MX2014014784A - Ensayos multiplexados basados en aptámeros. - Google Patents
Ensayos multiplexados basados en aptámeros.Info
- Publication number
- MX2014014784A MX2014014784A MX2014014784A MX2014014784A MX2014014784A MX 2014014784 A MX2014014784 A MX 2014014784A MX 2014014784 A MX2014014784 A MX 2014014784A MX 2014014784 A MX2014014784 A MX 2014014784A MX 2014014784 A MX2014014784 A MX 2014014784A
- Authority
- MX
- Mexico
- Prior art keywords
- aptamer
- target
- kits
- nucleic acid
- detection
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/205—Aptamer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
Abstract
La presente descripción describe métodos, dispositivos, reactivos y Kits para la detección de una o más moléculas objetivo que puedan estar presentes en una muestra de prueba. Los métodos, dispositivos, Kits y reactivos descritos facilitan la detección y cuantificación de un objetivo que no es ácido nucleico (por ejemplo, una proteína objetivo) en una muestra de prueba mediante la detección y cuantificación de un ácido nucleico (es decir, un aptámero) donde las interacciones entre aptámeros se reducen significativamente o se eliminan, a la vez que se mantienen la interacción entre objetivo-aptámero.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261656956P | 2012-06-07 | 2012-06-07 | |
PCT/US2013/044792 WO2013185078A1 (en) | 2012-06-07 | 2013-06-07 | Aptamer-based multiplexed assays |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014014784A true MX2014014784A (es) | 2015-03-19 |
MX356413B MX356413B (es) | 2018-05-29 |
Family
ID=49712701
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014014784A MX356413B (es) | 2012-06-07 | 2013-06-07 | Ensayos multiplexados basados en aptámeros. |
Country Status (22)
Country | Link |
---|---|
US (4) | US20150141259A1 (es) |
EP (1) | EP2859121B1 (es) |
JP (1) | JP6298456B2 (es) |
KR (1) | KR102088381B1 (es) |
CN (2) | CN104508150B (es) |
AU (1) | AU2013271401B2 (es) |
BR (1) | BR112014030298B1 (es) |
CA (1) | CA2873099C (es) |
CY (1) | CY1118748T1 (es) |
ES (1) | ES2617226T3 (es) |
HK (1) | HK1204341A1 (es) |
HR (1) | HRP20170296T1 (es) |
IL (1) | IL235588B (es) |
IN (1) | IN2014KN02647A (es) |
MX (1) | MX356413B (es) |
MY (1) | MY170164A (es) |
NZ (1) | NZ701824A (es) |
PH (1) | PH12014502581A1 (es) |
RU (1) | RU2666989C2 (es) |
SG (2) | SG11201407505XA (es) |
WO (1) | WO2013185078A1 (es) |
ZA (1) | ZA201408409B (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110136099A1 (en) | 2007-01-16 | 2011-06-09 | Somalogic, Inc. | Multiplexed Analyses of Test Samples |
US8975026B2 (en) | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
US7947447B2 (en) | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
CN106133211A (zh) * | 2014-01-14 | 2016-11-16 | 爱普济德生物技术有限公司 | 核酸试剂用于对靶分子进行超灵敏数字检测和定量的用途 |
CN116334088A (zh) | 2014-02-18 | 2023-06-27 | 私募蛋白质体运营有限公司 | 用于检测微生物的组合物和方法 |
EP3146337B1 (en) * | 2014-05-23 | 2020-05-27 | Firefly Bioworks, Inc. | Substrate-mediated reactors for bioassays |
GB2528643A (en) * | 2014-07-08 | 2016-02-03 | Adc Biotechnology Ltd | Method of synthesising ADCs |
KR102003589B1 (ko) * | 2016-03-03 | 2019-07-24 | 성균관대학교산학협력단 | 생화학 분자 검출센서 및 다파장 형광을 이용한 생화학 분자 검출방법 |
CN105779609A (zh) * | 2016-04-15 | 2016-07-20 | 上海伊丽萨生物科技有限公司 | 一种超敏免疫检测方法 |
CA3039057A1 (en) * | 2016-10-13 | 2018-04-19 | Laboratory Corporation Of America Holdings | Devices and methods to reduce interfering compounds in biological samples |
US20190317082A1 (en) * | 2018-04-13 | 2019-10-17 | Fluidigm Canada Inc. | Stabilized cell acquisition for elemental analysis |
JP2021528646A (ja) * | 2018-06-22 | 2021-10-21 | ソマロジック・インコーポレーテッド | 改良されたプロテオミクスマルチプレックスアッセイ |
US11181501B2 (en) | 2018-09-11 | 2021-11-23 | City University Of Hong Kong | Method and an apparatus for determining a presence or an amount of a polyamine or its derivative in a sample |
LU101073B1 (en) * | 2018-12-21 | 2020-06-24 | Luxembourg Inst Science & Tech List | Dna aptamers specific of adenovirus types |
KR102177672B1 (ko) * | 2019-02-01 | 2020-11-12 | 주식회사 압타머사이언스 | 압타머를 이용한 다중(multiplex) PCR 방법 |
KR102113078B1 (ko) * | 2019-07-05 | 2020-05-20 | 광주과학기술원 | 압타머의 선별 방법 |
CN110791551A (zh) * | 2019-09-17 | 2020-02-14 | 北京化工大学 | 一种无载体氯霉素适配体信号放大传感器的建立方法 |
WO2021112559A1 (ko) * | 2019-12-03 | 2021-06-10 | 김성천 | 시료의 표적분자 집단에 대한 프로파일을 얻는 방법 |
CN113624724A (zh) * | 2020-05-07 | 2021-11-09 | 廖世奇 | 一种适配体分子信标对靶分子的多元检测分析方法 |
CN111812313B (zh) * | 2020-06-22 | 2021-10-08 | 北京生物制品研究所有限责任公司 | 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法 |
US11578320B2 (en) | 2021-04-27 | 2023-02-14 | Singular Genomics Systems, Inc. | High density sequencing and multiplexed priming |
KR20220167781A (ko) * | 2021-06-14 | 2022-12-21 | 김성천 | 압타머를 이용하여 시료의 표적 단백질 분자 집단에 대한 프로파일을 얻는 방법 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
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ES2259800T3 (es) | 1990-06-11 | 2006-10-16 | Gilead Sciences, Inc. | Procedimientos de uso de ligandos de acido nucleico. |
US6242246B1 (en) | 1997-12-15 | 2001-06-05 | Somalogic, Inc. | Nucleic acid ligand diagnostic Biochip |
PL202358B1 (pl) * | 1999-11-17 | 2009-06-30 | Roche Diagnostics Gmbh | Sposób wytwarzania kompozycji magnetycznych cząstek szklanych, magnetyczne cząstki szklane, kompozycja magnetycznych cząstek szklanych, zawiesina z magnetycznymi cząstkami szklanymi, probówka z kompozycją lub zawiesiną magnetycznych cząstek szklanych, zestaw części z tą probówką, zastosowanie kompozycji, zawiesiny i zestawu zawierających magnetyczne cząstki szklane, sposób izolowania materiału biologicznego |
US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
DE60326864D1 (de) | 2002-07-24 | 2009-05-07 | Bio Rad Laboratories | Proteininteraktionsdifferenzabbildung |
RU2361193C2 (ru) * | 2004-05-19 | 2009-07-10 | Вп Холдинг, Ллс | Оптический датчик с многослойной плазмонной структурой для усовершенствованного обнаружения химических групп посредством sers |
US7699979B2 (en) * | 2005-01-07 | 2010-04-20 | Board Of Trustees Of The University Of Arkansas | Separation system and efficient capture of contaminants using magnetic nanoparticles |
US7285835B2 (en) * | 2005-02-24 | 2007-10-23 | Freescale Semiconductor, Inc. | Low power magnetoelectronic device structures utilizing enhanced permeability materials |
EP1918372A4 (en) * | 2005-07-05 | 2009-08-12 | Ribomic Inc | NUCLEIC ACID CAPABLE OF BINDING TO IMMUNOGLOBULIN G AND USE THEREOF |
EP1994171B1 (en) * | 2006-01-17 | 2015-03-11 | Somalogic, Inc. | Multiplexed analyses of test samples |
US7855054B2 (en) * | 2007-01-16 | 2010-12-21 | Somalogic, Inc. | Multiplexed analyses of test samples |
US7964356B2 (en) | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
US7947447B2 (en) * | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
EP2336314A1 (en) * | 2007-07-17 | 2011-06-22 | Somalogic, Inc. | Improved selex and photoselex |
US8291501B2 (en) * | 2008-02-08 | 2012-10-16 | Cheng Holdings, Llc | Validation of protected intra-system interconnects for digital rights management in electrical computers and digital data processing systems |
US20110120487A1 (en) * | 2008-05-30 | 2011-05-26 | L'oreal | Hair treatment methods and kits |
AU2009273052B2 (en) * | 2008-07-14 | 2015-09-17 | The University Of Tokyo | Aptamer against IL-17 and use thereof |
US9089152B2 (en) * | 2009-04-13 | 2015-07-28 | TEAGASC—Agriculture and Food Development Authority | Method of producing microbeads |
GB0916700D0 (en) * | 2009-09-23 | 2009-11-04 | Nanoco Technologies Ltd | Semiconductor nanoparticle-based materials |
-
2013
- 2013-06-07 US US14/401,791 patent/US20150141259A1/en not_active Abandoned
- 2013-06-07 CN CN201380040107.2A patent/CN104508150B/zh active Active
- 2013-06-07 IN IN2647KON2014 patent/IN2014KN02647A/en unknown
- 2013-06-07 JP JP2015516253A patent/JP6298456B2/ja active Active
- 2013-06-07 AU AU2013271401A patent/AU2013271401B2/en active Active
- 2013-06-07 WO PCT/US2013/044792 patent/WO2013185078A1/en active Application Filing
- 2013-06-07 SG SG11201407505XA patent/SG11201407505XA/en unknown
- 2013-06-07 MX MX2014014784A patent/MX356413B/es active IP Right Grant
- 2013-06-07 EP EP13801106.9A patent/EP2859121B1/en active Active
- 2013-06-07 KR KR1020147034387A patent/KR102088381B1/ko active IP Right Grant
- 2013-06-07 CA CA2873099A patent/CA2873099C/en active Active
- 2013-06-07 CN CN201810446555.7A patent/CN108614102A/zh active Pending
- 2013-06-07 MY MYPI2014703374A patent/MY170164A/en unknown
- 2013-06-07 BR BR112014030298-7A patent/BR112014030298B1/pt active IP Right Grant
- 2013-06-07 ES ES13801106.9T patent/ES2617226T3/es active Active
- 2013-06-07 NZ NZ701824A patent/NZ701824A/en unknown
- 2013-06-07 RU RU2014152698A patent/RU2666989C2/ru not_active Application Discontinuation
- 2013-06-07 SG SG10201701361WA patent/SG10201701361WA/en unknown
-
2014
- 2014-11-09 IL IL235588A patent/IL235588B/en active IP Right Grant
- 2014-11-17 ZA ZA2014/08409A patent/ZA201408409B/en unknown
- 2014-11-19 PH PH12014502581A patent/PH12014502581A1/en unknown
-
2015
- 2015-05-20 HK HK15104829.2A patent/HK1204341A1/xx unknown
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2017
- 2017-02-22 CY CY20171100249T patent/CY1118748T1/el unknown
- 2017-02-22 HR HRP20170296TT patent/HRP20170296T1/hr unknown
- 2017-03-09 US US15/454,080 patent/US20170176422A1/en not_active Abandoned
- 2017-11-30 US US15/827,441 patent/US20180224444A1/en active Pending
-
2021
- 2021-03-30 US US17/217,006 patent/US20210239692A1/en not_active Abandoned
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