MX344407B - Analisis rapdo y preciso de la sialilacion de proteinas. - Google Patents
Analisis rapdo y preciso de la sialilacion de proteinas.Info
- Publication number
- MX344407B MX344407B MX2014000378A MX2014000378A MX344407B MX 344407 B MX344407 B MX 344407B MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 344407 B MX344407 B MX 344407B
- Authority
- MX
- Mexico
- Prior art keywords
- gluco
- sialylation
- protein
- galactose
- rapid
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
La presente invención se refiere a un método para determinar el porcentaje de glactosa recubierta en una glicoproteína, que comprende: (a) preparar una glicoproteína en un medio para análisis, con lo cual se obtiene una preparación proteica; (b) preparar muestras de proteína enzimáticamente tratada, y un control de la proteína preparada que comprende: dividir la preparación de proteína en una pluralidad de muestras de proteína; (i) no agregar enzima a por lo menos una muestra de proteína como un control antecedente de muestra de medio, con lo cual se obtiene la muestra A; (ii) agregar al menos ß-galactosidadas a por lo menos una muestra de proteína, con lo cual se obtiene la muestra B; (iii) agregar al menos ß-galactosidada y asialidasa a por lo menos otra muestra de proteína, con lo cual se obtiene la muestra C; e incubar la pluralidad de muestras de proteína que comprenden las muestras A, B y C; (c) cuantificar la cantidad de galactosa en cada una de las muestras A, B y C usando cromatografía HPAEC-PAD, en donde la cantidad de galactosa en la muestra A refleja la cantidad de galactosa en el medio, en donde la cantidad de galactosa en la muestra B refleja la cantidad de galactosa no recubierta con un residuo de ácido siálico en la preparación de proteína además de la cantidad de galactosa del medio, y en donde la cantidad de galactosa en la muestra C refleja la cantidad de galactosa recubierta con un ácido siálico además de la cantidad de galactosa del medio y no recubierta por ácido siálico; y (d) determinar el porcentaje de galactosa recubierta en la glicoproteína con base en las cantidades de galactosa en la muestra A, muestra B y muestra C, en donde el método tiene la linealidad de R2= 0.99.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161507643P | 2011-07-14 | 2011-07-14 | |
| PCT/ES2012/070501 WO2013011178A1 (es) | 2011-07-14 | 2012-07-05 | Análisis rápido y preciso de la sialilación de proteínas |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014000378A MX2014000378A (es) | 2014-03-31 |
| MX344407B true MX344407B (es) | 2016-12-14 |
Family
ID=47557705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014000378A MX344407B (es) | 2011-07-14 | 2012-07-05 | Analisis rapdo y preciso de la sialilacion de proteinas. |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US9260743B2 (es) |
| EP (1) | EP2733218B1 (es) |
| JP (1) | JP2014525742A (es) |
| KR (1) | KR101864468B1 (es) |
| CN (1) | CN103635590A (es) |
| AR (1) | AR087175A1 (es) |
| AU (1) | AU2012285696B2 (es) |
| BR (1) | BR112013033883B1 (es) |
| CA (1) | CA2837981C (es) |
| CL (1) | CL2013003713A1 (es) |
| ES (1) | ES2675511T3 (es) |
| HU (1) | HUE038020T2 (es) |
| IL (1) | IL229828A (es) |
| MX (1) | MX344407B (es) |
| MY (1) | MY163078A (es) |
| PL (1) | PL2733218T3 (es) |
| PT (1) | PT2733218T (es) |
| RU (1) | RU2605900C2 (es) |
| SG (1) | SG10201500002XA (es) |
| TR (1) | TR201809082T4 (es) |
| UY (1) | UY34195A (es) |
| WO (1) | WO2013011178A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2675511T3 (es) * | 2011-07-14 | 2018-07-11 | Grifols, S.A. | Análisis rápido y preciso de la sialilación de proteínas |
| CN106324234B (zh) * | 2016-08-08 | 2018-05-22 | 上海睿康生物科技有限公司 | 修饰的n-乙酰神经氨酸醛缩酶及其制备方法和应用 |
| CN107045019B (zh) * | 2016-09-30 | 2019-08-16 | 中国医学科学院输血研究所 | 一种IVIG中IgG Fab片段和Fc片段唾液酸含量的测定方法 |
| CN110346500B (zh) * | 2018-04-04 | 2021-11-30 | 青岛大学附属医院 | 一种基于微波酸水解的阴离子交换色谱-脉冲安培法检测血清中单糖含量的检测方法 |
| CN109329713B (zh) * | 2018-12-01 | 2022-04-22 | 西华大学 | 一种降解牦牛肉中n-羟乙酰神经唾液酸的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5570677B2 (ja) * | 2002-04-25 | 2014-08-13 | シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド | α−ガラクトシダーゼＡ欠損症の治療 |
| ES2214166T1 (es) * | 2002-09-11 | 2004-09-16 | Fresenius Kabi Deutschland Gmbh | Polipeptidos has-ilados, especialmente, eriptropoyetina has-ilada. |
| JP2008539413A (ja) * | 2005-04-26 | 2008-11-13 | レイモンド， エー． ドウェック， | 自動化糖鎖フィンガープリント戦略 |
| US8039208B2 (en) * | 2005-04-26 | 2011-10-18 | National Institute For Bioprocessing Research And Training Limited (Nibrt) | Automated strategy for identifying physiological glycosylation markers(s) |
| ES2517603T3 (es) * | 2008-04-07 | 2014-11-03 | Bayer Healthcare, Llc | Procedimientos de producción recombinante de glucoproteínas |
| US20110086362A1 (en) * | 2009-10-09 | 2011-04-14 | Massachusetts Institute Of Technology | High-Throughput Method for Quantifying Sialylation of Glycoproteins |
| ES2675511T3 (es) * | 2011-07-14 | 2018-07-11 | Grifols, S.A. | Análisis rápido y preciso de la sialilación de proteínas |
-
2012
- 2012-07-05 ES ES12815322.8T patent/ES2675511T3/es active Active
- 2012-07-05 MX MX2014000378A patent/MX344407B/es active IP Right Grant
- 2012-07-05 KR KR1020147003822A patent/KR101864468B1/ko active IP Right Grant
- 2012-07-05 PT PT128153228T patent/PT2733218T/pt unknown
- 2012-07-05 MY MYPI2013702595A patent/MY163078A/en unknown
- 2012-07-05 RU RU2013154278/10A patent/RU2605900C2/ru active
- 2012-07-05 BR BR112013033883-0A patent/BR112013033883B1/pt not_active IP Right Cessation
- 2012-07-05 TR TR2018/09082T patent/TR201809082T4/tr unknown
- 2012-07-05 CN CN201280033128.7A patent/CN103635590A/zh active Pending
- 2012-07-05 CA CA2837981A patent/CA2837981C/en active Active
- 2012-07-05 WO PCT/ES2012/070501 patent/WO2013011178A1/es active Application Filing
- 2012-07-05 US US14/232,783 patent/US9260743B2/en active Active
- 2012-07-05 SG SG10201500002XA patent/SG10201500002XA/en unknown
- 2012-07-05 PL PL12815322T patent/PL2733218T3/pl unknown
- 2012-07-05 JP JP2014519588A patent/JP2014525742A/ja active Pending
- 2012-07-05 EP EP12815322.8A patent/EP2733218B1/en active Active
- 2012-07-05 HU HUE12815322A patent/HUE038020T2/hu unknown
- 2012-07-05 AU AU2012285696A patent/AU2012285696B2/en active Active
- 2012-07-12 UY UY0001034195A patent/UY34195A/es active IP Right Grant
- 2012-07-13 AR ARP120102538A patent/AR087175A1/es active IP Right Grant
-
2013
- 2013-12-05 IL IL229828A patent/IL229828A/en active IP Right Grant
- 2013-12-24 CL CL2013003713A patent/CL2013003713A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2014525742A (ja) | 2014-10-02 |
| AU2012285696B2 (en) | 2015-12-10 |
| RU2013154278A (ru) | 2015-06-20 |
| WO2013011178A1 (es) | 2013-01-24 |
| CN103635590A (zh) | 2014-03-12 |
| AR087175A1 (es) | 2014-02-26 |
| UY34195A (es) | 2013-02-28 |
| MX2014000378A (es) | 2014-03-31 |
| PL2733218T3 (pl) | 2018-08-31 |
| BR112013033883A2 (pt) | 2017-02-14 |
| BR112013033883B1 (pt) | 2020-11-03 |
| CL2013003713A1 (es) | 2014-08-08 |
| KR20140060289A (ko) | 2014-05-19 |
| CA2837981A1 (en) | 2013-01-24 |
| RU2605900C2 (ru) | 2016-12-27 |
| MY163078A (en) | 2017-08-15 |
| AU2012285696A1 (en) | 2013-05-09 |
| EP2733218A4 (en) | 2015-03-25 |
| NZ618889A (en) | 2015-08-28 |
| TR201809082T4 (tr) | 2018-07-23 |
| US9260743B2 (en) | 2016-02-16 |
| CA2837981C (en) | 2020-08-18 |
| EP2733218B1 (en) | 2018-05-30 |
| HUE038020T2 (hu) | 2018-09-28 |
| US20140162299A1 (en) | 2014-06-12 |
| KR101864468B1 (ko) | 2018-06-04 |
| IL229828A (en) | 2016-09-29 |
| PT2733218T (pt) | 2018-07-04 |
| EP2733218A1 (en) | 2014-05-21 |
| ES2675511T3 (es) | 2018-07-11 |
| SG10201500002XA (en) | 2015-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |