MX368161B - Metodo para purificar el virus de rabia. - Google Patents

Metodo para purificar el virus de rabia.

Info

Publication number
MX368161B
MX368161B MX2015014700A MX2015014700A MX368161B MX 368161 B MX368161 B MX 368161B MX 2015014700 A MX2015014700 A MX 2015014700A MX 2015014700 A MX2015014700 A MX 2015014700A MX 368161 B MX368161 B MX 368161B
Authority
MX
Mexico
Prior art keywords
rabies virus
purifying
virus
substrate
exchange chromatography
Prior art date
Application number
MX2015014700A
Other languages
English (en)
Inventor
Fabre Virginie
Rocca Céline
Riffard Pierre
Calvosa Eric
Original Assignee
Sanofi Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Pasteur filed Critical Sanofi Pasteur
Publication of MX368161B publication Critical patent/MX368161B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/205—Rhabdoviridae, e.g. rabies virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para purificar el virus de rabia, que comprende una sola etapa de cromatografía de intercambio iónico, la etapa que es una cromatografía de intercambio catiónico de acuerdo con la cual: a) el sobrenadante de un cultivo de células infectadas con este virus se lleva en contacto con un sustrato de cromatografía de intercambio catiónico que comprende una matriz de polimetacrilato sobre la cual se han injertado grupos de sulfoisobutilo tal que el virus de rabia se enlaza a este sustrato y b) el virus se eluye del sustrato mismo.
MX2015014700A 2009-04-08 2010-04-08 Metodo para purificar el virus de rabia. MX368161B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0952310A FR2944292B1 (fr) 2009-04-08 2009-04-08 Procede de purification du virus rabique
US23139409P 2009-08-05 2009-08-05
PCT/FR2010/050679 WO2010116096A1 (fr) 2009-04-08 2010-04-08 Procede de purification du virus rabique

Publications (1)

Publication Number Publication Date
MX368161B true MX368161B (es) 2019-09-20

Family

ID=41219745

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015014700A MX368161B (es) 2009-04-08 2010-04-08 Metodo para purificar el virus de rabia.

Country Status (12)

Country Link
US (2) US8961997B2 (es)
EP (2) EP2806024B1 (es)
CN (2) CN102282253B (es)
AU (1) AU2010233602B2 (es)
CA (1) CA2757895A1 (es)
DK (1) DK2417251T3 (es)
ES (1) ES2498726T3 (es)
FR (1) FR2944292B1 (es)
MX (1) MX368161B (es)
PL (1) PL2417251T3 (es)
PT (1) PT2417251E (es)
WO (1) WO2010116096A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2944292B1 (fr) 2009-04-08 2013-08-23 Sanofi Pasteur Procede de purification du virus rabique
US8557253B2 (en) * 2009-10-07 2013-10-15 Sanofi Pasteur Sa Stabilizing excipient for inactivated whole virus vaccine
WO2012082723A2 (en) * 2010-12-15 2012-06-21 Emd Millipore Corporation Purification of immunogens using a non-polysaccharide matrix
US9902942B2 (en) * 2013-02-22 2018-02-27 Agency For Science, Technology And Research Chromatographic purification of virus preparations with negatively charged particles
RU2768045C2 (ru) * 2016-07-21 2022-03-23 Спарк Терапьютикс, Инк. СПОСОБ ПОЛУЧЕНИЯ ЧАСТИЦ РЕКОМБИНАНТНОГО АДЕНОАССОЦИИРОВАННОГО ВИРУСНОГО ВЕКТОРА (rAAV)
EP4382183A3 (en) * 2017-03-06 2024-08-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
EP3420076B1 (en) * 2017-03-06 2024-02-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
CN108434106B (zh) * 2017-04-25 2021-07-30 广州瑞贝斯药业有限公司 一种狂犬病疫苗的冻干制剂
CN107384877B (zh) * 2017-08-18 2020-09-08 深圳源兴基因技术有限公司 一种慢病毒的纯化方法
CN111527202B (zh) * 2017-11-08 2024-03-29 蓝天免疫疗法有限责任公司 So3色谱在病毒纯化方法中的应用
TWI894135B (zh) 2019-01-25 2025-08-21 德商百靈佳殷格翰國際股份有限公司 編碼ccl21之重組棒狀病毒
CN111088223A (zh) * 2019-12-27 2020-05-01 华农（肇庆）生物产业技术研究院有限公司 一种df-1细胞的微载体悬浮培养方法及应用
CN111467486A (zh) * 2020-04-29 2020-07-31 江苏金迪克生物技术股份有限公司 一种狂犬病疫苗的纯化方法
CN113117067A (zh) * 2021-04-16 2021-07-16 深圳市卫光生物制品股份有限公司 一种用于去除狂犬病疫苗制品中鸡胚宿主蛋白的方法
CN114015659B (zh) * 2021-10-18 2024-02-27 江苏金迪克生物技术股份有限公司 一种狂犬病疫苗的纯化方法
CN115896342A (zh) * 2022-07-05 2023-04-04 厦门大学附属中山医院 鉴别新冠疫苗污染的荧光多靶pcr检测试剂盒及制备
CN115684391A (zh) * 2022-10-12 2023-02-03 辽宁成大生物股份有限公司 一种sec-hplc法检测狂犬疫苗纯度的方法与应用

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US4525349A (en) 1981-12-29 1985-06-25 Societe Anonyme Dite: Institut Merueux Process for the large-scale production of a vaccine against poliomyelitis and the resulting vaccine
JPS6147187A (ja) 1984-08-10 1986-03-07 Chemo Sero Therapeut Res Inst 狂犬病ウイルスの精製方法
US4664912A (en) 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
FR2633832B1 (fr) 1988-07-05 1991-05-31 Virbac Vaccin antirabique avirulent
FR2693655B1 (fr) 1992-07-20 1994-10-14 Virbac Vaccin antirabique avirulent.
FR2737730B1 (fr) * 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
MY125870A (en) 1997-07-25 2006-08-30 Alza Corp Osmotic delivery system flow modulator apparatus and method
FR2801900B1 (fr) 1999-12-03 2004-02-27 Pasteur Merieux Serums Vacc Methode de production de cellules animales adherentes
ES2257505T3 (es) 2001-04-23 2006-08-01 Akzo Nobel N.V. Mutantes de virus de la rabia atenuados y vacunas vivas correspondientes.
JP2007523621A (ja) 2003-06-18 2007-08-23 オニックス ファーマシューティカルズ，インコーポレイティド ウイルスを精製するための方法
AU2005227320B2 (en) 2004-03-22 2010-06-24 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, National Insitutes Of Health Cellular and viral inactivation
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EP1724338A1 (en) * 2005-05-19 2006-11-22 Crucell Holland B.V. Methods for the production of a whole-inactivated West Nile Virus vaccine
WO2008071774A1 (en) * 2006-12-14 2008-06-19 Cytos Biotechnology Ag Purification process for coat protein of rna bacteriophages
CN101270350A (zh) 2008-03-05 2008-09-24 辽宁依生生物制药有限公司 一种提取狂犬病病毒的方法
FR2944292B1 (fr) 2009-04-08 2013-08-23 Sanofi Pasteur Procede de purification du virus rabique
CN101623497A (zh) * 2009-08-05 2010-01-13 长春卫尔赛生物药业有限公司 一种狂犬病疫苗去除残余dna的方法

Also Published As

Publication number Publication date
EP2417251A1 (fr) 2012-02-15
ES2498726T3 (es) 2014-09-25
CN105012947B (zh) 2018-11-06
EP2806024A1 (fr) 2014-11-26
PT2417251E (pt) 2014-09-09
US20100260798A1 (en) 2010-10-14
AU2010233602B2 (en) 2015-07-23
AU2010233602A1 (en) 2011-11-03
EP2417251B1 (fr) 2014-07-02
CN102282253B (zh) 2015-06-17
FR2944292A1 (fr) 2010-10-15
CN102282253A (zh) 2011-12-14
FR2944292B1 (fr) 2013-08-23
US8961997B2 (en) 2015-02-24
EP2806024B1 (fr) 2016-07-20
DK2417251T3 (da) 2014-09-15
CN105012947A (zh) 2015-11-04
CA2757895A1 (en) 2010-10-14
WO2010116096A1 (fr) 2010-10-14
US20150216963A1 (en) 2015-08-06
PL2417251T3 (pl) 2014-11-28
US9750800B2 (en) 2017-09-05

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