MX2014000378A - Analisis rapdo y preciso de la sialilacion de proteinas. - Google Patents

Analisis rapdo y preciso de la sialilacion de proteinas.

Info

Publication number
MX2014000378A
MX2014000378A MX2014000378A MX2014000378A MX2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A
Authority
MX
Mexico
Prior art keywords
gluco
sialylation
protein
galactose
rapid
Prior art date
Application number
MX2014000378A
Other languages
English (en)
Other versions
MX344407B (es
Inventor
Zihao Wang
Jessica Sloan
Kevin Wee
Original Assignee
Grifols Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grifols Sa filed Critical Grifols Sa
Publication of MX2014000378A publication Critical patent/MX2014000378A/es
Publication of MX344407B publication Critical patent/MX344407B/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Métodos y kits para el análisis de la sialilación de glucoproteínas. Las muestras de glucoproteína se incuban por separado en tres condiciones: con beta-galactosidasa, con beta-galactosidasa + alfa-sialidasa, y sin enzima. Tras el tratamiento enzimático, se determinan cuantitativamente mediante cromatografía de intercambio aniónico de alta resolución con detección amperométrica pulsada (HPAEC PAD) la galactosa total en la muestra, la galactosa no sialilada y la galactosa exógena del medio. La determinación de estos valores permite deducir el porcentaje de sialilación de la glucoproteína.
MX2014000378A 2011-07-14 2012-07-05 Analisis rapdo y preciso de la sialilacion de proteinas. MX344407B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161507643P 2011-07-14 2011-07-14
PCT/ES2012/070501 WO2013011178A1 (es) 2011-07-14 2012-07-05 Análisis rápido y preciso de la sialilación de proteínas

Publications (2)

Publication Number Publication Date
MX2014000378A true MX2014000378A (es) 2014-03-31
MX344407B MX344407B (es) 2016-12-14

Family

ID=47557705

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000378A MX344407B (es) 2011-07-14 2012-07-05 Analisis rapdo y preciso de la sialilacion de proteinas.

Country Status (22)

Country Link
US (1) US9260743B2 (es)
EP (1) EP2733218B1 (es)
JP (1) JP2014525742A (es)
KR (1) KR101864468B1 (es)
CN (1) CN103635590A (es)
AR (1) AR087175A1 (es)
AU (1) AU2012285696B2 (es)
BR (1) BR112013033883B1 (es)
CA (1) CA2837981C (es)
CL (1) CL2013003713A1 (es)
ES (1) ES2675511T3 (es)
HU (1) HUE038020T2 (es)
IL (1) IL229828A (es)
MX (1) MX344407B (es)
MY (1) MY163078A (es)
PL (1) PL2733218T3 (es)
PT (1) PT2733218T (es)
RU (1) RU2605900C2 (es)
SG (1) SG10201500002XA (es)
TR (1) TR201809082T4 (es)
UY (1) UY34195A (es)
WO (1) WO2013011178A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2605900C2 (ru) * 2011-07-14 2016-12-27 Грайфолз С.А. Быстрый и точный анализ сиалирования белков
CN106324234B (zh) * 2016-08-08 2018-05-22 上海睿康生物科技有限公司 修饰的n-乙酰神经氨酸醛缩酶及其制备方法和应用
CN107045019B (zh) * 2016-09-30 2019-08-16 中国医学科学院输血研究所 一种IVIG中IgG Fab片段和Fc片段唾液酸含量的测定方法
CN110346500B (zh) * 2018-04-04 2021-11-30 青岛大学附属医院 一种基于微波酸水解的阴离子交换色谱-脉冲安培法检测血清中单糖含量的检测方法
CN109329713B (zh) * 2018-12-01 2022-04-22 西华大学 一种降解牦牛肉中n-羟乙酰神经唾液酸的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2367257T3 (es) * 2002-04-25 2011-10-31 Shire Human Genetic Therapies, Inc. Tratamiento de deficit de alfa-galactosidasa a.
ES2214166T1 (es) * 2002-09-11 2004-09-16 Fresenius Kabi Deutschland Gmbh Polipeptidos has-ilados, especialmente, eriptropoyetina has-ilada.
US8039208B2 (en) * 2005-04-26 2011-10-18 National Institute For Bioprocessing Research And Training Limited (Nibrt) Automated strategy for identifying physiological glycosylation markers(s)
JP2008539413A (ja) * 2005-04-26 2008-11-13 レイモンド， エー． ドウェック， 自動化糖鎖フィンガープリント戦略
WO2009126564A1 (en) * 2008-04-07 2009-10-15 Bayer Healthcare Llc Methods of recombinant production of glycoproteins
US20110086362A1 (en) 2009-10-09 2011-04-14 Massachusetts Institute Of Technology High-Throughput Method for Quantifying Sialylation of Glycoproteins
RU2605900C2 (ru) * 2011-07-14 2016-12-27 Грайфолз С.А. Быстрый и точный анализ сиалирования белков

Also Published As

Publication number Publication date
PL2733218T3 (pl) 2018-08-31
HUE038020T2 (hu) 2018-09-28
SG10201500002XA (en) 2015-02-27
UY34195A (es) 2013-02-28
RU2013154278A (ru) 2015-06-20
MX344407B (es) 2016-12-14
PT2733218T (pt) 2018-07-04
AU2012285696A1 (en) 2013-05-09
EP2733218A4 (en) 2015-03-25
IL229828A (en) 2016-09-29
US9260743B2 (en) 2016-02-16
WO2013011178A1 (es) 2013-01-24
JP2014525742A (ja) 2014-10-02
ES2675511T3 (es) 2018-07-11
AR087175A1 (es) 2014-02-26
CL2013003713A1 (es) 2014-08-08
KR20140060289A (ko) 2014-05-19
EP2733218A1 (en) 2014-05-21
CN103635590A (zh) 2014-03-12
US20140162299A1 (en) 2014-06-12
CA2837981C (en) 2020-08-18
MY163078A (en) 2017-08-15
AU2012285696B2 (en) 2015-12-10
BR112013033883A2 (pt) 2017-02-14
BR112013033883B1 (pt) 2020-11-03
CA2837981A1 (en) 2013-01-24
NZ618889A (en) 2015-08-28
KR101864468B1 (ko) 2018-06-04
RU2605900C2 (ru) 2016-12-27
EP2733218B1 (en) 2018-05-30
TR201809082T4 (tr) 2018-07-23

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