MX2023001762A - Genes de resistencia a multiples enfermedades y apilamientos genomicos de los mismos. - Google Patents

Genes de resistencia a multiples enfermedades y apilamientos genomicos de los mismos.

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Publication number
MX2023001762A
MX2023001762A MX2023001762A MX2023001762A MX2023001762A MX 2023001762 A MX2023001762 A MX 2023001762A MX 2023001762 A MX2023001762 A MX 2023001762A MX 2023001762 A MX2023001762 A MX 2023001762A MX 2023001762 A MX2023001762 A MX 2023001762A
Authority
MX
Mexico
Prior art keywords
disease resistance
resistance genes
stacks
genomic
multiple disease
Prior art date
Application number
MX2023001762A
Other languages
English (en)
Inventor
Michael Lassner
Huirong Gao
Bailin Li
Mary J Frank
Petra J Wolters
Jeffrey Habben
Girma M Tabor
Sabrina Humbert
Nandini Krishnamurthy
Robert B Meeley
Leandro Daniel Perugini
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2023001762A publication Critical patent/MX2023001762A/es

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

El campo es el de la biología molecular, y más específicamente, el de métodos para ingeniería cromosómica de múltiples genes nativos, tal como genes de resistencia a enfermedades en un locus genómico utilizando edición específica del sitio para producir plantas. También se describen en el presente documento métodos para generar un locus genómico heterólogo en una planta que comprende una pluralidad de secuencias polinucleotídicas intraespecies.
MX2023001762A 2020-08-18 2021-08-17 Genes de resistencia a multiples enfermedades y apilamientos genomicos de los mismos. MX2023001762A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063067090P 2020-08-18 2020-08-18
US202163154960P 2021-03-01 2021-03-01
PCT/US2021/046227 WO2022040134A1 (en) 2020-08-18 2021-08-17 Multiple disease resistance genes and genomic stacks thereof

Publications (1)

Publication Number Publication Date
MX2023001762A true MX2023001762A (es) 2023-02-22

Family

ID=77726530

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023001762A MX2023001762A (es) 2020-08-18 2021-08-17 Genes de resistencia a multiples enfermedades y apilamientos genomicos de los mismos.

Country Status (7)

Country Link
US (2) US20220056470A1 (es)
EP (1) EP4200425A1 (es)
CN (3) CN116134143A (es)
BR (1) BR112023002885A2 (es)
CA (3) CA3186862A1 (es)
MX (1) MX2023001762A (es)
WO (3) WO2022040134A1 (es)

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6162967A (en) 1994-01-26 2000-12-19 Pioneer Hi-Bred International, Inc. Positional cloning of soybean cyst nematode resistance genes
WO2006017841A2 (en) 2004-08-06 2006-02-16 Pioneer Hi-Bred International Inc. Genetic loci associated with fusarium solani tolerance in soybean
US8367899B2 (en) * 2007-12-31 2013-02-05 E I Du Pont De Neumours And Company Gray leaf spot tolerant maize and methods of production
CA3060736C (en) 2010-11-05 2022-11-01 Agrigenetics, Inc. Soybean marker barc 010889 01691 linked to soybean cyst nematode resistatance
CA2831144A1 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
WO2013025773A2 (en) 2011-08-16 2013-02-21 Pioneer Hi-Bred International, Inc. Molecular markers associated with soybean root-knot nematode tolerance and methods of their use
EP4289948A3 (en) 2012-05-25 2024-04-17 The Regents of the University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US20130337442A1 (en) 2012-06-15 2013-12-19 Pioneer Hi-Bred International, Inc. Genetic loci associated with soybean cyst nematode resistance and methods of use
CN103805595A (zh) * 2012-11-13 2014-05-21 南京大学 高通量克隆植物抗病基因的方法
US20140189896A1 (en) 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
US9493843B2 (en) 2012-12-20 2016-11-15 Pioneer Hi-Bred International, Inc. Genetic loci associated with Phytophthora tolerance in soybean and methods of use
US20140178866A1 (en) 2012-12-21 2014-06-26 Pioneer Hi-Bred International, Inc. Genetic loci associated with soybean cyst nematode resistance and methods of use
US9464330B2 (en) 2012-12-21 2016-10-11 Pioneer Hi-Bred International, Inc. Genetic loci associated with soybean cyst nematode resistance and methods of use
CA2845444C (en) 2013-03-12 2023-08-29 Dow Agrosciences Llc Soybean markers linked to phytophthora resistance
BR112015023134B1 (pt) 2013-03-15 2023-10-17 Pioneer Hi-Bred International, Inc Método para identificar uma planta de soja ou um germoplasma de soja que exibe resistência aprimorada à mancha foliar olho-de-rã
CA2922089C (en) 2013-08-22 2022-07-05 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
WO2016007347A1 (en) 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EP3191595B1 (en) 2014-09-12 2019-12-25 E. I. du Pont de Nemours and Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
CA2966731C (en) 2014-11-06 2023-01-31 E. I. Du Pont De Nemours And Company Peptide-mediated delivery of rna-guided endonuclease into cells
CA2987333A1 (en) 2015-06-03 2016-12-08 Dow Agrosciences Llc Genetic locus associated with phytophthora root and stem rot in soybean
US20160355840A1 (en) * 2015-06-03 2016-12-08 E I Du Pont De Nemours And Company Methods of identifying and selecting maize plants with resistance to anthracnose stalk rot
US9894857B2 (en) 2015-07-31 2018-02-20 Pioneer Hi-Bred International, Inc. Loci associated with charcoal rot drought complex tolerance in soybean
WO2017079026A1 (en) 2015-11-06 2017-05-11 E. I. Du Pont De Nemours And Company Generation of complex trait loci in soybean and methods of use
US11319599B2 (en) 2015-12-18 2022-05-03 Pioneer Hi-Bred International, Inc. Genetic loci associated with reproductive growth phenotypes in soybean and methods of use
CA3004914A1 (en) 2016-02-05 2017-08-10 Pioneer Hi-Bred International, Inc. Genetic loci associated with brown stem rot resistance in soybean and methods of use
EP3525578A1 (en) * 2016-10-13 2019-08-21 Pioneer Hi-Bred International, Inc. Generating northern leaf blight resistant maize
BR112020001844A2 (pt) 2017-09-14 2020-10-06 Pioneer Hi-Bred International, Inc. método para reduzir altura de planta, método para identificar um alelo de br1 ou um alelo dw5 em uma população de plantas, planta semianã, planta de sorgo semianã, construção de dna recombinante, célula vegetal, sequência de rna-guia planta, semente, planta de sorgo semianã modificada, planta de sorgo anã, planta de maís mutante, método para reduzir altura de planta, planta de sorgo, população de plantas de sorgo, método para reduzir a frequência de reversão de um alelo anão 3 (dw3) em uma planta de sorgo em um fenótipo alto, planta de arroz, método de seleção assistida por marcador de uma planta
CA3078845A1 (en) * 2017-10-09 2019-04-18 Pioneer Hi-Bred International, Inc. Type i-e crispr-cas systems for eukaryotic genome editing
BR112020024837A2 (pt) 2018-06-06 2021-03-02 Pioneer Hi-Bred International, Inc. métodos de identificação, seleção e produção de safras resistentes à ferrugem polissora
US20220073890A1 (en) 2018-12-14 2022-03-10 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
CN111197056B (zh) * 2020-01-09 2022-07-22 中国科学院华南植物园 棉花基因叠加目标系的建立及其应用

Also Published As

Publication number Publication date
EP4200425A1 (en) 2023-06-28
WO2023023499A1 (en) 2023-02-23
CA3228155A1 (en) 2023-02-23
BR112023002885A2 (pt) 2023-03-21
CN117998982A (zh) 2024-05-07
US20220056470A1 (en) 2022-02-24
CN117812999A (zh) 2024-04-02
CA3186862A1 (en) 2022-02-24
US20230295650A1 (en) 2023-09-21
CN116134143A (zh) 2023-05-16
WO2022040134A1 (en) 2022-02-24
WO2023023419A1 (en) 2023-02-23
CA3228149A1 (en) 2023-02-23

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