PL2714902T3 - Wektory ekspresyjne do polepszonej sekrecji białka - Google Patents

Wektory ekspresyjne do polepszonej sekrecji białka

Info

Publication number
PL2714902T3
PL2714902T3 PL12723512T PL12723512T PL2714902T3 PL 2714902 T3 PL2714902 T3 PL 2714902T3 PL 12723512 T PL12723512 T PL 12723512T PL 12723512 T PL12723512 T PL 12723512T PL 2714902 T3 PL2714902 T3 PL 2714902T3
Authority
PL
Poland
Prior art keywords
expression vectors
protein secretion
improved protein
improved
secretion
Prior art date
Application number
PL12723512T
Other languages
English (en)
Inventor
Christian Degering
Thorsten Eggert
Stefan Evers
Karl-Heinz Maurer
Johannes Bongaerts
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of PL2714902T3 publication Critical patent/PL2714902T3/pl

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • C12N15/625DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
PL12723512T 2011-05-31 2012-05-25 Wektory ekspresyjne do polepszonej sekrecji białka PL2714902T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102011118032A DE102011118032A1 (de) 2011-05-31 2011-05-31 Expressionsvektoren zur verbesserten Proteinsekretion

Publications (1)

Publication Number Publication Date
PL2714902T3 true PL2714902T3 (pl) 2017-02-28

Family

ID=46149497

Family Applications (1)

Application Number Title Priority Date Filing Date
PL12723512T PL2714902T3 (pl) 2011-05-31 2012-05-25 Wektory ekspresyjne do polepszonej sekrecji białka

Country Status (14)

Country Link
US (3) US9803183B2 (pl)
EP (3) EP3118310B1 (pl)
JP (2) JP6324309B2 (pl)
KR (1) KR101956142B1 (pl)
CN (2) CN103649310A (pl)
BR (1) BR112013030846A2 (pl)
CA (1) CA2835746A1 (pl)
DE (1) DE102011118032A1 (pl)
DK (3) DK2714902T3 (pl)
ES (2) ES2606553T3 (pl)
MX (3) MX340485B (pl)
PL (1) PL2714902T3 (pl)
RU (1) RU2661790C2 (pl)
WO (1) WO2012163855A1 (pl)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
US10731111B2 (en) 2015-11-25 2020-08-04 Conopco, Inc. Liquid laundry detergent composition
JP2018536418A (ja) 2015-12-09 2018-12-13 ビーエーエスエフ ソシエタス・ヨーロピアBasf Se 脱離条件下で発酵固体からタンパク質を精製する方法
MX2019000621A (es) 2016-07-14 2019-08-01 Basf Se Medio de fermentacion que comprende agente quelante.
CN111315879A (zh) 2017-11-09 2020-06-19 巴斯夫欧洲公司 包含有机白色颜料的酶颗粒涂层
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
RU2747627C1 (ru) * 2020-05-18 2021-05-11 федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный университет" Рекомбинантная плазмида pUSB2-AmQ, обеспечивающая синтез белка альфа-амилазы Bacillus amyloliquefaciens, и штамм Bacillus subtilis/pUSB2-AmQ - продуцент белка альфа-амилазы Bacillus amyloliquefaciens

Family Cites Families (33)

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ES2144990T3 (es) 1989-08-25 2000-07-01 Henkel Of America Inc Enzima proteolitica alcalina y metodo de produccion.
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
DK0877077T3 (da) 1991-06-11 2010-09-06 Genencor Int Vaskemiddel-sammensætning indeholdende cellulase-sammensætninger, som er fattige for CBH I-type-bestanddele
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
EP1683860B1 (en) 1995-03-17 2013-10-23 Novozymes A/S Novel endoglucanases
ES2525677T3 (es) 1995-10-17 2014-12-29 Ab Enzymes Oy Celulasas, genes que las codifican y usos de las mismas
JP3532576B2 (ja) 1996-09-17 2004-05-31 ノボザイムス アクティーゼルスカブ セルラーゼ変異体
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
EP2011864B1 (en) 1999-03-31 2014-12-31 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
PE20010978A1 (es) 1999-12-23 2001-09-14 Upjohn Co ENSAYOS Y METODOS DE DIAGNOSTICO QUE INVOLUCRAN CANALES DE SODIO COMO OBJETIVOS DE AMILOIDE ß O DE SUS AGREGADOS
DK1305432T3 (da) 2000-08-04 2010-12-06 Genencor Int Muterede trichoderma reesei EGIII-cellulaser, DNA, der koder for sådanne EGIII-sammensætninger og fremgangsmåder til at frembringe samme
US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
MXPA03011194A (es) 2001-06-06 2004-02-26 Novozymes As Endo-beta-1,4-glucanasa.
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
DE10162727A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
US20060236414A1 (en) * 2003-06-19 2006-10-19 Novozymes A/S Proteases and methods for producing them
ES2361838T3 (es) 2003-12-03 2011-06-22 Danisco Us Inc. Perhidrolasa.
EP2287179A3 (en) * 2004-01-09 2011-05-18 Novozymes A/S Increased bacillus YweA expression
CN1926431A (zh) * 2004-01-09 2007-03-07 诺维信股份有限公司 地衣芽孢杆菌染色体
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
EP2013339B1 (en) * 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
WO2008066931A2 (en) * 2006-11-29 2008-06-05 Novozymes, Inc. Bacillus licheniformis chromosome
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE102007049830A1 (de) * 2007-10-16 2009-04-23 Henkel Ag & Co. Kgaa Neue Proteinvarianten durch zirkulare Permutation
DK2462224T3 (en) * 2009-08-03 2017-09-04 C-Lecta Gmbh PROCEDURE FOR MANUFACTURING NUCLEASES OF A GRAM NEGATIVE BACTERY WHEN USING A GRAM POSITIVE EXPRESSION HOST
DE102009029513A1 (de) * 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen
DE102011007313A1 (de) 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
DE102012201297A1 (de) 2012-01-31 2013-08-01 Basf Se Expressionsverfahren

Also Published As

Publication number Publication date
JP6522030B2 (ja) 2019-05-29
BR112013030846A2 (pt) 2016-11-29
EP3118310B1 (de) 2019-09-25
JP6324309B2 (ja) 2018-05-16
US11046961B2 (en) 2021-06-29
RU2013158458A (ru) 2015-07-10
KR101956142B1 (ko) 2019-03-11
MX340485B (es) 2016-07-08
ES2763577T3 (es) 2020-05-29
US20140356929A1 (en) 2014-12-04
DE102011118032A1 (de) 2012-12-06
JP2017127317A (ja) 2017-07-27
MX363519B (es) 2019-03-26
US20180073005A1 (en) 2018-03-15
EP2714902A1 (de) 2014-04-09
US10494622B2 (en) 2019-12-03
KR20140034866A (ko) 2014-03-20
RU2661790C2 (ru) 2018-07-19
DK3118310T3 (da) 2019-12-16
CN107574177A (zh) 2018-01-12
DK2714902T3 (en) 2017-01-09
DK3527661T3 (da) 2022-03-07
WO2012163855A1 (de) 2012-12-06
CA2835746A1 (en) 2012-12-06
US20200095564A1 (en) 2020-03-26
EP3527661B1 (de) 2021-12-01
MX2013013616A (es) 2014-01-08
JP2014519327A (ja) 2014-08-14
MX2019003360A (es) 2019-07-04
EP3118310A1 (de) 2017-01-18
ES2606553T3 (es) 2017-03-24
US9803183B2 (en) 2017-10-31
EP3527661A1 (de) 2019-08-21
CN103649310A (zh) 2014-03-19
CN107574177B (zh) 2022-01-11
EP2714902B1 (de) 2016-09-14

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