DE2928376A1 - BIOLOGICAL PREPARATION FOR STIMULATING THE LIFE OF BIOLOGICAL OBJECTS AND METHOD FOR THE PRODUCTION THEREOF - Google Patents

Description

2328376

SHIP ν. FONER STREHU SCHÖBEL-HOPF EBBINSHAUS FINCK

description

The present invention relates to the field of microbiology, in particular it relates to a new biological one Preparation for stimulating the vital activity of biological Objects and a process for their manufacture.

The mentioned biological preparation is used in the food industry and agriculture as a product, which is the vital activity of the organism of humans and animals stimulates

Biological preparations and processes for their manufacture are currently known. For example, Ks is an enzyme preparation known, which is obtained by growing propionic acid bacteria on a nutrient medium which is made of skimmed milk and additives of a product of animal origin,

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of milk hydrolyzate, calcium chloride and microelements. The fermentaion is initially at a temperature of 20 ⁰ C, then at a temperature of 35 ° C while maintaining after each heating step and subsequent neutralization dea the culture medium with a salt solution, for example with sodium carbonate performed # The separation of the preparation by known methods (SU -PS 186941).

Ss is an enzyme preparation; known which by breeding from Bacillus me sent er ic us on a nutrient medium made from skimmed milk, Milk hydrolyzate, cabbage ferment and subsequent introduction in the Process of fermenting a fresh portion of pasteurized skimmed milk to prevent depletion of the nutrient medium is obtained. At the. At the end of the growth of the microorganism, the medium is aerated. Then it will be off the culture mixture the enzyme preparation according to one of the known Methods, for example by failure, separated. As a precipitation agent if you use the flour of legumes pretreated with alcohol and an organic acid, for example with lactic acid, the enzyme preparation obtained is optionally dried (SU-PS 186942).

The preparations mentioned do not contain sufficient quantities for the vital activity of the living organism necessary amino acids, vitamins and ferments.

A biological preparation is also known which by placing in a nutrient medium of bacterial acid acidophilic bacteria and subsequent; Fermentation is obtained. The nutrient medium consists of animal raw material,

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ORIGINAL

(Dried skimmed milk, bee larvae and bee cocoons, table salt and additions of cobalt chloride and benzoic acid.

The original .Fermentation and hydrolysis of milk proteins and the animal raw material is carried out at a 'J-'emperatur of 15 to 2O ⁰ C during 3> 6 to 38 hours. The final hydrolysis is carried out at a temperature of 40 ^° C. for about 36 to 48 hours. During the fermentation, the nutrient medium must be stirred periodically. After the fermentation, the biomass is separated. The biological preparation is separated from the biomass, which is filtered, cooled and packaged (SU-PS 282052).

.Uas mentioned procedure does not make it possible to post a A balanced biological preparation with a sufficient content of enzymes, vitamins and To obtain microelements.

Another purpose of the present invention is intensification the production of a biological preparation.

The invention was based on the object by the choice of a new bacterial acid, a nutrient medium, the change in fermentation conditions and preparation of the nutrient medium a high quality biological preparation with one that is sufficient for the Jjeben activity of the organism Amic acid content, ferments, vitamins .rad. Microelements to maintain and to intensify the production of a biological preparation.

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'INSPECTHD

The main purpose and the other purposes have been achieved. The problem was solved in that the biological Preparation; to stimulate biological activity jeKöe czaind according to a product which one

through fermentation of a nutrient that contains carbon, nitrogen

and

material sources, mineral salts contains biological agents, with a sour that comes from theriaophilic bacteria of the genus Lactobacterium, S-creptobacteriuni, from mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia Coli, Pseudomonas and through long-term symbiosis and adaptation of the mentioned microorganisms to a carbon, nitrogen content

and

Substance sources, mineral salts enriched with biological agents Mhrmedium is obtained is obtained under subsequent Separation from the biomass after fermentation obtained culture liquid j the obtained product has the following Characteristic values for: content of total protein 6 to 26 percent by weight, pH 3.2 to 3.8 acidity according to Thörner 600 to 900 °.

The biological preparation according to the invention contains amino acids (Lysine, histidine, arginine, aspartic acid, threonine, Serine, glutamic acid, proline, glycine, alanine, valine, methionine, Osoleuzine, Leucine, Syrosine, Phenylalanine), various Enzymes, vitamins, carbohydrates, various low molecular weight Compounds, micro-elements, that is, all the most important ingredients that are necessary for the vital activity of the living organism.

The application of the preparation according to the invention as

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Food causes a constancy of the composition via the cells the internal medium of the organism »makes it possible to increase the energy level of the internal medium of the organism, and combines disorders in the protein and enzymatic Substance change of the cells.

The preparation according to the invention can be applied both in liquid form (homogenized culture liquid) and in the form of paste and dry powder (tablets), the liquid biological preparation being miscible with any edible filler such as soy flour, rice flour, their mixture and at room temperature is dried. The biological preparation was tested on humans and animals (pigs, piglets, calves) as a foodstuff, stimulator of life activities of the organism. When the preparation was taken by humans, an improvement in appetite and sleep was observed, there was a decrease in the ability to be uneasy, and there was an increase in the ability to work.

When administering the biological preparation to animals altogether old the food would increase the weight of the Animals (by 5 to 10%), activation of behavior, agility, liveliness noted and it improved Flavor quality de »meat.

The subject of the study is also a process for the production of a biological preparation, which is preparation

a nutrient medium which swells carbon, nitrogen, and. ^H *

Mineral salts contains biological active ingredients, fermentation by introducing into the nutrient medium of a bacterial Sauera,

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ORIGINAL INSPECTED

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After fermentation, the resulting culture liquid is separated from the biomass, the bacterial acid used in the method according to the invention being an acid derived from thermophilic bacteria of the genus Laetobacterium, Streptobacterium, mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia CoIi, Pseudomonas exists and long-term symbiosis and adaptation of the mentioned microorganisms to a storage medium enriched in terms of the content of sources of carbon, nitrogen, mineral salts, and biological active substances is obtained, and fermentation at a temperature of minus 5 to plus 15 ° G for 24 to 36 hours and then at a temperature of plus 20 to plus 50 ⁰ C for 24 to 36 hours.

The bacterial acid is brought in moderately the nutrient medium in an amount of 2 to 5 percent by volume. Any suitable nutrient medium with the sources of carbon, nitrogen, mineral salts and. Microelements enriched nutrient medium can be used "

A nutrient medium of the following composition is expediently used: milk 30.0 to 35.0 percent by weight, abomasum 2.0 to 3.0 percent by weight, pork innards 19.0 to 21.0 percent by weight, cobalt sulfate 0.002 to 0.004 percent by weight, poultry eggs 1.5 to 2.0 weight percent, fish biting 1.0 3.0 percent by weight sodium chloride 0.8 to 1.0 percent by weight, ethyl alcohol 0.04 0.05 big Gewichtspiozent, Ealium chl ORID Q "4 0.5 GtwiohtapBozent rock, marble 1, 8 to 2.0

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- ic -

Weight percent, honey 1.0 to 1.5 percent by weight, pollen (Bee bread) 0.1 to u, 15 percent by weight, manganese sulfate 0.002 up to υ, 004 percent by weight, lake mud 1.8 to 2.0 percent by weight, Plants from the legume family everything else

The choice of the components used in the nutrient medium is determined by the fact that the above-mentioned ingredients are used mainly used as a source of the mifcroflora that plants come from the group of legumes, fish and poultry eggs as protein source, honey and pollen as a source of. Carbohydrates and the sulfuric acid salts of manganese, cobalt and Potassium chloride is used as a micro-element source, marble for the creation of a buffer system and as a calcium source.

To carry out the mechanical activation of the nutrient medium constituent components are subjected to. the nutrient medium disintegrate before introducing the acid a bottoming speed of 100 to JOO m / s.

Carrying out the disintegration of the nutrient medium makes it possible to activate the fermentation.

For homogenizing and improving the quality of the biological Preparation, the culture liquid separated from the biomass is subjected to disintegration at a stroke speed igke it from 100 to 300 o / a

The method according to the invention is carried out as follows.

Bus Texfahr ta can be used both periodically and continuously

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ORIGINAL INSPECTn = D

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borrowed.

Man. prepares a nutrient medium. Any suitable nutrient medium rich in proteins and carbohydrates which contains mineral salts and sources of microelements can be used as the nutrient medium. As a source of protein, milk / abomasum, pork entrails, poultry eggs, pish and other products of animal origin used (pig entrails, pig stomachs, etc.) can be used, which are taken from healthy animals immediately after their slaughter. Before using them, they are washed with cold water for re-consumption inigung of the foreign additions. as a source of proteins and sugars, various plants (over 100 species, including medicinal plants) as the plants of the legume family, nettle, Kuhblume, UiIl ₉ cumin, eucalyptus, lemon, valerian, common .Ringelblume, -red beets and others "are used. Stems, leaves ;," Wur-zeIn as well as plant seeds - and "flower pollen. As ~ carbohydrates one uses honey, pollen ·, Ils" source © of the microeleiaeate one uses metal salts and "metal oxides ·

"All the components of the Mahraiedium are mixed and subjected to i) the first removal of the mechanical activation what the? Fermentation" is more useful? carried out with a bacterial acid, which is generally based on an amount of 2 to 5 * volume percent _t . AEA's helm "medium, introduced w ill ·" Bas bacteria "Sauea * stellt'einen ■

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Complex of different microorganisms, the thermophilic bacteria of the (iattung Lactobacterium, Streptobacterium, the mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acid! Propionic um, Eacherichia Coil, Peeudomonas, which were adapted to the enriched nutrient medium through long-term symbiosis fermentation is carried out at a temperature of minus 5 to plus 15 ° C for 24 to 26 hours, and then at a temperature of plus 20 to plus 50 ⁰ C for 24 to 36 hours ·

During fermentation at lower temperatures (from -5 to + 15 ⁰ C) the main mass of the substrate is subjected to fermentation by mesophilic bacteria, while at higher temperatures (from + 20 ⁰ C to + 50 ⁰ C) the fermentation by thermophilic bacteria of the Sour predominates

After the fermentation has ended, the culture liquid is separated from the biomass. The biomass is used as _a bacterial S uer for the next production cycle of the production of the biological specimen ·

The separated culture liquid is filtered through cloths and can 2For the purpose of improving the quality of the biological preparations and, for the purpose of its homogenization, disintegration at a stroke speed of up to 300 m / s.

The biological preparation obtained can be in liquid form as well as in form with the addition of edible fillers of a TrocJcenproduktea can be used. The preserved biological Preparation has a specific color and a flspecific

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see rumor that from the .composition of the vegetable Raw material and the choice of other components of the nutrient medium depend.

In order to better understand the present invention the following examples for the implementation of the process for the production of a biological preparation are given.

Example 1.

Prepare a nutrient medium with the following composition: Milk 30 »0 percent by weight, abomasum 3.0 percent by weight, Pig inner ies 20, U weight percent, cobalt sulfate 0.004 percent by weight, poultry eggs 2.0 percent by weight, fish 3.0 percent by weight, table salt 1.0 percent by weight, ethyl alcohol 0.05 percent by weight, potassium chloride, 5 percent by weight, marble 2.0 percent by weight, 1.5 percent by weight, pollen (Bee bread) 0.15 percent by weight, manganese sulfate 0.004 percent by weight, lake mud 2.0 percent by weight, flowers, roots, Stems, leaves of legumes (peas, soy, clover, acacia, liquorice, everything else.

All components are cleaned and a disintegrator for mixing, crushing and activation during the treatment with an impact speed of 170 m / s The homogeneous mass obtained in an amount of 1 ton is placed in a tub in which a bacterial acid has been placed beforehand enters in a volume of 5%, based on the nutrient medium. Because bacterial acid is a complex of microorganisms, namely the thermophilic bacteria of the genus LactobaoterJ, -um, Streptobacterium, the mesophilic bacteria Streptococcus, Azotobaoterium, Shermanni, Bacteria »aoidi propionicum, Bsohtri-

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citia CoIi, Pseudomonas, obtained through long-term symbiosis of the mentioned microorganisms and their adaptation to the enriched nutrient medium.

The fermentation is carried out at a temperature of 1O ⁰ C for 28 hours and then at a temperature of 40 ⁰ C for 24 hours. When the fermentation is complete, the middle layer, which is the culture liquid, is poured off in an amount equal to 40% of the total volume. The culture liquid is filtered through cloths. The used biomass is used as bacterial acid for the next production cycle. The liquid filtered through cloths is subjected to disintegration at a blow speed of 170 m / s and then packaged.

The biological preparation obtained is a homogeneous liquid of gray-brown color J pH = 3 | 2, acidity Terner 600 °, total nitrogen content 6.9%.

The preparation contains amino acids (lysine, histidine, arginine, Aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine), Jänzym complexes with a wide range of hydrolytic Exoenzymes, simple peptides, polysaccharides, vitamins, Microelements like calcium, potassium, sodium, magnesium.

Example 2 " A nutrient medium is prepared with the following composition: milk 20.0 percent by weight, abomasum 2.0 percent by weight, pork stomach 8.0 liewiohta percent, swine flocks 12.0 percent by weight, chicken eggs 2.0 percent by weight, table salt 1.0 percent by weight, fish 5, 0 percent by weight,

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Honey 1.0 percent by weight, pollen (bee bread; 1.0 percent by weight, pollen 1.0 percent by weight, seeds of corn, hoggen, ice cream 6.0 percent by weight, cobalt sulfate 0.004 percent by weight, uhromoxyü ux-pO, 0.001 percent by weight, titanium oxide TiO ₂ 0.001 percent by weight , Potassium chloride, 0.5 ethyl alcohol 0.2 percent by weight, stems, leaves, roots of plants (nettle, cowflower, dill, caraway, eucalyptus, citronella, valerian, common marigold, beetroot) everything else.

All components are cleaned and a disintegrator for mixing, crushing and activation during the Treatment with an impact speed of 250 m / s supplied. Pie obtained homogeneous mass in the amount of 1 You put a barrel in a tub, gives 3 percent by volume bacterial Sour, analogous to that described in Example 1, .zu.

The fermentation is carried out at a temperature of 5 ⁰ C for 25 hours and then at a temperature of 35 ° O while hours "After the completion of the fermentation pouring the middle layer, which represents the cult urflüss igke it, in an amount of 50 ? » of the total volume. The culture liquid is filtered through cloths. The remaining biomass is used as bacterial acid for the next production cycle. The culture liquid filtered through cloths is subjected to disintegration at an impact speed of 200 m / s and then packaged.

The biological preparation obtained is a homogeneous liquid of gray-brown-red color5 pH ₌ 3 _# 8, acidity

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INSPECTED

Terner 700 °, total protein content 11.0%.

The preparation contains amino acids (lysine, histidine, arginine, aaparginaic acid, threonine, serine, glutamic acid, proline, Glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine), enzymes, vitamins, simple peptides, Polysaccharides, micro-elements such as potassium, calcium, sodium, Cobalt, chrome, titanium.

Example g. A nutrient medium is prepared with the following composition: milk 3> 5% by weight, abomasum 2.0% by weight, pig innards 19 »0% by weight, cobalt sulfate D, 00j5% by weight, poultry eggs 1.5% by weight, fish 2.5% by weight, table salt 0.8% by weight , Ethyl alcohol U, 04 percent by weight, potassium chloride 0.4 percent by weight, marble 1.8 percent by weight, honey 1.0 percent by weight, pollen (bees bread) 0.1 percent by weight, manganese sulfate U, 002 percent by weight, sea foam 1.8 percent by weight, flowers, roots, Stems, leaves of legumes (peas, soy, clover, acacia, liquorice) everything else.

The process is carried out analogously to Example 1. The fermentation is carried out at a temperature of 15 ^° C. for 30 hours, then at a temperature of 45 to 50 ^° C. for 28 hours. A biological preparation is obtained which is analogous to that described in Example 1. The liquid preparation obtained is mixed with soybean meal to form a paste, which is dried at room temperature until a dry biological preparation is obtained. The total protein content of the preparation is 25.4%.

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Claims (6)

PATENTANWALTS fc s7 A 8 J / SCHIFF v.FUNER STREHL SCHÜ3EL-HOPF EBBiNGHAUS FINCK MARIAHILFPLATZ 2 * 3, MUNICH 9O POST ADDRESS: POST BOX 95 Ot6O, D-SOOO MÖNCHEN EUROPEANESSIONAL REPRESENTATIVE TECHNOLOGY SPECIALIST BEFORE 95 PROFESSIONAL REPRESENTATIVES ALSANESSIONAL REPRESENTATIVES ALSANESSIONAL REPRESENTATIVES ALSANESSIONAL OFFICE »Decintegrator» ° J £% £% £££££ * "" fOner DIPL. CHEM. OR. URSULA SCHOBEL-HOPP DIPL. INQ. DIETER EBBINQHAUS DR, INQ. DIETER FINCK TELEFON (O8B) 4S3OS4 TELEX 0-23 660 AURO D TELEGRAMS AUROMARCPAT MUNICH DEA-I9579 July 13, 1979 Biological preparation to stimulate the vital activity of biological objects and process for its production Patent claims 1. Biological preparation to stimulate vital activity biological objects, characterized in that it represents a product which one by fermentation of a nutrient medium containing carbon, nitrogen springs, containing mineral salts and biological agents, with a bacterial acid that is made from thermophilic Bacteria of the genus Lactobacterium, Streptobaeterium mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia Coil, Pseudomonas exists and through long-lasting symbiosis and adaptation of said microorganisms to one according to the content of carbon, nitrogen sources, mineral salts and nutrient medium enriched with biological agents is obtained, with subsequent separation 030017/0586 'fermentation of the biomass of the culture fluids obtained, the product obtained having the following characteristics: total protein content 6 to 26 Percentage by weight, pH 3.2 to 3.8, Thörner acidity 600 to 900 °. 2. A method for Hexstellung a biological preparation according to claim 1, which contains the preparation of a nutrient medium welcnes JLohlenstoff-, nitrogen sources, mineral salts, biological active ingredients, fermentation by introducing into aas nutrient medium of a bacterial saber, separation after fermentation of the culture liquid obtained from the biomass provides, characterized in that an acid is used as a bacterial acid, which consists of thermophilic bacteria of the genus Lactobacterium, Streptobacterium, mesophilic bacteria of the genus Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, iSgcherichia CoIi, Pseudomonaa and long-term symbology Microorganisms one after the other. Content of sources of carbon, nitrogen, mineral salts and biological active ingredients enriched hair medium is obtained, and fermentation at a temperature of minus 3 to plus 1 ^ ⁰ C for 24 to 36 hours and then at a temperature of plus 20 to plus 50 ° C during 24 to 3 & hours. 030017/0586 2328376 3 · Procedure according to. Claim 2, characterized. g- β κ β n η— draws that bacterial acid is found in a multitude brings in from 2 to 5 percent by volume, based on the culture medium 4. The method according to claim 2-3 »thereby g ek e η η ζ e it means that as a flänmaedium one is Medium of the following composition is used: milk 30.0 to 35 * 0 weight percent, abomasum 2.0 weight percent, fish 1.0 to 3 »u percent by weight, table salt 0.8 to 1.0 percent by weight, Ethyl alcohol u, u4 to 0.05 weight percent, potassium chloride 0.4 to 0.5 weight percent, marble 1.8 to 2.0 weight percent, Honey 1.0 to 1.5 percent by weight pollen (bee bread) 0.1 to 0.15 percent by weight, manganese sulfate 0.002 up to 0.004 percent by weight, lake mud 1.8 to 2.0 percent by weight, Plants from the legume family everything else. 5 · The method according to claim 2-4, dad u r c h g e— k e η η ζ e ichnet that one the nutrient medium before introducing in this the bacterial acid is subjected to a disintegration at an impact speed of 100 to 300 m / s. 6. The method according to claim 2-5, as marked by Ichnet that one for the purpose of homogenization and improving the quality of the separated from the biomass Culture fluid subjected to disintegration at an impact speed of 100 to 300 m / s. 03001770588