WO2004101743A3 - Three dimensional cell cultures in a microscale fluid handling system - Google Patents

Three dimensional cell cultures in a microscale fluid handling system Download PDF

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Publication number
WO2004101743A3
WO2004101743A3 PCT/US2004/014092 US2004014092W WO2004101743A3 WO 2004101743 A3 WO2004101743 A3 WO 2004101743A3 US 2004014092 W US2004014092 W US 2004014092W WO 2004101743 A3 WO2004101743 A3 WO 2004101743A3
Authority
WO
WIPO (PCT)
Prior art keywords
handling system
fluid handling
cell cultures
dimensional cell
microscale fluid
Prior art date
Application number
PCT/US2004/014092
Other languages
French (fr)
Other versions
WO2004101743A2 (en
Inventor
Robert G Lowery
John Majer
David J Beebe
Original Assignee
Bellbrook Labs Llc
Robert G Lowery
John Majer
David J Beebe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bellbrook Labs Llc, Robert G Lowery, John Majer, David J Beebe filed Critical Bellbrook Labs Llc
Priority to CA002524496A priority Critical patent/CA2524496A1/en
Priority to EP04760894A priority patent/EP1636332A4/en
Priority to JP2006532811A priority patent/JP2007501633A/en
Publication of WO2004101743A2 publication Critical patent/WO2004101743A2/en
Publication of WO2004101743A3 publication Critical patent/WO2004101743A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes

Abstract

The present invention provides a novel microscale fluid handling system for initiating, culturing, manipulating and assaying three-dimensional multicellular assembly. The system including a microfluidic device and three-dimensional multicellular tissue surrogate assembly. The device of the invention includes at least one microfluidic channel; and at least one chamber, wherein the walls of the chamber are lined with a cell layer; and wherein fluid medium flows through each of the channels and chambers. Also, disclosed are methods for using the device to introducing test agents to the multicellular assemblies to observe biological responses thereof.
PCT/US2004/014092 2003-05-06 2004-05-06 Three dimensional cell cultures in a microscale fluid handling system WO2004101743A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002524496A CA2524496A1 (en) 2003-05-06 2004-05-06 Three dimensional cell cultures in a microscale fluid handling system
EP04760894A EP1636332A4 (en) 2003-05-06 2004-05-06 Three dimensional cell cultures in a microscale fluid handling system
JP2006532811A JP2007501633A (en) 2003-05-06 2004-05-06 Three-dimensional cell culture in a microscale fluid handling system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46835803P 2003-05-06 2003-05-06
US60/468,358 2003-05-06

Publications (2)

Publication Number Publication Date
WO2004101743A2 WO2004101743A2 (en) 2004-11-25
WO2004101743A3 true WO2004101743A3 (en) 2005-06-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/014092 WO2004101743A2 (en) 2003-05-06 2004-05-06 Three dimensional cell cultures in a microscale fluid handling system

Country Status (5)

Country Link
US (1) US20040259177A1 (en)
EP (1) EP1636332A4 (en)
JP (1) JP2007501633A (en)
CA (1) CA2524496A1 (en)
WO (1) WO2004101743A2 (en)

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WO2013116729A1 (en) * 2012-02-02 2013-08-08 The Trustees Of The University Of Pennsylvania Microfabricated 3d cell culture system
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Also Published As

Publication number Publication date
EP1636332A4 (en) 2007-08-29
WO2004101743A2 (en) 2004-11-25
JP2007501633A (en) 2007-02-01
EP1636332A2 (en) 2006-03-22
CA2524496A1 (en) 2004-11-25
US20040259177A1 (en) 2004-12-23

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