MX2022014810A - Alteración de señales de celdas de flujo. - Google Patents

Alteración de señales de celdas de flujo.

Info

Publication number
MX2022014810A
MX2022014810A MX2022014810A MX2022014810A MX2022014810A MX 2022014810 A MX2022014810 A MX 2022014810A MX 2022014810 A MX2022014810 A MX 2022014810A MX 2022014810 A MX2022014810 A MX 2022014810A MX 2022014810 A MX2022014810 A MX 2022014810A
Authority
MX
Mexico
Prior art keywords
flow cell
cell signals
altering flow
hydrogel
grafted
Prior art date
Application number
MX2022014810A
Other languages
English (en)
Inventor
Gianluca Andrea Artioli
Hatten Xavier Von
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of MX2022014810A publication Critical patent/MX2022014810A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N15/1436—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Control Of Eletrric Generators (AREA)
  • Steering Control In Accordance With Driving Conditions (AREA)
  • Vehicle Body Suspensions (AREA)

Abstract

En un método ilustrativo, se aplica un hidrogel a una superficie de un sustrato y se injertan cebadores en el hidrogel aplicado. Antes o después de injertar los cebadores, se introducen nanoestructuras plasmónicas en el hidrogel aplicado. Este sustrato puede formar una superficie de una celda de flujo. Cuando la celda de flujo se utiliza en una operación de secuenciación, las nanoestructuras plasmónicas pueden mejorar las señales fluorescentes que se generan.
MX2022014810A 2020-11-16 2021-11-12 Alteración de señales de celdas de flujo. MX2022014810A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063114305P 2020-11-16 2020-11-16
PCT/EP2021/081502 WO2022101401A1 (en) 2020-11-16 2021-11-12 Altering flow cell signals

Publications (1)

Publication Number Publication Date
MX2022014810A true MX2022014810A (es) 2023-03-06

Family

ID=78806469

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022014810A MX2022014810A (es) 2020-11-16 2021-11-12 Alteración de señales de celdas de flujo.

Country Status (10)

Country Link
US (1) US20220155211A1 (es)
EP (1) EP4244387A1 (es)
JP (1) JP2023550676A (es)
KR (1) KR20230108223A (es)
CN (1) CN115917004A (es)
AU (1) AU2021380016A1 (es)
CA (1) CA3183193A1 (es)
MX (1) MX2022014810A (es)
TW (1) TW202235627A (es)
WO (1) WO2022101401A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240002933A1 (en) * 2022-06-30 2024-01-04 Illumina Cambridge Limited Sequencing nanoparticles and methods of making the same
US20240102913A1 (en) * 2022-08-26 2024-03-28 Illumina, Inc. Flow cells with patterned bonding regions

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102333635B1 (ko) * 2013-12-23 2021-11-30 일루미나, 인코포레이티드 광 방출의 검출을 개선시키기 위한 구조화 기판 및 이와 관련한 방법
EP3970849A1 (en) * 2014-10-31 2022-03-23 Illumina Cambridge Limited Polymers and dna copolymer coatings
WO2016168386A1 (en) * 2015-04-14 2016-10-20 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
CN107835857A (zh) * 2015-07-17 2018-03-23 亿明达股份有限公司 用于测序应用的聚合物片层
NL2019044B1 (en) * 2017-05-11 2018-11-15 Illumina Inc Protective surface coatings for flow cells
US11352668B2 (en) * 2017-08-01 2022-06-07 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
AU2018392919B2 (en) * 2017-12-21 2021-04-15 Illumina Cambridge Limited Flow cells with hydrogel coating
US11841310B2 (en) * 2018-12-17 2023-12-12 Illumina, Inc. Flow cells and sequencing kits

Also Published As

Publication number Publication date
CA3183193A1 (en) 2022-05-19
CN115917004A (zh) 2023-04-04
EP4244387A1 (en) 2023-09-20
WO2022101401A1 (en) 2022-05-19
KR20230108223A (ko) 2023-07-18
JP2023550676A (ja) 2023-12-05
AU2021380016A1 (en) 2023-01-05
TW202235627A (zh) 2022-09-16
US20220155211A1 (en) 2022-05-19

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