MX346663B - Lineas de celulas de bajo nivel de fucosa y usos de las mismas. - Google Patents

Lineas de celulas de bajo nivel de fucosa y usos de las mismas.

Info

Publication number
MX346663B
MX346663B MX2013010210A MX2013010210A MX346663B MX 346663 B MX346663 B MX 346663B MX 2013010210 A MX2013010210 A MX 2013010210A MX 2013010210 A MX2013010210 A MX 2013010210A MX 346663 B MX346663 B MX 346663B
Authority
MX
Mexico
Prior art keywords
cells
fucose
population
recombinant proteins
binding agent
Prior art date
Application number
MX2013010210A
Other languages
English (en)
Other versions
MX2013010210A (es
Inventor
Daniel Helman
Meirav Bar-Shimon
Mira Toister-Achituv
Original Assignee
Merck Serono Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL211583A external-priority patent/IL211583A0/en
Priority claimed from IL217216A external-priority patent/IL217216A0/en
Application filed by Merck Serono Sa filed Critical Merck Serono Sa
Publication of MX2013010210A publication Critical patent/MX2013010210A/es
Publication of MX346663B publication Critical patent/MX346663B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01068—Glycoprotein 6-alpha-L-fucosyltransferase (2.4.1.68), i.e. FUT8
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01047—GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método de selección de células que tienen cero nivel de fucosa, útiles como células hospederas para la expresión de proteínas recombinantes; el método comprende: (d) introducir mutaciones genéticas en una población de células CHO poniendo en contacto las células con metotrexato (MTX), (e) poner en contacto la población de células CHO que comprenden células mutadas con un agente que se une a la fucosa no tóxico por una cantidad de tiempo que permita la unión del agente que se une a la fucosa a una porción de fucosa sobre la membrana de una célula de la población de células, en donde la cantidad de tiempo no permita la destrucción de las células; y (f) agotar de la población de células que comprenden células mutadas, una subpoblación de células que se unen al agente que se une a la fucosa, seleccionando de esta manera células útiles como células hospederas para la expresión de proteínas recombinantes, las células seleccionadas teniendo cero contenido de fucosa; se describen también células y líneas de células útiles como células hospederas para la expresión de proteínas recombinantes.
MX2013010210A 2011-03-06 2012-03-05 Lineas de celulas de bajo nivel de fucosa y usos de las mismas. MX346663B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL211583A IL211583A0 (en) 2011-03-06 2011-03-06 Low fucose cell lines and uses thereof
IL217216A IL217216A0 (en) 2011-12-27 2011-12-27 Low fucose cell lins and uses thereof
PCT/IL2012/000109 WO2012120500A2 (en) 2011-03-06 2012-03-05 Low fucose cell lines and uses thereof

Publications (2)

Publication Number Publication Date
MX2013010210A MX2013010210A (es) 2013-09-26
MX346663B true MX346663B (es) 2017-03-27

Family

ID=45952581

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013010210A MX346663B (es) 2011-03-06 2012-03-05 Lineas de celulas de bajo nivel de fucosa y usos de las mismas.

Country Status (14)

Country Link
US (1) US9574003B2 (es)
EP (1) EP2683821B1 (es)
JP (2) JP2014509850A (es)
CN (2) CN109797138A (es)
AU (1) AU2012226398B9 (es)
BR (1) BR112013022832A2 (es)
CA (1) CA2829110C (es)
EA (1) EA032513B1 (es)
ES (1) ES2687771T3 (es)
IL (1) IL228184B (es)
MX (1) MX346663B (es)
SG (1) SG192632A1 (es)
WO (1) WO2012120500A2 (es)
ZA (1) ZA201306336B (es)

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Also Published As

Publication number Publication date
EP2683821B1 (en) 2018-07-25
EA032513B1 (ru) 2019-06-28
IL228184B (en) 2018-12-31
AU2012226398B9 (en) 2017-04-13
AU2012226398A1 (en) 2013-09-12
SG192632A1 (en) 2013-09-30
CA2829110A1 (en) 2012-09-13
CN103597073B (zh) 2019-06-07
CA2829110C (en) 2019-01-15
US9574003B2 (en) 2017-02-21
AU2012226398B2 (en) 2017-03-23
JP2017079775A (ja) 2017-05-18
BR112013022832A2 (pt) 2016-11-22
ES2687771T3 (es) 2018-10-29
JP2014509850A (ja) 2014-04-24
ZA201306336B (en) 2014-10-29
JP6353023B2 (ja) 2018-07-04
CN109797138A (zh) 2019-05-24
EA201391282A1 (ru) 2014-04-30
MX2013010210A (es) 2013-09-26
WO2012120500A2 (en) 2012-09-13
US20140005368A1 (en) 2014-01-02
CN103597073A (zh) 2014-02-19
WO2012120500A3 (en) 2012-11-15
EP2683821A2 (en) 2014-01-15

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