophenolate, 3 to 5 parts of neutral surfactant, 2 to 3 parts of Tachigaren, 0.2 to 0.5 parts of Thiamine, 0.5 to 3.5 parts of indole acetic acid, 0.5 to 0.6 parts of lanthanum chloride, 1.2 to 1.4 parts of samarium nitrate, 0.7 to 0.8 parts of yttrium sulfate and a pH adjuster; and the added amount of the pH adjuster is to adjust the pH to 7.2.
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