BR112021018125A2 - Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado - Google Patents

Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado

Info

Publication number
BR112021018125A2
BR112021018125A2 BR112021018125A BR112021018125A BR112021018125A2 BR 112021018125 A2 BR112021018125 A2 BR 112021018125A2 BR 112021018125 A BR112021018125 A BR 112021018125A BR 112021018125 A BR112021018125 A BR 112021018125A BR 112021018125 A2 BR112021018125 A2 BR 112021018125A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
generating
stranded nucleic
acid template
tagged dna
Prior art date
Application number
BR112021018125A
Other languages
English (en)
Inventor
Anthony Gormley Niall
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of BR112021018125A2 publication Critical patent/BR112021018125A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/113—Telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado. um método para gerar um molde de ácido nucleico de fita dupla assimétrico de extremidades fechadas a partir de um molde de ácido nucleico de fita dupla que tem extremidades 5 e 3 livres através do uso de adaptadores em forma de grampo ou haltere e o sequenciamento dos mesmos.
BR112021018125A 2019-10-25 2020-10-23 Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado BR112021018125A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962926360P 2019-10-25 2019-10-25
PCT/EP2020/079902 WO2021078947A1 (en) 2019-10-25 2020-10-23 Methods for generating, and sequencing from, asymmetric adaptors on the ends of polynucleotide templates comprising hairpin loops

Publications (1)

Publication Number Publication Date
BR112021018125A2 true BR112021018125A2 (pt) 2021-11-16

Family

ID=73020204

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021018125A BR112021018125A2 (pt) 2019-10-25 2020-10-23 Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado

Country Status (12)

Country Link
US (1) US20220259587A1 (pt)
EP (1) EP3918066A1 (pt)
JP (1) JP2022553471A (pt)
KR (1) KR20220088632A (pt)
CN (1) CN113939590A (pt)
AU (1) AU2020370740A1 (pt)
BR (1) BR112021018125A2 (pt)
CA (1) CA3131183A1 (pt)
IL (1) IL286593A (pt)
MX (1) MX2021010579A (pt)
SG (1) SG11202110571YA (pt)
WO (1) WO2021078947A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023137667A1 (zh) * 2022-01-20 2023-07-27 深圳华大智造科技股份有限公司 一种接头及其在构建dnb文库中的应用
WO2023220729A2 (en) * 2022-05-13 2023-11-16 Flagship Pioneering Innovations Vii, Llc Double stranded dna compositions and related methods
WO2024020410A1 (en) * 2022-07-22 2024-01-25 Pacific Biosciences Of California, Inc. Systems and methods for dual-end sequencing
WO2024121354A1 (en) * 2022-12-08 2024-06-13 Keygene N.V. Duplex sequencing with covalently closed dna ends

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
CA2160016C (en) 1993-04-12 2008-06-03 Robert L. Letsinger Method of forming oligonucleotides
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US20050191698A1 (en) 1999-04-20 2005-09-01 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
EP2092322B1 (en) 2006-12-14 2016-02-17 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP3092308A1 (en) * 2014-01-07 2016-11-16 Fundacio Privada Institut de Medicina Predictiva i Personalitzada del Cancer Method for generating double stranded dna libraries and sequencing methods for the identification of methylated cytosines
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
EP3485037B1 (en) * 2016-07-18 2022-02-09 F. Hoffmann-La Roche AG Asymmetric templates and asymmetric method of nucleic acid sequencing
EP3500682B1 (en) * 2016-08-16 2020-07-08 Touchlight IP Limited Closed linear dna production
US10711269B2 (en) * 2017-01-18 2020-07-14 Agilent Technologies, Inc. Method for making an asymmetrically-tagged sequencing library

Also Published As

Publication number Publication date
US20220259587A1 (en) 2022-08-18
KR20220088632A (ko) 2022-06-28
SG11202110571YA (en) 2021-10-28
IL286593A (en) 2021-12-01
AU2020370740A1 (en) 2021-09-30
CN113939590A (zh) 2022-01-14
CA3131183A1 (en) 2021-04-29
EP3918066A1 (en) 2021-12-08
JP2022553471A (ja) 2022-12-23
WO2021078947A1 (en) 2021-04-29
MX2021010579A (es) 2021-11-12

Similar Documents

Publication Publication Date Title
BR112021018125A2 (pt) Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado
MX2022010611A (es) Identificacion libre de bisulfito de modificaciones de citosina por resolucion de base.
WO2018119452A3 (en) Methods and systems for analyzing nucleic acid molecules
BR112019006388A2 (pt) enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas
MX2020004578A (es) Composiciones de casz y metodos de uso.
WO2018135838A3 (ko) Dna 단일가닥 절단에 의한 염기 교정 비표적 위치 확인 방법
WO2016015058A3 (en) Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
GB2568608A (en) Personalized genetic testing
BR112017023189A2 (pt) aparelho de efeito de campo e métodos para sequenciar ácidos nucleicos
BR112015023489A2 (pt) uso de rnas-guia truncads (tru - grnas) para aumentar a especificidade de edição de genoma com rna guiado
BR112018013550A2 (pt) identificação de entidades utilizando um modelo de aprendizado profundo
WO2017201102A8 (en) Method of improved sequencing by strand identification
WO2019135816A3 (en) Novel nucleic acid modifiers
ATE514775T1 (de) Endenmodifikation zur verhinderung einer überrepräsentierung von fragmenten
CL2021000028A1 (es) Anticuerpo multiespecifico (divisional de solicitud 937-2020).
EA201692157A1 (ru) Синтез двухцепочечных нуклеиновых кислот
BR112019005810A2 (pt) método para sequenciamento de microbioma melhorado para caracterizar uma afecção relacionada a micro-organismo para um usuário associado a uma amostra biológica, e método para sequenciamento de microbioma melhorado
MX2021008487A (es) Escisión y poliadenilación del arn nuclear dirigido con crispr-cas.
MX2018009251A (es) Tubo fragmentador de microesferas y metodo para extraer acido desoxirribonucleico y/o acido ribonucleico de microorganismos.
BR112018010639A2 (pt) método de transfecção transitória para produção retroviral.
BR112019005025A8 (pt) Sistema e método para caracterizar um painel
CY1122030T1 (el) Μεθοδος εκτιμησης της ποσοτητας μεθυλιωμενου τοπου σε ενα δειγμα
CO2019003583A2 (es) Estructuras eficientes de datos para la representación de información bioinformática
WO2020005846A8 (en) Labeling of dna
WO2003078603A3 (en) Strong gene sets for glioma classification