BRPI0001536A - método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp - Google Patents

método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp

Info

Publication number
BRPI0001536A
BRPI0001536A BRPI0001536A BRPI0001536A BRPI0001536A BR PI0001536 A BRPI0001536 A BR PI0001536A BR PI0001536 A BRPI0001536 A BR PI0001536A BR PI0001536 A BRPI0001536 A BR PI0001536A BR PI0001536 A BRPI0001536 A BR PI0001536A
Authority
BR
Brazil
Prior art keywords
schistosoma
diagnosing
infection
kit
parasite
Prior art date
Application number
BRPI0001536A
Other languages
English (en)
Inventor
Rabello Ana Lucia Teles
Neto Emmanuel Dias
Luis Andre Pontes
Original Assignee
Fundacao Oswaldo Cruz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao Oswaldo Cruz filed Critical Fundacao Oswaldo Cruz
Priority to BRPI0001536A priority Critical patent/BRPI0001536B1/pt
Priority to AU43971/01A priority patent/AU4397101A/en
Priority to EP01916775A priority patent/EP1208241B1/en
Priority to JP2001573022A priority patent/JP4974432B2/ja
Priority to ES01916775T priority patent/ES2237562T3/es
Priority to US09/980,277 priority patent/US6818402B2/en
Priority to CN01800817A priority patent/CN1366553A/zh
Priority to AT01916775T priority patent/ATE286984T1/de
Priority to DE60108337T priority patent/DE60108337T2/de
Priority to PCT/BR2001/000035 priority patent/WO2001075148A1/en
Publication of BRPI0001536A publication Critical patent/BRPI0001536A/pt
Publication of BRPI0001536B1 publication Critical patent/BRPI0001536B1/pt
Publication of BRPI0001536B8 publication Critical patent/BRPI0001536B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

"método e kit para a detecção da esquistossomose pela reação em cadeia da polimerase". o objetivo da presente invenção é a detecção de schistosoma sp. em amostras biológicas através de pcr. uma primeira concretização da presente invenção diz respeito a um método para diagnóstico da esquistossomose através da amplificação por pcr da sequência do dna de schistosoma sp., seguido de separação dos produtos de amplificação por eletroforese e da detecção por técnica apropriada. numa segunda concretização, a invenção é dirigida a kit de diagnóstico a ser utilizado na detecção da esquistossomose. um kit básico compreende todos os reagentes necessários para a realização da técnica de pcr, a saber: iniciadores específicos, nucleotídeos e solução tampão apropriada para a amplificação por pcr. opcionalmente, o kit pode conter a enzima taq polimerase em quantidades suficientes para amplificação, dna padrão para ser usado como controle positivo da reação, solução tampão da amostra para preparar o material amplificado para eletroforese e protocolo e manual para instruir o usuário.
BRPI0001536A 2000-04-04 2000-04-04 método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp BRPI0001536B1 (pt)

Priority Applications (10)

Application Number Priority Date Filing Date Title
BRPI0001536A BRPI0001536B1 (pt) 2000-04-04 2000-04-04 método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp
AU43971/01A AU4397101A (en) 2000-04-04 2001-04-04 Method and kit for the detection of schistosomiasis through the polymerase chainreaction
EP01916775A EP1208241B1 (en) 2000-04-04 2001-04-04 Method and kit for the detection of schistosomiasis through the polymerase chain reaction
JP2001573022A JP4974432B2 (ja) 2000-04-04 2001-04-04 Ｐｃｒ法によって住血吸虫症を検出するための方法及びそのキット
ES01916775T ES2237562T3 (es) 2000-04-04 2001-04-04 Procedimiento y kit para detectar la esquistosomiasis mediante la reaccion en cadena de la polimerasa.
US09/980,277 US6818402B2 (en) 2000-04-04 2001-04-04 Method and kit for the detection of schistosomiasis through the polymerase chain reaction
CN01800817A CN1366553A (zh) 2000-04-04 2001-04-04 通过聚合酶链式反应检测血吸虫病的方法和试剂盒
AT01916775T ATE286984T1 (de) 2000-04-04 2001-04-04 Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion
DE60108337T DE60108337T2 (de) 2000-04-04 2001-04-04 Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion
PCT/BR2001/000035 WO2001075148A1 (en) 2000-04-04 2001-04-04 Method and kit for the detection of schistosomiasis through the polymerase chain reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0001536A BRPI0001536B1 (pt) 2000-04-04 2000-04-04 método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp

Publications (3)

Publication Number Publication Date
BRPI0001536A true BRPI0001536A (pt) 2001-12-04
BRPI0001536B1 BRPI0001536B1 (pt) 2015-09-29
BRPI0001536B8 BRPI0001536B8 (pt) 2021-07-06

Family

ID=77336322

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0001536A BRPI0001536B1 (pt) 2000-04-04 2000-04-04 método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp

Country Status (10)

Country Link
US (1) US6818402B2 (pt)
EP (1) EP1208241B1 (pt)
JP (1) JP4974432B2 (pt)
CN (1) CN1366553A (pt)
AT (1) ATE286984T1 (pt)
AU (1) AU4397101A (pt)
BR (1) BRPI0001536B1 (pt)
DE (1) DE60108337T2 (pt)
ES (1) ES2237562T3 (pt)
WO (1) WO2001075148A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100383162C (zh) * 2005-05-10 2008-04-23 安徽医科大学 用于血吸虫病检测的组合检测试剂及检测方法
US7455844B2 (en) 2006-03-29 2008-11-25 Merial Limited Vaccine against streptococci
CN101457258B (zh) * 2008-12-26 2011-06-15 江苏省血吸虫病防治研究所 一种血吸虫感染性钉螺检测试剂盒及其检测方法
ES2541178T3 (es) 2009-02-03 2015-07-16 Mdxhealth Sa Método de detección de cáncer colorrectal
CN101597645B (zh) * 2009-06-26 2012-05-30 华南农业大学 一种日本血吸虫性别特异性检测引物、检测试剂盒和检测方法
CN102010861B (zh) * 2009-07-16 2014-02-26 上海人类基因组研究中心 日本血吸虫反转座子dna靶序列在血吸虫病诊断中的应用
RU2509156C1 (ru) * 2012-06-19 2014-03-10 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Способ видовой днк-дифференциации на разных стадиях жизненного цикла гельминтов-возбудителей церкариального дерматита человека
CN110760588A (zh) * 2019-07-18 2020-02-07 中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心) 一种用于日本血吸虫检测的核酸诊断试剂盒及其检测方法
CN110791576A (zh) * 2019-12-23 2020-02-14 江苏省血吸虫病防治研究所 检测曼氏血吸虫的引物组、探针及试剂盒
CN113151496A (zh) * 2021-04-20 2021-07-23 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心) Lfd-rpa可视化快速检测曼氏血吸虫核酸的引物、探针、试剂盒及方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005158A1 (en) * 1994-07-25 1997-02-13 The Board Of Trustees Of The Leland Stanford Junior University Compositions, treatments, and diagnostics for schistosomiasis and related diseases

Also Published As

Publication number Publication date
BRPI0001536B1 (pt) 2015-09-29
JP4974432B2 (ja) 2012-07-11
JP2003529373A (ja) 2003-10-07
BRPI0001536B8 (pt) 2021-07-06
US20030096244A1 (en) 2003-05-22
WO2001075148A1 (en) 2001-10-11
EP1208241B1 (en) 2005-01-12
AU4397101A (en) 2001-10-15
ES2237562T3 (es) 2005-08-01
CN1366553A (zh) 2002-08-28
EP1208241A1 (en) 2002-05-29
US6818402B2 (en) 2004-11-16
ATE286984T1 (de) 2005-01-15
DE60108337D1 (de) 2005-02-17
DE60108337T2 (de) 2005-12-22

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Legal Events

Date Code Title Description
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/09/2015, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 04/04/00, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO