MX2014000378A - Analisis rapdo y preciso de la sialilacion de proteinas. - Google Patents

Analisis rapdo y preciso de la sialilacion de proteinas.

Info

Publication number
MX2014000378A
MX2014000378A MX2014000378A MX2014000378A MX2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A
Authority
MX
Mexico
Prior art keywords
gluco
sialylation
protein
galactose
rapid
Prior art date
Application number
MX2014000378A
Other languages
English (en)
Other versions
MX344407B (es
Inventor
Zihao Wang
Jessica Sloan
Kevin Wee
Original Assignee
Grifols Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grifols Sa filed Critical Grifols Sa
Publication of MX2014000378A publication Critical patent/MX2014000378A/es
Publication of MX344407B publication Critical patent/MX344407B/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Métodos y kits para el análisis de la sialilación de glucoproteínas. Las muestras de glucoproteína se incuban por separado en tres condiciones: con beta-galactosidasa, con beta-galactosidasa + alfa-sialidasa, y sin enzima. Tras el tratamiento enzimático, se determinan cuantitativamente mediante cromatografía de intercambio aniónico de alta resolución con detección amperométrica pulsada (HPAEC PAD) la galactosa total en la muestra, la galactosa no sialilada y la galactosa exógena del medio. La determinación de estos valores permite deducir el porcentaje de sialilación de la glucoproteína.
MX2014000378A 2011-07-14 2012-07-05 Analisis rapdo y preciso de la sialilacion de proteinas. MX344407B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161507643P 2011-07-14 2011-07-14
PCT/ES2012/070501 WO2013011178A1 (es) 2011-07-14 2012-07-05 Análisis rápido y preciso de la sialilación de proteínas

Publications (2)

Publication Number Publication Date
MX2014000378A true MX2014000378A (es) 2014-03-31
MX344407B MX344407B (es) 2016-12-14

Family

ID=47557705

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000378A MX344407B (es) 2011-07-14 2012-07-05 Analisis rapdo y preciso de la sialilacion de proteinas.

Country Status (22)

Country Link
US (1) US9260743B2 (es)
EP (1) EP2733218B1 (es)
JP (1) JP2014525742A (es)
KR (1) KR101864468B1 (es)
CN (1) CN103635590A (es)
AR (1) AR087175A1 (es)
AU (1) AU2012285696B2 (es)
BR (1) BR112013033883B1 (es)
CA (1) CA2837981C (es)
CL (1) CL2013003713A1 (es)
ES (1) ES2675511T3 (es)
HU (1) HUE038020T2 (es)
IL (1) IL229828A (es)
MX (1) MX344407B (es)
MY (1) MY163078A (es)
PL (1) PL2733218T3 (es)
PT (1) PT2733218T (es)
RU (1) RU2605900C2 (es)
SG (1) SG10201500002XA (es)
TR (1) TR201809082T4 (es)
UY (1) UY34195A (es)
WO (1) WO2013011178A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2675511T3 (es) * 2011-07-14 2018-07-11 Grifols, S.A. Análisis rápido y preciso de la sialilación de proteínas
CN106324234B (zh) * 2016-08-08 2018-05-22 上海睿康生物科技有限公司 修饰的n-乙酰神经氨酸醛缩酶及其制备方法和应用
CN107045019B (zh) * 2016-09-30 2019-08-16 中国医学科学院输血研究所 一种IVIG中IgG Fab片段和Fc片段唾液酸含量的测定方法
CN110346500B (zh) * 2018-04-04 2021-11-30 青岛大学附属医院 一种基于微波酸水解的阴离子交换色谱-脉冲安培法检测血清中单糖含量的检测方法
CN109329713B (zh) * 2018-12-01 2022-04-22 西华大学 一种降解牦牛肉中n-羟乙酰神经唾液酸的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5570677B2 (ja) * 2002-04-25 2014-08-13 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド α−ガラクトシダーゼＡ欠損症の治療
ES2214166T1 (es) * 2002-09-11 2004-09-16 Fresenius Kabi Deutschland Gmbh Polipeptidos has-ilados, especialmente, eriptropoyetina has-ilada.
JP2008539413A (ja) * 2005-04-26 2008-11-13 レイモンド， エー． ドウェック， 自動化糖鎖フィンガープリント戦略
US8039208B2 (en) * 2005-04-26 2011-10-18 National Institute For Bioprocessing Research And Training Limited (Nibrt) Automated strategy for identifying physiological glycosylation markers(s)
ES2517603T3 (es) * 2008-04-07 2014-11-03 Bayer Healthcare, Llc Procedimientos de producción recombinante de glucoproteínas
US20110086362A1 (en) * 2009-10-09 2011-04-14 Massachusetts Institute Of Technology High-Throughput Method for Quantifying Sialylation of Glycoproteins
ES2675511T3 (es) * 2011-07-14 2018-07-11 Grifols, S.A. Análisis rápido y preciso de la sialilación de proteínas

Also Published As

Publication number Publication date
JP2014525742A (ja) 2014-10-02
AU2012285696B2 (en) 2015-12-10
RU2013154278A (ru) 2015-06-20
WO2013011178A1 (es) 2013-01-24
CN103635590A (zh) 2014-03-12
AR087175A1 (es) 2014-02-26
UY34195A (es) 2013-02-28
PL2733218T3 (pl) 2018-08-31
BR112013033883A2 (pt) 2017-02-14
BR112013033883B1 (pt) 2020-11-03
CL2013003713A1 (es) 2014-08-08
KR20140060289A (ko) 2014-05-19
CA2837981A1 (en) 2013-01-24
RU2605900C2 (ru) 2016-12-27
MY163078A (en) 2017-08-15
MX344407B (es) 2016-12-14
AU2012285696A1 (en) 2013-05-09
EP2733218A4 (en) 2015-03-25
NZ618889A (en) 2015-08-28
TR201809082T4 (tr) 2018-07-23
US9260743B2 (en) 2016-02-16
CA2837981C (en) 2020-08-18
EP2733218B1 (en) 2018-05-30
HUE038020T2 (hu) 2018-09-28
US20140162299A1 (en) 2014-06-12
KR101864468B1 (ko) 2018-06-04
IL229828A (en) 2016-09-29
PT2733218T (pt) 2018-07-04
EP2733218A1 (en) 2014-05-21
ES2675511T3 (es) 2018-07-11
SG10201500002XA (en) 2015-02-27

Similar Documents

Publication Publication Date Title
EP4249605A3 (en) Methods for analyte detection
WO2012155019A8 (en) Multiple reaction monitoring lc-ms/ms method to detect therapeutic antibodies in animal samples using framework signature peptides
WO2010083252A3 (en) Ratio based biomarkers and methods of use thereof
MX363201B (es) Procedimiento, matriz y uso para determinar la presencia de cancer pancreatico.
WO2015128272A3 (en) Photo-selective method for biological sample analysis field
MY163078A (en) Rapid and accurate analysis of protein sialylation
BR112015014833B8 (pt) Anticorpo isolado ou fragmento de ligação, ácido nucleico, método in vitro, kit e composição de anticorpo
PH12014501138A1 (en) Il-1 binding proteins
MY198081A (en) Urinalysis device and dry reagent for quantitative urinalysis
WO2012097081A3 (en) Protein detection method
NZ621733A (en) Lung cancer biomarkers and uses thereof
EP3955004A3 (en) Use of fatty acids in methods for detecting cancer
CA2855971C (en) Methods for detecting human papillomavirus and providing prognosis for head and neck squamous cell carcinoma
IN2014KN02647A (es)
WO2012056236A3 (en) Analytical methods and arrays for use in the identification of agents inducing sensitization in human skin
EP3851856A3 (en) Method, array and use thereof
NZ609824A (en) Immunochromatography devices, methods and kits
GB201207297D0 (en) Analytical methods and arrays for use in the same
TR201819546T4 (tr) Numune tür kökeni için tespit yöntemleri.
WO2009055823A3 (en) Method to predict responsiveness of breast cancer to polyaminetype chemotherapy
MX357429B (es) Predictores para el tratamiento del cáncer.
SG10201807867XA (en) Simultaneous detection of target protein and target nucleic acids in a single cell
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
MX2009001070A (es) Uso de proteina con actividad inhibidora de melanoma (mia) como un indicador temprano para la respuesta terapeutica en melanoma.
EP3998274A4 (en) METHOD OF PREPARATION OF A SAMPLE TO BE ANALYZED, METHOD OF ANALYSIS AND KIT FOR THE PREPARATION OF A SAMPLE TO BE ANALYZED

Legal Events

Date Code Title Description
FG Grant or registration