CA2408616C - Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation - Google Patents

Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation Download PDF

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Publication number
CA2408616C
CA2408616C CA002408616A CA2408616A CA2408616C CA 2408616 C CA2408616 C CA 2408616C CA 002408616 A CA002408616 A CA 002408616A CA 2408616 A CA2408616 A CA 2408616A CA 2408616 C CA2408616 C CA 2408616C
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CA
Canada
Prior art keywords
embryo
embryos
fluid
channel
fluid channel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002408616A
Other languages
English (en)
French (fr)
Other versions
CA2408616A1 (en
Inventor
David J. Beebe
Ian K. Glasgow
Matthew B. Wheeler
Henry Zeringue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Illinois
Original Assignee
University of Illinois
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/570,483 external-priority patent/US6695765B1/en
Application filed by University of Illinois filed Critical University of Illinois
Publication of CA2408616A1 publication Critical patent/CA2408616A1/en
Application granted granted Critical
Publication of CA2408616C publication Critical patent/CA2408616C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Fluid Mechanics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA002408616A 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation Expired - Fee Related CA2408616C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/570,483 2000-05-12
US09/570,483 US6695765B1 (en) 1999-04-08 2000-05-12 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
PCT/US2001/014822 WO2001088087A2 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Publications (2)

Publication Number Publication Date
CA2408616A1 CA2408616A1 (en) 2001-11-22
CA2408616C true CA2408616C (en) 2007-10-02

Family

ID=24279818

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002408616A Expired - Fee Related CA2408616C (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Country Status (11)

Country Link
EP (1) EP1280465A4 (es)
JP (1) JP2003533221A (es)
KR (1) KR20030009482A (es)
CN (1) CN1441652A (es)
AU (2) AU2001259635B2 (es)
BR (1) BR0110768A (es)
CA (1) CA2408616C (es)
IL (1) IL152650A (es)
MX (1) MXPA02011150A (es)
RU (1) RU2002130577A (es)
WO (1) WO2001088087A2 (es)

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AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
WO2004101743A2 (en) * 2003-05-06 2004-11-25 Bellbrook Labs, Llc Three dimensional cell cultures in a microscale fluid handling system
CA2527733A1 (en) * 2003-06-06 2004-12-16 The Regents Of The University Of Michigan Integrated microfluidic sperm isolation and insemination device
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1609850A1 (en) 2004-06-24 2005-12-28 Biovir v/Jacob Mollenbach Culture dish for culturing biological cells
JP2006055072A (ja) * 2004-08-20 2006-03-02 Fujitsu Ltd 搬送制御装置及び方法
CA2601318A1 (en) * 2005-03-18 2006-09-28 Effector Cell Institute, Inc. Cell observation aiding instrument and method of cell observation therewith
GB0508983D0 (en) * 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
US20070090166A1 (en) * 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
CN101835886A (zh) * 2007-08-24 2010-09-15 智能生物系统有限公司 用于灌注的中等尺寸生物反应器平台
TW200920841A (en) * 2007-09-25 2009-05-16 Cytyc Corp Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen
KR100891487B1 (ko) * 2007-11-01 2009-04-01 한국과학기술원 미세유체제어기술 기반으로 한 난모세포 또는 난자의 핵제거 시스템
WO2009079474A1 (en) * 2007-12-14 2009-06-25 The University Of North Carolina At Chapel Hill Methods, systems, and computer readable media for facilitating automation of blastocyst microinjection
US20110236970A1 (en) * 2008-08-01 2011-09-29 Smart Biosystems Aps Chamber of a bioreactor platform
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
GB0821636D0 (en) 2008-11-26 2008-12-31 Ucl Business Plc Device
US20120196358A1 (en) * 2009-06-01 2012-08-02 Fred Burbank Device for removing cumulus from oocytes
US8621943B2 (en) * 2009-09-30 2014-01-07 Weyerhaeuser Nr Company Method of singulating embryos
EP2316565A1 (en) * 2009-10-26 2011-05-04 Fei Company A micro-reactor for observing particles in a fluid
CN101947124B (zh) * 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
CN103517676A (zh) * 2010-09-27 2014-01-15 奥克索金股份有限公司 用于胚胎、卵母细胞和干细胞的自动化成像和评价的装置、方法和系统
ES2725825T3 (es) * 2012-04-16 2019-09-27 Univ Cornell Sistema de fertilización asistida por inyección de esperma intracitoplasmática automatizada
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US10662408B2 (en) 2013-03-14 2020-05-26 Inguran, Llc Methods for high throughput sperm sorting
AU2015203738B2 (en) * 2013-03-14 2017-10-05 Inguran, Llc Apparatus and methods for high throughput sperm sorting
US10371622B2 (en) 2013-03-14 2019-08-06 Inguran, Llc Device for high throughput sperm sorting
AU2013202632B2 (en) * 2013-03-14 2015-04-16 Inguran, Llc Apparatus and methods for high throughput sperm sorting
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
JP2020510417A (ja) 2017-02-17 2020-04-09 コニク インコーポレイテッド 検出のためのシステム
KR101959208B1 (ko) * 2017-05-24 2019-03-19 한국기계연구원 액적기반 바이오칩, 이를 이용한 종양 생산방법, 및 이를 통해 생산된 종양의 전이성 판단방법
CA3096048A1 (en) * 2018-04-04 2019-10-10 The General Hospital Corporation Microfluidic systems and methods to denude mammalian oocytes
US11654434B2 (en) 2018-06-24 2023-05-23 Nemametrix Inc. Microinjection chip, device, system and uses thereof for unicellular or multicellular organisms
FR3084121B1 (fr) * 2018-07-17 2021-01-15 Skf Aerospace France Assemblage mecanique et dispositif mecanique
CN111073817A (zh) * 2018-10-22 2020-04-28 曾繁根 用于精子分选、卵子培育、体外受精的整合式晶片及方法
CN111378556A (zh) * 2018-12-29 2020-07-07 青岛华大智造普惠科技有限公司 微流控芯片及其制备方法、单细胞微液滴的制备方法
CN114340694A (zh) * 2019-05-24 2022-04-12 美国休士顿大学系统 用于激光驱动型微流体泵的医疗设备的装置和方法
KR102267433B1 (ko) * 2019-07-29 2021-06-21 연세대학교 산학협력단 세포배양 플레이트
KR102243812B1 (ko) * 2019-08-13 2021-04-22 경희대학교 산학협력단 체외수정 자동화 장치 및 그것의 동작 방법
DE102020214862B4 (de) * 2020-11-26 2022-08-04 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Körperschaft des öffentlichen Rechts System und Vorrichtung zur Charakterisierung von Veränderungen des Reifungsvorgangs einer Eizelle, sowie deren Verwendung
CN117660183B (zh) * 2024-01-31 2024-04-16 山东大学 胚胎微流控动态培养皿及其培养方法

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JP3298882B2 (ja) * 1992-05-01 2002-07-08 トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア 微細加工した検出構造体
CA2333201A1 (en) * 1998-05-22 1999-12-02 California Institute Of Technology Microfabricated cell sorter
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method

Also Published As

Publication number Publication date
BR0110768A (pt) 2003-03-11
IL152650A (en) 2006-12-31
CN1441652A (zh) 2003-09-10
MXPA02011150A (es) 2004-08-19
KR20030009482A (ko) 2003-01-29
CA2408616A1 (en) 2001-11-22
EP1280465A2 (en) 2003-02-05
JP2003533221A (ja) 2003-11-11
AU5963501A (en) 2001-11-26
IL152650A0 (en) 2003-06-24
EP1280465A4 (en) 2004-10-20
WO2001088087A2 (en) 2001-11-22
RU2002130577A (ru) 2004-03-27
WO2001088087A3 (en) 2002-03-28
AU2001259635B2 (en) 2005-05-19

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