MX2011012744A - Metodos novedosos para cuantificar proteinas mediante espectrometria de masas. - Google Patents

Metodos novedosos para cuantificar proteinas mediante espectrometria de masas.

Info

Publication number
MX2011012744A
MX2011012744A MX2011012744A MX2011012744A MX2011012744A MX 2011012744 A MX2011012744 A MX 2011012744A MX 2011012744 A MX2011012744 A MX 2011012744A MX 2011012744 A MX2011012744 A MX 2011012744A MX 2011012744 A MX2011012744 A MX 2011012744A
Authority
MX
Mexico
Prior art keywords
mass spectrometry
novel method
quantifying proteins
sample
amount
Prior art date
Application number
MX2011012744A
Other languages
English (en)
Inventor
Jerome Lemoine
Arnaud Salvador
Jean-Philippe Charrier
Tanguy Fortin
Original Assignee
Biomerieux Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Sa filed Critical Biomerieux Sa
Publication of MX2011012744A publication Critical patent/MX2011012744A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

La presente invención se refiere a un método para la detección cuantitativa de una proteína objetivo en una muestra, en la cual los iones de fragmento de segunda generación se detectan para proporcionar una serie de mediciones cuantitativas, por lo menos una de las cuales se correlaciona con la cantidad de péptido proteotípico generada y con la cantidad de proteína objetivo en la muestra, en donde el ion de fragmento de primera generación seleccionado que tiene una masa (m/z)2 es un péptido doblemente cargado que tiene una prolina y/o una histidina en la posición 1.
MX2011012744A 2009-05-29 2010-05-21 Metodos novedosos para cuantificar proteinas mediante espectrometria de masas. MX2011012744A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0953576A FR2946147B1 (fr) 2009-05-29 2009-05-29 Nouveau procede de quantification de proteines par spectrometrie de masse
PCT/FR2010/050991 WO2010136706A1 (fr) 2009-05-29 2010-05-21 Nouveau procede de quantification de proteines par spectrometrie de masse

Publications (1)

Publication Number Publication Date
MX2011012744A true MX2011012744A (es) 2012-05-22

Family

ID=41584453

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011012744A MX2011012744A (es) 2009-05-29 2010-05-21 Metodos novedosos para cuantificar proteinas mediante espectrometria de masas.

Country Status (10)

Country Link
US (1) US9170263B2 (es)
EP (1) EP2435835B1 (es)
JP (1) JP5567662B2 (es)
KR (1) KR20130027401A (es)
CN (1) CN102483420B (es)
BR (1) BRPI1016065A2 (es)
FR (1) FR2946147B1 (es)
MX (1) MX2011012744A (es)
RU (1) RU2011151478A (es)
WO (1) WO2010136706A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9506167B2 (en) 2011-07-29 2016-11-29 Ginkgo Bioworks, Inc. Methods and systems for cell state quantification
JP6148540B2 (ja) * 2013-06-07 2017-06-14 株式会社島津製作所 質量分析装置を用いたグラニュリンペプチドの定量分析方法、および分析用プログラム
RU2595835C2 (ru) * 2013-07-26 2016-08-27 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича" Российской академии медицинских наук (ФГБУ "ИБМХ" РАМН) Способ получения аналитической тест-системы для мультиплексной индентификации и количественного измерения содержания интересующих белков в биологическом образце по содержанию соответствующих им протеотипических маркерных пептидов
WO2017027796A1 (en) 2015-08-13 2017-02-16 Waters Technologies Corporation Nickel-cobalt alloy material devices and components
CN108604525B (zh) * 2015-12-01 2020-08-07 Dh科技发展私人贸易有限公司 用于靶向ms方法中的自适应保留时间的前哨信号
CN105572265A (zh) * 2016-02-29 2016-05-11 吉林大学 一种单克隆抗体药物的药代动力学质谱分析方法
CN108362760A (zh) * 2017-05-10 2018-08-03 中国科学院过程工程研究所 一种含氧酸根的定性以及半定量检测方法、一种含氧酸根的监测方法及其用途
CN108362759A (zh) * 2017-05-10 2018-08-03 中国科学院过程工程研究所 一种含氧酸根监测系统
CN109900815B (zh) * 2017-12-08 2021-06-29 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
FR3103197A1 (fr) 2019-11-15 2021-05-21 bioMérieux Determination par spectrometrie de masse de la sensibilite ou de la resistance de bacteries a un antibiotique

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077616A1 (en) * 2001-04-19 2003-04-24 Ciphergen Biosystems, Inc. Biomolecule characterization using mass spectrometry and affinity tags
CA2481777C (en) * 2002-04-29 2012-08-07 Mds Inc., Doing Business As Mds Sciex Broad ion fragmentation coverage in mass spectrometry by varying the collision energy
US20070269895A1 (en) 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US6621074B1 (en) * 2002-07-18 2003-09-16 Perseptive Biosystems, Inc. Tandem time-of-flight mass spectrometer with improved performance for determining molecular structure
US7501286B2 (en) 2002-08-14 2009-03-10 President And Fellows Of Harvard College Absolute quantification of proteins and modified forms thereof by multistage mass spectrometry
US7632686B2 (en) 2002-10-03 2009-12-15 Anderson Forschung Group High sensitivity quantitation of peptides by mass spectrometry
WO2005098071A1 (ja) 2004-04-08 2005-10-20 Tohoku Techno Arch Co., Ltd. 水素処理により合金の結晶粒を微細化する方法
DE602005004902T2 (de) 2005-06-02 2008-10-16 Entelechon Gmbh Künstliches Protein, Verfahren für absolute Quantifizierung der Proteine und seine Benutzung
CN101042377A (zh) * 2006-03-20 2007-09-26 中国人民解放军军事医学科学院放射与辐射医学研究所 一种用于含组氨酸肽段富集与测序的方法和试剂盒
WO2008146100A1 (en) 2007-06-01 2008-12-04 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for absolute quantification of polypeptides
GB0809488D0 (en) * 2008-05-23 2008-07-02 Electrophoretics Ltd Mass spectrometric analysis

Also Published As

Publication number Publication date
EP2435835A1 (fr) 2012-04-04
EP2435835B1 (fr) 2013-08-28
JP2012528313A (ja) 2012-11-12
CN102483420B (zh) 2014-07-02
US9170263B2 (en) 2015-10-27
FR2946147A1 (fr) 2010-12-03
BRPI1016065A2 (pt) 2016-05-10
FR2946147B1 (fr) 2012-08-31
WO2010136706A1 (fr) 2010-12-02
CN102483420A (zh) 2012-05-30
US20120208224A1 (en) 2012-08-16
RU2011151478A (ru) 2013-07-10
JP5567662B2 (ja) 2014-08-06
KR20130027401A (ko) 2013-03-15

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