WO2007120624A8 - Concerted nucleic acid assembly reactions - Google Patents

Concerted nucleic acid assembly reactions

Info

Publication number
WO2007120624A8
WO2007120624A8 PCT/US2007/008785 US2007008785W WO2007120624A8 WO 2007120624 A8 WO2007120624 A8 WO 2007120624A8 US 2007008785 W US2007008785 W US 2007008785W WO 2007120624 A8 WO2007120624 A8 WO 2007120624A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
concerted
assembly reactions
acid assembly
assembly
Prior art date
Application number
PCT/US2007/008785
Other languages
French (fr)
Other versions
WO2007120624A3 (en
WO2007120624A2 (en
Inventor
Bettina Strack-Logue
William J Blake
Brian M Baynes
Original Assignee
Codon Devices Inc
Bettina Strack-Logue
William J Blake
Brian M Baynes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codon Devices Inc, Bettina Strack-Logue, William J Blake, Brian M Baynes filed Critical Codon Devices Inc
Publication of WO2007120624A2 publication Critical patent/WO2007120624A2/en
Publication of WO2007120624A8 publication Critical patent/WO2007120624A8/en
Publication of WO2007120624A3 publication Critical patent/WO2007120624A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Abstract

Certain aspects of the present invention provide methods for increasing the efficiency of nucleic acid assembly reactions by using concerted assembly to covalently connect a plurality of nucleic acid fragments in a single procedure. Aspects of the invention also provide kits, compositions, devices, and systems for designing and assembling nucleic acid products using concerted assembly procedures.
PCT/US2007/008785 2006-04-10 2007-04-10 Concerted nucleic acid assembly reactions WO2007120624A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79118306P 2006-04-10 2006-04-10
US60/791,183 2006-04-10

Publications (3)

Publication Number Publication Date
WO2007120624A2 WO2007120624A2 (en) 2007-10-25
WO2007120624A8 true WO2007120624A8 (en) 2007-12-27
WO2007120624A3 WO2007120624A3 (en) 2008-04-03

Family

ID=38610116

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/008785 WO2007120624A2 (en) 2006-04-10 2007-04-10 Concerted nucleic acid assembly reactions

Country Status (1)

Country Link
WO (1) WO2007120624A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
CA3132011A1 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP2014526899A (en) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
WO2023147547A1 (en) * 2022-01-31 2023-08-03 Integrated Dna Technologies, Inc. Recombination-based dna assembly methods and compositions

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2317865A1 (en) * 2000-03-07 2001-09-07 University Of Guelph Dna joining method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides

Also Published As

Publication number Publication date
WO2007120624A3 (en) 2008-04-03
WO2007120624A2 (en) 2007-10-25

Similar Documents

Publication Publication Date Title
WO2007120624A8 (en) Concerted nucleic acid assembly reactions
WO2009061381A3 (en) Alpha-amylase variants with altered properties
WO2008130629A3 (en) Engineered nucleases and their uses for nucleic acid assembly
WO2008027558A3 (en) Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008052101A3 (en) Multiplex automated genome engineering
WO2010127304A3 (en) Sequencing methods
WO2007123742A3 (en) Methods and compositions for increasing the fidelity of multiplex nucleic acid assembly
WO2007136874A3 (en) Genomic library construction
WO2007139723A8 (en) Chemicallymodified oligonucleotide primers for nucleic acid amplification
WO2008067423A3 (en) Methods of improving the introduction of dna into bacterial cells
WO2011072246A3 (en) Tal effector-mediated dna modification
WO2007130644A3 (en) Gimballed attachment for multiple heat exchangers
WO2005052170A3 (en) Gene silencing by using micro-rna molecules
WO2007112264A3 (en) Cleavable linkers for polynucleotides
WO2007121252A3 (en) Tandem photovoltaic cells
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
WO2009074875A8 (en) Sound-absorbing, resistant panels and process for making same
WO2010027497A3 (en) Preparations, compositions, and methods for nucleic acid sequencing
WO2010039802A3 (en) Methods and compositions for isolating nucleic acid
WO2008045460A3 (en) Maize microrna sequences
WO2007103382A3 (en) Plant-specific genetic elements and transfer cassettes for plant transformation
WO2011160052A3 (en) Methods and compositions for sequence specific rna endonucleases
WO2008043543A3 (en) Nucleic acid beacons for fluorescent in-situ hybridisation and chip technology
WO2011044458A8 (en) Compositions and methods for diagnosing genome related diseases and disorders
WO2010022194A3 (en) Compositions and methods for generation of pluripotent stem cells

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07755149

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07755149

Country of ref document: EP

Kind code of ref document: A2