GB2026028A - Bacterially produced dietary supplement - Google Patents

Bacterially produced dietary supplement Download PDF

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Publication number
GB2026028A
GB2026028A GB7924507A GB7924507A GB2026028A GB 2026028 A GB2026028 A GB 2026028A GB 7924507 A GB7924507 A GB 7924507A GB 7924507 A GB7924507 A GB 7924507A GB 2026028 A GB2026028 A GB 2026028A
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United Kingdom
Prior art keywords
nutrient medium
bacteria
fermentation
nitrogen
dietary supplement
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Granted
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GB7924507A
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GB2026028B (en
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SP KONSTRUKTTEKHNOLOG BJURO
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SP KONSTRUKTTEKHNOLOG BJURO
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

The dietary supplement, for humans or animals, has a total protein content of 6 to 26% by weight, a pH of 3.2 to 3.8 and a Turner acidity of 600 to 900 DEG , and is obtained by: (a) fermentation of a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, by means of a bacterial leaven consisting of thermophilic bacteria of the genus Lactobacterium or Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Esherichia coli or Pseudomonas produced by longterm symbiosis and adaptation of the bacteria to a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, and (b) separation of the culture liquid from the resulting biomass after the fermentation. Fermentation is preferably at -5 to + 15 DEG C for 1 to 11 DIVIDED 2 days, and then at 20 DEG to 50 DEG C for 1 to 11 DIVIDED 2 days.

Description

SPECIFICATION Bacterially produced dietary supplement The present invention is concerned with a bacterially produced material which is useful as a dietary supplement for humans or animals.
According to one aspect of the invention, there is provided a dietary supplement comprising a product having a total protein content of 6 to 26% by weight, a pH of 3.2 to 3.8 and a Turner acidity of 600 to 900 , the product being obtained by: (a) fermentation of a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, by means of a bacterial leaven consisting of thermophilic bacteria of the genus Lactobacterium or Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia coli or Pseudomonas produced by long-term symbiosis and adaptation of the bacteria to a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, and (b) separation of the culture liquid from the resulting biomass after the fermentation.
According to another aspect of the invention, there is provided a process of producing such a dietary supplement which comprises preconditioning a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds; introducing into the nutrient medium a bacterial leaven consisting of thermophilic bacteria of the genus Lactobacterium or Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia coli or Pseudomonas produced by long-term symbiosis and adaptation of the bacteria to a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, fermentation at a temperature of from - 5 to + 1 5 C for 1 to 1.5 days, and then at a temperature of from 20 to 50"C for 1 to 1.5 days, followed by separation of the culture liquid from the resulting biomass.
In the lower temperature stage, the mesophilic bacteria are active, while in the higher temperature stage, the thermophilic bacteria are active.
The dietary supplement according to the invention contains a range of nutrients, including aminoacids (lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine and phenylalanine), various enzymes, vitamins, carbohydrates, various low-molecular compounds and trace elements.
The dietary supplement according to the present invention can be used in either liquid state (homogenized culture liquid) or in the form of a paste or dry powder tablets). When in the liquid state, the supplement according to the present invention is generally mixed with a food-grade filler such as soya flour and/or rice flour and dried at room temperature. The dietary supplement has a particular colour and smell, depending on the composition of the nutrient medium.
When administered to human beings, the dietary supplement according to the invention caused better appetite, sleep, reduced fatigue, higher cheerfulness and enhanced working capacity.
When administered to non-human animals with foodstuffs, the dietary supplement according to the invention caused 5 to 10% weight gain, active behaviour, mobility and better-tasting meat.
In the process according to the invention, the bacterial leaven is preferably introduced into the nutrient medium in an amount of 2 to 5% by volume. As the nutrient medium use can be made of any suitable nutrient medium containing sources of carbon, nitrogen, mineral salts and trace elements.
As the protein source, there may be used, for example, milk, rennet, pork offal (such as pig stomachs), birds, eggs, fish and other suitable products of animal origin. When offal is used, it is preferably taken from healthy animals directly after slaughter and rinsed with cold water to remove foreign matter. As the source of proteins and sugars use can be made of various plant matter (more than 100 varieties have been tried including medicinal plants) such as leguminous plants, stinging nettle, dandelion, dill, caraway, eucalyptus, schizandra, valerian, calendula and red beet. Stems, leaves, roots, seeds and flower pollen can be used. Preferred carbohydrate sources are bee bread and honey, while various metal salts and oxides may be used as the source of trace elements.
A preferred nutrient medium has the following composition in percentage by weight: milk 30.0 to 35.0 rennet 2.0 to 3.0 pork offal 19.0 to 21.0 cobalt sulphate 0.002 to 0.004 poultry eggs 1.5 to 2.0 fish 1.0 to 3.0 common salt 0.8 to 1.0 ethanol 0.04 to 0.05 potassium chloride 0.4 to 0.5 marble (limestone) 1.8 to 2.0 honey 1.0 to 1.5 bee bread 0.1 to 0.15 manganese sulphate 0.002 to 0.004 lake slime 1.8 to 2.0 plant matter the balance In such a nutrient medium, the above-specified ingredients are used as the source of microflora; the plant matter, fish and eggs are used as the source of protein; honey and bee bread are used as.the source of carbohydrates; manganese sulphate, cobalt sulphate and potassium chloride serve as the source of trace elements, whereas the marble provides a buffer system and is also a source of calcium.
In order to mechanically activate the nutrient medium, the latter is preferably disintegrated at a blow speed of from 100 to 300 m/sec prior to the addition of the leaven thereto. Such disintegration of the nutrient medium makes it possible to activate the fermentation process.
In order to homogenize the resulting dietary supplement and improve quality thereof, the culture liquid is preferably also disintegrated at blow speed of from 100 to 300 m/sec after filtration thereof.
The process according to the present invention can be performed both batch-wise or continuously. As mentioned above, the culture medium is separated from the biomass after completion of fermentation. The separated biomass may be used as the leaven in a subsequent cycle of operation.
In order that the invention may be more fully understood, the following Examples in which all percentages are by weight unless indicated to the contrary, are given by way of illustration only.
EXAMPLE 1 A nutrient medium was prepared with the following composition: milk 30.0% rennet 3.0% pork offal 20.0% cobalt sulphate 0.004% poultry eggs 2.0% fish 3.0% common salt 1.0% ethanol 0.05% potassium chloride 0.5% marble 2.0% honey 1.5% bee bread 0.15% manganese sulphate 0.004% lake slime 2.0% flowers, roots, stems, leaves of leguminous crops (peas, soya, clover, locust tree, liquorice) the balance All the components were cleaned and fed to a disintegrator for blending, disintegration and activation under treatment at a blow speed of 1 70 m/sec. One ton of the resulting uniform mass was-charged into a bath, whereinto there was preliminarily added a bacterial leaven in the amount of 5% by volume of the nutrient medium. The bacterial leaven used was a mixture of thermophilic bacteria of the genus Lactobacterium and Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escheri chia coli and Pseudomonas produced by a long-term symbiosis of said microorganisms and adaptation thereof to an enriched nutrient medium.
The fermentation was conducted at 1 0 C for 28 hours, then at 40"C for 24 hours. On completion of the fermentation, the middle layer comprising the culture liquid (40% of the total volume) was drained off and the culture liquid was strained. The residual biomass was used as a bacterial leaven for the following production cycle. The strained culture liquid was disintegrated at a blow speed of 1 70 m/sec and then packaged.
The resulting dietary supplement was a homogeneous dark-brown liquid with a pH of 3.2, a Turner acidity of 600 and a total nitrogen content of 6.9%. The material contained aminoacids (lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine and phenylalanine), complexes of enzymes with a wide range of hydrolytic exoenzymes, trivial peptides, polysaccharides, vitamins and trace elements such as calcium, potassium, sodium and magnesium.
EXAMPLE 2 A nutrient medium was prepared having the following composition: milk 20.0% rennet 2.0% pig stomachs 8.0% pork offal 12.0% poultry eggs 2.0% common salt 1.0% fish 5.0% honey 1.0% bee bread 1.0% flower pollen 1.0% seeds of corn, rye and rice 6.0% cobalt sulphate 0.004% chromium oxide Cr203 0.001 % titanium oxide TiO2 0.001% potassium chloride 0.5% ethanol 0.2% plant stems, leaves, roots (stinging nettle, dandelion, dill, caraway, eucalyptus, schizandra, valerian, calendula and red beet) the balance All the components were cleaned and fed to a disintegrator for blending, disintegration and activation by way of treatment at a blow speed of 250 m/sec.One ton of the resulting uniform mass was charged into a bath, and 3% by volume of a bacterial leaven similar to that described in Example 1 was added.
Fermentation was conducted at 5"C for 25 hours, then at 35"C for 36 hours. On completion of the fermentation process the middle layer, which comprises the culture liquid (50% of the total volume) was drained off and the culture liquid was strained. The residual biomass was used as a bacterial leaven for the following production cycle. The strained culture liquid was disintegrated at a blow speed of 200 m/sec and then packaged.
The resulting dietary supplement was a brown-reddish homogeneous liquid of pH 3.8, Turner acidity 700 and total protein content 11%.
The material contained aminoacids (lysine, histidine, arginine, aspartic acid, threonine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine and phenylalanine), enzymes, vitamins, trivial peptides, polysaccharides and trace elements (such as potassium, calcium, sodium, cobalt, chromium and titanium).
EXAMPLE 3 A nutrient medium was prepared having the following composition: milk 35.0% rennet 2.0% pork offal 19.0% cobalt sulphate 0.003% poultry eggs 1.5% fish 2.5% common salt 0.8% ethanol 0.04% potassium chloride 0.4% marble 1.8% honey 1.0% bee bread 0.1% manganese sulphate 0.002% lake slime 1.8% flowers, leaves, roots, stems of leguminous plants (peas, soya, clover, locust tree, liquorice) the balance the process was carried out in a similar manner to that described above in Example 1, except that the initial fermentation was at 1 5 C for 30 hours and the subsequent fermentation was at 45 to 50"C for 28 hours.
The resulting dietary supplement was mixed with soya flour and made into a paste, which was dried at room temperature. The resulting material had a protein content of 25.4%.

Claims (7)

1. A dietary supplement comprising a product having a total protein content of 6 to 26% by weight, a pH of 3.2 to 3.8 and a Turner acidity of 600 to 900 , the product being obtained by: (a) fermentation of a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, by means of a bacterial leaven consisting of thermophilic bacteria of the genus Lactobacterium or Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicium, Escherichia color Pseudomonas produced by long-term symbiosis and adaptation of the bacteria to a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, and (b) separation of the culture liquid from the resulting biomass after the fermentation.
2. A process of producing a dietary supplement which comprises preconditioning a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds; introducing into the nutrient medium a bacterial leaven consisting of thermophilic bacteria of the genus Lactobacterium or Streptobacterium and mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia colior Pseudomonas produced by long-term symbiosis and adaptation of the bacteria to a nutrient medium containing sources of carbon, nitrogen, mineral salts and biologically active compounds, fermentation at a temperature of from - 5 to + 1 5 C for 1 to 1.5 days, and then at a temperature of from 20 to 50"C for 1 to 1.5 days, followed by separation of the culture liquid from the resulting biomass.
3. t A process according to claim 2, in which the bacterial leaven is introduced in an amount of from 2 to 5% by volume of the nutrient medium.
4. A process according to claim 2 or 3, in which the nutrient medium has the following composition, in percentage by weight: milk 30 to 35.0 rennet 2.0 to 3.0 pork offal 19.0 to 21.0 cobalt sulphate 0.002 to 0.004 poultry eggs 1.5 to 2.0 fish 1.0 to 3.0 common salt 0.8 to 1.0 ethanol 0.04 to 0.05 potassium chloride 0.4 to 0.5 marble 1.8 to 2.0 honey 1.0 to 1.5 bee bread 0.1 to 0.15 manganese sulphate 0.002 to 0.004 lake slime 1.8 to 2.0 plant matter the balance
5. A process according to any of claims 2 to 4, in which the nutrient medium is disintegrated at a blow speed of 100 to 300 m/sec prior to the introduction of the bacterial leaven thereinto.
6. A process according to any of claims 2 to 5, in which the culture liquid separated from the biomass is disintegrated at a blow speed of from 100 to 300 m/sec.
7. A process of producing a dietary supplement, substantially as herein described in any of Examples 1 to 3.
GB7924507A 1978-07-13 1979-07-13 Bacterially produced dietary supplement Expired GB2026028B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU2631404 1978-07-13

Publications (2)

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GB2026028A true GB2026028A (en) 1980-01-30
GB2026028B GB2026028B (en) 1982-09-15

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GB7924507A Expired GB2026028B (en) 1978-07-13 1979-07-13 Bacterially produced dietary supplement

Country Status (7)

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JP (1) JPS5559111A (en)
AT (1) AT370284B (en)
DE (1) DE2928376A1 (en)
FR (1) FR2430727A1 (en)
GB (1) GB2026028B (en)
NL (1) NL7905438A (en)
NO (1) NO792328L (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006097263A2 (en) 2005-03-12 2006-09-21 bitop Aktiengesellschaft für biotechnische Optimierung Orally used compatible solute containing agents

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3125797A1 (en) * 1981-06-30 1983-01-13 Rudolf Dr. Schuler Dietetic agent
FR3050460B1 (en) * 2016-04-25 2018-09-07 Soletanche Freyssinet PROCESS FOR OBTAINING CEMENTIARY MINERAL SUBSTANCE

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH274836A (en) * 1946-02-22 1951-04-30 Guenther Dr Keitel Fritz Process for the production of bacterial preparations, in particular lactic acid-producing bacteria.
JPS5341223B2 (en) * 1974-12-05 1978-11-01

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006097263A2 (en) 2005-03-12 2006-09-21 bitop Aktiengesellschaft für biotechnische Optimierung Orally used compatible solute containing agents

Also Published As

Publication number Publication date
AT370284B (en) 1983-03-10
FR2430727B1 (en) 1983-12-16
DE2928376A1 (en) 1980-04-24
GB2026028B (en) 1982-09-15
FR2430727A1 (en) 1980-02-08
ATA483679A (en) 1982-08-15
JPS5559111A (en) 1980-05-02
JPS6317046B2 (en) 1988-04-12
NL7905438A (en) 1980-01-15
NO792328L (en) 1980-01-15

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