MX2021008487A - Escisión y poliadenilación del arn nuclear dirigido con crispr-cas. - Google Patents

Escisión y poliadenilación del arn nuclear dirigido con crispr-cas.

Info

Publication number
MX2021008487A
MX2021008487A MX2021008487A MX2021008487A MX2021008487A MX 2021008487 A MX2021008487 A MX 2021008487A MX 2021008487 A MX2021008487 A MX 2021008487A MX 2021008487 A MX2021008487 A MX 2021008487A MX 2021008487 A MX2021008487 A MX 2021008487A
Authority
MX
Mexico
Prior art keywords
crispr
polyadenylation
cas
rna cleavage
nuclear rna
Prior art date
Application number
MX2021008487A
Other languages
English (en)
Inventor
Douglas Matthew Anderson
Original Assignee
Univ Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rochester filed Critical Univ Rochester
Publication of MX2021008487A publication Critical patent/MX2021008487A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Abstract

La presente invención proporciona proteínas, ácidos nucleicos, sistemas y métodos para visualizar o modular el ARN.
MX2021008487A 2019-01-14 2020-01-14 Escisión y poliadenilación del arn nuclear dirigido con crispr-cas. MX2021008487A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962791971P 2019-01-14 2019-01-14
US201962877415P 2019-07-23 2019-07-23
PCT/US2020/013577 WO2020150287A1 (en) 2019-01-14 2020-01-14 Targeted nuclear rna cleavage and polyadenylation with crispr-cas

Publications (1)

Publication Number Publication Date
MX2021008487A true MX2021008487A (es) 2021-11-12

Family

ID=69701472

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021008487A MX2021008487A (es) 2019-01-14 2020-01-14 Escisión y poliadenilación del arn nuclear dirigido con crispr-cas.

Country Status (10)

Country Link
US (1) US20220090088A1 (es)
EP (1) EP3911741A1 (es)
JP (1) JP7416451B2 (es)
KR (1) KR20210126014A (es)
CN (1) CN113544267A (es)
AU (1) AU2020208346A1 (es)
BR (1) BR112021013715A2 (es)
CA (1) CA3126481A1 (es)
MX (1) MX2021008487A (es)
WO (1) WO2020150287A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021195525A1 (en) * 2020-03-27 2021-09-30 University Of Rochester Crispr-cas13 crrna arrays
EP4127169A1 (en) * 2020-03-27 2023-02-08 University of Rochester Targeted destruction of viral rna by crispr-cas13
EP4277987A1 (en) * 2021-02-25 2023-11-22 Duke University Compositions for and methods of engineering the transcriptome
WO2023150131A1 (en) * 2022-02-01 2023-08-10 The Regents Of The University Of California Method of regulating alternative polyadenylation in rna
CN116790555A (zh) * 2022-03-14 2023-09-22 上海鲸奇生物科技有限公司 Rna靶向基因编辑工具的开发
GB202207026D0 (en) * 2022-05-13 2022-06-29 Univ Oxford Innovation Ltd Method
WO2024041653A1 (zh) * 2022-08-26 2024-02-29 广州瑞风生物科技有限公司 一种CRISPR-Cas13系统及其应用
CN115716880A (zh) * 2022-12-07 2023-02-28 云舟生物科技(广州)股份有限公司 一种核定位荧光蛋白及其应用
CN116083400B (zh) * 2022-12-08 2023-12-12 广州瑞风生物科技有限公司 Cas蛋白截短体、构建其的方法及其应用
CN116375818A (zh) * 2023-02-28 2023-07-04 华南农业大学 携带mApple荧光报告基因的重组H5N8亚型禽流感病毒的构建及其应用

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DE3751873T2 (de) 1986-04-09 1997-02-13 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
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US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
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Also Published As

Publication number Publication date
CA3126481A1 (en) 2020-07-23
AU2020208346A1 (en) 2021-07-29
BR112021013715A2 (pt) 2021-09-21
CN113544267A (zh) 2021-10-22
KR20210126014A (ko) 2021-10-19
JP7416451B2 (ja) 2024-01-17
EP3911741A1 (en) 2021-11-24
JP2022523632A (ja) 2022-04-26
US20220090088A1 (en) 2022-03-24
WO2020150287A1 (en) 2020-07-23

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