MX347050B - Un método para preparar una levadura industrial, levadura industrial y aplicación a la producción de etanol a partir de, por lo menos, una pentosa. - Google Patents

Un método para preparar una levadura industrial, levadura industrial y aplicación a la producción de etanol a partir de, por lo menos, una pentosa.

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Publication number
MX347050B
MX347050B MX2013006209A MX2013006209A MX347050B MX 347050 B MX347050 B MX 347050B MX 2013006209 A MX2013006209 A MX 2013006209A MX 2013006209 A MX2013006209 A MX 2013006209A MX 347050 B MX347050 B MX 347050B
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MX
Mexico
Prior art keywords
ethanol
industrial yeast
yeast
pentose
strains
Prior art date
Application number
MX2013006209A
Other languages
English (en)
Other versions
MX2013006209A (es
Inventor
Thomas Desfougeres
Georges Pignede
Original Assignee
Lesaffre & Cie
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Publication date
Application filed by Lesaffre & Cie filed Critical Lesaffre & Cie
Publication of MX2013006209A publication Critical patent/MX2013006209A/es
Publication of MX347050B publication Critical patent/MX347050B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01009—D-Xylulose reductase (1.1.1.9), i.e. xylitol dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01021—Aldehyde reductase (1.1.1.21), i.e. aldose-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una cepa de lavadura, caracterizada porque comprende genes exógenos de la vía metabólica de xilosa, en donde el gen exógeno de la vía metabólica de xilosa presente en la cepa de levadura consiste de al menos un copia de un gen exógeno que codifica una xilosa isomerasa, y una copia de un gen exógeno que codifica una xilitol deshidrogenasa, y porque ha experimentado un paso posterior de evolución dirigida que comprende los siguientes pasos sucesivos que consisten en someter dicha cepa de levadura a: (i) una mutagénesis, (ii) un crecimiento en cultivos cíclicos, bajo O2 limitado en un medio que comprende al menos una pentosa, especialmente xilosa, y (iii) una selección por crecimiento aeróbico en medio sólido que contiene glicerol como única fuente de carbono.
MX2013006209A 2010-12-03 2011-12-02 Un método para preparar una levadura industrial, levadura industrial y aplicación a la producción de etanol a partir de, por lo menos, una pentosa. MX347050B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1004709A FR2968313B1 (fr) 2010-12-03 2010-12-03 Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose
PCT/EP2011/071616 WO2012072793A1 (fr) 2010-12-03 2011-12-02 Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose

Publications (2)

Publication Number Publication Date
MX2013006209A MX2013006209A (es) 2013-07-15
MX347050B true MX347050B (es) 2017-04-07

Family

ID=44310212

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013006209A MX347050B (es) 2010-12-03 2011-12-02 Un método para preparar una levadura industrial, levadura industrial y aplicación a la producción de etanol a partir de, por lo menos, una pentosa.

Country Status (17)

Country Link
US (1) US9309524B2 (es)
EP (1) EP2646544B1 (es)
AR (1) AR084087A1 (es)
AU (1) AU2011334846B2 (es)
BR (1) BR112013013696B1 (es)
CA (1) CA2819323C (es)
CL (1) CL2013001569A1 (es)
DK (1) DK2646544T3 (es)
ES (1) ES2608784T3 (es)
FR (1) FR2968313B1 (es)
HR (1) HRP20161752T1 (es)
HU (1) HUE031826T2 (es)
MX (1) MX347050B (es)
MY (1) MY180908A (es)
PL (1) PL2646544T3 (es)
PT (1) PT2646544T (es)
WO (1) WO2012072793A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2991339B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Souches de levure aptes a metaboliser le xylose et resistantes a au moins un inhibiteur de fermentation, procede d'obtention et utilisation
DK2877576T3 (da) 2012-07-24 2019-08-12 Bp Corp North America Inc Xyloseisomeraser og anvendelser deraf
FR2996855B1 (fr) 2012-10-16 2016-11-11 Lesaffre & Cie Souches de levures pour la production de biomasse sur un substrat comprenant un sucre en c5, leurs procedes d'obtention et utilisations de la biomasse produite
FR3017623B1 (fr) 2014-02-17 2018-08-10 Lesaffre Et Compagnie Souche fermentant les pentoses a propagation optimisee
FR3035405B1 (fr) 2015-04-27 2019-04-19 Lesaffre Et Compagnie Souche de levure presentant une capacite amelioree a fermenter le xylose en presence d'acide acetique
FR3036709B1 (fr) 2015-05-29 2019-06-07 Lesaffre Et Compagnie Propagation de levures simultanee a la saccharification
BR112018000690A2 (pt) 2015-07-13 2018-09-18 MARA Renewables Corporation micro-organismo recombinante, e, métodos para preparar um micro-organismo de metabolização de xilose e para produzir óleo.
FR3055635A1 (fr) 2016-09-08 2018-03-09 Lesaffre Et Compagnie Utilisation de qtl pour conferer a des souches de levure une resistance a l'acide acetique
FR3055634B1 (fr) 2016-09-08 2021-03-05 Lesaffre & Cie Utilisation de mcm7 pour obtenir des souches de levure resistantes a l'acide acetique
FR3062134B1 (fr) 2017-01-24 2023-07-21 Lesaffre & Cie Obtention de souches de levure performantes pour la metabolisation de l'arabinose
JP7350659B2 (ja) 2017-06-06 2023-09-26 ザイマージェン インコーポレイテッド Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａ ｓｐｉｎｏｓａの改良のためのハイスループット（ＨＴＰ）ゲノム操作プラットフォーム
BE1024788B1 (nl) * 2017-08-02 2018-06-27 Anheuser-Busch Inbev S.A. Werkwijze voor het beheren en verzenden van gist voor de productie van gefermenteerde dranken
CN109504695B (zh) * 2019-01-02 2022-08-09 中国农业科学院作物科学研究所 多中心单鞭毛菌木糖异构酶基因及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866382A (en) * 1990-04-06 1999-02-02 Xyrofin Oy Xylose utilization by recombinant yeasts
FR2675815B1 (fr) 1991-04-23 1994-11-04 Lesaffre & Cie Nouvelles souches de levure de panification et leur procede d'obtention, nouvelles levures fraiche et seche correspondantes.
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
WO2004046333A2 (en) 2002-11-15 2004-06-03 Novozymes North America, Inc. Ethanol production by simultaneous saccharification and fermentation (ssf)
WO2005113774A2 (en) 2004-05-19 2005-12-01 Biotechnology Research And Development Corporation Methods for production of xylitol in microorganisms
MX2009003501A (es) 2006-10-02 2009-04-16 Dsm Ip Assets Bv Ingenieria metabolica de celulas eucariotas que fermentan arabinosa.
WO2008133665A2 (en) 2006-12-07 2008-11-06 Massachusetts Institute Of Technology Global transcription machinery engineering
US20090246844A1 (en) * 2007-10-26 2009-10-01 Arbor Fuel Inc. Methods for the production of ethanol
BRPI0910812A2 (pt) 2008-03-07 2019-02-26 Dsm Ip Assets Bv célula de fermentação de açúcar pentose
DE102008031350B4 (de) 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen

Also Published As

Publication number Publication date
FR2968313B1 (fr) 2014-10-10
AU2011334846B2 (en) 2016-12-15
FR2968313A1 (fr) 2012-06-08
CL2013001569A1 (es) 2014-03-28
MX2013006209A (es) 2013-07-15
EP2646544A1 (fr) 2013-10-09
EP2646544B1 (fr) 2016-11-02
PL2646544T3 (pl) 2017-04-28
HUE031826T2 (hu) 2017-08-28
MY180908A (en) 2020-12-11
WO2012072793A1 (fr) 2012-06-07
US20120142067A1 (en) 2012-06-07
CA2819323C (fr) 2019-08-20
PT2646544T (pt) 2016-12-30
BR112013013696B1 (pt) 2021-04-13
AU2011334846A1 (en) 2013-06-13
BR112013013696A2 (pt) 2017-06-06
ES2608784T3 (es) 2017-04-17
CA2819323A1 (fr) 2012-06-07
DK2646544T3 (da) 2017-01-16
AR084087A1 (es) 2013-04-17
US9309524B2 (en) 2016-04-12
HRP20161752T1 (hr) 2017-02-24

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