MX2022008045A - Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). - Google Patents

Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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Publication number
MX2022008045A
MX2022008045A MX2022008045A MX2022008045A MX2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A
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MX
Mexico
Prior art keywords
umis
sequences
unique molecular
dna fragments
sequenced dna
Prior art date
Application number
MX2022008045A
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English (en)
Inventor
Emrah Kostem
Sante Gnerre
Alex Aravanis
Byoungsok Jung
Zhihong Zhang
Xuyu Cai
Alex So
Frank J Steemers
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55910388&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2022008045(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2022008045A publication Critical patent/MX2022008045A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Biomedical Technology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Las realizaciones desveladas se refieren a métodos, aparatos, sistemas y productos de programa informático para determinar secuencias de interés usando secuencias de índice molecular único (IMU) que son asociables de forma única a fragmentos polinucleotídicos individuales, que incluyen secuencias con bajas frecuencias alélicas y longitud de secuencia larga. En algunas implementaciones, los IMU incluyen tanto IMU físicos (exógenos), por ejemplo introducidos usando adaptadores en forma de Y, como IMU virtuales (endógenos) presentes en los fragmentos de ADN para secuenciar. En algunas implementaciones, las secuencias de índice molecular único incluyen secuencias no aleatorias. También se proporcionan sistemas, aparatos y productos de programa informático para determinar una secuencia de interés implementando los métodos desvelados.
MX2022008045A 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). MX2022008045A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562153699P 2015-04-28 2015-04-28
US201562193469P 2015-07-16 2015-07-16
US201562269485P 2015-12-18 2015-12-18

Publications (1)

Publication Number Publication Date
MX2022008045A true MX2022008045A (es) 2022-07-27

Family

ID=55910388

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).
MX2022008045A MX2022008045A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).

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MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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US (3) US10844428B2 (es)
EP (3) EP3289097B2 (es)
JP (1) JP6685324B2 (es)
KR (1) KR102091312B1 (es)
CN (2) CN113832139A (es)
AU (3) AU2016256351B2 (es)
CA (2) CA2983935C (es)
DK (2) DK3289097T4 (es)
ES (2) ES2961338T3 (es)
FI (2) FI3289097T4 (es)
HK (1) HK1244513A1 (es)
IL (3) IL294600B2 (es)
MX (3) MX2017013775A (es)
MY (1) MY181983A (es)
NZ (1) NZ736609A (es)
RU (1) RU2704286C2 (es)
SG (2) SG10202006185QA (es)
WO (1) WO2016176091A1 (es)
ZA (1) ZA201707231B (es)

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