MX2017011012A - Modificacion de proteina de celula huesped. - Google Patents

Modificacion de proteina de celula huesped.

Info

Publication number
MX2017011012A
MX2017011012A MX2017011012A MX2017011012A MX2017011012A MX 2017011012 A MX2017011012 A MX 2017011012A MX 2017011012 A MX2017011012 A MX 2017011012A MX 2017011012 A MX2017011012 A MX 2017011012A MX 2017011012 A MX2017011012 A MX 2017011012A
Authority
MX
Mexico
Prior art keywords
host cell
cell protein
protein modification
protein
expression
Prior art date
Application number
MX2017011012A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Goren Michael
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55538620&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017011012(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017011012A publication Critical patent/MX2017011012A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporcionan composiciones y métodos para líneas celulares diseñadas y sistemas de expresión que permiten la expresión de proteínas recombinantes en células eucarióticas y su facilidad de aislamiento. También se proporcionan sistemas de expresión de células capaces de expresar una proteína de interés esencialmente libre de una proteína de célula huésped unida.
MX2017011012A 2015-02-27 2016-02-26 Modificacion de proteina de celula huesped. MX2017011012A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562126213P 2015-02-27 2015-02-27
US201662298869P 2016-02-23 2016-02-23
PCT/US2016/019915 WO2016138467A1 (en) 2015-02-27 2016-02-26 Host cell protein modification

Publications (1)

Publication Number Publication Date
MX2017011012A true MX2017011012A (es) 2017-10-20

Family

ID=55538620

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017011012A MX2017011012A (es) 2015-02-27 2016-02-26 Modificacion de proteina de celula huesped.

Country Status (20)

Country Link
US (1) US20160251411A1 (es)
EP (1) EP3262168B1 (es)
JP (1) JP6751402B2 (es)
KR (1) KR20170120126A (es)
CN (1) CN107406831A (es)
AU (1) AU2016224988A1 (es)
BR (1) BR112017018149A2 (es)
CA (1) CA2976669A1 (es)
DK (1) DK3262168T3 (es)
EA (1) EA201791918A1 (es)
ES (1) ES2751670T3 (es)
HK (1) HK1248272A1 (es)
HU (1) HUE045862T2 (es)
IL (1) IL253863A0 (es)
MX (1) MX2017011012A (es)
MY (1) MY180896A (es)
SG (1) SG11201706335TA (es)
TW (1) TW201702380A (es)
WO (1) WO2016138467A1 (es)
ZA (1) ZA201705712B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3504328A1 (en) * 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
JP7116059B2 (ja) 2016-11-21 2022-08-09 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド アフリベルセプト製剤及びその使用
CA3067735A1 (en) * 2017-08-17 2019-02-21 Just Biotherapeutics, Inc. Method of purifying glycosylated protein from host cell galectins and other contaminants
KR20200141473A (ko) * 2018-05-04 2020-12-18 시그마-알드리치 컴퍼니., 엘엘씨 숙주 세포 단백질의 감소된 수준을 갖는 재조합 단백질 생산
JP2021521873A (ja) * 2018-05-04 2021-08-30 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ 改変された宿主細胞タンパク質プロファイルを有する操作された細胞
US20210096130A1 (en) * 2019-09-12 2021-04-01 Lonza Ltd Compositions, methods, and kits for detection of lipolytic activity
EP4045641A1 (en) 2019-10-15 2022-08-24 Eli Lilly and Company Recombinantly engineered, lipase/esterase-deficient mammalian cell lines

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4091119B2 (ja) * 1996-09-19 2008-05-28 ノボザイムス アクティーゼルスカブ 新規な宿主細胞およびタンパク質生産方法
PL2150617T3 (pl) * 2007-06-04 2015-04-30 Regeneron Pharma Wzmocniona ekspresja i regiony stabilności
GB201000587D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
SG10201606218WA (en) * 2011-07-29 2016-09-29 Eleven Biotherapeutics Inc Purified Proteins
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
KR102332302B1 (ko) * 2013-09-13 2021-12-01 제넨테크, 인크. 세포주에서 숙주 세포 단백질 및 재조합 폴리펩티드 생성물을 검출 및 정량화하기 위한 조성물 및 방법
EP3683232A1 (en) 2013-09-13 2020-07-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
US9932591B2 (en) * 2013-12-18 2018-04-03 University Of Delaware Reduction of lipase activity in product formulations

Also Published As

Publication number Publication date
KR20170120126A (ko) 2017-10-30
JP2018506295A (ja) 2018-03-08
CN107406831A (zh) 2017-11-28
EP3262168A1 (en) 2018-01-03
CA2976669A1 (en) 2016-09-01
AU2016224988A1 (en) 2017-09-07
HK1248272A1 (zh) 2018-10-12
HUE045862T2 (hu) 2020-01-28
ZA201705712B (en) 2020-01-29
MY180896A (en) 2020-12-11
SG11201706335TA (en) 2017-09-28
ES2751670T3 (es) 2020-04-01
WO2016138467A1 (en) 2016-09-01
JP6751402B2 (ja) 2020-09-02
IL253863A0 (en) 2017-09-28
BR112017018149A2 (pt) 2018-04-10
TW201702380A (zh) 2017-01-16
EA201791918A1 (ru) 2017-12-29
EP3262168B1 (en) 2019-08-21
DK3262168T3 (da) 2019-10-07
US20160251411A1 (en) 2016-09-01

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