MX2021003746A - Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas. - Google Patents

Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas.

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Publication number
MX2021003746A
MX2021003746A MX2021003746A MX2021003746A MX2021003746A MX 2021003746 A MX2021003746 A MX 2021003746A MX 2021003746 A MX2021003746 A MX 2021003746A MX 2021003746 A MX2021003746 A MX 2021003746A MX 2021003746 A MX2021003746 A MX 2021003746A
Authority
MX
Mexico
Prior art keywords
methods
nuclei
making
throughput
cell libraries
Prior art date
Application number
MX2021003746A
Other languages
English (en)
Inventor
Sanjay Srivatsan
Jay Shendure
Jose Mcfaline-Figueroa
Vijay Ramani
Junyue Cao
Gregory Booth
Cole Trapnell
Frank J Steemers
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021003746A publication Critical patent/MX2021003746A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)

Abstract

En la presente descripción se proporcionan métodos para preparar una genoteca de secuenciación que incluye ácidos nucleicos de una pluralidad de células únicas. En una modalidad, el método incluye el hashing nuclear o celular que permite el rendimiento aumentado de muestra y la detección aumentada de doblete a altos índices de colisión. En una modalidad, el método incluye hash de normalización que ayuda a estimar y eliminar el ruido técnico en la variación de célula a célula y aumenta la sensibilidad y especificidad.
MX2021003746A 2019-03-01 2020-03-02 Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas. MX2021003746A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962812853P 2019-03-01 2019-03-01
PCT/US2020/020637 WO2020180778A1 (en) 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
MX2021003746A true MX2021003746A (es) 2021-06-23

Family

ID=70110324

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021003746A MX2021003746A (es) 2019-03-01 2020-03-02 Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas.

Country Status (12)

Country Link
US (1) US20220033805A1 (es)
EP (1) EP3837365A1 (es)
JP (2) JP2022522917A (es)
KR (1) KR20210134598A (es)
CN (1) CN113302300A (es)
AU (1) AU2020232618A1 (es)
BR (1) BR112021006234A2 (es)
CA (1) CA3113841A1 (es)
IL (1) IL281829A (es)
MX (1) MX2021003746A (es)
SG (1) SG11202102530QA (es)
WO (1) WO2020180778A1 (es)

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CN114107459B (zh) * 2021-11-11 2022-12-06 浙江大学 一种基于寡核苷酸链杂交标记的高通量单细胞测序方法
CN116694730A (zh) * 2022-02-28 2023-09-05 南方科技大学 一种单细胞开放染色质和转录组共测序文库的构建方法
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Also Published As

Publication number Publication date
BR112021006234A2 (pt) 2021-09-28
KR20210134598A (ko) 2021-11-10
CA3113841A1 (en) 2020-09-10
EP3837365A1 (en) 2021-06-23
WO2020180778A1 (en) 2020-09-10
WO2020180778A9 (en) 2021-04-08
JP2023062105A (ja) 2023-05-02
CN113302300A (zh) 2021-08-24
SG11202102530QA (en) 2021-04-29
AU2020232618A1 (en) 2021-04-08
US20220033805A1 (en) 2022-02-03
JP2022522917A (ja) 2022-04-21
IL281829A (en) 2021-05-31

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