MX2021012677A - Identificacion de proteinas de celulas huesped. - Google Patents

Identificacion de proteinas de celulas huesped.

Info

Publication number
MX2021012677A
MX2021012677A MX2021012677A MX2021012677A MX2021012677A MX 2021012677 A MX2021012677 A MX 2021012677A MX 2021012677 A MX2021012677 A MX 2021012677A MX 2021012677 A MX2021012677 A MX 2021012677A MX 2021012677 A MX2021012677 A MX 2021012677A
Authority
MX
Mexico
Prior art keywords
host
identification
host cell
cell proteins
protein
Prior art date
Application number
MX2021012677A
Other languages
English (en)
Inventor
Ning Li
I- Hsuan Chen
Hui Xiao
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2021012677A publication Critical patent/MX2021012677A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para identificar proteínas de la célula huésped en una matriz de muestra. Los métodos comprenden: disociar la proteína de la célula huésped de la proteína de interés; filtrar la proteína de la célula huésped con un filtro de corte de peso molecular e identificar la proteína de la célula huésped, preferentemente con espectrometría de masas.
MX2021012677A 2019-04-17 2020-04-16 Identificacion de proteinas de celulas huesped. MX2021012677A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962835065P 2019-04-17 2019-04-17
PCT/US2020/028458 WO2020214777A1 (en) 2019-04-17 2020-04-16 Identification of host cell proteins

Publications (1)

Publication Number Publication Date
MX2021012677A true MX2021012677A (es) 2021-11-12

Family

ID=70779848

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021012677A MX2021012677A (es) 2019-04-17 2020-04-16 Identificacion de proteinas de celulas huesped.

Country Status (13)

Country Link
US (1) US20200333353A1 (es)
EP (1) EP3956668A1 (es)
JP (1) JP2022528807A (es)
KR (1) KR20210153102A (es)
CN (1) CN114144672A (es)
AU (1) AU2020257220A1 (es)
BR (1) BR112021020378A2 (es)
CA (1) CA3136813A1 (es)
EA (1) EA202192850A1 (es)
IL (1) IL287246A (es)
MX (1) MX2021012677A (es)
SG (1) SG11202111200QA (es)
WO (1) WO2020214777A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117813504A (zh) * 2021-06-04 2024-04-02 基因泰克公司 对蛋白质样品中的残留宿主细胞蛋白质的鉴定和定量
CN114034791A (zh) * 2021-11-09 2022-02-11 谱天（天津）生物科技有限公司 一种利用聚苯乙烯类材料提高蛋白质和或肽段组质谱鉴定数的方法
WO2023164145A1 (en) * 2022-02-25 2023-08-31 Regeneron Pharmaceuticals, Inc. Size exclusion chromatography for characterizing host cell proteins
KR102629162B1 (ko) 2022-11-04 2024-01-29 한국기초과학지원연구원 바이오의약품 품질관리를 위한 잔류 숙주세포 단백질 정량 분석 방법 및 시스템
CN117169519B (zh) * 2023-10-26 2024-01-30 艾康生物技术(杭州)有限公司 用于检测样本中tt3和/或tt4的解离剂和试剂盒

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
JP4831708B2 (ja) * 2007-10-02 2011-12-07 学校法人慶應義塾 タンパク質試料の調製方法
FR2923610B1 (fr) * 2007-11-14 2009-11-27 Galderma Res & Dev Methode non-invasive de recueil de donnees biologiques pour l'etablissement d'un diagnostic d'une pathologie cutanee.
WO2016106291A1 (en) * 2014-12-22 2016-06-30 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
US20190169675A1 (en) * 2016-08-12 2019-06-06 Lonza Ltd Proteomic analysis of host cell proteins

Also Published As

Publication number Publication date
CA3136813A1 (en) 2020-10-22
EP3956668A1 (en) 2022-02-23
IL287246A (en) 2021-12-01
WO2020214777A1 (en) 2020-10-22
US20200333353A1 (en) 2020-10-22
KR20210153102A (ko) 2021-12-16
AU2020257220A1 (en) 2021-11-11
BR112021020378A2 (pt) 2021-12-07
EA202192850A1 (ru) 2022-01-26
JP2022528807A (ja) 2022-06-15
CN114144672A (zh) 2022-03-04
SG11202111200QA (en) 2021-11-29

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