MX2019007444A - Metodos y sistemas para analisis de moleculas de acido nucleico. - Google Patents

Metodos y sistemas para analisis de moleculas de acido nucleico.

Info

Publication number
MX2019007444A
MX2019007444A MX2019007444A MX2019007444A MX2019007444A MX 2019007444 A MX2019007444 A MX 2019007444A MX 2019007444 A MX2019007444 A MX 2019007444A MX 2019007444 A MX2019007444 A MX 2019007444A MX 2019007444 A MX2019007444 A MX 2019007444A
Authority
MX
Mexico
Prior art keywords
methods
nucleic acid
systems
analysis
acid molecules
Prior art date
Application number
MX2019007444A
Other languages
English (en)
Inventor
Abdueva Diana
Kennedy Andrew
Ann Ward MORTIMER Stefanie
Eltoukhy Helmy
Talasaz Amirali
Schultz Matthew
Original Assignee
Guardant Health Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guardant Health Inc filed Critical Guardant Health Inc
Publication of MX2019007444A publication Critical patent/MX2019007444A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La descripción proporciona métodos para procesar poblaciones de ácidos nucleicos que contienen diferentes formas (por ejemplo, ARN y ADN, de hebra individual o doble hebra) y/o grados de modificación (por ejemplo, metilación de citosina, asociación con proteínas). Estos métodos se adaptan a múltiples formas y/o modificaciones de ácido nucleico en una muestra, de manera que se puede obtener información de secuencia para múltiples formas. Los métodos también preservan identidad de múltiples formas o estados modificados a través de procesamiento y análisis, de manera que el análisis de secuencia se puede combinar con análisis epigenético.
MX2019007444A 2016-12-22 2017-12-22 Metodos y sistemas para analisis de moleculas de acido nucleico. MX2019007444A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662438240P 2016-12-22 2016-12-22
US201762512936P 2017-05-31 2017-05-31
US201762550540P 2017-08-25 2017-08-25
PCT/US2017/068329 WO2018119452A2 (en) 2016-12-22 2017-12-22 Methods and systems for analyzing nucleic acid molecules

Publications (1)

Publication Number Publication Date
MX2019007444A true MX2019007444A (es) 2019-08-16

Family

ID=61006366

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019007444A MX2019007444A (es) 2016-12-22 2017-12-22 Metodos y sistemas para analisis de moleculas de acido nucleico.

Country Status (11)

Country Link
US (2) US11519019B2 (es)
EP (1) EP3559270A2 (es)
JP (2) JP7300989B2 (es)
KR (1) KR20190095410A (es)
CN (1) CN110325650A (es)
AU (1) AU2017382439A1 (es)
BR (1) BR112019012958A2 (es)
CA (1) CA3046007A1 (es)
IL (2) IL302912A (es)
MX (1) MX2019007444A (es)
WO (1) WO2018119452A2 (es)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
CN104364392B (zh) 2012-02-27 2018-05-25 赛卢拉研究公司 用于分子计数的组合物和试剂盒
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
KR20230074639A (ko) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
JP7091348B2 (ja) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー バーコード付きオリゴヌクレオチド配列を有する試薬を用いたタンパク質発現の測定
US10697008B2 (en) 2017-04-12 2020-06-30 Karius, Inc. Sample preparation methods, systems and compositions
CA3072195A1 (en) 2017-08-07 2019-04-04 The Johns Hopkins University Methods and materials for assessing and treating cancer
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
SG11202100344WA (en) 2018-07-23 2021-02-25 Guardant Health Inc Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
US20200113505A1 (en) 2018-10-11 2020-04-16 Seno Medical Instruments, Inc. Optoacoustic image analysis method and system for automatically estimating lesion traits
CN113286881A (zh) 2018-09-27 2021-08-20 格里尔公司 甲基化标记和标靶甲基化探针板
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
SG11202104701XA (en) * 2018-12-20 2021-06-29 Guardant Health Inc Methods, compositions, and systems for improving recovery of nucleic acid molecules
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
EP3914736B1 (en) * 2019-01-25 2023-12-20 Grail, LLC Detecting cancer, cancer tissue of origin, and/or a cancer cell type
CN113661249A (zh) * 2019-01-31 2021-11-16 夸登特健康公司 用于分离无细胞dna的组合物和方法
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
EP3969583A1 (en) * 2019-05-14 2022-03-23 F. Hoffmann-La Roche AG Devices and methods for sample analysis
WO2020243722A1 (en) * 2019-05-31 2020-12-03 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
EP4017977A1 (en) * 2019-08-19 2022-06-29 F. Hoffmann-La Roche AG Single tube preparation of dna and rna for sequencing
US11891653B2 (en) 2019-09-30 2024-02-06 Guardant Health, Inc. Compositions and methods for analyzing cell-free DNA in methylation partitioning assays
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
US11898199B2 (en) 2019-11-11 2024-02-13 Universal Diagnostics, S.A. Detection of colorectal cancer and/or advanced adenomas
CN114746560A (zh) * 2019-11-26 2022-07-12 夸登特健康公司 改进甲基化多核苷酸结合的方法、组合物和系统
CN115151657A (zh) * 2019-12-20 2022-10-04 安可济控股有限公司 用于疾病检测的方法和系统
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
GB202000747D0 (en) * 2020-01-17 2020-03-04 Institute Of Cancer Res Monitoring tumour evolution
WO2021202752A1 (en) * 2020-03-31 2021-10-07 Guardant Health, Inc. Determining tumor fraction for a sample based on methyl binding domain calibration data
EP4143338A1 (en) 2020-04-30 2023-03-08 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
CN115715330A (zh) * 2020-05-12 2023-02-24 小利兰·斯坦福大学托管委员会 用于从无细胞dna中推断基因表达和起源组织的系统和方法
EP4150113A1 (en) 2020-05-14 2023-03-22 Guardant Health, Inc. Homologous recombination repair deficiency detection
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
AU2021276524A1 (en) * 2020-05-22 2023-01-05 Aqtual, Inc. Methods for characterizing cell-free nucleic acid fragments
EP4150121A1 (en) 2020-06-30 2023-03-22 Universal Diagnostics, S.A. Systems and methods for detection of multiple cancer types
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN111826430A (zh) * 2020-07-24 2020-10-27 扬州大学 一种研究鸡PGCs中lncRNA和组蛋白甲基化酶共调控靶基因的方法
US11946044B2 (en) * 2020-07-30 2024-04-02 Guardant Health, Inc. Methods for isolating cell-free DNA
JP2023540221A (ja) 2020-08-25 2023-09-22 ガーダント ヘルス， インコーポレイテッド バリアントの起源を予測するための方法およびシステム
WO2022073012A1 (en) 2020-09-30 2022-04-07 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and a methylation-dependent nuclease
US20220154286A1 (en) 2020-10-23 2022-05-19 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and base conversion
WO2022108946A1 (en) 2020-11-17 2022-05-27 Becton, Dickinson And Company Combined analysis of cell-free nucleic acids and single cells for oncology diagnostics
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CA3199829A1 (en) 2020-11-30 2022-06-02 Guardant Health, Inc. Compositions and methods for enriching methylated polynucleotides
WO2022140629A1 (en) * 2020-12-23 2022-06-30 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
EP4291679A1 (en) 2021-02-12 2023-12-20 Guardant Health, Inc. Methods and compositions for detecting nucleic acid variants
AU2022231055A1 (en) 2021-03-05 2023-09-14 Guardant Health, Inc. Methods and related aspects for analyzing molecular response
WO2022187867A1 (en) * 2021-03-05 2022-09-09 The Trustees Of Columbia University In The City Of New York Methods to analyze methylomes in tumor and plasma cell-free dna
EP4305200A1 (en) 2021-03-09 2024-01-17 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
WO2022192189A1 (en) * 2021-03-09 2022-09-15 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP4314329A1 (en) 2021-03-25 2024-02-07 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna
WO2022226389A1 (en) * 2021-04-23 2022-10-27 The Translational Genomics Research Institute Analysis of fragment ends in dna
WO2022251655A1 (en) 2021-05-28 2022-12-01 Guardant Health, Inc. Compositions and methods for assaying circulating molecules
WO2022271730A1 (en) 2021-06-21 2022-12-29 Guardant Health, Inc. Methods and compositions for copy-number informed tissue-of-origin analysis
CA3225385A1 (en) * 2021-07-12 2023-01-19 The Trustees Of The University Of Pennsylvania Modified adapters for enzymatic dna deamination and methods of use thereof for epigenetic sequencing of free and immobilized dna
WO2023056065A1 (en) 2021-09-30 2023-04-06 Guardant Health, Inc. Compositions and methods for synthesis and use of probes targeting nucleic acid rearrangements
WO2023081722A2 (en) 2021-11-02 2023-05-11 Guardant Health, Inc. Quality control method
WO2023086967A1 (en) 2021-11-12 2023-05-19 Guardant Health, Inc. Method of analysis of methylated dna-binding proteins
WO2023122623A1 (en) 2021-12-21 2023-06-29 Guardant Health, Inc. Methods and systems for combinatorial chromatin-ip sequencing
WO2023122740A1 (en) 2021-12-23 2023-06-29 Guardant Health, Inc. Compositions and methods for detection of metastasis
WO2023197004A1 (en) 2022-04-07 2023-10-12 Guardant Health, Inc. Detecting the presence of a tumor based on methylation status of cell-free nucleic acid molecules
WO2024006908A1 (en) 2022-06-30 2024-01-04 Guardant Health, Inc. Enrichment of aberrantly methylated dna
WO2024020573A1 (en) 2022-07-21 2024-01-25 Guardant Health, Inc. Methods for detection and reduction of sample preparation-induced methylation artifacts
WO2024040006A2 (en) * 2022-08-15 2024-02-22 Bioscreening & Diagnostics Llc Ai and ml-based system to predict cancer from epigenetic data
WO2024059840A1 (en) 2022-09-16 2024-03-21 Guardant Health, Inc. Compositions and methods for analyzing soluble proteins
WO2024073508A2 (en) 2022-09-27 2024-04-04 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US7208271B2 (en) 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) * 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
WO2010037001A2 (en) * 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
US20130157266A1 (en) * 2009-03-15 2013-06-20 Ribomed Biotechnologies, Inc. Abscription based molecular detection of dna methylation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3425065B1 (en) * 2011-12-13 2021-04-21 Oslo Universitetssykehus HF Methods and kits for detection of methylation status
WO2013142389A1 (en) * 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US10087485B2 (en) * 2012-06-06 2018-10-02 The Trustees Of Princeton University DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
IL305303A (en) 2012-09-04 2023-10-01 Guardant Health Inc Systems and methods for detecting rare mutations and changes in number of copies
US10612088B2 (en) 2013-03-14 2020-04-07 The Broad Institute, Inc. Massively multiplexed RNA sequencing
WO2014167564A1 (en) * 2013-04-08 2014-10-16 Carmel-Haifa University Economic Corporation Ltd. Sept4/ARTS AS A TUMOR SUPPRESSOR IN THE DIAGNOSIS, PROGNOSIS AND TREATMENT OF HEPATIC DISORDERS
EP2805769A1 (en) * 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
AU2014340239B2 (en) 2013-10-21 2019-11-28 Verinata Health, Inc. Method for improving the sensitivity of detection in determining copy number variations
EP4306659A3 (en) 2014-04-14 2024-03-27 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
CA2956208A1 (en) 2014-07-25 2016-01-28 University Of Washington Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
ES2925014T3 (es) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
US9984201B2 (en) * 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
EP3443066A4 (en) 2016-04-14 2019-12-11 Guardant Health, Inc. EARLY DETECTION METHODS FOR CANCER
WO2017181161A1 (en) * 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
US20190127789A1 (en) * 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
CN109415763A (zh) 2016-05-03 2019-03-01 大学健康网络 捕获无细胞的甲基化dna的方法及其用途
CA3024984C (en) * 2016-06-30 2021-12-07 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
AU2017292854B2 (en) 2016-07-06 2023-08-17 Guardant Health, Inc. Methods for fragmentome profiling of cell-free nucleic acids
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
KR102628878B1 (ko) 2017-07-12 2024-01-23 유니버시티 헬스 네트워크 메틸롬 분석을 이용한 암 검출 및 분류

Also Published As

Publication number Publication date
BR112019012958A2 (pt) 2019-11-26
US11952616B2 (en) 2024-04-09
CN110325650A (zh) 2019-10-11
IL267424A (en) 2019-08-29
IL302912A (en) 2023-07-01
US11519019B2 (en) 2022-12-06
JP2020504606A (ja) 2020-02-13
WO2018119452A2 (en) 2018-06-28
CA3046007A1 (en) 2018-06-28
JP2023089062A (ja) 2023-06-27
US20230332206A1 (en) 2023-10-19
AU2017382439A1 (en) 2019-06-20
JP7300989B2 (ja) 2023-06-30
WO2018119452A3 (en) 2018-08-09
KR20190095410A (ko) 2019-08-14
EP3559270A2 (en) 2019-10-30
US20190390253A1 (en) 2019-12-26

Similar Documents

Publication Publication Date Title
MX2019007444A (es) Metodos y sistemas para analisis de moleculas de acido nucleico.
GB2568608A (en) Personalized genetic testing
MY195527A (en) Methods And Systems For Tumor Detection
MX2016016902A (es) Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2018119447A3 (en) Methods and systems for processing polynucleotides
GB201319347D0 (en) Compositions and kits for molecular counting
MY197535A (en) Diagnostic applications using nucleic acid fragments
MY170164A (en) Aptamer-based multiplexed assays
IL260118B (en) "Infragenomic structure for on-site or cloud-based processing and analysis of dna and rna"
EP3305918A3 (en) Systems and methods for epigenetic sequencing
WO2008093098A3 (en) Methods for indexing samples and sequencing multiple nucleotide templates
WO2017065959A3 (en) Methods and compositions that utilize transcriptome sequencing data in machine learning-based classification
WO2015200717A3 (en) Pcr-activated sorting (pas)
MY182940A (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
MX2015012319A (es) Sistemas de instrumento analitico.
EP4254417A3 (en) Using nucleic acid size range for noninvasive prenatal testing and cancer detection
WO2016127077A3 (en) Systems and methods for detection and analysis of biological species
TR201903963T4 (tr) Hizalama içermeyen sekans analizi kullanılarak bir hedef sekans içindeki mutasyon(lar)ı içeren üyeler için bir popülasyonun taranmasına ait yüksek verimlilik metodu.
WO2018195452A3 (en) FLUID-SEALED FLOW SYSTEM FOR ISOLATING BIOMARKERS FROM A LIQUID SAMPLE
BR112021018125A2 (pt) Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado
GB202403752D0 (en) Methods and systems for analyzing nucleic acid molecules
WO2020005846A8 (en) Labeling of dna
EP3314024A4 (en) METHOD AND SYSTEM FOR PROFILING MULTI-TARGET CHROMOSOMES IN BIOLOGICAL SAMPLES USING TARGET-SPECIFIC DNA PROBES
WO2016051177A3 (en) Methods and kits
WO2018223057A8 (en) Array-based methods for analysing mixed samples using different allele-specific labels, in particular for detection of fetal aneuploidies