MX2009011805A - Mejora de las concentraciones de polipeptido del factor viii en cultivos celulares. - Google Patents

Mejora de las concentraciones de polipeptido del factor viii en cultivos celulares.

Info

Publication number
MX2009011805A
MX2009011805A MX2009011805A MX2009011805A MX2009011805A MX 2009011805 A MX2009011805 A MX 2009011805A MX 2009011805 A MX2009011805 A MX 2009011805A MX 2009011805 A MX2009011805 A MX 2009011805A MX 2009011805 A MX2009011805 A MX 2009011805A
Authority
MX
Mexico
Prior art keywords
factor viii
viii polypeptide
improvement
cell cultures
titers
Prior art date
Application number
MX2009011805A
Other languages
English (en)
Inventor
Thomas Dock Steenstrup
Laust Bruun Johnsen
Ida Hilden
Gert Bolt
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38519867&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2009011805(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of MX2009011805A publication Critical patent/MX2009011805A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención se refiere a un método para la producción de un polipéptido del Factor VIII, el método comprende las etapas de a) cultivar una célula de mamífero que expresa un polipéptido del Factor VIII bajo condiciones para la expresión del polipéptido del Factor VIII, las condiciones de cultivo involucran un medio de cultivo celular que comprende un ligando de dominio C2, y b) aislar el polipéptido del Factor VIII expresado de la célula de mamífero por medios apropiados.
MX2009011805A 2007-05-04 2008-04-30 Mejora de las concentraciones de polipeptido del factor viii en cultivos celulares. MX2009011805A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07107477A EP1988101A1 (en) 2007-05-04 2007-05-04 Improvement of factor VIII polypeptide titers in cell cultures
PCT/EP2008/055349 WO2008135501A1 (en) 2007-05-04 2008-04-30 Improvement of factor viii polypeptide titers in cell cultures

Publications (1)

Publication Number Publication Date
MX2009011805A true MX2009011805A (es) 2009-11-13

Family

ID=38519867

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009011805A MX2009011805A (es) 2007-05-04 2008-04-30 Mejora de las concentraciones de polipeptido del factor viii en cultivos celulares.

Country Status (14)

Country Link
US (1) US9982033B2 (es)
EP (2) EP1988101A1 (es)
JP (1) JP5506664B2 (es)
KR (1) KR101594805B1 (es)
CN (1) CN101675073B (es)
AT (1) ATE540975T1 (es)
AU (1) AU2008248627B2 (es)
BR (1) BRPI0811177A2 (es)
CA (1) CA2684951A1 (es)
ES (1) ES2379846T3 (es)
MX (1) MX2009011805A (es)
PL (1) PL2144929T3 (es)
RU (1) RU2477318C2 (es)
WO (1) WO2008135501A1 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
JP5808249B2 (ja) 2008-10-20 2015-11-10 アッヴィ・インコーポレイテッド プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
ES2556454T3 (es) 2010-10-05 2016-01-18 Novo Nordisk Health Care Ag Proceso para producción de proteínas
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US20140308707A1 (en) * 2011-11-21 2014-10-16 Novo Nordisk A/S Method for production of factor viii
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
KR101395736B1 (ko) * 2012-07-10 2014-05-16 아주대학교산학협력단 인간 혈액응고인자 ⅷ 도메인 특이적 항체 및 이의 제조방법
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
RU2710967C9 (ru) * 2014-04-16 2020-08-12 Кмк Биологикс А/С Способ отъемно-доливной ферментации с высокой плотностью клеток
KR102192494B1 (ko) 2014-08-04 2020-12-18 시에스엘 리미티드 인자 viii 제형
SG11201706659WA (en) 2015-03-06 2017-09-28 Csl Behring Recombinant Facility Ag Modified von willebrand factor having improved half-life
EP3558918B1 (en) 2016-12-21 2021-11-10 Solvay Specialty Polymers USA, LLC Method for decreasing the concentration of a metal in a monomer composition comprising bis(benzoyl)benzene
KR20220029733A (ko) 2019-07-04 2022-03-08 체에스엘 베링 렝나우 아게 응고 인자 viii의 시험관내 안정성을 증가시키기 위한 절단된 폰 빌레브란트 인자 (vwf)
EP4058049A1 (en) 2019-11-11 2022-09-21 CSL Behring Lengnau AG Polypeptides for inducing tolerance to factor viii
WO2024110426A1 (en) 2022-11-23 2024-05-30 F. Hoffmann-La Roche Ag Method for increasing recombinant protein expression

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672502A (en) 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
US5250421A (en) * 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
EP0253870B1 (en) 1986-01-03 1993-03-31 Genetics Institute, Inc. Method for producing factor viii:c-type proteins
ATE63335T1 (de) * 1986-07-11 1991-05-15 Miles Inc Herstellung von rekombinantem protein.
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
WO1990002175A1 (en) 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US5679549A (en) * 1995-05-04 1997-10-21 Bayer Corporation Production of recombinant factor VIII in the presence of liposome-like substances of mixed composition
US5952198A (en) * 1995-05-04 1999-09-14 Bayer Corporation Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition
US5851800A (en) 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein
US6458563B1 (en) 1996-06-26 2002-10-01 Emory University Modified factor VIII
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
PL365290A1 (en) * 2001-02-05 2004-12-27 Novo Nordisk Health Care Ag Combined use of factor vii polypeptides and factor ix polypeptides
US7351688B2 (en) * 2003-02-05 2008-04-01 The Research Foundation Of State University Of New York Compositions and methods for less immunogenic protein formulations
US20040229793A1 (en) 2003-02-05 2004-11-18 Balasubramanian Sathyamangalam V. Compositions and methods for less immunogenic protein formulations
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins

Also Published As

Publication number Publication date
AU2008248627B2 (en) 2013-06-06
CA2684951A1 (en) 2008-11-13
US20100120094A1 (en) 2010-05-13
US9982033B2 (en) 2018-05-29
AU2008248627A1 (en) 2008-11-13
EP1988101A1 (en) 2008-11-05
KR101594805B1 (ko) 2016-02-17
BRPI0811177A2 (pt) 2014-10-29
RU2477318C2 (ru) 2013-03-10
RU2009143635A (ru) 2011-06-10
EP2144929A1 (en) 2010-01-20
WO2008135501A1 (en) 2008-11-13
EP2144929B1 (en) 2012-01-11
JP2010525829A (ja) 2010-07-29
JP5506664B2 (ja) 2014-05-28
ATE540975T1 (de) 2012-01-15
CN101675073A (zh) 2010-03-17
KR20100016430A (ko) 2010-02-12
CN101675073B (zh) 2013-12-04
PL2144929T3 (pl) 2012-06-29
ES2379846T3 (es) 2012-05-04

Similar Documents

Publication Publication Date Title
MX2009011805A (es) Mejora de las concentraciones de polipeptido del factor viii en cultivos celulares.
AU2019268194A1 (en) Cell culture system
EP4148121A3 (en) Cell-controlled perfusion in continuous culture
NZ630568A (en) Methods and means for the production of ch3 domain-comprising molecules
NZ598677A (en) Production of proteins in glutamine-free cell culture media
MY156588A (en) Systems using cell culture for production of isoprene
ME01218B (me) Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
MY181513A (en) Cell culture compositions and methods for polypeptide production
EP2346984A4 (en) PERFUSION BIOREACTOR, CELL CULTURE SYSTEMS AND METHODS FOR PRODUCING CELLS AND CELL DERIVED PRODUCTS
NZ597742A (en) Method of producing a polypeptide or virus of interest in a continuous cell culture
WO2010096588A3 (en) Cell culture media containing combinations of proteins
IN2012DN05207A (es)
WO2010089151A8 (en) Bioreactor for the cultivation of mammalian cells
EP2408903A4 (en) PLURIPOTENTE AND MULTIPOTENTE CELL CULTURE ON MICROWAVES
UA92157C2 (ru) Способ продуцирования гормона роста
EP2582792A4 (en) HEART OF CARDIOMYOCYTES WITH DIALYSATED SERUM
NZ591812A (en) Cell culture method to form aggregates
EP3770248A4 (en) CELL CULTURE METHOD, CELL SUPPORT COMPOSITE PRODUCTION METHOD, CULTURED CELLS AND CELL SUPPORT COMPOSITE
WO2014049382A3 (en) Ethylenediamine fermentative production by a recombinant microorganism
EA200900984A1 (ru) Улучшение клеточного роста
NZ597728A (en) Method for obtaining myofibroblasts
CA2865497A1 (en) Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
CA2804683C (en) Fermentation process
UA42324U (ru) Способ повышения регенерационной способности каллюсных культур мягкой пшеницы, стойких к культуральному фильтрату gaeumannomyces graminis var. tritici
UA59121U (uk) Спосіб отримання естрогенів in vitro

Legal Events

Date Code Title Description
FG Grant or registration