MX343462B - Medio del cultivo celular. - Google Patents

Medio del cultivo celular.

Info

Publication number
MX343462B
MX343462B MX2013006958A MX2013006958A MX343462B MX 343462 B MX343462 B MX 343462B MX 2013006958 A MX2013006958 A MX 2013006958A MX 2013006958 A MX2013006958 A MX 2013006958A MX 343462 B MX343462 B MX 343462B
Authority
MX
Mexico
Prior art keywords
laminin
stem cells
single cell
embryonic stem
recombinant
Prior art date
Application number
MX2013006958A
Other languages
English (en)
Other versions
MX2013006958A (es
Inventor
Tryggvason Karl
Rodin Sergey
Original Assignee
Biolamina Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biolamina Ab filed Critical Biolamina Ab
Publication of MX2013006958A publication Critical patent/MX2013006958A/es
Publication of MX343462B publication Critical patent/MX343462B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/13—Hollow or container type article [e.g., tube, vase, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Transplantation (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a un sistema para mantener células madre pluripotentes, caracterizado porque comprende: un medio de cultivo celular que comprende de 0.5 a 3.5 ng/ml del factor de crecimiento del fibroblasto básico (bFGF); y un sustrato que contiene laminina-521 para proporcionar soporte a las células madre.
MX2013006958A 2010-12-17 2011-12-16 Medio del cultivo celular. MX343462B (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201061424273P 2010-12-17 2010-12-17
US201161488353P 2011-05-20 2011-05-20
US201161533063P 2011-09-09 2011-09-09
US201161537940P 2011-09-22 2011-09-22
US201161565380P 2011-11-30 2011-11-30
US201161565849P 2011-12-01 2011-12-01
PCT/IB2011/003265 WO2012080844A1 (en) 2010-12-17 2011-12-16 Cell culture medium

Publications (2)

Publication Number Publication Date
MX2013006958A MX2013006958A (es) 2013-12-16
MX343462B true MX343462B (es) 2016-11-07

Family

ID=45755415

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2013006958A MX343462B (es) 2010-12-17 2011-12-16 Medio del cultivo celular.
MX2013006959A MX345861B (es) 2010-12-17 2011-12-16 Laminina-521 recombinante.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2013006959A MX345861B (es) 2010-12-17 2011-12-16 Laminina-521 recombinante.

Country Status (12)

Country Link
US (2) US8415156B2 (es)
EP (2) EP2651973B1 (es)
JP (2) JP6002675B2 (es)
KR (3) KR101921350B1 (es)
CN (2) CN103384679B (es)
AU (1) AU2011342904B2 (es)
BR (2) BR112013017629A2 (es)
CA (2) CA2826252C (es)
IL (2) IL226971B (es)
MX (2) MX343462B (es)
SG (3) SG191216A1 (es)
WO (2) WO2012080844A1 (es)

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ES2530995T3 (es) 2007-09-28 2015-03-09 Anthrogenesis Corp Supresión de tumor usando perfusato placentario humano y células asesinas naturales intermediarias que provienen de placenta humana
WO2011063005A2 (en) 2009-11-17 2011-05-26 Advanced Cell Technology, Inc. Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells
NZ605505A (en) 2010-07-13 2015-02-27 Anthrogenesis Corp Methods of generating natural killer cells
KR101921350B1 (ko) * 2010-12-17 2018-11-22 바이오라미나 에이비 인간 다능성 줄기세포를 유지하기 위한 시스템
US10240124B2 (en) * 2011-09-22 2019-03-26 Biolamina Ab Cell culture substrate comprising a laminin and a cadherin
US9828630B2 (en) 2011-11-30 2017-11-28 Biolamina Ab Methods of enhancing genetic diagnosis
EP3282009A1 (en) 2012-04-20 2018-02-14 Biolamina AB Maintenance of differentiated cells with laminins
US20130330825A1 (en) * 2012-06-07 2013-12-12 City Of Hope Attachment substrates for directed differentiation of human embryonic stem cells in culture
EP2892996B1 (en) * 2012-09-07 2019-08-14 BioLamina AB Stem cell bank
WO2014087244A2 (en) * 2012-12-03 2014-06-12 Biolamina Ab Methods of producing rpe cells
JP2016506968A (ja) 2013-02-05 2016-03-07 アントフロゲネシス コーポレーション 胎盤由来のナチュラルキラー細胞
EP3546568A3 (en) * 2013-07-02 2019-10-23 National University of Singapore Differentiation of pluripotent stem cells and cardiac progenitor cells into striated cardiomyocyte fibers using laminins ln-511, ln-521 and ln-221
WO2015064795A1 (ko) * 2013-11-01 2015-05-07 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포
US11624053B2 (en) 2013-11-27 2023-04-11 Kyoto Prefectural Public University Corporation Application of laminin to corneal endothelial cell culture
US11633477B2 (en) 2014-10-31 2023-04-25 Kyoto Prefectural Public University Corporation Treatment of cornea using laminin
JP6770435B2 (ja) * 2014-10-31 2020-10-14 京都府公立大学法人 ラミニンによる網膜および神経の新規治療
HUE041518T2 (hu) * 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához
US20190359939A1 (en) * 2015-08-23 2019-11-28 Diamyd Medical Ab New use
US10683486B2 (en) * 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
JP6605618B2 (ja) * 2015-11-10 2019-11-20 日機装株式会社 細胞支持複合体および細胞支持複合体の製造方法
AU2017240591B2 (en) 2016-03-30 2023-08-24 Asterias Biotherapeutics, Inc. Oligodendrocyte progenitor cell compositions
US20190211305A1 (en) * 2016-08-25 2019-07-11 Kyoto University Method for Culturing Pluripotent Stem Cells on Specific Laminin
RU2758006C2 (ru) * 2016-11-16 2021-10-25 Сината Терапьютикс Лимитед Анализ плюрипотентных стволовых клеток
CN106591295B (zh) * 2016-12-31 2019-10-25 贵州北科生物科技有限公司 一种多功能干细胞培养方法及所用培养基
EP3619293B1 (en) 2017-05-05 2023-11-29 National University of Singapore Methods for culturing human keratinocytes
EP3914264A4 (en) 2019-01-23 2022-11-23 Asterias Biotherapeutics, Inc. DORSAL DERIVATIVE OLIGODENDROCYTE PROGENITORS FROM HUMAN PLURIPOTENTIC STEM CELLS
CN111617105A (zh) * 2019-04-03 2020-09-04 成熙(上海)生物科技有限公司 一种脂肪干细胞多细胞活性因子冻干粉的制备方法
CN111621473A (zh) * 2019-04-03 2020-09-04 成熙(上海)生物科技有限公司 一种新型人源脂肪干细胞制剂的制备方法
WO2021233116A1 (zh) * 2020-05-21 2021-11-25 北京大学 多能干细胞的成分明确、无异源的培养系统
CN111569048B (zh) * 2020-05-22 2023-04-18 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 LamG5肽在制备修复睾丸中支持细胞损伤的药物中的应用

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US8951799B2 (en) * 2007-01-04 2015-02-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent stem cells
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JP5765714B2 (ja) 2009-05-29 2015-08-19 国立大学法人京都大学 人工多能性幹細胞の製造方法および培養方法
WO2010140698A1 (ja) * 2009-06-03 2010-12-09 国立大学法人東北大学 多能性幹細胞からの神経堤細胞群の分化誘導方法
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KR101921350B1 (ko) 2010-12-17 2018-11-22 바이오라미나 에이비 인간 다능성 줄기세포를 유지하기 위한 시스템

Also Published As

Publication number Publication date
CA2826252C (en) 2018-12-04
JP2013545489A (ja) 2013-12-26
SG10201510405PA (en) 2016-01-28
KR20140016881A (ko) 2014-02-10
AU2011342904B2 (en) 2016-05-12
EP2651974B1 (en) 2015-09-16
KR20190018024A (ko) 2019-02-20
CA2828794C (en) 2021-07-13
IL226971B (en) 2018-04-30
EP2651973B1 (en) 2016-08-31
MX345861B (es) 2017-02-20
IL270186B (en) 2020-08-31
CA2828794A1 (en) 2012-06-21
JP5990531B2 (ja) 2016-09-14
EP2651973A1 (en) 2013-10-23
SG191215A1 (en) 2013-07-31
KR20140012047A (ko) 2014-01-29
JP2014501101A (ja) 2014-01-20
US8415156B2 (en) 2013-04-09
SG191216A1 (en) 2013-07-31
US20120156782A1 (en) 2012-06-21
JP6002675B2 (ja) 2016-10-05
WO2012080842A1 (en) 2012-06-21
CN103384680A (zh) 2013-11-06
BR112013017632A2 (pt) 2016-10-11
BR112013017629A2 (pt) 2017-04-18
MX2013006958A (es) 2013-12-16
US20120156254A1 (en) 2012-06-21
CN103384679B (zh) 2015-08-26
WO2012080844A1 (en) 2012-06-21
EP2651974A1 (en) 2013-10-23
CN103384680B (zh) 2015-08-26
KR101921350B1 (ko) 2018-11-22
CA2826252A1 (en) 2012-06-21
US10138292B2 (en) 2018-11-27
CN103384679A (zh) 2013-11-06
MX2013006959A (es) 2013-12-06
KR101966033B1 (ko) 2019-04-04

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