NL8102178A - Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. - Google Patents
Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. Download PDFInfo
- Publication number
- NL8102178A NL8102178A NL8102178A NL8102178A NL8102178A NL 8102178 A NL8102178 A NL 8102178A NL 8102178 A NL8102178 A NL 8102178A NL 8102178 A NL8102178 A NL 8102178A NL 8102178 A NL8102178 A NL 8102178A
- Authority
- NL
- Netherlands
- Prior art keywords
- antigen
- reagent
- antibodies
- insoluble
- antigens
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 55
- 108091007433 antigens Proteins 0.000 title claims abstract description 54
- 102000036639 antigens Human genes 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 34
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 230000003993 interaction Effects 0.000 claims abstract description 8
- 210000002966 serum Anatomy 0.000 claims abstract description 8
- 239000012456 homogeneous solution Substances 0.000 claims abstract description 6
- 210000001124 body fluid Anatomy 0.000 claims abstract description 3
- 239000010839 body fluid Substances 0.000 claims abstract description 3
- 238000000338 in vitro Methods 0.000 claims abstract description 3
- 210000002381 plasma Anatomy 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 108090001008 Avidin Proteins 0.000 claims description 7
- 229960002685 biotin Drugs 0.000 claims description 5
- 235000020958 biotin Nutrition 0.000 claims description 5
- 239000011616 biotin Substances 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 125000001894 2,4,6-trinitrophenyl group Chemical group [H]C1=C(C(*)=C(C([H])=C1[N+]([O-])=O)[N+]([O-])=O)[N+]([O-])=O 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 229920000936 Agarose Polymers 0.000 claims description 2
- 239000003708 ampul Substances 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 239000012736 aqueous medium Substances 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract 1
- 229940046528 grass pollen Drugs 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 229920002684 Sepharose Polymers 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- HVGAOLHAWVNIQU-UFLZEWODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid;pyrrolidine-2,5-dione Chemical compound O=C1CCC(=O)N1.N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 HVGAOLHAWVNIQU-UFLZEWODSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000004579 marble Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 230000004523 agglutinating effect Effects 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 229960004784 allergens Drugs 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940043515 other immunoglobulins in atc Drugs 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL8102178A NL8102178A (nl) | 1981-05-02 | 1981-05-02 | Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. |
DK196282A DK155967C (da) | 1981-05-02 | 1982-04-30 | Fremgangsmaade samt kit til bestemmelse af antistoffer mod oploeselige antigener |
DE8282200517T DE3265768D1 (en) | 1981-05-02 | 1982-04-30 | A method and a kit for the assay of antibodies to soluble antigens |
US06/373,644 US4468470A (en) | 1981-05-02 | 1982-04-30 | Method and a kit for the assay of antibodies to soluble antigens |
CA000402054A CA1177391A (en) | 1981-05-02 | 1982-04-30 | Method and a kit for the assay of antibodies to soluable antigens |
IE1027/82A IE53059B1 (en) | 1981-05-02 | 1982-04-30 | A method and a kit for the assay of antibodies to soluble antigens |
ES512498A ES512498A0 (es) | 1981-05-02 | 1982-04-30 | "metodo y su correspondiente aparato para el ensayo de anticuerpos con respecto a antigenos solubles". |
AU83174/82A AU8317482A (en) | 1981-05-02 | 1982-04-30 | Assay of antibodies to soluble antigens |
AT82200517T ATE15274T1 (de) | 1981-05-02 | 1982-04-30 | Verfahren und ausruestung zum testen von antikoerpern gegen loesliche antigene. |
NO821431A NO160474C (no) | 1981-05-02 | 1982-04-30 | Fremgangsmaate og sett for paavisning og bestemmelse av antistoffer mot opploeselige antigener. |
JP57071591A JPS589071A (ja) | 1981-05-02 | 1982-04-30 | 抗体検定方法および抗体検定用試薬 |
EP82200517A EP0064318B1 (en) | 1981-05-02 | 1982-04-30 | A method and a kit for the assay of antibodies to soluble antigens |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL8102178A NL8102178A (nl) | 1981-05-02 | 1981-05-02 | Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. |
NL8102178 | 1981-05-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
NL8102178A true NL8102178A (nl) | 1982-12-01 |
Family
ID=19837436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NL8102178A NL8102178A (nl) | 1981-05-02 | 1981-05-02 | Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. |
Country Status (12)
Country | Link |
---|---|
US (1) | US4468470A (da) |
EP (1) | EP0064318B1 (da) |
JP (1) | JPS589071A (da) |
AT (1) | ATE15274T1 (da) |
AU (1) | AU8317482A (da) |
CA (1) | CA1177391A (da) |
DE (1) | DE3265768D1 (da) |
DK (1) | DK155967C (da) |
ES (1) | ES512498A0 (da) |
IE (1) | IE53059B1 (da) |
NL (1) | NL8102178A (da) |
NO (1) | NO160474C (da) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3225027A1 (de) * | 1982-07-05 | 1984-01-05 | Boehringer Mannheim Gmbh, 6800 Mannheim | Immunchemisches messverfahren |
GB8422452D0 (en) * | 1984-09-05 | 1984-10-10 | Serono Diagnostics Ltd | Assay |
US4806311A (en) * | 1985-08-28 | 1989-02-21 | Miles Inc. | Multizone analytical element having labeled reagent concentration zone |
US5262334A (en) * | 1986-01-30 | 1993-11-16 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
US5225353A (en) * | 1986-01-30 | 1993-07-06 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
JP2505179B2 (ja) * | 1986-12-16 | 1996-06-05 | 日本碍子株式会社 | 高強度常圧焼結窒化珪素焼結体およびその製造方法 |
US4859612A (en) * | 1987-10-07 | 1989-08-22 | Hygeia Sciences, Inc. | Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite |
US5202267A (en) * | 1988-04-04 | 1993-04-13 | Hygeia Sciences, Inc. | Sol capture immunoassay kit and procedure |
DE4140142A1 (de) * | 1991-12-05 | 1993-06-09 | Boehringer Mannheim Gmbh, 6800 Mannheim, De | Multivalentes dextranreagenz zum einsatz in praezipitationstests |
US5518882A (en) * | 1993-12-21 | 1996-05-21 | Biotex Laboratories, Inc. | Immunological methods of component selection and recovery |
FR2716263B1 (fr) * | 1994-02-11 | 1997-01-17 | Pasteur Institut | Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon. |
DE4434093A1 (de) * | 1994-09-23 | 1996-03-28 | Boehringer Mannheim Gmbh | Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz |
US5773224A (en) * | 1996-02-12 | 1998-06-30 | Grandics; Peter | Immunoselection system for cell elution |
US6436722B1 (en) | 2000-04-18 | 2002-08-20 | Idexx Laboratories, Inc. | Device and method for integrated diagnostics with multiple independent flow paths |
EP1195606A1 (en) * | 2000-10-03 | 2002-04-10 | VBC-Genomics Forschungsges.m.b.H. | Allergen-microarray assay |
CA2434139C (en) * | 2001-01-23 | 2014-05-27 | President And Fellows Of Harvard College | Nucleic-acid programmable protein arrays |
US7741050B2 (en) * | 2002-11-29 | 2010-06-22 | Max-Delbrueck-Centrum fuer Molkulare Medizin | Identification of agonistic autoantibodies |
DE10327066A1 (de) * | 2002-11-29 | 2004-09-16 | Max-Delbrück-Centrum für Molekulare Medizin | Bestimmung agonistischer Autoantikörper |
CA2563168A1 (en) * | 2004-04-14 | 2005-11-17 | President And Fellows Of Harvard College | Nucleic-acid programmable protein arrays |
US8178316B2 (en) * | 2006-06-29 | 2012-05-15 | President And Fellows Of Harvard College | Evaluating proteins |
RU2734958C2 (ru) | 2015-11-18 | 2020-10-26 | Формикон Аг | Предварительно заполненная фармацевтическая упаковка, содержащая жидкий состав на основе антагониста vegf |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE341239B (da) * | 1967-09-06 | 1971-12-20 | Pharmacia Ab | |
US3773625A (en) * | 1971-04-08 | 1973-11-20 | Us Army | Soluble antigen-antibody complexes |
US3966898A (en) * | 1973-06-28 | 1976-06-29 | Pharmacia Diagnostics Ab | Method and reagent for determining immunologic materials |
US4230797A (en) * | 1975-04-28 | 1980-10-28 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a coenzyme as label |
US4021534A (en) * | 1975-12-12 | 1977-05-03 | Hoffmann-La Roche Inc. | Radioimmunoassay |
SE427505B (sv) * | 1977-03-04 | 1983-04-11 | Pharmacia Diagnostics Ab | Reagens till anvendning vid immunkemiska bestemningsmetoder |
IL51667A (en) * | 1977-03-16 | 1979-10-31 | Miles Yeda Ltd | Immunoassay for the determination of a hapten |
US4271140A (en) * | 1978-01-23 | 1981-06-02 | Baxter Travenol Laboratories, Inc. | Method and composition for double receptor, specific binding assays |
JPS5510590A (en) * | 1978-05-04 | 1980-01-25 | Wellcome Found | Enzyme immunity quantity analysis |
US4228237A (en) * | 1978-09-21 | 1980-10-14 | Calbiochem-Behring Corp. | Methods for the detection and determination of ligands |
US4495296A (en) * | 1979-05-21 | 1985-01-22 | New York Blood Center, Inc. | Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays |
-
1981
- 1981-05-02 NL NL8102178A patent/NL8102178A/nl not_active Application Discontinuation
-
1982
- 1982-04-30 CA CA000402054A patent/CA1177391A/en not_active Expired
- 1982-04-30 IE IE1027/82A patent/IE53059B1/en unknown
- 1982-04-30 EP EP82200517A patent/EP0064318B1/en not_active Expired
- 1982-04-30 US US06/373,644 patent/US4468470A/en not_active Expired - Lifetime
- 1982-04-30 DK DK196282A patent/DK155967C/da not_active IP Right Cessation
- 1982-04-30 JP JP57071591A patent/JPS589071A/ja active Pending
- 1982-04-30 DE DE8282200517T patent/DE3265768D1/de not_active Expired
- 1982-04-30 AU AU83174/82A patent/AU8317482A/en not_active Abandoned
- 1982-04-30 ES ES512498A patent/ES512498A0/es active Granted
- 1982-04-30 NO NO821431A patent/NO160474C/no unknown
- 1982-04-30 AT AT82200517T patent/ATE15274T1/de active
Also Published As
Publication number | Publication date |
---|---|
NO160474C (no) | 1989-04-19 |
EP0064318A1 (en) | 1982-11-10 |
DE3265768D1 (en) | 1985-10-03 |
NO821431L (no) | 1982-11-03 |
EP0064318B1 (en) | 1985-08-28 |
DK155967C (da) | 1989-10-16 |
ES8304314A1 (es) | 1983-02-16 |
IE53059B1 (en) | 1988-05-25 |
NO160474B (no) | 1989-01-09 |
ATE15274T1 (de) | 1985-09-15 |
ES512498A0 (es) | 1983-02-16 |
DK196282A (da) | 1982-11-03 |
DK155967B (da) | 1989-06-05 |
JPS589071A (ja) | 1983-01-19 |
AU8317482A (en) | 1982-11-11 |
CA1177391A (en) | 1984-11-06 |
US4468470A (en) | 1984-08-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
A1B | A search report has been drawn up | ||
A85 | Still pending on 85-01-01 | ||
BC | A request for examination has been filed | ||
BI | The patent application has been withdrawn |