MX379233B - Métodos y composiciones para el perfilado de adn. - Google Patents

Métodos y composiciones para el perfilado de adn.

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Publication number
MX379233B
MX379233B MX2016010717A MX2016010717A MX379233B MX 379233 B MX379233 B MX 379233B MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 379233 B MX379233 B MX 379233B
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MX
Mexico
Prior art keywords
nucleic acid
acid sample
target sequence
primers
methods
Prior art date
Application number
MX2016010717A
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English (en)
Other versions
MX2016010717A (es
Inventor
Anne Jager
Carey Davis
Cydne Holt
David Silva
Frank Steemers
Kathryn M Stephens
Min- Jui Richard Shen
Paulina Walichiewicz
Sasan Amini
Yonmee Han
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2016010717A publication Critical patent/MX2016010717A/es
Publication of MX379233B publication Critical patent/MX379233B/es

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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Las realizaciones desveladas en el presente documento proporcionan métodos para construir un perfil de ADN que comprende: una muestra de ácido nucleico, la amplificación de la muestra de ácido nucleico con una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana que comprende un polimorfismo mononucleotídico (SNP) y al menos una secuencia diana que comprende una repetición en tándem, y determinar los genotipos de dicho al menos un SNP y de dicha al menos una repetición en tándem en los productos de amplificación, construyéndose de esta manera el perfil de ADN de la muestra de ácido nucleico. Las realizaciones desveladas en el presente documento proporcionan además, una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana corta y al menos una secuencia diana larga en una muestra de ácido nucleico, en la que la amplificación de la muestra de ácido nucleico usando la pluralidad de cebadores en una sola reacción da como resultado al menos un producto de amplificación corto y al menos un producto de amplificación largo, en la que cada uno de la pluralidad de cebadores comprende una o más secuencias marcadoras.
MX2016010717A 2014-02-18 2015-02-13 Métodos y composiciones para el perfilado de adn. MX379233B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461940942P 2014-02-18 2014-02-18
US201462043060P 2014-08-28 2014-08-28
US201562103524P 2015-01-14 2015-01-14
PCT/US2015/015939 WO2015126766A1 (en) 2014-02-18 2015-02-13 Methods and compositions for dna profiling

Publications (2)

Publication Number Publication Date
MX2016010717A MX2016010717A (es) 2017-04-27
MX379233B true MX379233B (es) 2025-03-10

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US (2) US10422002B2 (es)
EP (2) EP3910069A1 (es)
JP (3) JP7011392B2 (es)
CN (1) CN106164298B (es)
AU (2) AU2015219289B2 (es)
BR (2) BR122022007092B8 (es)
CA (1) CA2940048C (es)
DK (1) DK3108009T3 (es)
ES (1) ES2866044T3 (es)
HR (1) HRP20210953T1 (es)
HU (1) HUE055256T2 (es)
LT (1) LT3108009T (es)
MX (1) MX379233B (es)
MY (2) MY182415A (es)
NZ (1) NZ723399A (es)
PL (1) PL3108009T3 (es)
PT (1) PT3108009T (es)
RS (1) RS61976B1 (es)
RU (2) RU2752700C2 (es)
SA (1) SA516371691B1 (es)
SI (1) SI3108009T1 (es)
WO (1) WO2015126766A1 (es)

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