MXPA03000435A - Variantes de enzimas que degradan la pared celular. - Google Patents

Variantes de enzimas que degradan la pared celular.

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Publication number
MXPA03000435A
MXPA03000435A MXPA03000435A MXPA03000435A MXPA03000435A MX PA03000435 A MXPA03000435 A MX PA03000435A MX PA03000435 A MXPA03000435 A MX PA03000435A MX PA03000435 A MXPA03000435 A MX PA03000435A MX PA03000435 A MXPA03000435 A MX PA03000435A
Authority
MX
Mexico
Prior art keywords
stack
variant
amino acids
hydrophobic
residues
Prior art date
Application number
MXPA03000435A
Other languages
English (en)
Inventor
Carsten Andersen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MXPA03000435A publication Critical patent/MXPA03000435A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Abstract

Una variante de una enzima que degrada la pared celular que tiene una estructura de helice ¦, variante la cual contiene al menos un sustituyente en una posicion determinada mediante la identificacion de todos los residuos potencialmente pertenecientes a un bloque; caracterizando el bloque como interior o exterior; caracterizando el bloque como polar, hidrofobico o aromatico/heteroaromatico sobre la base de las caracteristicas dominantes de los residuos del bloque de la enzima original o natural y/o su orientacion en relacion a la helice ¦ (interior o exterior); optimizando todas las posiciones del bloque de un bloque, ya sea a aminoacidos alifaticos hidrofobicos, aminoacidos aromaticos hidrofobicos o polares, permitiendo mutaciones dentro de una o todas las posiciones a aminoacidos pertenecientes a uno de esos grupos; midiendo la termoestabilidad de las variantes por DSC o un ensayo relacionado con la aplicacion como la prueba de aplicacion Pad-Steam; y seleccionando las variantes estabilizadas. La variante de una pectato liasa original, natural (EC 4.2.2.2) que tiene los aminoacidos residuales conservados D111, D141 o E141, D145, K165, R194 y R199, cuando se alinea con la pectato liasa que comprende la secuencia de aminoacidos de la SEQ ID NO: 2 es la preferida.
MXPA03000435A 2000-07-19 2001-07-17 Variantes de enzimas que degradan la pared celular. MXPA03000435A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200001117 2000-07-19
DKPA200100705 2001-05-04
DKPA200100734 2001-05-10
PCT/DK2001/000505 WO2002006442A2 (en) 2000-07-19 2001-07-17 Cell-wall degrading enzyme variants

Publications (1)

Publication Number Publication Date
MXPA03000435A true MXPA03000435A (es) 2004-04-02

Family

ID=27222417

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA03000435A MXPA03000435A (es) 2000-07-19 2001-07-17 Variantes de enzimas que degradan la pared celular.

Country Status (8)

Country Link
EP (1) EP1305408B1 (es)
CN (1) CN1230530C (es)
AR (1) AR033986A1 (es)
AT (1) ATE421579T1 (es)
AU (1) AU2001281736A1 (es)
DE (1) DE60137510D1 (es)
MX (1) MXPA03000435A (es)
WO (1) WO2002006442A2 (es)

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Also Published As

Publication number Publication date
WO2002006442A2 (en) 2002-01-24
AU2001281736A1 (en) 2002-01-30
ATE421579T1 (de) 2009-02-15
CN1230530C (zh) 2005-12-07
AR033986A1 (es) 2004-01-21
CN1443237A (zh) 2003-09-17
EP1305408A2 (en) 2003-05-02
DE60137510D1 (de) 2009-03-12
WO2002006442A3 (en) 2002-09-26
EP1305408B1 (en) 2009-01-21

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