BR112016017881A2 - processo para obtenção de uma cepa de levedura, levedura, cepa de levedura, método para produzir pelo menos um produto de fermentação, e, uso de uma levedura - Google Patents

processo para obtenção de uma cepa de levedura, levedura, cepa de levedura, método para produzir pelo menos um produto de fermentação, e, uso de uma levedura

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Publication number
BR112016017881A2
BR112016017881A2 BR112016017881A BR112016017881A BR112016017881A2 BR 112016017881 A2 BR112016017881 A2 BR 112016017881A2 BR 112016017881 A BR112016017881 A BR 112016017881A BR 112016017881 A BR112016017881 A BR 112016017881A BR 112016017881 A2 BR112016017881 A2 BR 112016017881A2
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BR
Brazil
Prior art keywords
strain
yeast
yeast strain
recombinant yeast
obtaining
Prior art date
Application number
BR112016017881A
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English (en)
Other versions
BR112016017881B1 (pt
Inventor
Pignede Georges
Techel Jennifer
Desfougeres Thomas
Original Assignee
Lesaffre & Cie
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Publication date
Application filed by Lesaffre & Cie filed Critical Lesaffre & Cie
Publication of BR112016017881A2 publication Critical patent/BR112016017881A2/pt
Publication of BR112016017881B1 publication Critical patent/BR112016017881B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01006—Galactokinase (2.7.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se a um método para a obtenção de uma cepa capaz de propagar eficientemente em um meio de baixo potencial nutritivo, capaz de metabolizar pentoses e de resistir aos inibidores de fermentação, compreendendo as seguintes etapas; a) cultivo de uma cepa de levedura recombinante com uma cepa de levedura selvagem desprovida de deficiências, a cepa de levedura recombinante compreendendo pelo menos uma cópia de um gene exógeno da xilose isomerase e pelo menos uma cópia supernumerária supernumerosa de um gene de d-xilulocinase incluída no genoma e ligado a uma única característica sexual da cepa, b) pelo menos dois ciclos de mistura genética pela esporulação e ou hibridização aleatória, c) seleção da população obtida na etapa b) de acordo com um critério de adequabilidade das cepas para metabolizar xilose, d) seleção da população obtida na etapa c) de acordo com um critério de adequabilidade das cepas para crescer em um meio tipo pref, um meio com valor nutritivo baixo. a invenção também se refere a uma célula, uma levedura ou uma cepa de levedura recombi¬nante obtida de acordo com o método, e ao uso de uma tal célula para a produção de bioetanol.
BR112016017881-5A 2014-02-17 2015-02-16 Processo para obtenção de uma cepa de levedura, levedura, cepa de levedura, método para produzir pelo menos um produto de fermentação, e, uso de uma levedura BR112016017881B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1451246 2014-02-17
FR1451246A FR3017623B1 (fr) 2014-02-17 2014-02-17 Souche fermentant les pentoses a propagation optimisee
PCT/FR2015/050364 WO2015121595A1 (fr) 2014-02-17 2015-02-16 Souche fermentant les pentoses a propagation optimisée

Publications (2)

Publication Number Publication Date
BR112016017881A2 true BR112016017881A2 (pt) 2017-10-17
BR112016017881B1 BR112016017881B1 (pt) 2023-04-11

Family

ID=51063547

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016017881-5A BR112016017881B1 (pt) 2014-02-17 2015-02-16 Processo para obtenção de uma cepa de levedura, levedura, cepa de levedura, método para produzir pelo menos um produto de fermentação, e, uso de uma levedura

Country Status (16)

Country Link
US (1) US10273447B2 (pt)
EP (1) EP3108016B1 (pt)
JP (1) JP6653659B2 (pt)
CN (1) CN106414735B (pt)
AU (1) AU2015216821B2 (pt)
BR (1) BR112016017881B1 (pt)
CA (1) CA2938962C (pt)
DK (1) DK3108016T3 (pt)
ES (1) ES2695728T3 (pt)
FR (1) FR3017623B1 (pt)
HR (1) HRP20181793T1 (pt)
HU (1) HUE040429T2 (pt)
MY (1) MY191221A (pt)
PL (1) PL3108016T3 (pt)
PT (1) PT3108016T (pt)
WO (1) WO2015121595A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3062134B1 (fr) 2017-01-24 2023-07-21 Lesaffre & Cie Obtention de souches de levure performantes pour la metabolisation de l'arabinose
KR20200049814A (ko) 2017-09-01 2020-05-08 에스더블유아이엠씨 엘엘씨 멀티-스테이지 중합체 라텍스, 그러한 라텍스를 함유하는 코팅 조성물, 및 이로 코팅된 물품
CN111278933A (zh) 2017-09-01 2020-06-12 宣伟投资管理有限公司 多级聚合物胶乳、含有该胶乳的涂料及其涂布的制品
CN110734937A (zh) * 2018-07-20 2020-01-31 远东新世纪股份有限公司 用于生产乳酸的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005121337A1 (en) * 2004-06-08 2005-12-22 Microbiogen Pty. Ltd. Non-recombinant saccharomyces strains that grow on xylose
DE102008031350B4 (de) 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
US8440449B2 (en) * 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
WO2011079388A1 (en) * 2009-12-30 2011-07-07 Iogen Energy Corporation Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates
FR2968313B1 (fr) * 2010-12-03 2014-10-10 Lesaffre & Cie Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose
US20140322776A1 (en) * 2011-11-29 2014-10-30 Codexis, Inc. Overexpression of genes that improve fermentation in yeast using cellulosic substrates
FR2991339B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Souches de levure aptes a metaboliser le xylose et resistantes a au moins un inhibiteur de fermentation, procede d'obtention et utilisation
FR2991340B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Procede d'obtention de souches de levure ameliorees par modification genetique et croisement

Also Published As

Publication number Publication date
DK3108016T3 (en) 2018-11-26
EP3108016A1 (fr) 2016-12-28
HRP20181793T1 (hr) 2018-12-28
CA2938962C (en) 2022-07-19
CN106414735A (zh) 2017-02-15
ES2695728T3 (es) 2019-01-10
AU2015216821A1 (en) 2016-08-04
WO2015121595A1 (fr) 2015-08-20
FR3017623A1 (fr) 2015-08-21
US10273447B2 (en) 2019-04-30
MY191221A (en) 2022-06-09
CA2938962A1 (en) 2015-08-20
EP3108016B1 (fr) 2018-10-24
FR3017623B1 (fr) 2018-08-10
BR112016017881B1 (pt) 2023-04-11
US20160348064A1 (en) 2016-12-01
JP2017505627A (ja) 2017-02-23
HUE040429T2 (hu) 2019-03-28
PT3108016T (pt) 2018-11-21
JP6653659B2 (ja) 2020-02-26
AU2015216821B2 (en) 2021-07-08
PL3108016T3 (pl) 2019-01-31
CN106414735B (zh) 2021-03-16

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