MX2020014066A - Celdas de flujo y kits de secuenciacion. - Google Patents
Celdas de flujo y kits de secuenciacion.Info
- Publication number
- MX2020014066A MX2020014066A MX2020014066A MX2020014066A MX2020014066A MX 2020014066 A MX2020014066 A MX 2020014066A MX 2020014066 A MX2020014066 A MX 2020014066A MX 2020014066 A MX2020014066 A MX 2020014066A MX 2020014066 A MX2020014066 A MX 2020014066A
- Authority
- MX
- Mexico
- Prior art keywords
- depressions
- substrate
- electrode
- functionalized surface
- flow cells
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00608—DNA chips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/00626—Covalent
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/103—Nucleic acid detection characterized by the use of physical, structural and functional properties luminescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
Abstract
En un ejemplo, una celda de flujo incluye un sustrato, un electrodo colocado sobre el sustrato y un material modelado colocado sobre el electrodo. En este ejemplo, el material modelado incluye depresiones separadas por regiones intersticiales, y una superficie funcionalizada del electrodo está expuesta en cada una de las depresiones. En este ejemplo, la celda de flujo incluye además un cebador injertado a la superficie funcionalizada en cada una de las depresiones. En otro ejemplo, una celda de flujo incluye un sustrato y un electrodo modelado colocado sobre el sustrato. En este otro ejemplo, el electrodo modelado incluye depresiones separadas por regiones intersticiales y una superficie funcionalizada del sustrato expuesta en cada una de las depresiones. En este otro ejemplo, se injerta un cebador a la superficie funcionalizada en cada una de las depresiones.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862780602P | 2018-12-17 | 2018-12-17 | |
PCT/US2019/064197 WO2020131354A1 (en) | 2018-12-17 | 2019-12-03 | Flow cells and sequencing kits |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2020014066A true MX2020014066A (es) | 2021-05-27 |
Family
ID=71073550
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2020014066A MX2020014066A (es) | 2018-12-17 | 2019-12-03 | Celdas de flujo y kits de secuenciacion. |
Country Status (13)
Country | Link |
---|---|
US (1) | US11841310B2 (es) |
EP (1) | EP3899543A4 (es) |
JP (1) | JP2022513547A (es) |
KR (1) | KR20210093749A (es) |
CN (2) | CN212451364U (es) |
AU (1) | AU2019406313A1 (es) |
BR (1) | BR112020026320A2 (es) |
CA (1) | CA3103525A1 (es) |
IL (1) | IL279670A (es) |
MX (1) | MX2020014066A (es) |
SG (1) | SG11202012634UA (es) |
TW (1) | TWI760667B (es) |
WO (1) | WO2020131354A1 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6899588B2 (ja) * | 2018-11-20 | 2021-07-07 | 国立研究開発法人産業技術総合研究所 | 液体操作装置 |
JP2023544468A (ja) * | 2020-09-29 | 2023-10-24 | イルミナ インコーポレイテッド | フローセルを作製するための方法 |
MX2022014809A (es) * | 2020-09-29 | 2023-03-14 | Illumina Inc | Celdas de flujo y metodos para hacer las mismas. |
CN115917004A (zh) * | 2020-11-16 | 2023-04-04 | 伊鲁米纳剑桥有限公司 | 改变流通池信号 |
US20220162689A1 (en) * | 2020-11-23 | 2022-05-26 | Qiagen Sciences, Llc | Method and device for sequencing and detecting dna/rna directly on a sensor |
KR20230128487A (ko) * | 2020-12-15 | 2023-09-05 | 일루미나, 인코포레이티드 | 플로우 셀 코팅 방법 |
CN113686760B (zh) * | 2021-02-03 | 2022-10-28 | 帝迈(苏州)生物技术有限公司 | 试剂盒、poct血细胞分析仪 |
EP4299562A1 (en) * | 2021-02-26 | 2024-01-03 | Elips Diagnostics Inc. | Novel electrochemiluminescence co-reactant, and electrochemiluminescence system comprising same |
US20230183799A1 (en) * | 2021-12-10 | 2023-06-15 | Illumina, Inc. | Parallel sample and index sequencing |
CA3223729A1 (en) * | 2022-03-01 | 2023-09-07 | Gianluca Andrea ARTIOLI | Functionalized nanostructures and flow cell depressions |
WO2023175041A1 (en) * | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides |
EP4286042A1 (en) * | 2022-05-31 | 2023-12-06 | Imec VZW | A molecular synthesis array |
EP4286041A1 (en) * | 2022-05-31 | 2023-12-06 | Imec VZW | A molecular synthesis array |
WO2023240090A1 (en) * | 2022-06-07 | 2023-12-14 | Illumina, Inc. | Enhancing clustering efficiency and kinetics |
WO2024073599A1 (en) * | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6569382B1 (en) * | 1991-11-07 | 2003-05-27 | Nanogen, Inc. | Methods apparatus for the electronic, homogeneous assembly and fabrication of devices |
US6652808B1 (en) * | 1991-11-07 | 2003-11-25 | Nanotronics, Inc. | Methods for the electronic assembly and fabrication of devices |
US5786141A (en) * | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
US6316180B1 (en) | 1995-01-04 | 2001-11-13 | Igen International, Inc. | Electrochemiluminescent monitoring of compounds/electrochemiluminescence assays |
CN1192097C (zh) | 1995-03-10 | 2005-03-09 | 梅索磅秤技术有限公司 | 多阵列、多特异性的电化学发光检验 |
EP2420824B1 (en) | 2001-06-29 | 2018-11-28 | Meso Scale Technologies LLC | Multi-well plate having an array of wells and kit for use in the conduct of an ECL assay |
DE10133844B4 (de) | 2001-07-18 | 2006-08-17 | Micronas Gmbh | Verfahren und Vorrichtung zur Detektion von Analyten |
DE50203024D1 (de) | 2001-08-24 | 2005-06-09 | Georg Issakides | Bindegarn für glühkörper und dessen verwendung |
EP1436598B1 (en) | 2001-09-10 | 2013-11-27 | Meso Scale Technologies LLC | Assay buffer, compositions containing the same, and methods of using the same |
US20050019803A1 (en) * | 2003-06-13 | 2005-01-27 | Liu Timothy Z. | Array electrode |
US20060257560A1 (en) * | 2004-12-30 | 2006-11-16 | Affymetrix, Inc. | Polymer surfaces for insitu synthesis of polymer arrays |
CN100549921C (zh) * | 2006-11-28 | 2009-10-14 | 致伸科技股份有限公司 | 具有倾斜式滚轮的编码装置 |
DE102011015184B4 (de) | 2010-06-02 | 2013-11-21 | Thinxxs Microtechnology Ag | Vorrichtung für den Transport kleiner Volumina eines Fluids, insbesondere Mikropumpe oder Mikroventil |
EP2954315A2 (en) * | 2013-02-07 | 2015-12-16 | Koninklijke Philips N.V. | Processing of nucleotide sequences |
KR102177225B1 (ko) | 2013-07-01 | 2020-11-10 | 일루미나, 인코포레이티드 | 촉매-무함유 표면 작용화 및 중합체 그라프팅 |
ES2848062T3 (es) | 2013-12-23 | 2021-08-05 | Illumina Inc | Sustratos estructurados para mejorar la detección de emisiones de luz y métodos relacionados con los mismos |
US10537889B2 (en) * | 2013-12-31 | 2020-01-21 | Illumina, Inc. | Addressable flow cell using patterned electrodes |
SG11201811064TA (en) * | 2016-06-20 | 2019-01-30 | F Star Delta Ltd | Binding molecules binding pd-l1 and lag-3 |
WO2018119101A1 (en) * | 2016-12-22 | 2018-06-28 | Illumina, Inc. | Flow cell package and method for making the same |
WO2020037286A1 (en) * | 2018-08-17 | 2020-02-20 | Life Technologies Corporation | System and method for preparation of nucleotide solutions |
-
2019
- 2019-12-03 KR KR1020207037480A patent/KR20210093749A/ko unknown
- 2019-12-03 SG SG11202012634UA patent/SG11202012634UA/en unknown
- 2019-12-03 AU AU2019406313A patent/AU2019406313A1/en active Pending
- 2019-12-03 WO PCT/US2019/064197 patent/WO2020131354A1/en unknown
- 2019-12-03 EP EP19899114.3A patent/EP3899543A4/en active Pending
- 2019-12-03 MX MX2020014066A patent/MX2020014066A/es unknown
- 2019-12-03 CA CA3103525A patent/CA3103525A1/en active Pending
- 2019-12-03 BR BR112020026320-6A patent/BR112020026320A2/pt unknown
- 2019-12-03 JP JP2020572658A patent/JP2022513547A/ja active Pending
- 2019-12-03 US US16/701,863 patent/US11841310B2/en active Active
- 2019-12-12 TW TW108145505A patent/TWI760667B/zh active
- 2019-12-17 CN CN201922270259.9U patent/CN212451364U/zh active Active
- 2019-12-17 CN CN201911305111.2A patent/CN111321054A/zh active Pending
-
2020
- 2020-12-22 IL IL279670A patent/IL279670A/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2020131354A9 (en) | 2020-09-10 |
EP3899543A1 (en) | 2021-10-27 |
IL279670A (en) | 2021-03-01 |
CA3103525A1 (en) | 2020-06-25 |
WO2020131354A1 (en) | 2020-06-25 |
JP2022513547A (ja) | 2022-02-09 |
US20200191699A1 (en) | 2020-06-18 |
BR112020026320A2 (pt) | 2021-03-30 |
TWI760667B (zh) | 2022-04-11 |
US11841310B2 (en) | 2023-12-12 |
AU2019406313A1 (en) | 2021-01-07 |
EP3899543A4 (en) | 2022-08-17 |
TW202035970A (zh) | 2020-10-01 |
KR20210093749A (ko) | 2021-07-28 |
SG11202012634UA (en) | 2021-01-28 |
CN111321054A (zh) | 2020-06-23 |
CN212451364U (zh) | 2021-02-02 |
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