MX2021004651A - Adaptacion y optimizacion del proceso de microorganismos para el crecimiento en carbohidratos derivados de hemicelulosicos. - Google Patents

Adaptacion y optimizacion del proceso de microorganismos para el crecimiento en carbohidratos derivados de hemicelulosicos.

Info

Publication number
MX2021004651A
MX2021004651A MX2021004651A MX2021004651A MX2021004651A MX 2021004651 A MX2021004651 A MX 2021004651A MX 2021004651 A MX2021004651 A MX 2021004651A MX 2021004651 A MX2021004651 A MX 2021004651A MX 2021004651 A MX2021004651 A MX 2021004651A
Authority
MX
Mexico
Prior art keywords
microorganisms
xylose
methods
population
hemicellulosic
Prior art date
Application number
MX2021004651A
Other languages
English (en)
Inventor
Alexandra Merkx-Jacques
David Woodhall
Denise Muise
Zachary Sun
Roberto E Armenta
Kimberly Hyson
Nathalia Florez
Dorothy Dennis
Original Assignee
Mara Renewables Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mara Renewables Corp filed Critical Mara Renewables Corp
Publication of MX2021004651A publication Critical patent/MX2021004651A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente se proporcionan métodos para preparar microorganismos modificados para un mayor consumo de xilosa en comparación con microorganismos no modificados. Los métodos incluyen proporcionar microorganismos consumidores de xilosa que comprenden dos o más copias de una secuencia de ácido nucleico que codifica xilosa isomerasa y dos o más copias de una secuencia de ácido nucleico que codifica una xilosa quinasa, cultivar los microorganismos en un medio que contiene xilosa y recolectar una porción de los microorganismos. Estas etapas se repiten varias veces. A continuación, se aíslan los microorganismos. Los microorganismos aislados tienen mayores proporciones de consumo de xilosa en comparación con los microorganismos de control que consumen xilosa. También se proporciona una población de microorganismos elaborada mediante los métodos proporcionados. Se proporcionan métodos para cultivar la población de microorganismos y métodos para reducir la producción de xilitol en cultivos que comprenden la población de microorganismos.
MX2021004651A 2018-10-23 2019-10-21 Adaptacion y optimizacion del proceso de microorganismos para el crecimiento en carbohidratos derivados de hemicelulosicos. MX2021004651A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862749554P 2018-10-23 2018-10-23
PCT/IB2019/058953 WO2020084445A1 (en) 2018-10-23 2019-10-21 Adaptation and process optimization of microorganisms for growth in hemicellulosic derived carbohydrates

Publications (1)

Publication Number Publication Date
MX2021004651A true MX2021004651A (es) 2021-05-28

Family

ID=70331356

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021004651A MX2021004651A (es) 2018-10-23 2019-10-21 Adaptacion y optimizacion del proceso de microorganismos para el crecimiento en carbohidratos derivados de hemicelulosicos.

Country Status (8)

Country Link
US (1) US20210388312A1 (es)
EP (1) EP3870692A4 (es)
CN (1) CN112912489A (es)
AU (1) AU2019365525A1 (es)
BR (1) BR112021007723A2 (es)
CA (1) CA3116984A1 (es)
MX (1) MX2021004651A (es)
WO (1) WO2020084445A1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009434A1 (en) * 2004-07-16 2006-01-26 Technische Universiteit Delft Metabolic engineering of xylose fermenting eukaryotic cells
AU2009228323B2 (en) * 2008-03-27 2014-01-30 Alliance For Sustainable Energy, Llc Zymomonas with improved xylose utilization
EP2519642B1 (en) * 2009-12-28 2017-10-25 DSM IP Assets B.V. Recombinant thraustochytrids that grow on xylose, and compositions, methods of making, and uses thereof
CN103608450A (zh) * 2011-05-06 2014-02-26 索拉兹米公司 基因工程改造的代谢木糖的微生物
KR101654219B1 (ko) * 2015-04-21 2016-09-05 한국생명공학연구원 고 함량의 다중불포화지방산을 함유하는 트라우스토키트리대 속 균주 및 이의 용도
KR20180027584A (ko) * 2015-07-13 2018-03-14 마라 리뉴어블즈 코퍼레이션 크실로오스의 미세조류 대사를 강화시키는 방법

Also Published As

Publication number Publication date
WO2020084445A1 (en) 2020-04-30
EP3870692A1 (en) 2021-09-01
BR112021007723A2 (pt) 2021-08-10
AU2019365525A1 (en) 2021-05-13
CN112912489A (zh) 2021-06-04
CA3116984A1 (en) 2020-04-30
US20210388312A1 (en) 2021-12-16
EP3870692A4 (en) 2022-10-19

Similar Documents

Publication Publication Date Title
ES2856749T3 (es) Proceso para la producción de oligosacáridos fucosilados
CA3005308C (en) Media and fermentation methods for producing polysaccharides in bacterial cell culture
ZA200605440B (en) Advanced microorganism for producing 1,2-propanediol
NO20053357L (no) Dyrkingsmedium, dyrkingsmetode og de oppnadde myoblaster og deres anvendelse.
MX343903B (es) Procedimiento de fermentación.
UA108468C2 (en) Microorganism that expresses xylose isomerase
WO2009073557A3 (en) Biological systems for production of commercially valuable compounds
MX2022010521A (es) Metodo para producir linfocitos citoliticos naturales a partir de celulas madre pluripotentes.
MX2018012297A (es) Metodo para la produccion de acido lactico.
MY170513A (en) Method of producing succinic acid and other chemicals using sucrose-containing feedstock
WO2020106870A3 (en) Biosynthesis of compounds in yeast
MY185050A (en) A pentose sugar fermenting cell
WO2014059275A3 (en) Process for producing gougerotin employing streptomyces microflavus strains
MX2017011220A (es) Metodos para hacer celulas madre neurales y usos de las mismas.
MY195280A (en) Nucleic Acid Systems That Enable Bacteria to Specifically Target Solid Tumors Via Glucose-Dependent Viability
MY188848A (en) Yeast propagation simultanesous with saccharification
MX2022001814A (es) Produccion mejorada de oligosacaridos en levadura.
CN104087535B (zh) 生物法制备茶氨酸的方法
BR112016017881A2 (pt) processo para obtenção de uma cepa de levedura, levedura, cepa de levedura, método para produzir pelo menos um produto de fermentação, e, uso de uma levedura
MX2021004651A (es) Adaptacion y optimizacion del proceso de microorganismos para el crecimiento en carbohidratos derivados de hemicelulosicos.
AU2016288656A8 (en) Process for producing lactic acid or its salts from fermentation using thermotolerance bacillus bacteria
WO2019013493A3 (ko) 고농도 세포 배양 방법을 이용한 다당체의 생산 방법
RU2013146377A (ru) СПОСОБ ПОЛУЧЕНИЯ ИЗОПРЕНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ РОДА Bacillus
CN102746997B (zh) 一种扁座壳孢菌菌丝快速生长培养基
KR101887821B1 (ko) 비산화 펜토오스 인산 경로 관련 효소의 불활성화에 의한 히스티딘 생산능 변이 균주