MX173709B - Un metodo para desarrollar cristales de enzimas - Google Patents

Un metodo para desarrollar cristales de enzimas

Info

Publication number
MX173709B
MX173709B MX022845A MX2284590A MX173709B MX 173709 B MX173709 B MX 173709B MX 022845 A MX022845 A MX 022845A MX 2284590 A MX2284590 A MX 2284590A MX 173709 B MX173709 B MX 173709B
Authority
MX
Mexico
Prior art keywords
solution
enzyme
crystals
liquid
relatively small
Prior art date
Application number
MX022845A
Other languages
English (en)
Inventor
Kalevi Visuri
Original Assignee
Stabra Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stabra Ag filed Critical Stabra Ag
Publication of MX173709B publication Critical patent/MX173709B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Abstract

La presente invención se refiere a un proceso para producir cristales uniformes relativamente grandes de una enzima cristalizable, que comprende mantener una solución de enzima cristalizable a una temperatura suficientemente fría para inducir la formación de cristales, mantener dicha solución enfriada en una condición sobresaturada mediante la separación de la solución de una corriente de líquido que contiene cristales de enzima relativamente pequeños, calentar dicho líquido para disolver substancialemente los cristales relativamente pequeños, y regresar el líquido con la enzima disuelta a dicha solución para una cristalización ulterior.
MX022845A 1989-10-13 1990-10-15 Un metodo para desarrollar cristales de enzimas MX173709B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/421,137 US5120650A (en) 1989-10-13 1989-10-13 Method for producing crystalline glucose isomerase

Publications (1)

Publication Number Publication Date
MX173709B true MX173709B (es) 1994-03-23

Family

ID=23669310

Family Applications (1)

Application Number Title Priority Date Filing Date
MX022845A MX173709B (es) 1989-10-13 1990-10-15 Un metodo para desarrollar cristales de enzimas

Country Status (12)

Country Link
US (1) US5120650A (es)
EP (1) EP0495817B1 (es)
JP (1) JP2762167B2 (es)
AT (1) ATE135398T1 (es)
AU (1) AU635976B2 (es)
BR (1) BR9007737A (es)
CA (1) CA2066733A1 (es)
DE (1) DE69025949T2 (es)
DK (1) DK0495817T3 (es)
FI (1) FI99025C (es)
MX (1) MX173709B (es)
WO (1) WO1991005857A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI85285C (fi) * 1988-05-13 1992-03-25 Stabra Ag Tvaerbundet, vattenoloesligt glukosisomeras och foerfarande foer framstaellning daerav.
US5618710A (en) * 1990-08-03 1997-04-08 Vertex Pharmaceuticals, Inc. Crosslinked enzyme crystals
US5801022A (en) * 1990-08-03 1998-09-01 Vertex Pharmaceuticals, Incorporated Method of producing a product with crosslinked crystals of thermolysin
JPH04124082A (ja) * 1990-09-14 1992-04-24 Agency Of Ind Science & Technol 結晶形成方法
EP0795009A1 (en) * 1994-12-28 1997-09-17 Genencor International Inc. Enzyme stabilization
AU724448B2 (en) * 1997-03-13 2000-09-21 Shionogi & Co., Ltd. Process for production of L-Methionine gamma-lyase crystals
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
US6500933B1 (en) 1997-09-05 2002-12-31 Altus Biologics Inc. Methods of preparing carbohydrate crosslinked glycoprotein crystals
ATE397013T1 (de) 1997-09-05 2008-06-15 Altus Pharmaceuticals Inc Kohlenhydrat-vernetzte glykoproteinkristalle
US6541606B2 (en) * 1997-12-31 2003-04-01 Altus Biologics Inc. Stabilized protein crystals formulations containing them and methods of making them
WO2007061918A2 (en) * 2005-11-22 2007-05-31 Genencor International, Inc. In situ fructooligosaccharide production and sucrose reduction
US9144602B2 (en) 2008-09-04 2015-09-29 Sciotec Diagnostic Technologies Gmbh Treatment of fructose malabsorption
EP2161034A1 (de) * 2008-09-04 2010-03-10 Sciotec Diagnostic Technologies GmbH Behandlung von Fruktose-Malabsorption
GB201409451D0 (en) 2014-05-28 2014-07-09 Ipabc Ltd Antimicrobial preparations, methods for preparing the same and uses thereof to combat microorganisms

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237231A (en) * 1979-11-13 1980-12-02 Uop Inc. Method of purifying glucose isomerase and a composition for storing same
DK8502857A (es) * 1984-06-25 1985-12-26
US4659667A (en) * 1985-02-26 1987-04-21 Miles Laboratories, Inc. Process to recover crystalline enzymes and crystalline enzymes produced thereby

Also Published As

Publication number Publication date
FI921526L (fi) 1992-04-07
US5120650A (en) 1992-06-09
EP0495817B1 (en) 1996-03-13
JPH05501242A (ja) 1993-03-11
BR9007737A (pt) 1992-08-18
WO1991005857A1 (en) 1991-05-02
AU6512290A (en) 1991-05-16
AU635976B2 (en) 1993-04-08
DK0495817T3 (da) 1996-07-08
EP0495817A1 (en) 1992-07-29
FI921526A0 (fi) 1992-04-07
FI99025B (fi) 1997-06-13
JP2762167B2 (ja) 1998-06-04
ATE135398T1 (de) 1996-03-15
DE69025949D1 (de) 1996-04-18
FI99025C (fi) 1997-09-25
CA2066733A1 (en) 1991-04-14
DE69025949T2 (de) 1996-09-12

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