MX2021015842A - Polipeptido variante de dihidrodipicolinato reductasa y procedimiento de produccion de l-treonina mediante el uso del mismo. - Google Patents

Polipeptido variante de dihidrodipicolinato reductasa y procedimiento de produccion de l-treonina mediante el uso del mismo.

Info

Publication number
MX2021015842A
MX2021015842A MX2021015842A MX2021015842A MX2021015842A MX 2021015842 A MX2021015842 A MX 2021015842A MX 2021015842 A MX2021015842 A MX 2021015842A MX 2021015842 A MX2021015842 A MX 2021015842A MX 2021015842 A MX2021015842 A MX 2021015842A
Authority
MX
Mexico
Prior art keywords
threonine
producing
same
modified polypeptide
dihydrodipicolinate reductase
Prior art date
Application number
MX2021015842A
Other languages
English (en)
Inventor
Kwang Woo Lee
Imsang Lee
Seung-Ju Son
Su Yon Kwon
Mina Baek
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of MX2021015842A publication Critical patent/MX2021015842A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01026—Dihydrodipicolinate reductase (1.3.1.26)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01016—Diaminopimelate dehydrogenase (1.4.1.16)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
    • C12Y117/01—Oxidoreductases acting on CH or CH2 groups (1.17) with NAD+ or NADP+ as acceptor (1.17.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0102—Diaminopimelate decarboxylase (4.1.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/03—Amine-lyases (4.3.3)
    • C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente divulgación se refiere a un polipéptido variante que tiene actividad de dihidrodipicolinato reductasa atenuada y a un procedimiento de producción de L-treonina mediante el uso del mismo.
MX2021015842A 2019-09-26 2020-08-06 Polipeptido variante de dihidrodipicolinato reductasa y procedimiento de produccion de l-treonina mediante el uso del mismo. MX2021015842A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020190119159A KR102126951B1 (ko) 2019-09-26 2019-09-26 디하이드로디피콜린산 리덕타제 변이형 폴리펩티드 및 이를 이용한 l-쓰레오닌 생산방법
PCT/KR2020/010419 WO2021060696A1 (ko) 2019-09-26 2020-08-06 디하이드로디피콜린산 리덕타제 변이형 폴리펩티드 및 이를 이용한 l-쓰레오닌 생산방법

Publications (1)

Publication Number Publication Date
MX2021015842A true MX2021015842A (es) 2022-02-03

Family

ID=71136862

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021015842A MX2021015842A (es) 2019-09-26 2020-08-06 Polipeptido variante de dihidrodipicolinato reductasa y procedimiento de produccion de l-treonina mediante el uso del mismo.

Country Status (12)

Country Link
US (1) US20220356480A1 (es)
EP (1) EP3960851A4 (es)
JP (1) JP7311634B2 (es)
KR (1) KR102126951B1 (es)
CN (1) CN114729338B (es)
AR (1) AR120028A1 (es)
AU (1) AU2020356063B2 (es)
BR (1) BR112021025705A2 (es)
MX (1) MX2021015842A (es)
MY (1) MY197654A (es)
WO (1) WO2021060696A1 (es)
ZA (1) ZA202109712B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102126951B1 (ko) * 2019-09-26 2020-06-26 씨제이제일제당 주식회사 디하이드로디피콜린산 리덕타제 변이형 폴리펩티드 및 이를 이용한 l-쓰레오닌 생산방법
KR102635860B1 (ko) 2021-04-20 2024-02-13 씨제이제일제당 주식회사 L-아미노산을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아미노산의 생산 방법
KR20230131654A (ko) 2022-03-07 2023-09-14 씨제이제일제당 (주) 변이형 l-쓰레오닌 배출 단백질 및 이를 이용한 l-쓰레오닌 생산 방법
KR20230131653A (ko) 2022-03-07 2023-09-14 씨제이제일제당 (주) 변이형 l-쓰레오닌 배출 단백질 및 이를 이용한 l-쓰레오닌 생산 방법

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0159812B1 (ko) 1995-12-20 1998-11-16 손경식 코리네박테리움 글루타미컴 씨에이치 77 및 이 균주를 이용한 l-라이신의 제조 방법
JP4168463B2 (ja) * 1996-12-05 2008-10-22 味の素株式会社 Ｌ−リジンの製造法
ATE411378T1 (de) * 2000-01-21 2008-10-15 Ajinomoto Kk Verfahren zur herstellung von l-lysin
JP2005227977A (ja) 2004-02-12 2005-08-25 Fuji Heavy Ind Ltd 車販売支援システム、在庫管理サーバ、車販売支援プログラム及び車販売支援方法
DE102004035052A1 (de) * 2004-07-20 2006-02-16 Basf Ag Mikroorganismen zur Herstellung von schwefelhaltigen Verbindungen
JP5048996B2 (ja) 2006-11-10 2012-10-17 昭和電工株式会社 加工性に優れた耐摩耗性アルミニウム合金材およびその製造方法
CN101573447A (zh) * 2006-12-29 2009-11-04 赢创德固赛有限责任公司 具有降低的产物抑制作用的甲硫氨酸合酶
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100858913B1 (ko) * 2007-03-09 2008-09-17 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
RU2535973C2 (ru) * 2010-06-15 2014-12-20 Пайк Кванг Индастриал Ко., Лтд. Способ продуцирования аминокислот семейства аспартата с использованием микроорганизмов
KR101485222B1 (ko) * 2012-10-05 2015-01-22 상지대학교산학협력단 ｄａｐＡ 활성 조절을 통해 Ｌ-쓰레오닌 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 Ｌ-쓰레오닌 생산방법
US10889842B2 (en) * 2014-01-16 2021-01-12 Calysta, Inc. Microorganisms for the enhanced production of amino acids and related methods
KR101804017B1 (ko) * 2015-10-23 2017-12-04 씨제이제일제당 (주) Ｌ-쓰레오닌을 생산하는 재조합 미생물 및 이를 이용하여 ｌ-쓰레오닌을 생산하는 방법
CN110741091A (zh) * 2017-05-19 2020-01-31 齐默尔根公司 增加nadph的生物合成途径的基因组工程化
CN107916246A (zh) * 2017-11-17 2018-04-17 江南大学 遗传改造谷氨酸棒杆菌中dhdpr提高赖氨酸产量的方法
KR102233376B1 (ko) * 2019-09-26 2021-03-30 씨제이제일제당 주식회사 메조 디아미노피멜레이트 디하이드로게네이즈 변이형 폴리펩타이드 및 이를 이용한 l-쓰레오닌 생산방법
KR102126951B1 (ko) * 2019-09-26 2020-06-26 씨제이제일제당 주식회사 디하이드로디피콜린산 리덕타제 변이형 폴리펩티드 및 이를 이용한 l-쓰레오닌 생산방법

Also Published As

Publication number Publication date
BR112021025705A2 (pt) 2022-05-03
AU2020356063B2 (en) 2024-02-29
ZA202109712B (en) 2022-08-31
JP2022534403A (ja) 2022-07-29
US20220356480A1 (en) 2022-11-10
AR120028A1 (es) 2022-01-26
AU2020356063A1 (en) 2022-01-27
CN114729338B (zh) 2024-01-26
EP3960851A4 (en) 2022-08-31
MY197654A (en) 2023-06-30
JP7311634B2 (ja) 2023-07-19
CN114729338A (zh) 2022-07-08
WO2021060696A1 (ko) 2021-04-01
KR102126951B1 (ko) 2020-06-26
EP3960851A1 (en) 2022-03-02

Similar Documents

Publication Publication Date Title
MX2021015842A (es) Polipeptido variante de dihidrodipicolinato reductasa y procedimiento de produccion de l-treonina mediante el uso del mismo.
MX2020006106A (es) Polipeptido modificado con actividad atenuada de citrato sintasa y procedimiento para producir l-aminoacido utilizando el mismo.
MX2022006991A (es) Arn guía antisentido con región funcional añadida para editar arn blanco.
MY191029A (en) Novel polypeptide and method of producing imp using the same
PH12020550529A1 (en) Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine
MX2021007474A (es) Homoserina deshidrogenasa modificada y procedimiento para la produccion de homoserina o l-aminoacido derivado de homoserina mediante el uso de la misma.
MX2019011759A (es) Composicion para producir tagatosa y procedimiento de produccion de tagatosa usando el mismo.
MX2021000685A (es) Novedosa variante de proteina exportadora de l-triptofano y procedimiento para producir l-triptofano utilizando el mismo.
MX2020007591A (es) Homoserina deshidrogenasa modificada y procedimiento de produccion de homoserina o l-aminoacido derivado de homoserina mediante el uso de la misma.
MX2021014853A (es) Variante de proteina de exportacion de l-treonina y procedimiento para la produccion de l-treonina mediante el uso de la misma.
MX2020007924A (es) Novedoso exportador de l-triptofano y procedimiento de produccion de l-triptofano mediante el uso del mismo.
MX2017016152A (es) Modelado de fibras de queratina mediante el uso de compuestos de dialdehido.
MX2019011758A (es) Composicion para producir tagatosa y procedimiento de produccion de tagatosa usando la misma.
MX2021012642A (es) Nutricion en los primeros a?os.
MX2021005180A (es) Variante proteica receptora de ampc y procedimiento de produccion de l-aminoacido mediante el uso de la misma.
JOP20200295A1 (ar) أجسام أحادية النوعية ومتعددة النوعية مضادة لـ tmeff2 المضاد واستخداماتها
PH12018502082A1 (en) Method for producing protein
PH12019500096A1 (en) Leuconostoc mesenteroides cjlm119 strain producing reduced amount of gas, and kimchi production method using same
MX2022006073A (es) Metodos para utilizar anticuerpos anti-trem2.
MX2021012296A (es) Microorganismo que produce l-aminoacido y procedimiento de produccion de l-aminoacido mediante el uso del mismo.
PH12020550834A1 (en) Fermented soybean composition and method for producing fermented soybean composition
MY195400A (en) Microorganism Having Enhanced L-Threonine Producing Ability and Method for Producing Threonine using the same
MX2022006670A (es) Region fc de anticuerpo teniendo mayor afinidad de union al fcyriib.
MX2022004952A (es) Cepa de levadura productora de glutation y procedimiento de produccion de glutation mediante el uso de la misma.
MX2019009214A (es) Microorganismo del genero corynebacterium que produce 5'-xantosina monofosfato y procedimiento de preparacion de 5'-xantosina monofosfato mediante el uso del mismo.