MX2013013065A - Metodos y composiciones para detectar la produccion microbiana de compuestos inmiscibles em agua. - Google Patents

Metodos y composiciones para detectar la produccion microbiana de compuestos inmiscibles em agua.

Info

Publication number
MX2013013065A
MX2013013065A MX2013013065A MX2013013065A MX2013013065A MX 2013013065 A MX2013013065 A MX 2013013065A MX 2013013065 A MX2013013065 A MX 2013013065A MX 2013013065 A MX2013013065 A MX 2013013065A MX 2013013065 A MX2013013065 A MX 2013013065A
Authority
MX
Mexico
Prior art keywords
methods
fluorescent dye
compounds
compositions
detecting
Prior art date
Application number
MX2013013065A
Other languages
English (en)
Inventor
Jeff Ubersax
Lucas Frenz
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2013013065A publication Critical patent/MX2013013065A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporcionan en la presente métodos y composiciones útiles para detectar la producción de compuestos en una célula, por ejemplo, una célula microbiana genéticamente modificada para producir uno o más de tales compuestos en un mayor rendimiento y/o con persistencia incrementada comparada con una célula microbiana original que no se encuentra genéticamente modificada. En algunas modalidades, los métodos comprenden poner en contacto una solución con un tinte fluorescente que enlaza directamente el compuesto producido en forma recombinante, en donde la solución comprende una pluralidad de células que producen en forma recombinante el compuesto; y detectar el tinte fluorescente bajo condiciones espectrales adecuadas para la detección selectiva del tinte fluorescente enlazado al compuesto producido en forma recombinante.
MX2013013065A 2011-05-13 2012-05-10 Metodos y composiciones para detectar la produccion microbiana de compuestos inmiscibles em agua. MX2013013065A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161486211P 2011-05-13 2011-05-13
PCT/US2012/037351 WO2012158466A1 (en) 2011-05-13 2012-05-10 Methods and compositions for detecting microbial production of water-immiscible compounds

Publications (1)

Publication Number Publication Date
MX2013013065A true MX2013013065A (es) 2013-12-02

Family

ID=46148991

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013013065A MX2013013065A (es) 2011-05-13 2012-05-10 Metodos y composiciones para detectar la produccion microbiana de compuestos inmiscibles em agua.

Country Status (11)

Country Link
US (1) US20140057314A1 (es)
EP (1) EP2707722A1 (es)
JP (1) JP2014519028A (es)
KR (1) KR20140032438A (es)
CN (1) CN103518136A (es)
BR (1) BR112013027954A2 (es)
CA (1) CA2834783A1 (es)
MX (1) MX2013013065A (es)
SG (1) SG194785A1 (es)
WO (1) WO2012158466A1 (es)
ZA (1) ZA201307917B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6138597B2 (ja) * 2013-06-07 2017-05-31 株式会社明電舎 嫌気性アンモニア酸化細菌の検出方法
CN110168069A (zh) * 2016-10-19 2019-08-23 通用自动化实验技术公司 用于筛选微生物和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
NL2017905B1 (en) * 2016-12-01 2018-06-18 Biobest Group Nv Beneficial candida yeasts for arthropods
CN109253952A (zh) * 2018-11-08 2019-01-22 深圳市美信检测技术股份有限公司 一种饮用水中微粒的分析方法
NL2023580B1 (en) 2019-07-26 2021-02-18 Isobionics B V Detection of volatiles
TW202248635A (zh) * 2021-04-06 2022-12-16 美商伯克利之光生命科技公司 微流體分析與來自非哺乳動物細胞之生物生產的方法及用於其之套組

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703009A (en) 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes
EP0118367B1 (en) 1983-03-08 1989-04-26 Merck & Co. Inc. Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US5672497A (en) 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
CA1340599C (en) 1986-03-21 1999-06-22 Karen Leigh Cox Antibiotic-producing microorganisms
US5098837A (en) 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
US5149638A (en) 1988-09-29 1992-09-22 Eli Lilly And Company Tylosin biosynthetic genes tylA, tylB and tylI
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
WO1993013663A1 (en) 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5824513A (en) 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
US6060234A (en) 1991-01-17 2000-05-09 Abbott Laboratories Polyketide derivatives and recombinant methods for making same
JPH09504436A (ja) 1993-11-02 1997-05-06 メルク エンド カンパニー インコーポレーテッド トリオールポリケチドシンターゼをコードするｄｎａ
US5716849A (en) 1994-06-08 1998-02-10 Novartis Finance Corporation Genes for the biosynthesis of soraphen
CA2197524A1 (en) 1996-02-22 1997-08-22 Bradley Stuart Dehoff Polyketide synthase genes
CA2197160C (en) 1996-02-22 2007-05-01 Stanley Gene Burgett Platenolide synthase gene
DE69736673T2 (de) 1996-08-20 2007-09-13 Novartis Ag Rifamycin biosynthese genkluster
WO1999005283A2 (fr) 1997-07-25 1999-02-04 Hoechst Marion Roussel Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
US6090601A (en) 1998-01-23 2000-07-18 Kosan Bioscience Sorangium polyketide synthase
US6143526A (en) 1998-03-09 2000-11-07 Baltz; Richard H. Biosynthetic genes for spinosyn insecticide production
NZ509006A (en) 1998-05-28 2003-09-26 Kosan Biosciences Inc Recombinant narbonolide polyketide synthase
NZ508326A (en) 1998-06-18 2003-10-31 Novartis Ag A polyketide synthase and non ribosomal peptide synthase genes, isolated from a myxobacterium, necessary for synthesis of epothiones A and B
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
CN1329668A (zh) 1998-10-02 2002-01-02 高山生物科学股份有限公司 多聚乙酰合成酶及其重组dna构建物
WO2000026349A2 (en) 1998-10-29 2000-05-11 Kosan Biosciences, Inc. Recombinant oleandolide polyketide synthase
NZ511722A (en) 1998-11-20 2004-05-28 Kosan Biosciences Inc Recombinant methods and materials for producing epothilone and epothilone derivatives
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
DK2024504T4 (da) * 2006-05-26 2023-02-27 Amyris Inc Fremstilling af isoprenoider
WO2009005704A1 (en) * 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
BRPI0816951B1 (pt) * 2007-09-20 2023-01-17 Amyris, Inc Método para produzir um composto isoprenóide
CA2724583A1 (en) * 2008-05-16 2009-11-19 The Governors Of The University Of Alberta High throughput methods of identifying neutral lipid synthases
EP2438157A1 (en) 2009-06-01 2012-04-11 Amyris, Inc. Genetically modified microbes producing isoprenoids
ES2464740T3 (es) 2009-06-01 2014-06-03 Amyris, Inc. Método para generar un microbio modificado genéticamente
JP5989098B2 (ja) * 2011-05-09 2016-09-07 アミリス， インコーポレイテッド アセチル−補酵素ａ誘導化合物の産生

Also Published As

Publication number Publication date
CN103518136A (zh) 2014-01-15
EP2707722A1 (en) 2014-03-19
ZA201307917B (en) 2015-01-28
CA2834783A1 (en) 2012-11-22
JP2014519028A (ja) 2014-08-07
US20140057314A1 (en) 2014-02-27
BR112013027954A2 (pt) 2017-01-17
WO2012158466A1 (en) 2012-11-22
KR20140032438A (ko) 2014-03-14
SG194785A1 (en) 2013-12-30

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