MX2021010496A - Kits y celdas de flujo. - Google Patents

Kits y celdas de flujo.

Info

Publication number
MX2021010496A
MX2021010496A MX2021010496A MX2021010496A MX2021010496A MX 2021010496 A MX2021010496 A MX 2021010496A MX 2021010496 A MX2021010496 A MX 2021010496A MX 2021010496 A MX2021010496 A MX 2021010496A MX 2021010496 A MX2021010496 A MX 2021010496A
Authority
MX
Mexico
Prior art keywords
cleavage
flow cell
polymeric hydrogel
kits
catalytic polymeric
Prior art date
Application number
MX2021010496A
Other languages
English (en)
Inventor
Brian D Mather
Andrew A Brown
Wayne N George
Xiaohai Liu
Antoine Francais
Bacigalupo Maria Candelaria Rogert
Pietro Gatti Lafranconi
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021010496A publication Critical patent/MX2021010496A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Un ejemplo de un kit incluye una celda de flujo y una mezcla de escisión. Un ejemplo de celda de flujo incluye un sustrato; un hidrogel polimérico catalítico en el sustrato, el hidrogel polimérico catalítico incluye un catalizador de desbloqueo; y un cebador de amplificación unido al hidrogel polimérico catalítico. El catalizador de desbloqueo acelera la escisión de un grupo de bloqueo de un nucleótido bloqueado con OH en 3' introducido en la celda de flujo e incorporado en una cadena de plantilla unida al cebador de amplificación. Un ejemplo de la mezcla de escisión incluye un componente para iniciar la escisión del grupo de bloqueo.
MX2021010496A 2019-12-16 2020-11-25 Kits y celdas de flujo. MX2021010496A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962948605P 2019-12-16 2019-12-16
PCT/US2020/062279 WO2021126503A1 (en) 2019-12-16 2020-11-25 Kits and flow cells

Publications (1)

Publication Number Publication Date
MX2021010496A true MX2021010496A (es) 2022-01-18

Family

ID=73839126

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021010496A MX2021010496A (es) 2019-12-16 2020-11-25 Kits y celdas de flujo.

Country Status (11)

Country Link
US (1) US20220145379A1 (es)
EP (1) EP4077726A1 (es)
JP (1) JP2023506103A (es)
KR (1) KR20220115857A (es)
CN (1) CN113853442A (es)
AU (1) AU2020407967A1 (es)
BR (1) BR112021019641A2 (es)
CA (1) CA3135280A1 (es)
IL (1) IL286668A (es)
MX (1) MX2021010496A (es)
WO (1) WO2021126503A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019028058A2 (pt) 2018-06-29 2020-07-14 Illumina, Inc. células de fluxo
EP4363615A1 (en) * 2021-07-02 2024-05-08 Illumina, Inc. Flow cells
US20230101095A1 (en) * 2021-09-29 2023-03-30 Illumina Cambridge Limited Flow cells with dark quencher
US20240132956A1 (en) * 2022-09-29 2024-04-25 Illumina, Inc. Nucleic acid sequencing components including a glycolipid bi-layer

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110059865A1 (en) * 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
US20060210996A1 (en) * 2005-03-15 2006-09-21 Eric Leproust Methods for in situ generation of chemical arrays
US8715932B2 (en) * 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
US9540688B2 (en) * 2014-02-19 2017-01-10 Personal Genomics, Inc. Real-time sequencing method for single molecule
US20170240961A1 (en) * 2016-02-23 2017-08-24 Complete Genomics, Inc. Affinity tag labeled nucleosides and uses
CN109790196B (zh) * 2016-04-22 2022-09-27 深圳华大智造科技股份有限公司 可逆封闭的核苷类似物及其用途
WO2018129214A1 (en) * 2017-01-04 2018-07-12 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
WO2019126040A1 (en) * 2017-12-21 2019-06-27 Illumina, Inc. Flow cells with hydrogel coating
JP7143341B2 (ja) * 2018-03-09 2022-09-28 イルミナ ケンブリッジ リミテッド 一般化確率的超解像シーケンシング

Also Published As

Publication number Publication date
WO2021126503A1 (en) 2021-06-24
EP4077726A1 (en) 2022-10-26
BR112021019641A2 (pt) 2022-06-21
IL286668A (en) 2021-12-01
US20220145379A1 (en) 2022-05-12
AU2020407967A1 (en) 2021-09-23
CA3135280A1 (en) 2021-06-24
CN113853442A (zh) 2021-12-28
KR20220115857A (ko) 2022-08-19
JP2023506103A (ja) 2023-02-15

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