WO2009145532A3 - 비젤방식의 2차원 단백질분리용 다중채널장치 - Google Patents

비젤방식의 2차원 단백질분리용 다중채널장치 Download PDF

Info

Publication number
WO2009145532A3
WO2009145532A3 PCT/KR2009/002742 KR2009002742W WO2009145532A3 WO 2009145532 A3 WO2009145532 A3 WO 2009145532A3 KR 2009002742 W KR2009002742 W KR 2009002742W WO 2009145532 A3 WO2009145532 A3 WO 2009145532A3
Authority
WO
WIPO (PCT)
Prior art keywords
isolation
protein
proteins
channel
stage
Prior art date
Application number
PCT/KR2009/002742
Other languages
English (en)
French (fr)
Other versions
WO2009145532A2 (ko
Inventor
문명희
김기훈
Original Assignee
연세대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 연세대학교 산학협력단 filed Critical 연세대학교 산학협력단
Priority to US12/531,281 priority Critical patent/US8298394B2/en
Publication of WO2009145532A2 publication Critical patent/WO2009145532A2/ko
Publication of WO2009145532A3 publication Critical patent/WO2009145532A3/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C07K1/285—Isoelectric focusing multi dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/0015—Field flow fractionation characterised by driving force
    • G01N2030/0025—Field flow fractionation characterised by driving force cross flow FFF
    • G01N2030/003—Asymmetrical flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

본 발명은 비젤방식의 2차원 단백질분리용 다중채널장치에 관한 것으로, 보다 상세하게는 단백질 시료를 pI 별로 1차 분리하는 등전집중부 및 1차로 분리된 단백질을 2차로 분자량별로 분리하는 흐름장흐름분획부를 함유하는 평판형 채널이 하나 이상 병렬구로 연결되어, 다중채널에서 단백질의 동시 분리가 가능하고, 단백질 분리속도가 향상되며, 채널부피 증가로 인한 시료 주입의 한계를 극복할 수 있는 비젤방식의 2차원 단백질분리용 다중채널장치에 관한 것이다. 본 발명에 따르면, 단백질을 pI와 분자량의 크기 순서에 따라 분리할 수 있으며, 단백질 분리공정에 따른 단백질의 변성이 일어나지 않으며, 등전점 분리에 사용되는 양쪽성 전해질의 자동제거가 가능하며, 분리된 단백질을 별도로 검출하여 나노유속 액체크로마토그래피-전자분무이온화-탠덤질량분석법을 적용함으로써 단백질 식별이 가능하다.
PCT/KR2009/002742 2008-05-26 2009-05-25 비젤방식의 2차원 단백질분리용 다중채널장치 WO2009145532A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/531,281 US8298394B2 (en) 2008-05-26 2009-05-25 Non-gel based two-dimensional protein separation multi-channel devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080048842A KR100958165B1 (ko) 2008-05-26 2008-05-26 비젤방식의 2차원 단백질분리용 다중채널장치
KR10-2008-0048842 2008-05-26

Publications (2)

Publication Number Publication Date
WO2009145532A2 WO2009145532A2 (ko) 2009-12-03
WO2009145532A3 true WO2009145532A3 (ko) 2010-03-04

Family

ID=41377758

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/002742 WO2009145532A2 (ko) 2008-05-26 2009-05-25 비젤방식의 2차원 단백질분리용 다중채널장치

Country Status (3)

Country Link
US (1) US8298394B2 (ko)
KR (1) KR100958165B1 (ko)
WO (1) WO2009145532A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014028173A1 (en) * 2012-08-15 2014-02-20 Array Bridge Inc. Methods for protein purification and analysis
WO2015051343A1 (en) * 2013-10-03 2015-04-09 Northwestern University High throughput mass spectrometric analysis of proteome samples
KR101699520B1 (ko) * 2015-06-10 2017-01-24 동국대학교 산학협력단 단백질 분리 장치 및 이를 이용한 단백질 분리 시스템
JP7399845B2 (ja) * 2017-08-18 2023-12-18 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 試料マトリックスにおいてタンパク質変異体を分析するための画像キャピラリー等電点電気泳動法
JP7172674B2 (ja) * 2019-02-04 2022-11-16 株式会社島津製作所 フィールドフローフラクショネーション装置及び洗浄方法
US11971392B2 (en) * 2019-06-25 2024-04-30 Wyatt Technology, Llc Sealing structure for a field flow fractionator
EP4169474A1 (en) 2021-10-25 2023-04-26 Erbe Vision GmbH System and method for image registration

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100792683B1 (ko) * 2006-05-09 2008-01-09 연세대학교 산학협력단 모세관등전집중-중공사흐름장흐름분획법을 이용한 단백질분리장치 및 분리방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0727837B2 (ja) * 1988-06-24 1995-03-29 株式会社村田製作所 圧電部品の製造方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100792683B1 (ko) * 2006-05-09 2008-01-09 연세대학교 산학협력단 모세관등전집중-중공사흐름장흐름분획법을 이용한 단백질분리장치 및 분리방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KANG DUKJIN ET AL.: "Development of Non-Gel-Based Two-Dimensional Separation of Intact Proteins by an On-Line Hyphenation of Capillary Isoelectric Focusing and Hollow Fiber Flow Field-Flow Fractionation", ANAL. CHEM., 2006, pages 5789 - 5798 *
OH SUNOK ET AL.: "Miniaturized asymmetrical flow field-flow fractionation:Application to biological vesicles", J. SEP. SCI., 2007, pages 1082 - 1087 *
OH SUNOK: "A study on specific character of subcellular species using asymmetrical flow filed-flow fractionation", MASTER'S THESIS OF GRADUATE SCHOOL OF YONSEI UNIVERSITY, 11 April 2007 (2007-04-11) *

Also Published As

Publication number Publication date
US8298394B2 (en) 2012-10-30
WO2009145532A2 (ko) 2009-12-03
KR20090122836A (ko) 2009-12-01
US20110253535A1 (en) 2011-10-20
KR100958165B1 (ko) 2010-05-14

Similar Documents

Publication Publication Date Title
WO2009145532A3 (ko) 비젤방식의 2차원 단백질분리용 다중채널장치
DE602007011937D1 (de) Peptidantikörperdepletion und anwendung zur probenvorbereitung für massenspektrometrie
DK2126586T3 (da) Direkte bestemmelse af vitamin D i serum eller plasma
EP4249605A3 (en) Methods for analyte detection
WO2007041692A3 (en) Microfluidic detection of analytes
ATE510212T1 (de) Reagenz zur analyse unreifer leukozyten und reagenzkit
MX2009013388A (es) Analisis multiple de muestras de sangre.
WO2012148497A3 (en) Identification of polynucleotides associated with a sample
AU2003224674A1 (en) Compounds and methods for analyzing the proteome
WO2009121032A3 (en) Sample preparation devices and methods for processing analytes
WO2007115807A8 (en) Apparatus for performing dissolved gas analysis
WO2007017085A3 (en) Process for cross-linking cellulose ester membranes
EP2949667A3 (en) Recombinant cell surface capture proteins
WO2009121034A3 (en) Multicapillary sample preparation devices and methods for processing analytes
MX2021012677A (es) Identificacion de proteinas de celulas huesped.
WO2008151207A3 (en) Expression quantification using mass spectrometry
AU2017266331A8 (en) Blood sample analysis method and system, for determining diabetes
Krisp et al. Online Peptide Fractionation Using a Multiphasic Microfluidic Liquid Chromatography Chip Improves Reproducibility and Detection Limits for Quantitation in Discovery and Targeted Proteomics*[S]
DE602007008872D1 (de) Mikrowellengestützte deglycosylierung von proteinen zur molekulargewichtsbestimmung durch massenspektrometrie
EP2176432A4 (en) METHOD OF DETECTING DISEASE MARKERS
WO2008008862A3 (en) Proteome standards for mass spectrometry
UA112515C2 (uk) Спосіб виявлення присутності двох або більше білків, що становлять інтерес, в зразках рослинного походження
WO2021202542A3 (en) Methods of profiling translation rate
WO2023141548A3 (en) Methods for detecting proteins and for co-detecting proteins and nucleic acids, and kits for the same
WO2011001274A3 (en) Methods for distinguishing between natural and artificial dna samples

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 12531281

Country of ref document: US

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09754997

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09754997

Country of ref document: EP

Kind code of ref document: A2