MX2022008045A - Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). - Google Patents

Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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Publication number
MX2022008045A
MX2022008045A MX2022008045A MX2022008045A MX2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A
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MX
Mexico
Prior art keywords
umis
sequences
unique molecular
dna fragments
sequenced dna
Prior art date
Application number
MX2022008045A
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English (en)
Inventor
Emrah Kostem
Sante Gnerre
Alex Aravanis
Byoungsok Jung
Zhihong Zhang
Xuyu Cai
Alex So
Frank J Steemers
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55910388&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2022008045(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2022008045A publication Critical patent/MX2022008045A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Las realizaciones desveladas se refieren a métodos, aparatos, sistemas y productos de programa informático para determinar secuencias de interés usando secuencias de índice molecular único (IMU) que son asociables de forma única a fragmentos polinucleotídicos individuales, que incluyen secuencias con bajas frecuencias alélicas y longitud de secuencia larga. En algunas implementaciones, los IMU incluyen tanto IMU físicos (exógenos), por ejemplo introducidos usando adaptadores en forma de Y, como IMU virtuales (endógenos) presentes en los fragmentos de ADN para secuenciar. En algunas implementaciones, las secuencias de índice molecular único incluyen secuencias no aleatorias. También se proporcionan sistemas, aparatos y productos de programa informático para determinar una secuencia de interés implementando los métodos desvelados.
MX2022008045A 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). MX2022008045A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562153699P 2015-04-28 2015-04-28
US201562193469P 2015-07-16 2015-07-16
US201562269485P 2015-12-18 2015-12-18

Publications (1)

Publication Number Publication Date
MX2022008045A true MX2022008045A (es) 2022-07-27

Family

ID=55910388

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).
MX2022008045A MX2022008045A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

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MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

Country Status (18)

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US (3) US10844428B2 (es)
EP (4) EP4700778A2 (es)
JP (1) JP6685324B2 (es)
KR (1) KR102091312B1 (es)
CN (2) CN113832139A (es)
AU (3) AU2016256351B2 (es)
CA (2) CA3109403A1 (es)
DK (2) DK3289097T4 (es)
ES (3) ES2961338T3 (es)
FI (2) FI3736341T3 (es)
IL (3) IL285319B2 (es)
MX (3) MX2017013775A (es)
MY (1) MY181983A (es)
NZ (1) NZ736609A (es)
RU (1) RU2704286C2 (es)
SG (2) SG11201708859XA (es)
WO (1) WO2016176091A1 (es)
ZA (1) ZA201707231B (es)

Families Citing this family (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
EP2473638B1 (en) 2009-09-30 2017-08-09 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2656263B1 (en) 2010-12-22 2019-11-06 Natera, Inc. Methods for non-invasive prenatal paternity testing
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
HUE053360T2 (hu) 2012-02-17 2021-06-28 Hutchinson Fred Cancer Res Kompozíciók és eljárások mutációk pontos azonosítására
ES2828661T3 (es) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
DE202013012824U1 (de) 2012-09-04 2020-03-10 Guardant Health, Inc. Systeme zum Erfassen von seltenen Mutationen und einer Kopienzahlvariation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR20240025725A (ko) 2013-12-11 2024-02-27 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
CN113774132A (zh) 2014-04-21 2021-12-10 纳特拉公司 检测染色体片段中的突变和倍性
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
MX2018004284A (es) 2015-10-09 2018-05-16 Accuragen Holdings Ltd Metodos y composiciones para el enriquecimiento de productos de amplificacion.
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
PL3387152T3 (pl) 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
CN117174167A (zh) 2015-12-17 2023-12-05 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
CN108474026A (zh) * 2016-01-29 2018-08-31 豪夫迈·罗氏有限公司 用于核酸测序的新的衔接子和使用方法
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
DK3443119T3 (da) 2016-04-15 2022-05-23 Natera Inc Fremgangsmåde til bestemmelse af lunge cancer
CN109511265B (zh) * 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
EP4257701A3 (en) 2016-06-30 2023-12-20 Grail, LLC Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
WO2018119452A2 (en) 2016-12-22 2018-06-28 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
BR112019013391A2 (pt) 2016-12-28 2020-03-03 Quest Diagnostics Investments Llc Adaptador de ácido nucleico, e, método para detecção de uma mutação em uma molécula de dna circulante tumoral (ctdna) de fita dupla.
US10844429B2 (en) * 2017-01-18 2020-11-24 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144159A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Capture probes using positive and negative strands for duplex sequencing
US20180223350A1 (en) * 2017-02-08 2018-08-09 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
WO2018156418A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
WO2018183918A1 (en) * 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
US11584958B2 (en) * 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
CN110832087B (zh) 2017-05-08 2025-03-18 伊鲁米那股份有限公司 用于多核苷酸样品的索引的通用短衔接子
WO2018211497A1 (en) 2017-05-14 2018-11-22 Foresee Genomic Ltd Dna construct for sequencing and method for preparing the same
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11702654B2 (en) * 2017-06-20 2023-07-18 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
US12040053B2 (en) 2017-06-21 2024-07-16 Bluedot, Llc Methods for generating sequencer-specific nucleic acid barcodes that reduce demultiplexing errors
JP6998404B2 (ja) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
WO2019028462A1 (en) 2017-08-04 2019-02-07 Billiontoone, Inc. TARGET-ASSOCIATED MOLECULES FOR CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
IL272399B2 (en) 2017-08-04 2025-03-01 Billiontoone Inc Sequencing output determination and analysis with target-associated molecules in quantification associated with biological targets
CN120400338A (zh) 2017-08-07 2025-08-01 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019074960A1 (en) * 2017-10-09 2019-04-18 uBiome, Inc. SEQUENCING A SINGLE MOLECULE AND UNIQUE MOLECULAR IDENTIFIERS FOR CHARACTERIZING NUCLEIC ACID SEQUENCES
CN107604046B (zh) * 2017-11-03 2021-08-24 上海交通大学 用于微量dna超低频突变检测的双分子自校验文库制备及杂交捕获的二代测序方法
EP4289996B1 (en) * 2017-11-06 2026-02-11 Illumina Inc. Nucleic acid indexing techniques
ES2977793T3 (es) 2017-11-08 2024-08-30 Twinstrand Biosciences Inc Reactivos y adaptadores para la secuenciación de ácidos nucleicos y métodos para fabricar dichos reactivos y adaptadores
IL271235B2 (en) * 2017-11-30 2024-12-01 Illumina Inc Validation methods and systems for detecting sequence variants
KR101967879B1 (ko) * 2017-11-30 2019-04-10 사회복지법인 삼성생명공익재단 핵산 서열분석에서 uid 핵산 서열의 순결도를 측정하는 방법
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
SG11202006110UA (en) 2018-01-05 2020-07-29 Billiontoone Inc Quality control templates for ensuring validity of sequencing-based assays
WO2019161244A1 (en) 2018-02-15 2019-08-22 Natera, Inc. Methods for isolating nucleic acids with size selection
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
AU2019249422B2 (en) 2018-04-02 2025-09-25 Grail, Inc. Methylation markers and targeted methylation probe panels
DK3781714T3 (da) 2018-04-14 2026-03-30 Natera Inc Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna
WO2019204208A1 (en) * 2018-04-16 2019-10-24 Memorial Sloan Kettering Cancer Center SYSTEMS AND METHODS FOR DETECTING CANCER VIA cfDNA SCREENING
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
EP3788170B1 (en) * 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CN108486230B (zh) * 2018-05-18 2022-02-08 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的试剂盒及其制备方法
CN108753934B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种检测基因突变的方法、试剂盒及其制备方法
CN108949951B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种同时无创检测gjb2和slc26a4基因突变的方法和试剂盒
CN108642173B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测slc26a4基因突变的方法和试剂盒
CN108531583B (zh) * 2018-05-18 2022-05-17 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的引物组合及检测方法
CN108841946B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测gjb2基因突变的方法，试剂盒及其制备方法
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN108949941A (zh) * 2018-06-25 2018-12-07 北京莲和医学检验所有限公司 低频突变检测方法、试剂盒和装置
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN110669823B (zh) * 2018-07-03 2022-05-24 中国医学科学院肿瘤医院 一种同时检测多种肝癌常见突变的ctDNA文库构建和测序数据分析方法
GB201810901D0 (en) * 2018-07-03 2018-08-15 Ucb Biopharma Sprl Method
WO2020014693A1 (en) 2018-07-12 2020-01-16 Twinstrand Biosciences, Inc. Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
WO2020033425A1 (en) 2018-08-06 2020-02-13 Billiontoone, Inc. Dilution tagging for quantification of biological targets
US11600360B2 (en) * 2018-08-20 2023-03-07 Microsoft Technology Licensing, Llc Trace reconstruction from reads with indeterminant errors
EP3844300A1 (en) * 2018-08-28 2021-07-07 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
IL281741B2 (en) 2018-09-27 2026-04-01 Grail Inc Methylation markers and a targeted methylation detection panel
MX2021003847A (es) * 2018-10-04 2021-05-27 Bluestar Genomics Inc Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.
EP3670670A1 (en) 2018-12-18 2020-06-24 Ricoh Company, Ltd. Nucleic acid analysis method, nucleic acid analysis program, and device for library preparation
CN109706219A (zh) * 2018-12-20 2019-05-03 臻和（北京）科技有限公司 构建测序文库的方法、试剂盒、上机方法及测序数据的拆分方法
US11473136B2 (en) * 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
CN120099139A (zh) 2019-02-14 2025-06-06 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2020176362A1 (en) * 2019-02-25 2020-09-03 Twist Bioscience Corporation Compositions and methods for next generation sequencing
WO2020226528A1 (ru) * 2019-05-08 2020-11-12 Общество с ограниченной ответственностью "ГЕНОТЕК ИТ" Способ определения кариотипа плода беременной женщины
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN110409001B (zh) * 2019-07-25 2022-11-15 北京贝瑞和康生物技术有限公司 一种构建捕获文库的方法和试剂盒
EP3795685A1 (en) * 2019-09-20 2021-03-24 Sophia Genetics S.A. Methods for dna library generation to facilitate the detection and reporting of low frequency variants
US10927409B1 (en) * 2019-10-14 2021-02-23 Pioneer Hi-Bred International, Inc. Detection of sequences uniquely associated with a dna target region
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
BR112021018933A2 (pt) 2019-12-05 2022-06-21 Illumina Inc Detecção rápida de fusões genéticas
CN111073961A (zh) * 2019-12-20 2020-04-28 苏州赛美科基因科技有限公司 一种基因稀有突变的高通量检测方法
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2021146304A1 (en) * 2020-01-13 2021-07-22 St. Jude Children's Research Hospital Error suppression in genetic sequencing
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
EP3859012A1 (en) * 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
IL295297A (en) 2020-02-14 2022-10-01 Univ Johns Hopkins Methods and materials for assessing nucleic acids
CN111304288A (zh) * 2020-02-18 2020-06-19 江苏先声医学诊断有限公司 特异性分子标签umi组及其应用
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
EP3892737A1 (en) * 2020-04-09 2021-10-13 Takeda Vaccines, Inc. Qualitative and quantitative determination of single virus haplotypes in complex samples
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
IL300983A (en) 2020-09-11 2023-04-01 Illumina Cambridge Ltd Methods for enriching a target sequence from a sequence library using hairpin adapters
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
US20230374592A1 (en) 2020-11-19 2023-11-23 The Usa, As Represented By The Secretary, Dept. Of Health And Human Services Massively paralleled multi-patient assay for pathogenic infection diagnosis and host physiology surveillance using nucleic acid sequencing
EP4259826A4 (en) * 2020-12-10 2024-09-04 Agilent Technologies, Inc. METHODS FOR SEQUENCING POLYNUCLEOTIDE FRAGMENTS FROM BOTH ENDS
KR20230121076A (ko) 2020-12-15 2023-08-17 제노다이브 파마 가부시키가이샤 Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
CN112687339B (zh) * 2021-01-21 2021-12-14 深圳吉因加医学检验实验室 一种统计血浆dna片段测序数据中序列错误的方法和装置
CA3214282A1 (en) 2021-03-29 2022-10-06 Illumina, Inc. Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
MX2023010495A (es) 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
KR20230164668A (ko) 2021-03-31 2023-12-04 일루미나, 인코포레이티드 오류 수정을 위한 고유 분자 식별자를 이용한 트랜스포존-기반 기술을 사용하는 방향성 태그먼트화 시퀀싱 라이브러리 제조 방법
US20220392575A1 (en) * 2021-05-19 2022-12-08 Illumina, Inc. Umi collapsing
AU2023240345A1 (en) 2022-03-21 2024-10-10 Billion Toone, Inc. Molecule counting of methylated cell-free dna for treatment monitoring
US20250297406A1 (en) * 2022-04-18 2025-09-25 Chengdu Boe Optoelectronics Technology Co., Ltd. Adapters, adapter ligation reagent, kit, method for constructing dna library and method for sequencing gene
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
CN120359304A (zh) * 2022-12-16 2025-07-22 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
CN118581200A (zh) * 2023-03-01 2024-09-03 体学生物科技股份有限公司 核酸分子的检测方法、检测套组及检测卡匣
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
CN116913403B (zh) * 2023-06-29 2026-04-28 中移(成都)信息通信科技有限公司 数据预处理方法、装置、电子设备及存储介质
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
WO2025132779A2 (en) * 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme
WO2026039088A2 (en) 2024-06-03 2026-02-19 Esbiolab, Llc Excitation and detection methods for analyzing biomolecules
WO2025255512A1 (en) 2024-06-07 2025-12-11 Juno Therapeutics, Inc. Grna sequencing compositions and methods

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
EP1591541B1 (en) 1997-04-01 2012-02-15 Illumina Cambridge Limited Method of nucleic acid sequencing
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040018520A1 (en) 2002-04-22 2004-01-29 James Thompson Trans-splicing enzymatic nucleic acid mediated biopharmaceutical and protein
JP3630414B2 (ja) 2002-05-21 2005-03-16 インターナショナル・ビジネス・マシーンズ・コーポレーション 塩基配列のクラスタ生成システム、塩基配列のクラスタ生成方法、該クラスタ生成方法を実行するためのプログラム、および該プログラムを記憶したコンピュータ可読な記録媒体、および塩基配列情報提供システム
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP3002337B1 (en) 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
US8932812B2 (en) * 2009-12-17 2015-01-13 Keygene N.V. Restriction enzyme based whole genome sequencing
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
AU2010343277B2 (en) * 2010-01-19 2015-05-28 Verinata Health, Inc. Method for determining copy number variations
US20110257031A1 (en) 2010-02-12 2011-10-20 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
US20120053845A1 (en) 2010-04-27 2012-03-01 Jeremy Bruestle Method and system for analysis and error correction of biological sequences and inference of relationship for multiple samples
JP5516880B2 (ja) 2010-07-09 2014-06-11 独立行政法人産業技術総合研究所 配列解析装置、配列解析方法およびコンピュータプログラム
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
AU2012242847B2 (en) * 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
WO2012149042A2 (en) * 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
AU2012281242B2 (en) * 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
CA2840418C (en) * 2011-07-26 2019-10-29 Verinata Health, Inc. Method for determining the presence or absence of different aneuploidies in a sample
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
WO2013062856A1 (en) 2011-10-27 2013-05-02 Verinata Health, Inc. Set membership testers for aligning nucleic acid samples
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20130267428A1 (en) 2012-02-10 2013-10-10 Washington University In St. Louis High throughput digital karyotyping for biome characterization
EP2825675B1 (en) 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
ES2828661T3 (es) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria
EP2850211B1 (en) 2012-05-14 2021-09-08 iRepertoire, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
US9884893B2 (en) 2012-05-21 2018-02-06 Distributed Bio, Inc. Epitope focusing by variable effective antigen surface concentration
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US10777301B2 (en) 2012-07-13 2020-09-15 Pacific Biosciences For California, Inc. Hierarchical genome assembly method using single long insert library
US20140024541A1 (en) 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
EP3553175B1 (en) 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
CN113337604A (zh) 2013-03-15 2021-09-03 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
CN109599148B (zh) 2013-10-01 2022-03-25 深圳华大智造科技有限公司 鉴定基因组中的变异的定相和连接方法
WO2015058052A1 (en) * 2013-10-18 2015-04-23 The Broad Institute Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US20170233727A1 (en) 2014-05-23 2017-08-17 Centrillion Technology Holdings Corporation Methods for generating and decoding barcodes
CN107075545A (zh) 2014-07-30 2017-08-18 哈佛学院院长及董事 探针文库构建
ES2925014T3 (es) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
WO2016168351A1 (en) 2015-04-15 2016-10-20 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US20160370380A1 (en) 2015-06-16 2016-12-22 Pharmaseq, Inc. Combinatorial antibody diagnostic
CN105063208B (zh) 2015-08-10 2018-03-06 北京吉因加科技有限公司 一种血浆中游离的目标dna低频突变富集测序方法
PL3387152T3 (pl) 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
US10844429B2 (en) 2017-01-18 2020-11-24 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
US20180223350A1 (en) 2017-02-08 2018-08-09 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
US11479807B2 (en) 2017-03-23 2022-10-25 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
CN110832087B (zh) 2017-05-08 2025-03-18 伊鲁米那股份有限公司 用于多核苷酸样品的索引的通用短衔接子
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
ES2977793T3 (es) 2017-11-08 2024-08-30 Twinstrand Biosciences Inc Reactivos y adaptadores para la secuenciación de ácidos nucleicos y métodos para fabricar dichos reactivos y adaptadores
MX2020008472A (es) 2018-02-13 2020-11-11 Twinstrand Biosciences Inc Metodos y reactivos para detectar y evaluar la genotoxicidad.
JP2021515579A (ja) 2018-03-15 2021-06-24 ツインストランド・バイオサイエンシズ・インコーポレイテッドＴｗｉｎｓｔｒａｎｄ Ｂｉｏｓｃｉｅｎｃｅｓ， Ｉｎｃ． 配列決定用途および他の核酸物質インテロゲーションのための核酸物質を濃縮するための方法および試薬
KR102873073B1 (ko) 2018-05-16 2025-10-17 트윈스트랜드 바이오사이언시스, 인코포레이티드 핵산 혼합물 및 혼합된 세포 집단을 해상하기 위한 방법 및 시약 및 관련 응용
WO2020014693A1 (en) 2018-07-12 2020-01-16 Twinstrand Biosciences, Inc. Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
CA3115819A1 (en) 2018-10-16 2020-04-23 Twinstrand Biosciences, Inc. Methods and reagents for efficient genotyping of large numbers of samples via pooling
EP4007818A4 (en) 2019-08-01 2023-09-20 Twinstrand Biosciences, Inc. METHODS AND REAGENTS FOR SEQUENCING NUCLEIC ACIDS AND RELATED APPLICATIONS

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AU2022200179B2 (en) 2025-05-22
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