MX2022008045A - Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). - Google Patents

Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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Publication number
MX2022008045A
MX2022008045A MX2022008045A MX2022008045A MX2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A
Authority
MX
Mexico
Prior art keywords
umis
sequences
unique molecular
dna fragments
sequenced dna
Prior art date
Application number
MX2022008045A
Other languages
English (en)
Inventor
Emrah Kostem
Sante Gnerre
Alex Aravanis
Byoungsok Jung
Zhihong Zhang
Xuyu Cai
Alex So
Frank J Steemers
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55910388&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2022008045(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2022008045A publication Critical patent/MX2022008045A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Las realizaciones desveladas se refieren a métodos, aparatos, sistemas y productos de programa informático para determinar secuencias de interés usando secuencias de índice molecular único (IMU) que son asociables de forma única a fragmentos polinucleotídicos individuales, que incluyen secuencias con bajas frecuencias alélicas y longitud de secuencia larga. En algunas implementaciones, los IMU incluyen tanto IMU físicos (exógenos), por ejemplo introducidos usando adaptadores en forma de Y, como IMU virtuales (endógenos) presentes en los fragmentos de ADN para secuenciar. En algunas implementaciones, las secuencias de índice molecular único incluyen secuencias no aleatorias. También se proporcionan sistemas, aparatos y productos de programa informático para determinar una secuencia de interés implementando los métodos desvelados.
MX2022008045A 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). MX2022008045A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562153699P 2015-04-28 2015-04-28
US201562193469P 2015-07-16 2015-07-16
US201562269485P 2015-12-18 2015-12-18

Publications (1)

Publication Number Publication Date
MX2022008045A true MX2022008045A (es) 2022-07-27

Family

ID=55910388

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).
MX2022008045A MX2022008045A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).

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MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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US (3) US10844428B2 (es)
EP (4) EP3736341B1 (es)
JP (1) JP6685324B2 (es)
KR (1) KR102091312B1 (es)
CN (2) CN108138227B (es)
AU (3) AU2016256351B2 (es)
CA (2) CA3109403A1 (es)
DK (2) DK3289097T4 (es)
ES (2) ES2961338T3 (es)
FI (2) FI3289097T4 (es)
IL (3) IL285319B2 (es)
MX (3) MX2017013775A (es)
MY (1) MY181983A (es)
NZ (1) NZ736609A (es)
RU (1) RU2704286C2 (es)
SG (2) SG10202006185QA (es)
WO (1) WO2016176091A1 (es)
ZA (1) ZA201707231B (es)

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
ES2640776T3 (es) 2009-09-30 2017-11-06 Natera, Inc. Métodos para denominar de forma no invasiva ploidía prenatal
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
AU2011255641A1 (en) 2010-05-18 2012-12-06 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10179937B2 (en) 2014-04-21 2019-01-15 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
EP3363901B1 (en) 2012-02-17 2020-12-30 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
DK2828218T3 (da) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
IL269097B2 (en) 2012-09-04 2024-01-01 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN109457030B (zh) 2012-10-29 2022-02-18 约翰·霍普金斯大学 卵巢和子宫内膜癌的帕帕尼科拉乌测试
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3080298B1 (en) 2013-12-11 2018-10-31 AccuraGen Holdings Limited Methods for detecting rare sequence variants
EP3378952B1 (en) 2013-12-28 2020-02-05 Guardant Health, Inc. Methods and systems for detecting genetic variants
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
HK1259101A1 (zh) 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
PT3387152T (pt) 2015-12-08 2022-04-19 Twinstrand Biosciences Inc Adaptadores, métodos e composições melhorados para sequenciamento duplex
WO2017106768A1 (en) 2015-12-17 2017-06-22 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
JP6714709B2 (ja) * 2016-01-29 2020-06-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 核酸配列決定のための新規アダプターおよび使用法
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
ES3002715T3 (en) 2016-04-14 2025-03-07 Guardant Health Inc Methods for early detection of cancer
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018005811A1 (en) 2016-06-30 2018-01-04 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
WO2018119452A2 (en) 2016-12-22 2018-06-28 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
CN110741096B (zh) 2016-12-28 2023-07-14 奎斯特诊断投资有限责任公司 用于检测循环肿瘤dna的组合物和方法
CA3050247A1 (en) * 2017-01-18 2018-07-26 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018144159A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Capture probes using positive and negative strands for duplex sequencing
US10752946B2 (en) 2017-01-31 2020-08-25 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10968447B2 (en) * 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018148289A2 (en) * 2017-02-08 2018-08-16 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
EP3585889A1 (en) 2017-02-21 2020-01-01 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
WO2018183918A1 (en) * 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183942A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
WO2018204423A1 (en) 2017-05-01 2018-11-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP4443436A3 (en) 2017-05-08 2024-12-25 Illumina, Inc. Universal short adapters for indexing of polynucleotide
WO2018211497A1 (en) 2017-05-14 2018-11-22 Foresee Genomic Ltd Dna construct for sequencing and method for preparing the same
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
CN111032882A (zh) * 2017-06-20 2020-04-17 伊鲁米那股份有限公司 解决扩增反应中低效的方法和组合物
EP3642358A1 (en) * 2017-06-21 2020-04-29 Bluedot LLC Systems and methods for identification of nucleic acids in a sample
WO2019023924A1 (en) * 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES
KR102372572B1 (ko) 2017-08-04 2022-03-08 빌리언투원, 인크. 생물학적 표적과 연관된 정량화에서 표적 연관 분자를 이용한 서열분석 출력값 측정 및 분석
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
WO2019028462A1 (en) 2017-08-04 2019-02-07 Billiontoone, Inc. TARGET-ASSOCIATED MOLECULES FOR CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
EP3665308A1 (en) 2017-08-07 2020-06-17 The Johns Hopkins University Methods and materials for assessing and treating cancer
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
KR102878365B1 (ko) * 2017-10-09 2025-10-31 주식회사 마크로젠 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
CN107604046B (zh) * 2017-11-03 2021-08-24 上海交通大学 用于微量dna超低频突变检测的双分子自校验文库制备及杂交捕获的二代测序方法
CN118126816A (zh) * 2017-11-06 2024-06-04 伊鲁米那股份有限公司 核酸索引化技术
EP3707273B1 (en) * 2017-11-08 2024-04-10 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
KR102356323B1 (ko) * 2017-11-30 2022-01-26 일루미나, 인코포레이티드 서열 변이체 콜에 대한 검증방법 및 시스템
KR101967879B1 (ko) * 2017-11-30 2019-04-10 사회복지법인 삼성생명공익재단 핵산 서열분석에서 uid 핵산 서열의 순결도를 측정하는 방법
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
DK3735470T3 (da) 2018-01-05 2024-02-26 Billiontoone Inc Kvalitetskontroltemplates til sikring af validiteten af sekventeringsbaserede analyser
WO2019161244A1 (en) 2018-02-15 2019-08-22 Natera, Inc. Methods for isolating nucleic acids with size selection
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
GB2587939B (en) 2018-04-02 2023-06-14 Grail Llc Methylation markers and targeted methylation probe panels
EP3781714B1 (en) 2018-04-14 2026-01-07 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
EP3781713B1 (en) * 2018-04-16 2026-03-18 Memorial Sloan-Kettering Cancer Center Systems and methods for detecting cancer via cfdna screening
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) * 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CN108642173B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测slc26a4基因突变的方法和试剂盒
CN108486230B (zh) * 2018-05-18 2022-02-08 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的试剂盒及其制备方法
CN108753934B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种检测基因突变的方法、试剂盒及其制备方法
CN108841946B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测gjb2基因突变的方法，试剂盒及其制备方法
CN108531583B (zh) * 2018-05-18 2022-05-17 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的引物组合及检测方法
CN108949951B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种同时无创检测gjb2和slc26a4基因突变的方法和试剂盒
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN108949941A (zh) * 2018-06-25 2018-12-07 北京莲和医学检验所有限公司 低频突变检测方法、试剂盒和装置
CN110669823B (zh) * 2018-07-03 2022-05-24 中国医学科学院肿瘤医院 一种同时检测多种肝癌常见突变的ctDNA文库构建和测序数据分析方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
GB201810901D0 (en) * 2018-07-03 2018-08-15 Ucb Biopharma Sprl Method
KR20210059694A (ko) 2018-07-12 2021-05-25 트윈스트랜드 바이오사이언시스, 인코포레이티드 게놈 편집, 클론 팽창 및 연관된 분야를 규명하기 위한 방법 및 시약
EP3833776A4 (en) 2018-08-06 2022-04-27 Billiontoone, Inc. DILUTION MARKER FOR QUANTIFICATION OF BIOLOGICAL TARGETS
US11600360B2 (en) * 2018-08-20 2023-03-07 Microsoft Technology Licensing, Llc Trace reconstruction from reads with indeterminant errors
EP3844300A1 (en) 2018-08-28 2021-07-07 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
SG11202102954QA (en) * 2018-10-04 2021-04-29 Bluestar Genomics Inc Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample
EP3670670A1 (en) 2018-12-18 2020-06-24 Ricoh Company, Ltd. Nucleic acid analysis method, nucleic acid analysis program, and device for library preparation
CN109706219A (zh) * 2018-12-20 2019-05-03 臻和（北京）科技有限公司 构建测序文库的方法、试剂盒、上机方法及测序数据的拆分方法
US11473136B2 (en) * 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
EP3938505A4 (en) * 2019-02-25 2022-11-30 Twist Bioscience Corporation COMPOSITIONS AND METHODS FOR SEQUENCING THE NEXT GENERATION
WO2020226528A1 (ru) * 2019-05-08 2020-11-12 Общество с ограниченной ответственностью "ГЕНОТЕК ИТ" Способ определения кариотипа плода беременной женщины
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
CN110409001B (zh) * 2019-07-25 2022-11-15 北京贝瑞和康生物技术有限公司 一种构建捕获文库的方法和试剂盒
EP3795685A1 (en) * 2019-09-20 2021-03-24 Sophia Genetics S.A. Methods for dna library generation to facilitate the detection and reporting of low frequency variants
US10927409B1 (en) 2019-10-14 2021-02-23 Pioneer Hi-Bred International, Inc. Detection of sequences uniquely associated with a dna target region
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
BR112021018933A2 (pt) 2019-12-05 2022-06-21 Illumina Inc Detecção rápida de fusões genéticas
CN111073961A (zh) * 2019-12-20 2020-04-28 苏州赛美科基因科技有限公司 一种基因稀有突变的高通量检测方法
US20230129075A1 (en) * 2020-01-13 2023-04-27 St. Jude Children's Research Hospital Error suppression in genetic sequencing
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
EP3859012A1 (en) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
BR112022015909A2 (pt) 2020-02-14 2022-10-04 Univ Johns Hopkins Métodos e materiais para avaliação de ácidos nucleicos
CN111304288A (zh) * 2020-02-18 2020-06-19 江苏先声医学诊断有限公司 特异性分子标签umi组及其应用
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP3892737A1 (en) * 2020-04-09 2021-10-13 Takeda Vaccines, Inc. Qualitative and quantitative determination of single virus haplotypes in complex samples
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
MX2023001400A (es) 2020-09-11 2023-04-25 Illumina Cambridge Ltd Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla.
CN116438319A (zh) 2020-10-21 2023-07-14 Illumina公司 包含多个插入物的测序模板以及用于提高测序通量的组合物和方法
WO2022109207A2 (en) 2020-11-19 2022-05-27 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Massively paralleled multi-patient assay for pathogenic infection diagnosis and host physiology surveillance using nucleic acid sequencing
EP4259826A4 (en) * 2020-12-10 2024-09-04 Agilent Technologies, Inc. METHODS FOR SEQUENCING POLYNUCLEOTIDE FRAGMENTS FROM BOTH ENDS
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
EP4265721A4 (en) 2020-12-15 2024-11-13 Genodive Pharma Inc. METHOD FOR EVALUATING ADAPTER LIGATION EFFICIENCY IN A DNA SAMPLE SEQUENCE
CN112687339B (zh) * 2021-01-21 2021-12-14 深圳吉因加医学检验实验室 一种统计血浆dna片段测序数据中序列错误的方法和装置
EP4314328A1 (en) 2021-03-29 2024-02-07 Illumina, Inc. Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
MX2023010495A (es) 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
BR112023019945A2 (pt) 2021-03-31 2023-11-14 Illumina Cambridge Ltd Métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de erros
AU2022277902A1 (en) * 2021-05-19 2023-12-14 Illumina, Inc. Umi collapsing
CA3255101A1 (en) 2022-03-21 2023-09-28 Billion Toone, Inc. Counting of circulating methylated cell-free DNA molecules for treatment monitoring
CN117255857A (zh) * 2022-04-18 2023-12-19 京东方科技集团股份有限公司 接头、接头连接试剂及试剂盒和文库构建方法
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
CN120359304A (zh) * 2022-12-16 2025-07-22 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
TW202436625A (zh) * 2023-03-01 2024-09-16 體學生物科技股份有限公司 核酸分子的檢測方法、檢測套組及檢測卡匣
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
WO2025132779A2 (en) * 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme
WO2026039088A2 (en) 2024-06-03 2026-02-19 Esbiolab, Llc Excitation and detection methods for analyzing biomolecules
WO2025255512A1 (en) 2024-06-07 2025-12-11 Juno Therapeutics, Inc. Grna sequencing compositions and methods

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20030064366A1 (en) 2000-07-07 2003-04-03 Susan Hardin Real-time sequence determination
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040018520A1 (en) 2002-04-22 2004-01-29 James Thompson Trans-splicing enzymatic nucleic acid mediated biopharmaceutical and protein
JP3630414B2 (ja) 2002-05-21 2005-03-16 インターナショナル・ビジネス・マシーンズ・コーポレーション 塩基配列のクラスタ生成システム、塩基配列のクラスタ生成方法、該クラスタ生成方法を実行するためのプログラム、および該プログラムを記憶したコンピュータ可読な記録媒体、および塩基配列情報提供システム
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
EP1790202A4 (en) 2004-09-17 2013-02-20 Pacific Biosciences California APPARATUS AND METHOD FOR ANALYZING MOLECULES
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
ES2706227T3 (es) 2009-03-30 2019-03-27 Illumina Inc Análisis de expresión génica en células individuales
CN102656279A (zh) * 2009-12-17 2012-09-05 凯津公司 基于全基因组测序的限制性酶
AU2010343278B2 (en) * 2010-01-19 2015-05-21 Verinata Health, Inc. Simultaneous determination of aneuploidy and fetal fraction
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
US20110257031A1 (en) 2010-02-12 2011-10-20 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
WO2011139797A2 (en) 2010-04-27 2011-11-10 Spiral Genetics Inc. Method and system for analysis and error correction of biological sequences and inference of relationship for multiple samples
JP5516880B2 (ja) 2010-07-09 2014-06-11 独立行政法人産業技術総合研究所 配列解析装置、配列解析方法およびコンピュータプログラム
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
EP2702175B1 (en) * 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP2729580B1 (en) * 2011-07-08 2015-09-16 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
EP2563937A1 (en) * 2011-07-26 2013-03-06 Verinata Health, Inc Method for determining the presence or absence of different aneuploidies in a sample
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
AU2012327251A1 (en) 2011-10-27 2013-05-23 Verinata Health, Inc. Set membership testers for aligning nucleic acid samples
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20130267428A1 (en) 2012-02-10 2013-10-10 Washington University In St. Louis High throughput digital karyotyping for biome characterization
CA2867293C (en) 2012-03-13 2020-09-01 Abhijit Ajit PATEL Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
DK2828218T3 (da) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
EP2850211B1 (en) 2012-05-14 2021-09-08 iRepertoire, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
WO2013177214A2 (en) 2012-05-21 2013-11-28 Distributed Bio Inc Epitope focusing by variable effective antigen surface concentration
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
US10777301B2 (en) 2012-07-13 2020-09-15 Pacific Biosciences For California, Inc. Hierarchical genome assembly method using single long insert library
US20140024541A1 (en) 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
EP3795696B1 (en) 2013-03-15 2023-04-26 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
CN105593683B (zh) 2013-10-01 2018-11-30 考利达基因组股份有限公司 鉴定基因组中的变异的定相和连接方法
WO2015058052A1 (en) * 2013-10-18 2015-04-23 The Broad Institute Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
EP3378952B1 (en) 2013-12-28 2020-02-05 Guardant Health, Inc. Methods and systems for detecting genetic variants
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015179493A1 (en) 2014-05-23 2015-11-26 Centrillion Technology Holding Corporation Methods for generating and decoding barcodes
ES2968004T3 (es) 2014-07-30 2024-05-06 Harvard College Construcción de bibliotecas de sondas
ES2925014T3 (es) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
WO2016168351A1 (en) 2015-04-15 2016-10-20 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US20160370380A1 (en) 2015-06-16 2016-12-22 Pharmaseq, Inc. Combinatorial antibody diagnostic
CN105063208B (zh) 2015-08-10 2018-03-06 北京吉因加科技有限公司 一种血浆中游离的目标dna低频突变富集测序方法
PT3387152T (pt) 2015-12-08 2022-04-19 Twinstrand Biosciences Inc Adaptadores, métodos e composições melhorados para sequenciamento duplex
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
CN113151423B (zh) 2016-08-05 2024-09-10 生物辐射实验室股份有限公司 第二链引导
CA3050247A1 (en) 2017-01-18 2018-07-26 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018148289A2 (en) 2017-02-08 2018-08-16 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
ES2929281T3 (es) 2017-03-23 2022-11-28 Univ Washington Métodos para el enriquecimiento de secuencias de ácidos nucleicos dirigidos con aplicaciones para la secuenciación de ácidos nucleicos con corrección de errores
WO2018204423A1 (en) 2017-05-01 2018-11-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP4443436A3 (en) 2017-05-08 2024-12-25 Illumina, Inc. Universal short adapters for indexing of polynucleotide
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
EP3707273B1 (en) 2017-11-08 2024-04-10 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
RU2020130024A (ru) 2018-02-13 2022-03-14 Твинстранд Биосайенсис, Инк. Способы и реагенты для обнаружения и оценки генотоксичности
CA3093846A1 (en) 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
BR112020023296A2 (pt) 2018-05-16 2021-05-04 Twinstrand Biosciences, Inc. métodos e reagentes para resolver misturas de ácido nucleico e populações de células mistas e aplicações associadas
KR20210059694A (ko) 2018-07-12 2021-05-25 트윈스트랜드 바이오사이언시스, 인코포레이티드 게놈 편집, 클론 팽창 및 연관된 분야를 규명하기 위한 방법 및 시약
AU2019362855A1 (en) 2018-10-16 2021-05-20 Twinstrand Biosciences, Inc. Methods and reagents for efficient genotyping of large numbers of samples via pooling
CA3146435A1 (en) 2019-08-01 2021-02-04 Jesse J. SALK Methods and reagents for nucleic acid sequencing and associated applications

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