MXPA02011150A - Manipulacion del canal microfluido del embrion y/o el oocito, analisis y evaluacion biologica. - Google Patents

Manipulacion del canal microfluido del embrion y/o el oocito, analisis y evaluacion biologica.

Info

Publication number
MXPA02011150A
MXPA02011150A MXPA02011150A MXPA02011150A MXPA02011150A MX PA02011150 A MXPA02011150 A MX PA02011150A MX PA02011150 A MXPA02011150 A MX PA02011150A MX PA02011150 A MXPA02011150 A MX PA02011150A MX PA02011150 A MXPA02011150 A MX PA02011150A
Authority
MX
Mexico
Prior art keywords
embryos
microfluidic
embryo
channel
opportunity
Prior art date
Application number
MXPA02011150A
Other languages
English (en)
Inventor
Matthew B Wheeler
Original Assignee
Univ Illinois
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/570,483 external-priority patent/US6695765B1/en
Application filed by Univ Illinois filed Critical Univ Illinois
Publication of MXPA02011150A publication Critical patent/MXPA02011150A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Abstract

Los canales escalados de embriones de microfluidos para el manejo y posicionamiento de embriones proporcionan la oportunidad de evaluar y tratar los embriones en maneras mejoradas. El flujo del fluido se usa para mover y colocar los embriones dentro de los canales de microfluidos y las geometrias del canal pueden usarse para colocar los embriones en ubicaciones especificas. Las propiedades de la superficie y las propiedades de conformidad (deformacion) de los embriones se evaluan como un pronosticador de viabilidad. Los canales de microfluido proporcionan la oportunidad de controles finos de presion para conducir las evaluaciones en fuerzas ligeramente abajo, las cuales danan a los embriones y que se conoce que ocurre. La medicion de la distancia y/o que los embriones giran en un mismo canal de microfluido de gradiente de presion proporciona informacion con embriones saludables que viajan mas despacio o a distancia mas corta a medida que demuestran mas friccion estatica a las paredes del canal. El posicionamiento en un estrechamiento (14a, 14b, 24, 26), los embriones saludables tambien aparecen para deformarlos menos que a los embriones saludables que se arrastran mas rapidamente dentro de un estrangulamiento. Ademas, los embriones saludables aparecen para resumir su mejor forma. El fluido de los canales de microfluido se colecta mas facilmente en forma descendente sin alterar las tecnicas de muestreo del embrion, proporcionando una mejor oportunidad para el analisis quimico del analisis quimico del fluido que las tecnicas de muestreo y manejo manual convencional. La remocion de la zona diafana de los embriones de mamiferos se logra a medida que los embriones de mueven a traves del flujo a una ubicacion precisa en donde el agente de lisado puede lavarse sobre el embrion para alcanzar la zona diafana. La remocion del cumulo se realiza con una serie de estrechamientos para cortar el cumulo seguido por el flujo del fluido para remover el corte del cumulo del embrion.
MXPA02011150A 2000-05-12 2001-05-08 Manipulacion del canal microfluido del embrion y/o el oocito, analisis y evaluacion biologica. MXPA02011150A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/570,483 US6695765B1 (en) 1999-04-08 2000-05-12 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
PCT/US2001/014822 WO2001088087A2 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Publications (1)

Publication Number Publication Date
MXPA02011150A true MXPA02011150A (es) 2004-08-19

Family

ID=24279818

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02011150A MXPA02011150A (es) 2000-05-12 2001-05-08 Manipulacion del canal microfluido del embrion y/o el oocito, analisis y evaluacion biologica.

Country Status (11)

Country Link
EP (1) EP1280465A4 (es)
JP (1) JP2003533221A (es)
KR (1) KR20030009482A (es)
CN (1) CN1441652A (es)
AU (2) AU5963501A (es)
BR (1) BR0110768A (es)
CA (1) CA2408616C (es)
IL (1) IL152650A (es)
MX (1) MXPA02011150A (es)
RU (1) RU2002130577A (es)
WO (1) WO2001088087A2 (es)

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EP1641411A1 (en) * 2003-06-06 2006-04-05 The Regents Of The University Of Michigan Integrated microfluidic sperm isolation and insemination device
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1609850A1 (en) 2004-06-24 2005-12-28 Biovir v/Jacob Mollenbach Culture dish for culturing biological cells
JP2006055072A (ja) * 2004-08-20 2006-03-02 Fujitsu Ltd 搬送制御装置及び方法
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US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
JP2010536348A (ja) * 2007-08-24 2010-12-02 スマート・バイオシステムズ・エーピーエス 灌流のための中規模バイオリアクタープラットフォーム
TW200920841A (en) * 2007-09-25 2009-05-16 Cytyc Corp Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen
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WO2009079474A1 (en) * 2007-12-14 2009-06-25 The University Of North Carolina At Chapel Hill Methods, systems, and computer readable media for facilitating automation of blastocyst microinjection
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CN112154031B (zh) * 2018-04-04 2023-05-23 综合医院公司 用于剥离哺乳动物卵母细胞的微流体系统和方法
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Also Published As

Publication number Publication date
CA2408616A1 (en) 2001-11-22
KR20030009482A (ko) 2003-01-29
EP1280465A2 (en) 2003-02-05
CN1441652A (zh) 2003-09-10
RU2002130577A (ru) 2004-03-27
AU2001259635B2 (en) 2005-05-19
EP1280465A4 (en) 2004-10-20
BR0110768A (pt) 2003-03-11
WO2001088087A3 (en) 2002-03-28
WO2001088087A2 (en) 2001-11-22
AU5963501A (en) 2001-11-26
CA2408616C (en) 2007-10-02
JP2003533221A (ja) 2003-11-11
IL152650A0 (en) 2003-06-24
IL152650A (en) 2006-12-31

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