CA2408616A1 - Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation - Google Patents

Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation Download PDF

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Publication number
CA2408616A1
CA2408616A1 CA002408616A CA2408616A CA2408616A1 CA 2408616 A1 CA2408616 A1 CA 2408616A1 CA 002408616 A CA002408616 A CA 002408616A CA 2408616 A CA2408616 A CA 2408616A CA 2408616 A1 CA2408616 A1 CA 2408616A1
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CA
Canada
Prior art keywords
embryos
microfluidic
embryo
channel
opportunity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002408616A
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French (fr)
Other versions
CA2408616C (en
Inventor
David J. Beebe
Ian K. Glasgow
Matthew B. Wheeler
Henry Zeringue
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University of Illinois
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Individual
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Filing date
Publication date
Priority claimed from US09/570,483 external-priority patent/US6695765B1/en
Application filed by Individual filed Critical Individual
Publication of CA2408616A1 publication Critical patent/CA2408616A1/en
Application granted granted Critical
Publication of CA2408616C publication Critical patent/CA2408616C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners.
Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations.
Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and/or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls.
CA002408616A 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation Expired - Fee Related CA2408616C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/570,483 2000-05-12
US09/570,483 US6695765B1 (en) 1999-04-08 2000-05-12 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
PCT/US2001/014822 WO2001088087A2 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Publications (2)

Publication Number Publication Date
CA2408616A1 true CA2408616A1 (en) 2001-11-22
CA2408616C CA2408616C (en) 2007-10-02

Family

ID=24279818

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002408616A Expired - Fee Related CA2408616C (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Country Status (11)

Country Link
EP (1) EP1280465A4 (en)
JP (1) JP2003533221A (en)
KR (1) KR20030009482A (en)
CN (1) CN1441652A (en)
AU (2) AU5963501A (en)
BR (1) BR0110768A (en)
CA (1) CA2408616C (en)
IL (1) IL152650A (en)
MX (1) MXPA02011150A (en)
RU (1) RU2002130577A (en)
WO (1) WO2001088087A2 (en)

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WO2003085379A2 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
WO2004101743A2 (en) * 2003-05-06 2004-11-25 Bellbrook Labs, Llc Three dimensional cell cultures in a microscale fluid handling system
EP1641411A1 (en) * 2003-06-06 2006-04-05 The Regents Of The University Of Michigan Integrated microfluidic sperm isolation and insemination device
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1609850A1 (en) 2004-06-24 2005-12-28 Biovir v/Jacob Mollenbach Culture dish for culturing biological cells
JP2006055072A (en) * 2004-08-20 2006-03-02 Fujitsu Ltd Apparatus and method for conveying control
US20090004730A1 (en) * 2005-03-18 2009-01-01 Nao Nitta Cell Obsevation Aiding Instrument and Method of Cell Observation Therewith
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
US20070090166A1 (en) * 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
WO2009026931A2 (en) * 2007-08-24 2009-03-05 Smart Biosystems Aps Mesoscale bioreactor platform for perfusion
TW200920841A (en) * 2007-09-25 2009-05-16 Cytyc Corp Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen
KR100891487B1 (en) * 2007-11-01 2009-04-01 한국과학기술원 Enucleation system of oocyte or ovum which based on microfluid mechanics
WO2009079474A1 (en) * 2007-12-14 2009-06-25 The University Of North Carolina At Chapel Hill Methods, systems, and computer readable media for facilitating automation of blastocyst microinjection
EP2318505A1 (en) * 2008-08-01 2011-05-11 Smart Biosystems APS A chamber of a bioreactor platform
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
GB0821636D0 (en) 2008-11-26 2008-12-31 Ucl Business Plc Device
WO2010141320A1 (en) * 2009-06-01 2010-12-09 Mariposa Biotechnology, Inc. Device for removing cumulus from oocytes
US8621943B2 (en) * 2009-09-30 2014-01-07 Weyerhaeuser Nr Company Method of singulating embryos
EP2316565A1 (en) * 2009-10-26 2011-05-04 Fei Company A micro-reactor for observing particles in a fluid
CN101947124B (en) 2010-06-25 2012-07-04 博奥生物有限公司 Integrated microfluidic chip device and using method thereof
US9482659B2 (en) * 2010-09-27 2016-11-01 Progyny, Inc. Apparatus, method, and system for the automated imaging and evaluation of embryos, oocytes and stem cells
EP2838987B1 (en) * 2012-04-16 2019-03-06 Cornell University Automated intracytoplasmic sperm injection assisted fertilization system
US10662408B2 (en) 2013-03-14 2020-05-26 Inguran, Llc Methods for high throughput sperm sorting
AU2015203738B2 (en) * 2013-03-14 2017-10-05 Inguran, Llc Apparatus and methods for high throughput sperm sorting
AU2013202635B2 (en) * 2013-03-14 2015-10-29 Inguran, Llc Apparatus and methods for high throughput sperm sorting
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US10371622B2 (en) 2013-03-14 2019-08-06 Inguran, Llc Device for high throughput sperm sorting
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US11474103B2 (en) 2017-02-17 2022-10-18 Koniku Inc. Systems for detection
KR101959208B1 (en) * 2017-05-24 2019-03-19 한국기계연구원 Droplet based biochip, a method for producing a tumor, and a method for deciding a metastasis tumor
CN112154031B (en) * 2018-04-04 2023-05-23 综合医院公司 Microfluidic system and method for dissecting mammalian oocytes
WO2020005802A1 (en) * 2018-06-24 2020-01-02 University Of Oregon Microinjection chip, device, system and uses thereof for unicellular or multicellular organisms
FR3084121B1 (en) * 2018-07-17 2021-01-15 Skf Aerospace France MECHANICAL ASSEMBLY AND MECHANICAL DEVICE
CN111073817A (en) * 2018-10-22 2020-04-28 曾繁根 Integrated wafer and method for sperm sorting, ovum breeding, in vitro fertilization
CN111378556A (en) * 2018-12-29 2020-07-07 青岛华大智造普惠科技有限公司 Micro-fluidic chip and preparation method thereof, and preparation method of single-cell micro-droplets
US20220218896A1 (en) * 2019-05-24 2022-07-14 University Of Houston System Apparatus and methods for medical applications of laser driven microfluid pumps
KR102267433B1 (en) * 2019-07-29 2021-06-21 연세대학교 산학협력단 A cell culture plate
KR102243812B1 (en) * 2019-08-13 2021-04-22 경희대학교 산학협력단 Automated in vitro fertilization device and method for operating thereof
DE102020214862B4 (en) * 2020-11-26 2022-08-04 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Körperschaft des öffentlichen Rechts System and device for characterizing changes in the maturation process of an egg cell, and their use
WO2024208924A1 (en) * 2023-04-03 2024-10-10 College De France Microfluidic device for use in a system for measuring at least one mechanical property of at least one oocyte
CN117660183B (en) * 2024-01-31 2024-04-16 山东大学 Embryo microfluidic dynamic culture dish and culture method thereof

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WO1993022058A1 (en) * 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Polynucleotide amplification analysis using a microfabricated device
ATE530891T1 (en) * 1998-05-22 2011-11-15 California Inst Of Techn MINIATURIZED CELL SORTER
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method

Also Published As

Publication number Publication date
EP1280465A4 (en) 2004-10-20
IL152650A0 (en) 2003-06-24
KR20030009482A (en) 2003-01-29
BR0110768A (en) 2003-03-11
AU2001259635B2 (en) 2005-05-19
CN1441652A (en) 2003-09-10
JP2003533221A (en) 2003-11-11
WO2001088087A2 (en) 2001-11-22
AU5963501A (en) 2001-11-26
IL152650A (en) 2006-12-31
WO2001088087A3 (en) 2002-03-28
CA2408616C (en) 2007-10-02
RU2002130577A (en) 2004-03-27
MXPA02011150A (en) 2004-08-19
EP1280465A2 (en) 2003-02-05

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Effective date: 20140508