MX363519B - Vectores de expresión para una secreción de proteína mejorada. - Google Patents
Vectores de expresión para una secreción de proteína mejorada.Info
- Publication number
- MX363519B MX363519B MX2016008931A MX2016008931A MX363519B MX 363519 B MX363519 B MX 363519B MX 2016008931 A MX2016008931 A MX 2016008931A MX 2016008931 A MX2016008931 A MX 2016008931A MX 363519 B MX363519 B MX 363519B
- Authority
- MX
- Mexico
- Prior art keywords
- acid sequence
- amino acid
- protein
- seq
- expression vectors
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 239000013604 expression vector Substances 0.000 title abstract 2
- 230000028327 secretion Effects 0.000 title abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 6
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a mejorar la secreción de una proteína a partir de una célula hospedera a fin de incrementar el rendimiento de producto de proteína en un proceso de fermentación. Esto se logra mediante un vector de expresión que comprende a) una secuencia de promotor y b) una secuencia de ácido nucleico que codifica para una proteína. La proteína comprende un péptido de señal y una secuencia de aminoácidos adicional, y el péptido de señal comprende una secuencia de aminoácidos que es por lo menos 80% idéntica a la secuencia de aminoácidos especificada en SEQ ID NO: 2, por lo menos 80% idéntica a la secuencia de aminoácidos especificada en SEQ ID NO: 4, por lo menos 80% idéntica a la secuencia de aminoácidos especificada en SEQ ID NO: 6, o el péptido de señal comprende una secuencia de aminoácidos que es estructuralmente homóloga a por lo menos una de dichas secuencias.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102011118032A DE102011118032A1 (de) | 2011-05-31 | 2011-05-31 | Expressionsvektoren zur verbesserten Proteinsekretion |
| PCT/EP2012/059901 WO2012163855A1 (de) | 2011-05-31 | 2012-05-25 | Expressionsvektoren zur verbesserten proteinsekretion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX363519B true MX363519B (es) | 2019-03-26 |
Family
ID=46149497
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013013616A MX340485B (es) | 2011-05-31 | 2012-05-25 | Vectores de expresion para una secrecion de proteina mejorada. |
| MX2016008931A MX363519B (es) | 2011-05-31 | 2012-05-25 | Vectores de expresión para una secreción de proteína mejorada. |
| MX2019003360A MX2019003360A (es) | 2011-05-31 | 2013-11-21 | Vectores de expresion para una secrecion de proteina mejorada. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013013616A MX340485B (es) | 2011-05-31 | 2012-05-25 | Vectores de expresion para una secrecion de proteina mejorada. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019003360A MX2019003360A (es) | 2011-05-31 | 2013-11-21 | Vectores de expresion para una secrecion de proteina mejorada. |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US9803183B2 (es) |
| EP (3) | EP2714902B1 (es) |
| JP (2) | JP6324309B2 (es) |
| KR (1) | KR101956142B1 (es) |
| CN (2) | CN103649310A (es) |
| BR (1) | BR112013030846A2 (es) |
| CA (1) | CA2835746A1 (es) |
| DE (1) | DE102011118032A1 (es) |
| DK (3) | DK3118310T3 (es) |
| ES (2) | ES2606553T3 (es) |
| MX (3) | MX340485B (es) |
| PL (1) | PL2714902T3 (es) |
| RU (1) | RU2661790C2 (es) |
| WO (1) | WO2012163855A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102011118032A1 (de) * | 2011-05-31 | 2012-12-06 | Henkel Ag & Co. Kgaa | Expressionsvektoren zur verbesserten Proteinsekretion |
| WO2017089093A1 (en) | 2015-11-25 | 2017-06-01 | Unilever N.V. | A liquid detergent composition |
| CN108367245A (zh) | 2015-12-09 | 2018-08-03 | 巴斯夫欧洲公司 | 在解吸条件下从发酵固体纯化蛋白质的方法 |
| CN109477065A (zh) | 2016-07-14 | 2019-03-15 | 巴斯夫欧洲公司 | 包含螯合剂的发酵培养基 |
| EP3707255A1 (en) | 2017-11-09 | 2020-09-16 | Basf Se | Coatings of enzyme particles comprising organic white pigments |
| CN111373039A (zh) | 2017-11-29 | 2020-07-03 | 丹尼斯科美国公司 | 具有改善的稳定性的枯草杆菌蛋白酶变体 |
| EP3887515A1 (en) | 2018-11-28 | 2021-10-06 | Danisco US Inc. | Subtilisin variants having improved stability |
| RU2747627C1 (ru) * | 2020-05-18 | 2021-05-11 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный университет" | Рекомбинантная плазмида pUSB2-AmQ, обеспечивающая синтез белка альфа-амилазы Bacillus amyloliquefaciens, и штамм Bacillus subtilis/pUSB2-AmQ - продуцент белка альфа-амилазы Bacillus amyloliquefaciens |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0493398T3 (da) | 1989-08-25 | 2000-05-22 | Henkel Research Corp | Alkalisk, proteolytisk enzym og fremgangsmåde til fremstilling deraf |
| US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| EP0551408B2 (en) | 1991-06-11 | 2012-08-15 | Genencor International, Inc. | Detergent compositions containing cellulase compositions deficient in cbh i type components |
| DK82893D0 (da) * | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
| ATE315083T1 (de) | 1995-03-17 | 2006-02-15 | Novozymes As | Neue endoglukanase |
| ES2525677T3 (es) | 1995-10-17 | 2014-12-29 | Ab Enzymes Oy | Celulasas, genes que las codifican y usos de las mismas |
| CN101085985B (zh) | 1996-09-17 | 2012-05-16 | 诺沃奇梅兹有限公司 | 纤维素酶变体 |
| DE19713852A1 (de) | 1997-04-04 | 1998-10-08 | Henkel Kgaa | Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln |
| CN1234854C (zh) | 1999-03-31 | 2006-01-04 | 诺维信公司 | 具有碱性α－淀粉酶活性的多肽以及编码该多肽的核酸 |
| EP1240525A2 (en) | 1999-12-23 | 2002-09-18 | PHARMACIA & UPJOHN COMPANY | Sodium channels as targets for amyloid beta |
| DE60143086D1 (de) | 2000-08-04 | 2010-10-28 | Genencor Int | Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung |
| US20030049619A1 (en) * | 2001-03-21 | 2003-03-13 | Simon Delagrave | Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides |
| ES2521615T3 (es) | 2001-06-06 | 2014-11-13 | Novozymes A/S | Endo-beta-1,4-glucanasa |
| DE10131441A1 (de) | 2001-06-29 | 2003-01-30 | Henkel Kgaa | Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen |
| DE10162727A1 (de) * | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10163748A1 (de) | 2001-12-21 | 2003-07-17 | Henkel Kgaa | Neue Glykosylhydrolasen |
| DE10260903A1 (de) | 2002-12-20 | 2004-07-08 | Henkel Kgaa | Neue Perhydrolasen |
| US20060236414A1 (en) * | 2003-06-19 | 2006-10-19 | Novozymes A/S | Proteases and methods for producing them |
| CN103333870A (zh) | 2003-12-03 | 2013-10-02 | 丹尼斯科美国公司 | 过水解酶 |
| CN1926431A (zh) * | 2004-01-09 | 2007-03-07 | 诺维信股份有限公司 | 地衣芽孢杆菌染色体 |
| EP2287179A3 (en) * | 2004-01-09 | 2011-05-18 | Novozymes A/S | Increased bacillus YweA expression |
| DE102004029475A1 (de) | 2004-06-18 | 2006-01-26 | Henkel Kgaa | Neues enzymatisches Bleichsystem |
| DE102006038448A1 (de) | 2005-12-28 | 2008-02-21 | Henkel Kgaa | Enzym-haltiges Reinigungsmittel |
| CN101460618A (zh) | 2006-04-20 | 2009-06-17 | 诺维信公司 | 对蛋渍具有改进的洗涤性能的洒维奈斯变体 |
| DE102006022224A1 (de) | 2006-05-11 | 2007-11-15 | Henkel Kgaa | Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin |
| EP2099818A2 (en) * | 2006-11-29 | 2009-09-16 | Novozymes Inc. | Bacillus licheniformis chromosome |
| DE102007003143A1 (de) | 2007-01-16 | 2008-07-17 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE102007049830A1 (de) | 2007-10-16 | 2009-04-23 | Henkel Ag & Co. Kgaa | Neue Proteinvarianten durch zirkulare Permutation |
| DK2462224T3 (en) * | 2009-08-03 | 2017-09-04 | C-Lecta Gmbh | PROCEDURE FOR MANUFACTURING NUCLEASES OF A GRAM NEGATIVE BACTERY WHEN USING A GRAM POSITIVE EXPRESSION HOST |
| DE102009029513A1 (de) * | 2009-09-16 | 2011-03-24 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen |
| DE102011007313A1 (de) | 2011-04-13 | 2012-10-18 | Henkel Ag & Co. Kgaa | Expressionsverfahren |
| DE102011118032A1 (de) * | 2011-05-31 | 2012-12-06 | Henkel Ag & Co. Kgaa | Expressionsvektoren zur verbesserten Proteinsekretion |
| DE102012201297A1 (de) | 2012-01-31 | 2013-08-01 | Basf Se | Expressionsverfahren |
-
2011
- 2011-05-31 DE DE102011118032A patent/DE102011118032A1/de not_active Withdrawn
-
2012
- 2012-05-25 BR BR112013030846A patent/BR112013030846A2/pt not_active Application Discontinuation
- 2012-05-25 EP EP12723512.5A patent/EP2714902B1/de not_active Not-in-force
- 2012-05-25 KR KR1020137034520A patent/KR101956142B1/ko active IP Right Grant
- 2012-05-25 CN CN201280026365.0A patent/CN103649310A/zh active Pending
- 2012-05-25 US US14/122,562 patent/US9803183B2/en active Active
- 2012-05-25 EP EP18212311.7A patent/EP3527661B1/de active Active
- 2012-05-25 EP EP16178440.0A patent/EP3118310B1/de active Active
- 2012-05-25 DK DK16178440.0T patent/DK3118310T3/da active
- 2012-05-25 CA CA2835746A patent/CA2835746A1/en not_active Abandoned
- 2012-05-25 ES ES12723512.5T patent/ES2606553T3/es active Active
- 2012-05-25 MX MX2013013616A patent/MX340485B/es active IP Right Grant
- 2012-05-25 RU RU2013158458A patent/RU2661790C2/ru not_active IP Right Cessation
- 2012-05-25 WO PCT/EP2012/059901 patent/WO2012163855A1/de active Application Filing
- 2012-05-25 PL PL12723512T patent/PL2714902T3/pl unknown
- 2012-05-25 DK DK12723512.5T patent/DK2714902T3/en active
- 2012-05-25 DK DK18212311.7T patent/DK3527661T3/da active
- 2012-05-25 JP JP2014513143A patent/JP6324309B2/ja active Active
- 2012-05-25 MX MX2016008931A patent/MX363519B/es unknown
- 2012-05-25 CN CN201710933102.2A patent/CN107574177B/zh active Active
- 2012-05-25 ES ES16178440T patent/ES2763577T3/es active Active
-
2013
- 2013-11-21 MX MX2019003360A patent/MX2019003360A/es active IP Right Grant
-
2017
- 2017-03-17 JP JP2017052565A patent/JP6522030B2/ja not_active Expired - Fee Related
- 2017-09-22 US US15/712,652 patent/US10494622B2/en active Active
-
2019
- 2019-12-02 US US16/700,322 patent/US11046961B2/en active Active
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